Gluconic acid production and phosphate solubilization by the plant growth-promoting bacterium Azospirillum spp.

2004 ◽  
Vol 91 (11) ◽  
pp. 552-555 ◽  
Author(s):  
Hilda Rodriguez ◽  
Tania Gonzalez ◽  
Isabel Goire ◽  
Yoav Bashan
Keyword(s):  
Plant Growth ◽  
Gluconic Acid ◽  
Acid Production ◽  
Phosphate Solubilization ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Gluconic Acid Production

scholarly journals Genomic characterization and computational phenotyping of nitrogen-fixing bacteria isolated from Colombian sugarcane fields

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Luz K. Medina-Cordoba ◽  
Aroon T. Chande ◽  
Lavanya Rishishwar ◽  
Leonard W. Mayer ◽  
Lina C. Valderrama-Aguirre ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Nitrogen Fixation ◽  
Plant Growth ◽  
Phosphate Solubilization ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Promising Tool ◽  
A Genome ◽  
Plant Growth Promoting ◽  
Growth Promoting

AbstractPrevious studies have shown the sugarcane microbiome harbors diverse plant growth promoting microorganisms, including nitrogen-fixing bacteria (diazotrophs), which can serve as biofertilizers. The genomes of 22 diazotrophs from Colombian sugarcane fields were sequenced to investigate potential biofertilizers. A genome-enabled computational phenotyping approach was developed to prioritize sugarcane associated diazotrophs according to their potential as biofertilizers. This method selects isolates that have potential for nitrogen fixation and other plant growth promoting (PGP) phenotypes while showing low risk for virulence and antibiotic resistance. Intact nitrogenase (nif) genes and operons were found in 18 of the isolates. Isolates also encode phosphate solubilization and siderophore production operons, and other PGP genes. The majority of sugarcane isolates showed uniformly low predicted virulence and antibiotic resistance compared to clinical isolates. Six strains with the highest overall genotype scores were experimentally evaluated for nitrogen fixation, phosphate solubilization, and the production of siderophores, gibberellic acid, and indole acetic acid. Results from the biochemical assays were consistent and validated computational phenotype predictions. A genotypic and phenotypic threshold was observed that separated strains by their potential for PGP versus predicted pathogenicity. Our results indicate that computational phenotyping is a promising tool for the assessment of bacteria detected in agricultural ecosystems.

scholarly journals Exploring the Multi-trait Plant Growth Promotion Capability of Commercial Liquid Biofertilizers Isolates

2015 ◽  
Vol 9 (3) ◽  
pp. 24-37 ◽  
Author(s):  
Mohammed Faisal Ansari ◽  
Devayani R. Tipre ◽  
Shailesh R. Dave
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Multiple Traits ◽  
The Past ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Field Efficiency

Organic farming is gaining popularity where bio-inoculants could play a key role in promoting the growth of plants. The liquid biofertilizers concept is new to farmers and developed recently. Lots of liquid biofertilizers formulations and field efficiency were shown in the past by various researchers, but the plant growth promoting (PGP) efficiency of the liquid biofertilizers isolates were not reported till date. In the present work 6 different commercially available liquid biofertilizers were used to isolate the organism. These isolated cultures were used to study their PGP efficiency with respect to phosphate solubilization and production of EPS, IAA, siderophore, ammonia, chitinase, ACC-deaminase and HCN. The phosphate solubilization was shown up to 303 g/ml by APS isolate. EPS production was shown by using different C sources and production up to 24 g/l was shown by studied isolated. Most of the organisms studied were able to produce IAA and highest production was shown up to 20 g/ml. More than 65% studied isolates showed siderophore and ACC-deaminase production. The present study shows that the commercial liquid biofertilizer isolates possess multiple traits of plant growth promotion. DOI: http://dx.doi.org/10.3126/ijls.v9i3.12463   International Journal of Life Sciences 9 (3): 2015; 24-37

scholarly journals Mechanism of phosphate solubilization and antifungal activity of Streptomyces spp. isolated from wheat roots and rhizosphere and their application in improving plant growth

Microbiology ◽  
2014 ◽  
Vol 160 (4) ◽  
pp. 778-788 ◽  
Author(s):  
Rahul Jog ◽  
Maharshi Pandya ◽  
G. Nareshkumar ◽  
Shalini Rajkumar
Keyword(s):  
Gene Expression ◽  
Antifungal Activity ◽  
Plant Growth ◽  
Potential Field ◽  
Phosphate Solubilization ◽  
Isocitrate Lyase ◽  
Malate Synthase ◽  
Plant Growth Promoting Activities ◽  
Plant Growth Promoting ◽  
Growth Promoting

The application of plant-growth-promoting rhizobacteria (PGPR) at field scale has been hindered by an inadequate understanding of the mechanisms that enhance plant growth, rhizosphere incompetence and the inability of bacterial strains to thrive in different soil types and environmental conditions. Actinobacteria with their sporulation, nutrient cycling, root colonization, bio-control and other plant-growth-promoting activities could be potential field bio-inoculants. We report the isolation of five rhizospheric and two root endophytic actinobacteria from Triticum aestivum (wheat) plants. The cultures exhibited plant-growth-promoting activities, namely phosphate solubilization (1916 mg l−1), phytase (0.68 U ml−1), chitinase (6.2 U ml−1), indole-3-acetic acid (136.5 mg l−1) and siderophore (47.4 mg l−1) production, as well as utilizing all the rhizospheric sugars under test. Malate (50–55 mmol l−1) was estimated in the culture supernatant of the highest phosphate solublizer, Streptomyces mhcr0816. The mechanism of malate overproduction was studied by gene expression and assays of key glyoxalate cycle enzymes – isocitrate dehydrogenase (IDH), isocitrate lyase (ICL) and malate synthase (MS). The significant increase in gene expression (ICL fourfold, MS sixfold) and enzyme activity (ICL fourfold, MS tenfold) of ICL and MS during stationary phase resulted in malate production as indicated by lowered pH (2.9) and HPLC analysis (retention time 13.1 min). Similarly, the secondary metabolites for chitinase-independent biocontrol activity of Streptomyces mhcr0817, as identified by GC-MS and 1H-NMR spectra, were isoforms of pyrrole derivatives. The inoculation of actinobacterial isolate mhce0811 in T. aestivum (wheat) significantly improved plant growth, biomass (33 %) and mineral (Fe, Mn, P) content in non-axenic conditions. Thus the actinobacterial isolates reported here were efficient PGPR possessing significant antifungal activity and may have potential field applications.

Plant growth promoting fluorescent Pseudomonas enhancing growth of sunflower crop

2015 ◽  
Vol 1 (1) ◽  
Author(s):  
Sakshi Tewari ◽  
Naveen Kumar Arora
Keyword(s):  
Plant Growth ◽  
Seed Treatment ◽  
Phosphate Solubilization ◽  
Dry Weight ◽  
Fluorescent Pseudomonas ◽  
Plant Growth Promoting ◽  
Sunflower Crop ◽  
Growth Promoting ◽  
Semi Arid Region

<p>Ten bacterial isolates were obtained from the rhizosphere of sunflower crop grown in the semi-arid region of west Kanpur.<br />Isolates were further characterized on the basis of morphological, biochemical and physiological characteristics<br />suggesting them to be the member of group fluorescent pseudomonas. Isolates were further monitored for plant growth<br />promoting traits including IAA, phosphate solubilization, siderophore, nitrogen fixation, HCN, chitinase and β-1-3<br />glucanase activity. Amongst all the isolates, PF17 displayed maximum PGP attributes hence it was selected for doing<br />further in vivo pot study taking sunflower as a test crop. Seed treatment with fluorescent pseudomonas PF17 brought<br />enhancement in root length, shoot length, dry weight and seed yield of sunflower crop in comparison to control (untreated<br />seeds). Hence it might be concluded from the study that fluorescent pseudomonas PF17 contains large number of PGP<br />attributes, and its application contributed in enhancement of sunflower growth leading to better yield. In addition, ability<br />to enhance growth of sunflower with the help of biological means appears to be of great ecological and economic<br />importance.</p>

scholarly journals Effect of plant growth promoting rhizobacteria (PGPR) in seed germination and root-shoot development of chickpea (Cicer arietinum L.) under different salinity condition

2016 ◽  
Vol 3 (1) ◽  
pp. 105-113 ◽  
Author(s):  
Mohammad Mosharraf Hossain ◽  
Keshob Chandra Das ◽  
Sabina Yesmin ◽  
Syfullah Shahriar
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Indole Acetic Acid ◽  
Phosphate Solubilization ◽  
Plant Growth Promoting Rhizobacteria ◽  
Matter Production ◽  
Wide Range ◽  
Positive Growth ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth promoting rhizobacteria (PGPR) are beneficial bacteria that colonize plant roots and enhance plant growth by a wide variety of mechanisms. Ten isolates of bacteria designated as SS01, SS02, SS03, SS04, SS05, SS06, SS07, SS08, SS09 and SS10 were successfully isolated and morphologically and biochemically characterized. Subsequently to investigate the effect of PGPR isolates on the growth of chickpea, a pot culture experiment was conducted in 2013 at National Institute Biotechnology, Bangladesh net house. Prior to seeds grown in plastic pots, seeds were treated with PGPR isolates and seedlings were harvested after 21 days of inoculation. All the isolates were gram negative in reaction, catalase positive, produced indole acetic acid (IAA) as well as performed phosphate solubilization, able to degrade cellulose and have the adaptability in wide range of temperature and showed positive growth pattern in medium. Most of isolates resulted in a significant increasing of shoot length, root length and dry matter production of shoot and root of chickpea seedlings. Application of PGPR isolates significantly improves the percentage of seed germination under saline conditions. The present study, therefore suggested that the use of PGPR isolates SS04, SS10 and SS08 as inoculants biofertilizers might be beneficial for chickpea cultivation in saline conditionRes. Agric., Livest. Fish.3(1): 105-113, April 2016

scholarly journals Characterization of Arsenic-Resistant Endophytic Bacteria From Alfalfa and Chickpea Plants

2021 ◽  
Vol 12 ◽  
Author(s):  
Hazhir Tashan ◽  
Behrouz Harighi ◽  
Jalal Rostamzadeh ◽  
Abdolbaset Azizi
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Phosphate Solubilization ◽  
Lipase Production ◽  
Growth Potential ◽  
Sodium Arsenate ◽  
Plant Growth Promoting ◽  
Growth Promoting

The present investigation was carried out to isolate arsenic (As)-resistant endophytic bacteria from the roots of alfalfa and chickpea plants grown in arsenic-contamination soil, characterize their As tolerance ability, plant growth-promoting characteristics, and their role to induce As resistance by the plant. A total of four root endophytic bacteria were isolated from plants grown in As-contaminated soil (160–260-mg As kg−1 of soil). These isolates were studied for plant growth-promoting (PGP) characteristics through siderophore, phosphate solubilization, nitrogen fixation, protease, and lipase production, and the presence of the arsenate reductase (arsC) gene. Based on 16S rDNA sequence analysis, these isolates belong to the genera Acinetobacter, Pseudomonas, and Rahnella. All isolates were found As tolerant, of which one isolate, Pseudomonas sp. QNC1, showed the highest tolerance up to 350-mM concentration in the LB medium. All isolates exhibited phosphate solubilization activity. Siderophore production activity was shown by only Pseudomonas sp. QNC1, while nitrogen fixation activity was shown by only Rahnella sp. QNC2 isolate. Acinetobacter sp. QNA1, QNA2, and Rahnella sp. QNC2 exhibited lipase production, while only Pseudomonas sp. QNC1 was able to produce protease. The presence of the arsC gene was detected in all isolates. The effect of endophytic bacteria on biomass production of alfalfa and chickpea in five levels of arsenic concentrations (0-, 10-, 50-, 75-, and 100-mg kg−1 soil) was evaluated. The fresh and dry weights of roots of alfalfa and chickpea plants were decreased as the arsenic concentration of the soil was increased. Results indicate that the fresh and dry root weights of alfalfa and chickpea plants were significantly higher in endophytic bacteria-treated plants compared with non-treated plants. Inoculation of chickpea plants with Pseudomonas sp. QNC1 and Rahnella sp. QNC2 induced lower NPR3 gene expression in chickpea roots grown in soil with the final concentration of 100-mg kg−1 sodium arsenate compared with the non-endophyte-treated control. The same results were obtained in Acinetobacter sp. QNA2-treated alfalfa plants grown in the soil plus 50-mg kg−1 sodium arsenate. These results demonstrated that arsenic-resistant endophytic bacteria are potential candidates to enhance plant-growth promotion in As contamination soils. Characterization of bacterial endophytes with plant growth potential can help us apply them to improve plant yield under stress conditions.

scholarly journals Isolation and characterization of plant growth-promoting endophytic bacteria of wild legumes growing on sandy soils of Binh Thuan province, Vietnam

2021 ◽  
Vol 10 (3) ◽  
pp. 246-254
Author(s):  
Dang Thi Ngoc Thanh ◽  
Pham Thi Thu Ly ◽  
Pham Thi Nga ◽  
Pham Van Ngot
Keyword(s):  
Nitrogen Fixation ◽  
Plant Growth ◽  
Endophytic Bacteria ◽  
Phosphate Solubilization ◽  
Sandy Soils ◽  
Legume Species ◽  
Bacterial Isolates ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Growth Promoting

The roots of two legume species (Tephrosia purpurea and Tephrosia villosa) that grew wild on dry sandy soils of Binh Thuan province were sources for isolating plant growth-promoting endophytic bacteria. Semi-solid LGI medium was used for the isolation of nitrogen-fixing bacteria from root extracts. All bacterial isolates isolates were evaluated for their ability to solubilize calcium orthophosphate on solid NBRIP medium and their ability to produce IAA in Burk's liquid medium supplemented with 100 mg/L tryptophan. The possibilities of nitrogen fixation, phosphate solubilization and IAA synthesis were all quantitative examined by colorimetric method. Twenty-two bacterial isolates of T. purpurea and 18 isolates of T. villosa were capable of nitrogen fixation in the range of 1.94 to 2.81 mg/L NH4+, whereas only 18 isolates of T. purpurea and 16 isolates of T. villosa showed phosphate solubilization in the range of 12.30 – 48.90 mg/L P2O5, and IAA production in the range of 0.38 – 12.72 mg/L. Sixteen outstanding bacterial isolates of the two legume species were identified by MALDI-TOF technique. The results showed that 13 isolates had high similarity with five bacterial genera including Klebsiella, Cronobacter, Enterobacter, Burkholderia, and Bacillus with score values in the range of 2.070 – 2.411.

scholarly journals A Bacterium Isolated From Soil in a Karst Rocky Desertification Region Has Efficient Phosphate-Solubilizing and Plant Growth-Promoting Ability

2021 ◽  
Vol 11 ◽  
Author(s):  
Jinge Xie ◽  
Zongqiang Yan ◽  
Guifen Wang ◽  
Wenzhi Xue ◽  
Cong Li ◽  
...  
Keyword(s):  
Plant Growth ◽  
Gluconic Acid ◽  
Lateral Roots ◽  
Utilization Efficiency ◽  
Phosphate Solubilizing Bacteria ◽  
Karst Rocky Desertification ◽  
Rocky Desertification ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Phosphate Solubilizing

Phosphorus in the soil accessible to plants can easily be combined with calcium ion, the content of which is high in karst rocky desertification (KRD) regions, thereby resulting in a low utilization efficiency of phosphorus. The application of phosphate-solubilizing bacteria (PSB) from the KRD region would facilitate enhanced phosphate availability in the soil. In the present study, the strains belonging to Acinetobacter, Paraburkholderia, and Pseudomonas with efficient phosphate-solubilizing ability were isolated from fruit tree rhizosphere soils in KRD regions. Particularly, Acinetobacter sp. Ac-14 had a sustained and stable phosphate-solubilizing ability (439–448 mg/L, 48–120 h). Calcium carbonate decreased the phosphate-solubilizing ability in liquid medium; however, it did not affect the solubilization index in agar-solidified medium. When cocultivated with Arabidopsis thaliana seedling, Ac-14 increased the number of lateral roots, fresh weight, and chlorophyll content of the seedlings. Metabolomics analysis revealed that Ac-14 could produce 23 types of organic acids, majorly including gluconic acid and D-(-)-quinic acid. Expression of Ac-14 glucose dehydrogenase gene (gcd) conferred Pseudomonas sp. Ps-12 with a sustained and stable phosphate-solubilizing ability, suggesting that the production of gluconic acid is an important mechanism that confers phosphate solubilization in bacteria. Moreover, Ac-14 could also produce indole acetic acid and ammonia. Collectively, the isolated Ac-14 from KRD regions possess an efficient phosphate-solubilizing ability and plant growth-promoting effect which could be exploited for enhancing phosphorus availability in KRD regions. This study holds significance for the improvement of soil fertility and agricultural sustainable development in phosphorus-deficient KRD regions.

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