Hormonal parameters in follicular fluid and the fertilization rate of in vitro cultured oocytes

1987 ◽  
Vol 240 (4) ◽  
pp. 207-210 ◽  
Author(s):  
A. Reinthaller ◽  
J. Deutinger ◽  
Ch. Bieglmayer ◽  
P. Riss ◽  
E. Müller-Tyl ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Fertilization Rate

scholarly journals MicroRNA-451 is downregulated in the follicular fluid of women with endometriosis and influences mouse and human embryonic potential

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Xiong Li ◽  
Wenbi Zhang ◽  
Jing Fu ◽  
Yan Xu ◽  
Ruihuan Gu ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Polymerase Chain Reaction Analysis ◽  
Fertilization Rate ◽  
Wnt Signalling ◽  
Signalling Pathway ◽  
University Hospital ◽  
Vitro Fertilization ◽  
Human Oocytes ◽  
Wnt Signalling Pathway

Abstract Background Previous work demonstrated that there are numerous miRNAs in human follicular fluids, some of which are associated with reproductive diseases. In the current study, we sought to determine whether microRNAs (miRNAs) in the follicular fluid (FF) are differentially expressed between women with and without endometriosis and to uncover the association of miRNAs with the oocyte and embryonic development potential. Methods FF was harvested from 30 women with endometriosis and 30 women without who underwent in vitro fertilization treatment at the University Hospital between February and December 2016. The FF samples were subjected to miRNA profiling and validation via quantitative reverse transcription polymerase chain reaction analysis. Mouse/human metaphase-I (MI) oocytes were harvested and micro-injected with an miR-451 inhibitor, and the effects of miR-451 knockdown on Wnt/WNT signalling genes were investigated. Results Oocyte number, fertilization rate, and number of available embryos were decreased significantly in women with endometriosis relative to those without endometriosis. Hsa-miR-451 in FF was downregulated in endometriosis patients relative to control subjects (P < 0.01). Moreover, the proportions of mouse/human MI oocytes that developed into 2-pronuclei (2PN), 2-cell, 8–10-cell and blastocyst-stage embryos were affected by miR-451 knockdown in mouse/human oocytes. Components of the Wnt signalling pathway were aberrantly expressed in the mouse/human oocytes and embryos in the miR-451 inhibitor-injected group. Conclusions miR-451 was downregulated in FF samples from endometriosis patients and was modestly effective in distinguishing endometriosis patients from non-endometriosis patients. miR-451 downregulation in mouse and human oocytes affected pre-implantation embryogenesis by suppressing the Wnt signalling pathway. This miRNA might serve as a novel biomarker of oocyte and embryo quality in assisted reproductive treatment.

scholarly journals In vitro maturation of buffalo oocytes and fertilization by cattle spermatozoa

2012 ◽  
Vol 41 (1) ◽  
pp. 6-12
Author(s):  
MAMY Khandoker ◽  
MMT Reza ◽  
LY Asad ◽  
S Saha ◽  
AS Apu ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Follicular Fluid ◽  
Bovine Serum ◽  
In Vitro Maturation ◽  
Fertilization Rate ◽  
Maturation Rate ◽  
Normal Fertilization

The present research was undertaken to explore the maturation of buffalo oocytes using bovine follicular fluid (BFF) and bovine serum albumin (BSA), as well as subsequent fertilization using cattle spermatozoa. The cumulus oocyte complexes (COCs) were collected by aspiration of slaughterhouse buffalo ovaries. Maturation was performed in TCM 199 supplemented with 10% BFF, 5% BSA or without supplementation (control). The COCs were fertilized in Brackett and Oliphant (BO) medium using capacitated fresh cattle spermatozoa. It was observed that the percentage of COCs reached to M-II stages were 40.78±3.84, 65.74±2.39 and 67.52±0.85; normal fertilization (formation of 2 pronuclei) were 23.28±3.00, 29.30±0.73 and 30.52±1.21 for control, 10% BFF and 5% BSA supplementation, respectively. The supplementation of BFF (10%) and BSA (5%) were given similar results on maturation and increased significantly (p<0.05) than that of the control. It was observed that cattle spermatozoa were fertilized by the buffalo oocytes and the fertilization rate was 23.28% to 30.52% in BFA and BSA supplemented media, respectively. It can be concluded that buffalo oocytes might be fertilized using capacitated cattle spermatozoa and both 10% BFF and 5% BSA could be supplemented in maturation media to enhance the maturation rate as well as fertilization of buffalo oocytes.http://dx.doi.org/10.3329/bjas.v41i1.11969

scholarly journals Association between Follicular Fluid Leptin and Serum Insulin Levels in Nonoverweight Women with Polycystic Ovary Syndrome

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
G. Garruti ◽  
R. de Palo ◽  
M. T. Rotelli ◽  
S. Nocera ◽  
I. Totaro ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Polycystic Ovary ◽  
Serum Insulin ◽  
Fertilization Rate ◽  
Serum Leptin ◽  
Vitro Fertilization ◽  
Insulin Levels ◽  
Fertilization Rates ◽  
And Control

Aims. We evaluated the links between leptin and visfatin levels and fertilization rates in nonoverweight (NOW) women with PCOS (NOW-PCOS) from Apulia undergoing in vitro fertilization/embryo transfer (IVF).Materials and Methodology. We recruited 16 NOW women with PCOS (NOW-PCOS) and 10 normally ovulating NOW women (control-NOW). All women underwent IVF. Androgens, 17-β-estradiol (17β-E2), and insulin levels were measured in plasma and/or serum and leptin and visfatin levels were assayed in both serum and follicular fluid (FF-leptin, FF-visfatin).Results. In NOW-PCOS, both serum and FF-leptin were significantly lower than in control-NOW. In NOW-PCOS, significant correlations were found between BMI and serum leptin and insulinemia and FF-leptin. By contrast, in control-NOW, FF-leptin levels were not correlated with insulinemia. Serum visfatin levels were not significantly different in NOW-PCOS and control-NOW, but FF-visfatin levels were 1.6-fold higher, although not significantly, in NOW-PCOS than in control-NOW.Conclusions. Both serum leptin levels and FF-leptin are BMI- and insulin-related in Southern Italian NOW-PCOS from Apulia. In line with other reports showing that FF-leptin levels are predictive of fertilization rates, lower than normal FF-leptin levels in NOW-PCOS may explain their lower fertilization rate and this may be related to the level of insulin and/or insulin resistance.

scholarly journals Oxidative stress and DNA damage status in couples undergoing in vitro fertilization treatment

2021 ◽  
Author(s):  
Iman Al-Saleh ◽  
Serdar Coskun ◽  
Reem Al-Rouqi ◽  
Tahreer Al-Rajudi ◽  
Chafica Eltabache ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
In Vitro Fertilization ◽  
Seminal Plasma ◽  
Follicular Fluid ◽  
Fertilization Rate ◽  
Reproductive Hormones ◽  
Vitro Fertilization ◽  
Poor Fertilization

This study examined the status of oxidative stress in 599 couples undertaking in vitro fertilization (IVF) treatment and its association with reproductive hormones, smoking, and outcomes. Oxidative stress biomarkers such as malondialdehyde (MDA), 8-hydroxy-2-deoxyguanosine (8-OHdG), hydrogen peroxide (H2O2), catalase (CAT), and total antioxidant capacity (TAC) were determined in follicular fluid and seminal plasma. Tail moment (TM) was used to evaluate DNA damage in sperm and granulosa cells. Reproductive hormones in serum and cotinine (COT) in urine, follicular fluid, and seminal plasma samples were determined. We used log-binomial multivariate regression to estimate relative risks for the association between oxidative stress/DNA damage and IVF binary outcomes (fertilization rate, biochemical pregnancy, clinical pregnancy, and live birth). We observed an increase in the oxidative stress markers MDA, 8-OHdG, and H2O2 in follicular fluid and seminal plasma, but a decrease in the antioxidant protection markers CAT and TAC. The MDA, 8-OHdG, and H2O2 levels were significantly higher in seminal plasma than in follicular fluid, while TAC, CAT, and TM were higher in follicular fluid (p < 0.001). Although women were nonsmokers, COT levels >50 µg/l were observed in 5.7% (urine) and 1.4% (follicular fluid). An increase in the CAT levels of follicular fluid was associated with a 48 and 41% decrease in the risk of poor fertilization rate (≤50%) and unsuccessful live birth, respectively. After the models were adjusted for hormonal factors, the associations remained the same, except that elevated TAC in follicular fluid became significantly associated with a decrease of 42% in the risk of poor fertilization rate (≤50%).

scholarly journals Steroid Hormones of Follicular Fluid and the Outcome of in Vitro Fertilization

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Aleksandra Gavrilovic ◽  
Jelena Cekovic ◽  
Aida Parandilovic ◽  
Aleksandar Nikolov ◽  
Predrag Sazdanovic ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Steroid Hormones ◽  
Follicular Fluid ◽  
Fertilization Rate ◽  
Vitro Fertilization ◽  
Gynecology And Obstetrics ◽  
Clinical Center ◽  
Significant Difference ◽  
Assisted Fertilization

Abstract One of the succes factors of biomedically assisted fertilization is the regular maturation of one or more oocytes. The quality of the oocytes is significantly influenced by the environment in which it is located, the so-called „microenvironment” that includes cumulus cells, follicular fluid in which hormones and growth factors involved in its growth and development are secreted. The main aim was to examine whether the concentration of steroid hormones in the follicular fluid affects the rate of fertilization and the outcome of the in vitro fertilization process itself. The study included 31 patients who were included in vitro fertilization procedure at the Department for Biomedically Assisted Fertilization, Clinic for Gynecology and Obstetrics, Clinical Center Kragujevac. We used follicular fluid as biological material for analysis. Examination of the obtained follicular fluid and collection of oocytes under a stereomicroscope was done in the embryological laboratory at the Department. Biochemical parameters of follicular fluid were analyzed in the Department for Laboratory Diagnostics, Clinical Center Kragujevac. In vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) methods were used as the fertilization method. The criteria of the Istanbul Consensus of Clinical Embryologists were used as a reference framework for embryo quality assessment. Pregnancy was confirmed by a positive serum level of the hormone β-hCG 14 days after embryo transfer. A software package ЅРЅЅ 20 was used for statistical data processing. The results of the analysis of follicular fluid samples show that there was no statistically significant difference in the concentration of estradiol, progesterone and testosterone in follicular fluid in relation to fertilization rate and the outcome of in vitro fertilization. Based on our results, it can be concluded that the concentration of steroid hormones did not affect fertilization rate and the outcome of in vitro fertilization.

206 THE IMPORTANCE OF CUMULUS EXPANSION FOR MONOSPERMIC FERTILIZATION OF PORCINE CUMULUS - OOCYTE COMPLEXES

2011 ◽  
Vol 23 (1) ◽  
pp. 202
Author(s):  
J. Beek ◽  
J. Bijttebier ◽  
D. Maes ◽  
H. Nauwynck ◽  
A. Van Soom
Keyword(s):  
Follicular Fluid ◽  
In Vitro Maturation ◽  
Indirect Evidence ◽  
Fertilization Rate ◽  
Control Group ◽  
Cumulus Expansion ◽  
Mean Diameter ◽  
Sperm Penetration ◽  
Epidermal Growth

In pigs, the exact role of the cumulus oöphurus during IVF still needs to be clarified. Indirect evidence exists that the rate of cumulus expansion is positively correlated with the defense against polyspermy. Epidermal growth factor (EGF) acts synergistically with FSH in the synthesis of hyaluronic acid, the deposition of which in the extracellular matrix is a prerequisite for cumulus expansion. Therefore, we aimed to evaluate the importance of cumulus expansion for fertilization results of porcine cumulus–oocyte complexes (COC) by using different EGF concentrations in the maturation medium. Cumulus–oocyte complexes were matured in vitro in NCSU23 medium supplemented with 10% follicular fluid (FF, obtained from 6- to 10-mm follicles) or 10% serum and 10, 20, or 50 ng mL–1 of EGF (n = 480 per experiment). In vitro maturation (IVM) in the presence of 10% FF and 10 ng mL–1 of EGF served as the control group. At 0, 22, 36, and 44 h of IVM, 20 COC of each group were selected for evaluation of cumulus expansion by measuring the maximum distance across the cumulus matrix (3 replications). Matured COC were co-incubated with frozen–thawed semen (6000 spermatozoa per oocyte) for 6 h. Subsequently, oocytes were cultured for 18 h. Zygotes were stained with 10 μg mL–1 of bis-benzimide (Hoechst) to assess the fertilization rate, polyspermy, and sperm penetration index (sp index, mean number of penetrated spermatozoa per fertilized oocyte; 2 replications). Differences in cumulus diameter were analyzed by one-way ANOVA. Fertilization parameters were analyzed by applying a logistic regression model to the results. Cumulus–oocyte complexes selected for IVM had a mean diameter of 240 μm. After 22 h of IVM in 10% FF, the mean diameter of COC was 336, 313, and 300 μm for 10, 20, and 50 ng mL–1 of EGF, respectively. After 44 h of IVM, these diameters had increased to 425, 388, and 397 μm. Twenty-two hours of IVM in 10% serum resulted in a COC diameter of 296, 305, and 276 μm for 10, 20, and 50 ng mL–1 of EGF. After 44 h of IVM, these diameters reached 330, 325, and 275 μm, respectively. Only 10% serum with 50 ng mL–1 of EGF proved unfavorable for cumulus expansion (P < 0.05). In vitro maturation for 44 h in 10% serum resulted in a smaller rate of cumulus expansion compared with IVM in 10% FF (P < 0.05), irrespective of EGF concentration. Penetration rate fluctuated between 84 and 100%, with no significant differences. Monospermic fertilization was lower in COC matured in the presence of 50 ng mL–1 of EGF compared with the control group (P < 0.05). The sp index increased in parallel with EGF concentrations and was higher after IVM in 10% serum than in 10% FF. Oocytes were penetrated by 3.1, 4.3, and 6.0 spermatozoa after IVM in serum with 10, 20, and 50 ng mL–1 of EGF, respectively. Results showed a tendency toward a lower rate of cumulus expansion concomitant with higher EGF concentrations. Follicular fluid was superior to serum in supporting cumulus expansion. Oocytes were penetrated by more spermatozoa when matured in 10% serum and 20 or 50 ng mL–1 of EGF compared with 10 ng mL–1 of EGF. Thus, the degree of cumulus expansion appears to be related to the sp index, confirming that the cumulus matrix may play a role in the polyspermy defense. This study was supported by Research Foundation-Flanders.

scholarly journals Expression of microRNA in follicular fluid in women with and without PCOS

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Alexandra E. Butler ◽  
Vimal Ramachandran ◽  
Shahina Hayat ◽  
Soha R. Dargham ◽  
Thomas Keith Cunningham ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Follicular Fluid ◽  
Quantitative Polymerase Chain Reaction ◽  
Oocyte Retrieval ◽  
Fertilization Rate ◽  
Data Set ◽  
Free Androgen Index ◽  
Fertilisation Rate ◽  
Normal Women

Abstract Several studies have shown the expression of small non-coding microRNA (miRNA) changes in PCOS and their expression in follicular fluid has been described, though the number of studies remains small. In this prospective cohort study, miRNA were measured using quantitative polymerase chain reaction (qPCR) in 29 weight and aged matched anovulatory women with PCOS and 30 women without from follicular fluid taken at the time of oocyte retrieval who were undergoing in vitro fertilization (IVF); miRNA levels were determined from a miRNA data set. 176 miRNA were detected, of which 29 differed significantly between normal women and PCOS women. Of these, the top 7 (p < 0.015) were miR-381-3p, miR-199b-5p, miR-93-3p, miR-361-3p, miR-127-3p, miR-382-5p, miR-425-3p. In PCOS, miR-382-5p correlated with age and free androgen index (FAI), miR-199b-5p correlated with anti-mullerian hormone (AMH) and miR-93-3p correlated with C-reactive protein (CRP). In normal controls, miR-127-3p, miR-382-5p and miR-425-3p correlated with the fertilisation rate; miR-127-3p correlated with insulin resistance and miR-381-3p correlated with FAI. Ingenuity pathway assessment revealed that 12 of the significantly altered miRNA related to reproductive pathways, 12 miRNA related to the inflammatory disease pathway and 6 were implicated in benign pelvic disease. MiRNAs differed in the follicular fluid between PCOS and normal control women, correlating with age, FAI, inflammation and AMH in PCOS, and with BMI, fertilization rate (3 miRNA), insulin resistance, FAI and inflammation in control women, according to Ingenuity Pathway Analysis.

Follicular fluid and assisted reproductive technology programs outcomes (literature review)

GYNECOLOGY ◽  
2020 ◽  
Vol 21 (6) ◽  
pp. 36-40
Author(s):  
Anna G. Burduli ◽  
Natalia A. Kitsilovskaya ◽  
Yuliya V. Sukhova ◽  
Irina A. Vedikhina ◽  
Tatiana Y. Ivanets ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Assisted Reproductive Technology ◽  
Follicular Fluid ◽  
Economic Cost ◽  
Reproductive Technology ◽  
Art Programs ◽  
Vitro Fertilization ◽  
Non Invasive ◽  
Established Fact

The review presents data on metabolites in the follicular fluid (FF) from the perspective of reproductive medicine and their use in order to predict outcomes of assisted reproductive technology (ART) programs. It considers various components of this biological medium (hormones, lipids, melatonin, etc.) with an assessment of their predictive value in prognosis of the effectiveness of in vitro fertilization (IVF) programs. The data on experimental directions in this field and the prospects for their use in clinical practice are presented. The article emphasizes that the growing clinical need and the unsolved problem of increasing the effectiveness of ART programs determine the need for further studies of the FF composition. Materials and methods. The review includes data related to this topic from foreign and Russian articles found in PubMed which were published in recent years. Results. Given the established fact of a direct effect of FF composition on growth and maturation of oocytes, and further, on the fertilization process, various FF metabolites are actively investigated as non-invasive markers of quality of oocytes/embryos. The article provides data on the experimental directions in this field and the prospects for their use in clinical practice. However, clinical studies of a relation between various FF metabolites levels and outcomes of IVF programs are contradictory. Conclusion. Owing large economic cost for treatment of infertility with IVF, there is need for expansion and intensification of studies to identify and use reliable predictors in prognosis of ART programs outcomes.

scholarly journals Priming with a gonadotropin-releasing hormone agonist before immature oocyte retrieval may improve maturity of oocytes and outcome in in vitro maturation (IVM) cycle: a case report

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
A. Smirnova ◽  
M. Anshina ◽  
E. Shalom Paz ◽  
A. Ellenbogen
Keyword(s):  
Fertility Preservation ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Oocyte Retrieval ◽  
Fertilization Rate ◽  
Gonadotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Nuclear Maturation ◽  
Gonadotropin Releasing Hormone Agonist

Abstract Background The concept of using a gonadotropin-releasing hormone agonist (GnRH-a) instead of human chorionic gonadotropin for triggering ovulation in patients treated with an antagonist protocol for in vitro fertilization (IVF) has become a routine clinical practice. It may promote oocyte nuclear maturation, resumption of meiosis and cumulus expansion. It seems that this attempt could be beneficial in an in vitro maturation (IVM) oocyte cycle performed for polycystic ovarian syndrome as well as for other indications such as urgent fertility preservation in patients with malignancies or unusual indications. Case presentation We present the case of a Caucasian patient who needed fertility preservation when routine natural IVF treatment did not yield oocyte retrieval, followed by three IVM cycles, priming ovulation with a GnRH-a. In total, 12 oocytes were obtained, all matured 4.5 hours after incubation in maturation media. The fertilization rate after intracytoplasmic sperm injection was 83%. Six good-quality embryos were vitrified. Conclusions It seems that triggering with a GnRH-a in selected cases may replace human chorionic gonadotropin in IVM of oocytes and could be highly beneficial in terms of obtaining high-grade embryos and possible pregnancy.

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