Nocardia otitidiscaviarum infection of a traumatic skin wound

Wounds within the oral mucosa are known to heal more rapidly than skin wounds. Recent studies suggest that differences in the microRNAome profiles may underlie the exceptional healing that occurs in oral mucosa. Here, we test whether skin wound-healing can be accelerating by increasing the levels of oral mucosa-specific microRNAs. A panel of 57 differentially expressed high expresser microRNAs were identified based on our previously published miR-seq dataset of paired skin and oral mucosal wound-healing [Sci. Rep. (2019) 9:7160]. These microRNAs were further grouped into 5 clusters based on their expression patterns, and their differential expression was confirmed by TaqMan-based quantification of LCM-captured epithelial cells from the wound edges. Of these 5 clusters, Cluster IV (consisting of 8 microRNAs, including miR-31) is most intriguing due to its tissue-specific expression pattern and temporal changes during wound-healing. The in vitro functional assays show that ectopic transfection of miR-31 consistently enhanced keratinocyte proliferation and migration. In vivo, miR-31 mimic treatment led to a statistically significant acceleration of wound closure. Our results demonstrate that wound-healing can be enhanced in skin through the overexpression of microRNAs that are highly expressed in the privileged healing response of the oral mucosa.

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187 Improving Awareness of Managing Post-Operative Haemorrhage in Patients Undergoing Thyroid and Parathyroid Surgery: The SCOOP Protocol

British Journal of Surgery ◽

10.1093/bjs/znab134.382 ◽

2021 ◽

Vol 108 (Supplement_2) ◽

Author(s):

S Bhattacharya ◽

J Jegadeeson ◽

J Ramsingh ◽

P Truran

Keyword(s):

Nursing Staff ◽

Simulation Training ◽

Clinical Signs ◽

Skin Wound ◽

Junior Doctors ◽

Parathyroid Surgery ◽

Surgical Department ◽

Life Threatening ◽

Clinical Questions ◽

Life Threatening Complication

Abstract Introduction Post-operative haemorrhage is a rare but potentially life-threatening complication of thyroid surgery and occurs in 1 in 100 patients. Our aim was to assess current levels of awareness of post-operative haemorrhage in the surgical department and to improve confidence in managing this. Method Questionnaires with a combination of clinical questions were distributed amongst nurses, foundation doctors, senior house officers and registrars in the surgical department. Results There was a clear gap in awareness in all grades. The British Association of Endocrine and Thyroid surgeons (BAETS) have guidance on the management of these patients and in particular the acronym SCOOP (Steristrips removed, Cut subcuticular sutures, Open skin wound, Open strap muscles, Pack wound). 18/24 of participants had not heard of the SCOOP protocol. Most nurses (6/12) all junior doctors (8/8) showed lack of confidence in managing patients with suspected bleeding. Conclusions An informative poster was created for relevant clinical areas as per the BAETS recommendation. These posters outlined the steps in the SCOOP acronymas well as the main clinical signs of haemorrhage. BAETS recommend that all first responders, including nursing staff, junior doctors and the crash team should be aware of the SCOOP protocol. Simulation training sessions are in progress for these members of staff.

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A pulsatile release platform based on photo-induced imine-crosslinking hydrogel promotes scarless wound healing

Nature Communications ◽

10.1038/s41467-021-21964-0 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Jian Zhang ◽

Yongjun Zheng ◽

Jimmy Lee ◽

Jieyu Hua ◽

Shilong Li ◽

...

Keyword(s):

Wound Healing ◽

Wound Repair ◽

Transforming Growth Factor ◽

Transforming Growth Factor Β ◽

Skin Wound ◽

Large Animal ◽

Skin Wounds ◽

Tgfβ Signaling ◽

Pulsatile Release ◽

Ecm Extracellular Matrix

AbstractEffective healing of skin wounds is essential for our survival. Although skin has strong regenerative potential, dysfunctional and disfiguring scars can result from aberrant wound repair. Skin scarring involves excessive deposition and misalignment of ECM (extracellular matrix), increased cellularity, and chronic inflammation. Transforming growth factor-β (TGFβ) signaling exerts pleiotropic effects on wound healing by regulating cell proliferation, migration, ECM production, and the immune response. Although blocking TGFβ signaling can reduce tissue fibrosis and scarring, systemic inhibition of TGFβ can lead to significant side effects and inhibit wound re-epithelization. In this study, we develop a wound dressing material based on an integrated photo-crosslinking strategy and a microcapsule platform with pulsatile release of TGF-β inhibitor to achieve spatiotemporal specificity for skin wounds. The material enhances skin wound closure while effectively suppressing scar formation in murine skin wounds and large animal preclinical models. Our study presents a strategy for scarless wound repair.

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Current knowledge of immunomodulation strategies for chronic skin wound repair

Journal of Biomedical Materials Research Part B Applied Biomaterials ◽

10.1002/jbm.b.34921 ◽

2021 ◽

Author(s):

Parisa Heydari ◽

Mahshid Kharaziha ◽

Jaleh Varshosaz ◽

Shaghayegh Haghjooy Javanmard

Keyword(s):

Wound Repair ◽

Current Knowledge ◽

Skin Wound ◽

Chronic Skin

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The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System

Nanomaterials ◽

10.3390/nano11020457 ◽

2021 ◽

Vol 11 (2) ◽

pp. 457

Author(s):

Andreu Blanquer ◽

Jana Musilkova ◽

Elena Filova ◽

Johanka Taborska ◽

Eduard Brynda ◽

...

Keyword(s):

Wound Healing ◽

Endothelial Cells ◽

Chronic Wounds ◽

Human Keratinocytes ◽

Skin Wound ◽

Platelet Lysate ◽

Therapeutic Approaches ◽

Skin Wound Healing ◽

New Therapeutic Approaches ◽

Proliferation And Differentiation

Chronic wounds affect millions of patients worldwide, and it is estimated that this number will increase steadily in the future due to population ageing. The research of new therapeutic approaches to wound healing includes the development of nanofibrous meshes and the use of platelet lysate (PL) to stimulate skin regeneration. This study considers a combination of a degradable electrospun nanofibrous blend of poly(L-lactide-co-ε-caprolactone) and poly(ε-caprolactone) (PLCL/PCL) membranes (NF) and fibrin loaded with various concentrations of PL aimed at the development of bioactive skin wound healing dressings. The cytocompatibility of the NF membranes, as well as the effect of PL, was evaluated in both monocultures and co-cultures of human keratinocytes and human endothelial cells. We determined that the keratinocytes were able to adhere on all the membranes, and their increased proliferation and differentiation was observed on the membranes that contained fibrin with at least 50% of PL (Fbg + PL) after 14 days. With respect to the co-culture experiments, the membranes with fibrin with 20% of PL were observed to enhance the metabolic activity of endothelial cells and their migration, and the proliferation and differentiation of keratinocytes. The results suggest that the newly developed NF combined with fibrin and PL, described in the study, provides a promising dressing for chronic wound healing purposes.

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Sus Scrofa immune tissues as a new source of bioactive substances for skin wound healing

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2020.12.028 ◽

2020 ◽

Author(s):

Alexandr Basov ◽

Liliya Fedulova ◽

Ekaterina Vasilevskaya ◽

Ekaterina Trofimova ◽

Nataliya Murashova ◽

...

Keyword(s):

Wound Healing ◽

Sus Scrofa ◽

Skin Wound ◽

Bioactive Substances ◽

Skin Wound Healing ◽

Immune Tissues

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Fabrication of gelatin/sodium alginate-poly (3-hydroxybutyric acid) bilayer electrospun biosheet as wound healing material in nursing care on pediatric fracture surgery

Journal of Industrial Textiles ◽

10.1177/1528083720976346 ◽

2020 ◽

pp. 152808372097634

Author(s):

Daiqi Jiang ◽

Zaiju Tong ◽

Lingjun Peng ◽

Lingzhi Zhang ◽

Qianzi Ruan ◽

...

Keyword(s):

Wound Healing ◽

Sodium Alginate ◽

Wound Closure ◽

Skin Wound ◽

Tissue Formation ◽

Hydroxybutyric Acid ◽

Physiochemical Properties ◽

Pediatric Fracture ◽

Fracture Surgery

Novel the bilayered electrospun biosheet with rapid cell mimiciking and proliferative efficacy will be suitable for wound healing application. The optimized concentration of gelatin (G) and sodium alginate (A) biosheet with nanofibrous Poly (3-hydroxybutyric acid) (P) as a bilayered elctrospun matrix through electrospinning. The engineered GAP bilayered biosheet involves tissue formation at extra cellular matrix (ECM) which further characterized its function in vitro and invivo. Here we fabricated GAP which exhibit better physiochemical properties, biological and mechanical properties with superior prosomes it enhance air passable at skin wounds. The Bilayered biosheet matrix possess better biocompatibility, cell adherence, fructuous and cell to cell interactions evaluated using cell lines. Furthermore, GAP bilayered matrix regulates growth factors to attain maximum wound closure efficiency during invivo. Thus, the fabricated GAP electrospun biosheet would be a possible wound dressing for skin wound applications.

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