Isolation and identification of volatile kairomone that affects acarine predatorprey interactions Involvement of host plant in its production

1990 ◽  
Vol 16 (2) ◽  
pp. 381-396 ◽  
Author(s):  
M. Dicke ◽  
T. A. Van Beek ◽  
M. A. Posthumus ◽  
N. Ben Dom ◽  
H. Van Bokhoven ◽  
...  

2019 ◽  
Vol 8 (3) ◽  
Author(s):  
Ristamora Simangunsong ◽  
Rahmawati Rahmawati ◽  
Mukarlina Mukarlina

Rhizosphere fungi play an important role in the process of plant growth and development. This fungi benefits the host plant in increasing it’s growth rate, resistance to pests, diseases and drought. This study aims to determine the genus members of the durian plant rhizosphere (Durio zibethinus Murr.). Sampling of rhizosphere was carried out at Durian Plantation in Bemban Village Sungai Kakap District, Pontianak. Isolation and identification were carried out in the Microbiology Laboratory, Faculty of Mathematics and Natural Sciences, Tanjungpura University in May 2018 until December 2018. The isolation results obtained 8 rhizosphere fungi isolates.There are member of genera Aureobasidium (JRSP1), Monascus (JRSP2), Trichoderma ( JRSP3), Aspergillus (JRS4), Penicillium (JRSP5), Rhizopus (JRSP6), Aspergillus (JRSP7), Penicillium (JRSP8).



2020 ◽  
Vol 32 (10) ◽  
pp. 2486-2488
Author(s):  
Pooja Saini ◽  
Ekta Menghani ◽  
Rekha Mithal

In this work, an ethyl acetate extract of Cuscuta reflexa grown on Nerium oleander host plant was used for the isolation and identification of pure bioactive compounds. Chloroform:methanol was used as mobile phase for flash column chromatography. Five pure bioactive compounds were isolated and two compounds were identified using GC-MS/MS technique. Glycerol-1-palmitate and squalene were identified in Cuscuta reflexa using NIST library on the basis of area percentage.





Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
YJ Lee ◽  
J Kim ◽  
J Lee ◽  
ES Cho ◽  
OS Bang






Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
CJ Ma ◽  
KY Lee ◽  
HK Yang ◽  
JS Yoon ◽  
YC Kim ◽  
...  


2012 ◽  
Vol 6 (2) ◽  
pp. 7-10
Author(s):  
Mohammad Murshed ◽  
Sabeena Shahnaz ◽  
Md. Abdul Malek

Isolation and identification of post operative hospital acquired infection was carried out from July 2008 to December 2008 in Holy Family Red Crescent Medical College Hospital (private hospital). The major pathogen of wound infection was E. coli. A total; of 120 samples were collected from the surrounding environment of post operative room like floor, bed sheets, instruments, dressing materials, catheter, nasogastric and endotracheal tube. E. coli (40%) was the predominant organism followed by S. aureus (24%). DNA fingerprinting analysis using pulsed field gel electreopheresis of XbaI restriction digested genomic DNA showed that clonal relatedness between the two clinical nd environmental isolates were 100%.DOI: http://dx.doi.org/10.3329/bjmm.v6i2.19369 Bangladesh J Med Microbiol 2012; 06(02): 7-10



2020 ◽  
Vol 26 (1-2) ◽  
pp. 73-78
Author(s):  
A Hossen ◽  
MH Rahman ◽  
MZ Ali ◽  
MA Yousuf ◽  
MZ Hassan ◽  
...  

Duck plague (DP) is the most important infectious disease of geese, ducks and free-ranging water birds. The present study was conducted to determine the prevalence of duck plague virus followed by isolation and identification. For these purposes, a total of 155 cloacal swabs samples were collected randomly from duck of different haor areas of Bangladesh including 45 (41 surveillance and 4 clinical) samples from Netrokona; 42 (40 surveillance and 2 clinical) samples from Kishoregonj; 30 samples from Brahmanbaria and 38 samples from Sunamganj. The samples were processed and pooled (1:5 ratio) for initial screening of target polymerase gene of duck plague virus by polymerase chain reaction (PCR) method. All the samples of a positive pool were then tested individually for identifying the individual positive samples. The result showed that out of 155 samples, 41 (26.45%) were found positive in which 17 were from Netrokona, where 15 (36.58%) were from surveillance samples and 2 (50%) were from clinical sample; 16 were from Kishoregonj, where 14 (35%) were from surveillance samples and 2 (100%) were from clinical sample; 2 (6.6%) were from Brahmanbaria and 5 (13.15%) were from Sunamganj. These positive samples were inoculated into 9-10 days embryonated duck eggs (EDE) through chorioallantoic membrane (CAM) route for the isolation of virus. The EDE died earlier was also chilled, and in a similar way, the CAMs were collected and again performed PCR for id entification of virus. Out of 41 PCR positive samples, 26 samples were isolated and reconfirmed by PCR. Subsequently, DPV was isolated in primary duck embryo fibroblasts cell culture and confirmed by observing cytopathic effect (CPE). Bang. J. Livs. Res. Vol. 26 (1&2), 2019: P. 73-78



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