Characterization of a newly established feline lymphoma-derived cell line (BKD) lacking T and B cell surface markers

1986 ◽  
Vol 22 (5) ◽  
pp. 273-279 ◽  
Author(s):  
Robert W. Engelman ◽  
Katsuhiko Machida ◽  
Ross E. Longley ◽  
Wing T. Liu ◽  
Liem Q. Trang ◽  
...  
Keyword(s):  
Cell Surface ◽  
B Cell ◽  
Cell Line ◽  
Surface Markers ◽  
Cell Surface Markers

scholarly journals Establishment and Expression of Cytokines in a Theileria annulata-Infected Bovine B Cell Line

Genes ◽  
2019 ◽  
Vol 10 (5) ◽  
pp. 329 ◽  
Author(s):  
Rashid ◽  
Guan ◽  
Luo ◽  
Zhao ◽  
Wang ◽  
...  
Keyword(s):  
B Cells ◽  
Cell Surface ◽  
B Cell ◽  
Cell Line ◽  
Cytokine Production ◽  
Theileria Annulata ◽  
Transformed Cells ◽  
Specific Cell ◽  
Surface Markers ◽  
Cell Surface Markers

This study aimed to establish a pure single-cell Theileria annulata-infected B cell line for the assessment of cytokine production in transformed and lipopolysaccharide (LPS)-stimulated cells. Several studies have aimed to identify cell surface markers in T. annulata-transformed cells; however, no information on cytokine production in these cells is available. To investigate the potential of the transformed cells to produce cytokines and their potential responses to antigen-stimulation, we purified mature B cells (CD21) from the whole blood of cattle experimentally infected with the T. annulata Kashi strain by magnetic separation. The purity and specificity of the established cell line was assessed by the identification of specific cell surface markers (CD21, IgM, and WC4) by flow cytometry analysis. The transcript levels of the cytokines IL1A, IL1B, IL2, IL4, IL6, IL8, IL10, IL16, LTA, TGFB1, TNFA, IFNA, and IFNB in transformed, buparvaquone (BW720c)-treated cells, and antigen-stimulated cells were analyzed by quantitative polymerase chain reaction (qPCR) using cDNA from these cells. A T. annulata-infected bovine B cell line was successfully established with a purity of ~98.8% (CD21). IL4 and IL12A were significantly (p < 0.01) upregulated in the transformed cells. In BW720c-treated transformed cells, IL12B, TGFB1, and IFNB were significantly (p < 0.01) upregulated. Notably, no significant (p > 0.05) upregulation of cytokines was observed in LPS-stimulated transformed cells. Moreover, IL1A, IL1B, IL8, and IL16 were significantly (p < 0.01) upregulated in LPS-stimulated B cells. Our data signify the potential use of this cell line for cytokine production, observance of immunoglobulins, and production of an attenuated vaccine against tropical theileriosis.

Trogocytosis of multiple B-cell surface markers by CD22 targeting with epratuzumab

Blood ◽  
2013 ◽  
Vol 122 (17) ◽  
pp. 3020-3029 ◽  
Author(s):  
Edmund A. Rossi ◽  
David M. Goldenberg ◽  
Rosana Michel ◽  
Diane L. Rossi ◽  
Daniel J. Wallace ◽  
...  
Keyword(s):  
B Cells ◽  
Autoimmune Disease ◽  
Cell Surface ◽  
B Cell ◽  
Regulatory Proteins ◽  
Surface Markers ◽  
B Cell Antigen Receptor ◽  
Cell Surface Markers ◽  
Effector Cells ◽  
Key Points

Key Points Epratuzumab induces the reduction of multiple B-cell antigen receptor–modulating proteins on the surface of B cells via their trogocytosis to effector cells. Modulation of B cells by trogocytosis of key regulatory proteins may be an important mechanism of immunotherapy of autoimmune disease.

scholarly journals Phenotype Characterization of Human Adipose-Derived Stem Cell by Flow Cytometry

2021 ◽  
Author(s):  
Celeste Limoli ◽  
Paolo Giuseppe Limoli ◽  
Marcella Nebbioso
Keyword(s):  
Adipose Tissue ◽  
Cell Surface ◽  
Stromal Vascular Fraction ◽  
Surface Markers ◽  
Adipose Derived Stem Cells ◽  
Cell Surface Markers ◽  
Cell Surface Antigens ◽  
Adipose Derived Stem Cell

Background: Developing an efficient and standardized method to isolate and characterize adipose-derived stem cells (ASCs) from the stromal vascular fraction (SVF) of the adipose tissue for clinical application represents one of the major challenges in cell therapy and tissue engineering. Methods: In this study, we proposed an innovative, non-enzymatic protocol to collect clinically useful ASCs within freshly isolated SVF from adipose tissue by centrifugation of the infranatant portion of lipoaspirate and to determine the characteristic cytofluorimetric pattern, prior to in vitro culture. Results: The SVF yielded a mean of 73,32 \pm\ 10,89% cell viability evaluated with CALCEINA-FITC, i.e. cell-permeant dye. The ASCs were positive for PC7-labeled mAb anti-CD34 and negative for both PE-labeled mAb anti-CD31 and APC-labeled mAb anti-CD45. The frequency of ASCs estimated according to the panel of cell surface markers used was 51,06%\ \pm 5,26% versus the unstained ASCs subpopulation that was 0,74%\pm0,84% (P&lt;0.0001). The ASCs events/\muL were 1602,13/\muL \pm 731,87/\muL. Conclusion: Our findings suggested that ASCs found in freshly isolated adipose SVF obtained by centrifugation of lipoaspirate can be immunophenotypically identified with a basic panel of cell surface markers. These findings aimed to provide standardization and contribute to reducing the inconsistency on reported cell surface antigens of ASC derived from the existing literature.

Flow cytometry and live cell imaging for characterization of cell surface markers on human tissue-derived progenitors

Cytotherapy ◽  
2020 ◽  
Vol 22 (5) ◽  
pp. S69-S70
Author(s):  
W.A. Bova ◽  
V.R. Mantripragada ◽  
V. Luangphakdy ◽  
G.F. Muschler
Keyword(s):  
Flow Cytometry ◽  
Cell Surface ◽  
Human Tissue ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Surface Markers ◽  
Cell Surface Markers

Expression of v-rel induces mature B-cell lines that reflect the diversity of avian immunoglobulin heavy- and light-chain rearrangements

1988 ◽  
Vol 8 (12) ◽  
pp. 5358-5368
Author(s):  
C F Barth ◽  
E H Humphries
Keyword(s):  
Cell Surface ◽  
B Cell ◽  
Cell Lines ◽  
Light Chain ◽  
Lymphoid Cells ◽  
Surface Markers ◽  
Cell Surface Markers ◽  
Chain Locus ◽  
Light Chain Locus

The infection of newly hatched chickens with reticuloendotheliosis virus strain T (REV-T) and a nonimmunosuppressive helper virus, chicken syncytial virus, induces rapidly metastatic B-cell lymphomas. In vivo analysis of these tumors with monoclonal antibodies detected the expression of the B-cell surface markers immunoglobulin M (IgM), CIa, Bu2, and CLA-1, but not IgG, Bu1, or a T-cell surface marker, CT-1. Cell lines derived from tumors exhibited the same pattern of staining, suggesting that expression of cell surface markers does not change during in vitro cell line development. All cell lines examined synthesized IgM in varying amounts. Northern (RNA blot) analysis confirmed abundant expression of v-rel mRNA, and Southern analysis revealed rearrangement of both heavy- and light-chain immunoglobulin loci. Analysis of the light-chain locus demonstrated that 20 of 22 lines contained a single rearranged allele. With respect to specific restriction enzyme sites within the V lambda 1 gene, the active allele in any given clone was either diversified or nondiversified. In contrast, examination of the heavy-chain loci within these lines demonstrated that 16 of the 22 had both alleles rearranged. Further diversification of the V lambda 1 locus did not occur after prolonged in vitro passage of the cell lines. We propose that v-rel expression arrests diversification of the light-chain locus in these lymphoid cells, allowing the production of stable, clonal B-cell populations. The development of these and similar cell lines will make it possible to identify specific stages of avian lymphoid ontogeny and to study the mechanism of rearrangement and diversification in the avian B lymphocyte.

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