Selection of optimum composition and study of specific activity of the pediatric medication form of dimedrol

The enzyme rhodanese (EC 2.8.1.1) is an ubiquitous enzyme which is present in all living organisms, from bacteria to man. It is speculated that this enzyme plays a central role in cyanide detoxification. However, its wide tissue distribution suggests this enzyme might perform other functions beside cyanide detoxification. Although the distribution of rhodanese in different tissues of human and domestic animals has been studied, little is known about the pattern of distribution and physiological roles of this enzyme in the cat. The purpose of this investigation was to determine the enzyme levels and compare the distribution of this enzyme in different tissues of the cat. A selection of tissue samples was assayed for rhodanese activity. The protein content of tissue extracts and enzymatic activities were calculated as units per gram tissue and units per milligram protein of the tissue. Results showed that in terms of units per milligram protein of the tissue (specific activity of the enzyme), colon and rectum mucosal layers and testis were the richest sources of the enzyme followed by ovary, mucosal layer of jejunum and liver. With respect to units/gram tissue, liver followed by testis, colon and rectum mucosal layers, ovary and mucosa of jejunum exhibited highest activities. The results of this study will allow one to speculate on the involvement of rhodanese in several biochemical and physiological functions in different tissues and organs of this species.

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Application of miniaturized disk bend test technique for selection of optimum composition of candidate materials for fusion reactors

Journal of Nuclear Materials ◽

10.1016/0022-3115(92)90536-t ◽

1992 ◽

Vol 189 (2) ◽

pp. 222-225 ◽

Cited By ~ 3

Author(s):

A.B. Tsepelev ◽

I.L. Poymenov

Keyword(s):

Bend Test ◽

Optimum Composition ◽

Fusion Reactors ◽

Test Technique ◽

Selection Of

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RNA SYNTHESIS IN CHINESE HAMSTER CELLS

The Journal of Cell Biology ◽

10.1083/jcb.36.3.583 ◽

1968 ◽

Vol 36 (3) ◽

pp. 583-593 ◽

Cited By ~ 36

Author(s):

M. D. Enger ◽

R. A. Tobey ◽

A. G. Saponara

Keyword(s):

Ribosomal Rna ◽

Rna Synthesis ◽

Specific Activity ◽

Chinese Hamster Ovary Cells ◽

Chinese Hamster ◽

Sedimentation Analysis ◽

Ovary Cells ◽

Chinese Hamster Cells ◽

Mechanical Selection ◽

Selection Of

The incorporation of methionine-methyl-14C into 18S ribosomal RNA of cultured Chinese hamster ovary cells in early and late interphase has been determined by zone-sedimentation analysis of phenol-extracted RNA preparations. Synchronized cell cultures were prepared for these studies by thymidine treatment and by mechanical selection of mitotic cells. The specific activity of 18S RNA labeled in late interphase was found to be 1.1–1.2 times that of 18S RNA labeled in early interphase. Upon correction for increase in RNA mass, the rate of methylation of 18S RNA in late interphase is about 1.9 times that in early interphase.

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Bio-Engineered Nisin with Increased Anti-Staphylococcus and Selectively Reduced Anti-Lactococcus Activity for Treatment of Bovine Mastitis

International Journal of Molecular Sciences ◽

10.3390/ijms22073480 ◽

2021 ◽

Vol 22 (7) ◽

pp. 3480

Author(s):

Des Field ◽

Kiera Considine ◽

Paula M. O’Connor ◽

R. Paul Ross ◽

Colin Hill ◽

...

Keyword(s):

Specific Activity ◽

Negative Impact ◽

Bovine Mastitis ◽

Active Agent ◽

Antibiotic Use ◽

Human Medicine ◽

Target Spectrum ◽

Animal Pathogens ◽

Reduced Activity ◽

Selection Of

Bovine mastitis is a significant economic burden for dairy enterprises, responsible for premature culling, prophylactic and therapeutic antibiotic use, reduced milk production and the withholding (and thus wastage) of milk. There is a desire to identify novel antimicrobials that are expressly directed to veterinary applications, do not require a lengthy milk withholding period and that will not have a negative impact on the growth of lactic acid bacteria involved in downstream dairy fermentations. Nisin is the prototypical lantibiotic, a family of highly modified antimicrobial peptides that exhibit potent antimicrobial activity against many Gram-positive microbes, including human and animal pathogens including species of Staphylococcus and Streptococcus. Although not yet utilized in the area of human medicine, nisin is currently applied as the active agent in products designed to prevent bovine mastitis. Over the last decade, we have harnessed bioengineering strategies to boost the specific activity and target spectrum of nisin against several problematic microorganisms. Here, we screen a large bank of engineered nisin derivatives to identify novel derivatives that exhibit improved specific activity against a selection of staphylococci, including mastitis-associated strains, but have unchanged or reduced activity against dairy lactococci. Three such peptides were identified; nisin A M17Q, nisin A T2L and nisin A HTK.

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Selection of the optimum composition of an alumina support of Pd/Al2O3 catalysts for pyrolysis gasoline hydrogenation

Catalysis in Industry ◽

10.1134/s207005041004015x ◽

2010 ◽

Vol 2 (4) ◽

pp. 393-401

Author(s):

A. A. Lamberov ◽

I. R. Il’yasov ◽

I. F. Khalilov ◽

A. Sh. Bikmurzin ◽

V. M. Shatilov ◽

...

Keyword(s):

Optimum Composition ◽

Alumina Support ◽

Pyrolysis Gasoline ◽

Selection Of

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Qualitative Indicators of Experimental Brucellosis Antigen Preparations Designed for Cellular Tests in vitro

Problems of Particularly Dangerous Infections ◽

10.21055/0370-1069-2020-3-83-88 ◽

2020 ◽

pp. 83-88

Author(s):

S. A. Kurcheva ◽

D. A. Kovalev ◽

D. G. Ponomarenko ◽

Yu. V. Siritsa ◽

M. V. Kostyuchenko ◽

...

Keyword(s):

Specific Activity ◽

Laboratory Diagnosis ◽

Polysaccharide Composition ◽

Methodological Approaches ◽

Cellular Tests ◽

Refractometric Detection ◽

Experimental Preparation ◽

Selection Of

In order to develop the most diagnostically informative methods for carrying out antigen-stimulated cellular tests in vitro a careful selection of stimulating agent (antigen) is required, possessing an adequate activating potential and providing specificity of the reaction.Objective of the study was to identify the qualitative indicators of experimental batches of brucellosis antigen preparations designed for cellular tests in vitro.Materials and methods. Initially we produced antigen complexes of brucellosis microbe on the basis of the vaccine strains of three epidemically significant Brucella species (B. abortus, B. melitensis, B. suis). Quantitative determination of WsAg and PPBC proteins of experimental preparation series was performed applying capillary electrophoresis. Qualitative composition was assessed through ion exchange liquid chromatography with refractometric detection.Results and discussion. We have specified physical-chemical features, investigated chromatographic profiles and composition of protein fractions, as well as tried the produced experimental batches of brucellosis antigen preparations. After analyzing the defined protein and polysaccharide composition of the obtained WsAg samples, one can conclude that WsAg preparation cannot be used for cellular tests as the probability of non-specific lymphocyte reaction manifestation in vitro was experimentally proven. By contrast, complex brucellosis antigen preparation PPBC has an expressed specific activity and specificity under in vitro conditions and the prospects to be used when developing methodological approaches for laboratory diagnosis of brucellosis and assessment of de facto immunity rate in risk contingents after vaccination. The obtained parameters will allow for proper quality provision when manufacturing the developed experimental PPBC preparation designed for cellular tests in vitro, taking into account modern validation and standardization regulations.

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PHOTOSYNTHESIS AND RUBISCO IN STRAWBERRY SPECIES

HortScience ◽

10.21273/hortsci.25.9.1166c ◽

1990 ◽

Vol 25 (9) ◽

pp. 1166c-1166

Author(s):

John W. Moon

Keyword(s):

Cell Surface ◽

Surface Area ◽

Soluble Protein ◽

Specific Activity ◽

Mesophyll Cell ◽

Leaf Nitrogen ◽

Leaf Thickness ◽

Carboxylation Efficiency ◽

Cell Surface Area ◽

Selection Of

CO2 assimilation (20-25%) and carboxylation efficiency (30-35%) was greater (P < 0.01) in Fragaria chiloensis, clone RCP37 than F. ananassa cv. `Midway'. Both leaf thickness (P < 0.05) and internal mesophyll cell surface area (P < 0.01) was greater in RCP37 than `Midway'. No differences in Rubisco specific activity were observed between the 2 species, whereas Rubisco amount was 40% greater (P < 0.01) and leaf nitrogen per area was 30-35% greater in RCP37 compared to `Midway'. Potential for selection of clones with high photosynthesis by screening for soluble protein will be discussed.

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The need for individual selection of probiotics containing lactobacilli and enterococci to increase the effectiveness of therapy for campylobacteriosis

Experimental and Clinical Gastroenterology ◽

10.31146/1682-8658-ecg-186-2-88-93 ◽

2021 ◽

pp. 88-93

Author(s):

K. D. Ermolenko ◽

N. P. Boldyreva ◽

E. A. Martens ◽

L. I. Zhelezova ◽

S. V. Sidorenko ◽

...

Keyword(s):

Antimicrobial Agents ◽

Specific Activity ◽

High Sensitivity ◽

Saccharomyces Boulardii ◽

Antagonistic Activity ◽

Individual Selection ◽

Treatment Regimens ◽

Campylobacter Spp ◽

Selection Of

The article highlights the problem of improving the rational treatment of campylobacteriosis. Probiotics are present in treatment regimens along with antibiotics, which have the advantage that they do not violate intestinal microbiocenosis and provide the ability to correct dysbiotic conditions. As well as antimicrobial agents, probiotics have different effects on the growth of pathogenic microorganisms. Campylobacter spp. probiotics in the in vitro system. The article studies the anticampylobacter activity of probiotic cultures of Enterococcus faecium L3, Lactobacillus plantarum 8 R-A3, a mixture of Lactobacillus acidophilus and Saccharomyces boulardii by two-layer agar and droplet method. Analysis of the antagonistic activity of chemically synthesized bacteriocins. The high sensitivity of Campylobacter spp was presented. to probiotics having lactobacilli and enterococci, as well as their metabolites (including bacteriocins). The strain-specific activity of probiotics and its dependence on their ability to produce bacteriocins were found. The results and data of other researchers indicate the need for individual selection of probiotics for the treatment of campylobacteriosis, the feasibility of analyzing the bacteriocinogenicity of the strains and testing their effect on the growth of clinical isolates.

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Selection of the Optimal Medium for Adsorption of Plant Proteases

Chemistry Proceedings ◽

10.3390/eccs2020-07525 ◽

2020 ◽

Vol 2 (1) ◽

pp. 3

Author(s):

Victoria Koroleva ◽

Svetlana Olshannikova ◽

Marina Holyavka ◽

Alexander Pashkov ◽

Valeriy Artyukhov

Keyword(s):

Specific Activity ◽

Immobilized Enzymes ◽

Global Market ◽

Adsorption Method ◽

Native Form ◽

Glycine Buffer ◽

Chitosan Matrix ◽

Plant Proteases ◽

Optimal Medium ◽

Selection Of

Immobilized enzymes are the most sought-after preparations in the global market. They are used in medicine, veterinary medicine, the food industry, winemaking and brewing. The simplest method for immobilizing biocatalysts on insoluble carriers is the simple adsorption method. Its advantage is that it preserves the natural conformation of the enzyme, which slightly reduces its catalytic ability compared to the native form. In our study, we carried out the selection of optimal conditions for adsorption immobilization of acid-soluble chitosan (Mr = 350 kDa) enzymes of plant origin (ficin, papain and bromelain) on a matrix. Ficin (EC 3.4.22.3), papain (EC 3.4.22.2) and bromelain (EC 3.4.22.4) (Sigma) were chosen as the objects of study, azocasein (Sigma) was used as a substrate for hydrolysis and an acid-soluble high-molecular-weight chitosan (350 kDa) was used as an immobilization matrix, synthesized by Bioprogress CJSC. Suitable buffer systems for immobilization were identified by the optimal ratio of protein content and total and specific activity. Ficin is immobilized on a chitosan matrix using glycine buffer with a pH of 8.6. Glycine buffer with a pH of 8.6–10.5 is an optimal medium for sorption of papain on chitosan. Bromelain is immobilized on a chitosan matrix under Tris-glycine buffer with pH 8.5 conditions.

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