scholarly journals Evaluation of routine laboratory methods in the diagnosis of tubercular lymphadenitis

1997 ◽  
Vol 12 (S1) ◽  
pp. 66-67
Author(s):  
P. Narang ◽  
Rahul Narang ◽  
D. K. Mendiratta ◽  
S. M. Sharma ◽  
R. Narang ◽  
...  
2014 ◽  
Vol 53 (3) ◽  
pp. 961-963 ◽  
Author(s):  
John Lee ◽  
Aimee Murray ◽  
Richard Bendall ◽  
William Gaze ◽  
Lihong Zhang ◽  
...  

TheStaphylococcus intermediusgroup (SIG) includes zoonotic pathogens traditionally associated with dog bites. We describe a simple scheme for improved detection of SIG using routine laboratory methods, report its effect on isolation rates, and use sequencing to confirm that, apart from one atypical SIG strain, most isolates areStaphylococcus pseudintermedius.


1998 ◽  
Vol 6 (A) ◽  
pp. A279-A284 ◽  
Author(s):  
Gyula Domján ◽  
János Jákó ◽  
István Vályi-Nagy ◽  
Károly Kaffka

The level of cholinesterase in the human blood is a very good indicator of liver function. In this study we desrcibe the relationship between cholinesterase values obtained with routine laboratory methods and near infrared (NIR) spectral data of 72 individuals with a wide range of cholinesterase levels. NIR spectra were measured with a SPECTRALYZER 10–25 (PMC) computerised research analyser. The relationship of laboratory data and values of the second derivative of the log (1/ TF) spectra measured at different wavelengths was determined with multiple linear regression (MLR) analysis using three-term linear summation equations. A correlation coefficient ( r) of 0.89 and a standard error of calibration ( SEC) of 987 units L−1 (U/L) of the enzyme were obtained. Our results indicate that NIR measurements of cholinesterase in the human blood serum can be related to the analytical data obtained with routine laboratory methods. NIR spectroscopy is a rapid, accurate, and inexpensive method for determining various constituents in the human blood.


2016 ◽  
Vol 9 (2) ◽  
pp. 160
Author(s):  
Reyhaneh Alikhani ◽  
Mohsen Shahriari ◽  
Sina Mobasherizadeh

<p>This study aims to evaluate the effect of the protocol for the preparation of oxygen-moisturizing chamber on the count of its bacteria.</p><p>This study is a double- group clinical trial before and after intervention, which was carried out in the neurology ward of an educational hospital in Esfahan from July to December 2015. In this study, 64 oxygen humidifying chambers were studied in terms of number and type of microorganisms in two groups of 32 during three phases, before, immediately after, and 6 hours after its intervention. In order to identify the microorganisms, the samples were cultured on the blood agar and EMB (Eosin Methylene Blue). Then, the routine laboratory methods were used to identify the types of microorganisms. Data were analyzed using SPSS 18, Wilcoxon, Mann-Whitney, chi-square and Friedman statistical tests.</p><p>The results showed that before intervention, 71.9% (46/64) of oxygen-moisturizing chambers were contaminated with microorganisms. The extent of microbial contamination was from 0 to 10<sup>5</sup> CFU. Most of the contamination was with microorganisms such as Lactobacillus spp 23.4% (15/64), Bacillus sp17.2% (11/64), <em>Pseudomonas aeruginosa </em>10.9% (7/64), Coagulase-negative staphylococci 7.8% (5/64), <em>Acinetobacter </em><em>baumannii</em> 3.1% (2/64), Sphingomonas spp 3.1%(2/64), <em>Escherichia coli</em> 1.6% (1/64), Streptococcus spp 3.1% (2/64), Fungus spp 3.1% (2/64). However, immediately after the intervention and 6 hours after connecting oxygen-moisturizing chamber to the patient, the infection rate was substantially reduced and reached zero (p&lt;0/001).</p><p>With regard to the fact that the contamination of oxygen-moisturizing chamber was significantly reduced after the implementation of care protocol, the implementation of this Protocol can be one of the effective measures in reducing the transmission of nosocomial infections.</p>


Author(s):  
J. Taylor ◽  
Joyce L. Bell

An assessment of the performance of the IL 368 Clinicard Analyser is given. The methods tested were urea, sugar, bilirubin, aspartate transaminase and creatine kinase. Overall a good precision and correlation with the routine laboratory methods was found. The machine should prove useful for the analysis of frequently requested emergency determinations.


1928 ◽  
Vol s2-72 (286) ◽  
pp. 277-300
Author(s):  
VISHWA NATH

1. The oogenesis of the spider Crossopriza has been worked out by fresh cover-slip preparations stained with neutral red and Janus green B or kept in 2 per cent, osmic acid from ten minutes to half an hour. Routine laboratory methods have also been used. 2. Treatment with 2 per cent. osmic acid for the period mentioned above does not introduce any artifacts. 3. In the youngest oocyte the Golgi elements are in the form of vacuoles containing a watery and non-fatty fluid, and are embedded in the mitochondrial mass. 4. The solid granular Golgi elements are artifacts produced by the excessive precipitation of metallic silver or osmium inside the vacuoles. 5. The crescent-shaped Golgi elements are also artifacts produced possibly by the incomplete blackening of vacuoles. More probably, however, the crescents are the optical sections of the vacuoles. 6. A process of growth and deposition of fat not miscible with the general cytoplasm inside the Golgi vacuoles gives rise to the fatty yolk-vacuoles. 7. The mitochondria are granular and form a horse-shoe-shaped cap on one side of the nucleus of the youngest oocyte. The cap gradually grows into a complete circum-nuclear ring. The ring breaks up, and ultimately the mitochondria are distributed uniformly throughout the cytoplasm. 8. There are no nucleolar extrusions. The albuminous yolk arises independently in the cytoplasm. 9. Experiments with the centrifuge have been performed. 10. The earlier literature on the origin of fatty yolk has been reviewed. 11. There is no structure in the egg of Crossopriza comparable to the ‘yolk-nucleus’ of the spider Tegenaria described by earlier writers.


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