Association mapping of grain color, phenolic content, flavonoid content and antioxidant capacity in dehulled rice

2010 ◽  
Vol 122 (5) ◽  
pp. 1005-1016 ◽  
Author(s):  
Yafang Shao ◽  
Liang Jin ◽  
Gan Zhang ◽  
Yan Lu ◽  
Yun Shen ◽  
...  
Keyword(s):  
Association Mapping ◽  
Antioxidant Capacity ◽  
Phenolic Content ◽  
Flavonoid Content ◽  
Grain Color

scholarly journals Genetic Variation And Association Mapping of Phenolic, Flavonoid Content And Antioxidant Capacity In USDA Rice Mini-Core Collection

2021 ◽  
Author(s):  
Qingzhu Li ◽  
Yongyi Ge ◽  
Lily Yan Wang ◽  
Kehu Li
Keyword(s):  
Association Mapping ◽  
Antioxidant Capacity ◽  
Core Collection ◽  
Phenolic Content ◽  
Total Phenolics ◽  
Genotypic Variation ◽  
Genotypic Diversity ◽  
Flavonoid Content ◽  
Mini Core Collection ◽  
Genome Wide

Abstract Genotypic diversity of total phenolics, flavonoid content and antioxidant capacity in the USDA rice mini-core collection was analyzed. Wide genotypic variation was found in total phenolics, flavonoid content and ABTS antioxidant capacity. Genome-wide association mapping between the three antioxidant traits and 155 SSR markers was conducted using Q + K model which takes both population structure (Q) and relative kinship (K) into consideration. A total of 23 marker trait association were identified with markers from Rc gene showed the strongest association with the three antioxidant traits. Rid12, RM484, RM162, RM5371 were commonly detected for phenolic content, flavonoids content and antioxidant capacity.

scholarly journals Proximate and Biological Activity Studies of Syzygium samarangense: Study of different extractives of Syzygium samarangense

2021 ◽  
Vol 3 (1) ◽  
pp. 172-177
Author(s):  
Mohammad Shoeb ◽  
Keyword(s):  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ash Content ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Methanolic Extracts ◽  
Total Antioxidant ◽  
Polycyclic Aromatic ◽  
Red Variety

Fruits of red and green varieties of Syzygium samarangense, commonly known as Jamrul in Bangladesh, are very popular for all ages of people. Air-dried fruits of S. samarangense were successively extracted with n-hexane, dichloromethane (DCM) and methanol. The moisture and ash content of the raw fruits were determined by thermal heating at 105 and 700, respectively. Different biologically important functional groups were identified by FT-IR spectroscopy. The n-hexane, DCM and methanolic extracts were tested for cytotoxicity against HeLa and Vero cell lines. Total phenolic content, total flavonoid content and total antioxidant capacity in the three extracts of both varieties were determined. The moisture content and ash content were 92% and 90%, and 0.12% and 0.16% in red variety and green variety, respectively. UV-Vis spectrum revealed the presence of long chain conjugation or polycyclic aromatic chromophores in n-hexane, DCM and methanolic extracts of S. samarangense. The DCM extract of both varieties had highest total phenolic content, flavonoid content and antioxidant capacity compared to n-hexane and methanol extracts. The study concludes that the fruit of S. samarangense (red and green variety) grown in Bangladesh are rich source of phytochemicals which possess antioxidant activity and safe to health for their non-cytotoxic properties. The green variety contains more phenolic content and antioxidant capacity than red variety.

scholarly journals Study on Total Phenolic, Flavonoid and Antioxidant Capacity of Fish Singgang Extracts

2021 ◽  
pp. 12-21
Author(s):  
Anis Nafisah Jamain ◽  
Norhaslinda Ridzwan ◽  
Mimie Noratiqah Jumli ◽  
Norhayati Abd Hadi ◽  
Mohd Adzim Khalili Rohin ◽  
...  
Keyword(s):  
Moisture Content ◽  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Control Sample ◽  
Ash Content ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content

Aim: To evaluate the ash and moisture contents, total phenolic content, total flavonoid content, and antioxidant potential of Terengganu singgang extracts. Study Design: Experimental study. Place and Duration of Study: Central Laboratory, Tissue Culture Laboratory, Universiti Sultan Zainal Abidin, Terengganu between April 2019 and July 2019. Methodology: Samples comprised three types of singgang dishes, which were prepared, cooked, and then extracted with distilled water and ethanol (EtOH) in different strengths, 50%, 70%, and 100%. These singgang samples were chub mackerel (ST), Indian mackerel (SK), and a control sample with no fish(SC). Extracts were analyzed for their moisture and ash content. Also, the total phenolic content (TPC) was assayed using Folin-Ciocalteu reagent, while total flavonoid content (TFC) using AlCl3 colorimetric assay, and antioxidant activity using 1,1-Diphenyl-2-picrylhydrazyl (DPPH). The total antioxidant capacity (T-AOC) was also evaluated. Results: Experimental assays showed that the SC sample extracted in 100% EtOH produced the highest yield (3.7%). SK samples were lower than SC and ST in moisture content and ash content with 94.21%, 96.37% and 93.03% moisture content and 0.85%, 0.71%, and 0.96% ash content. Meanwhile, the extract of ST in 100% EtOH yielded the highest TPC (315.0 mg GAE/100g) and T-AC (8.8 U/mL) but the lowest in DPPH scavenging activity (12.2%). On the other hand, the extract of SK in 70% EtOH gave the highest TFC with 6485.3 mg QE/100g. The correlation of TFC and TPC with DPPH and T-AOC assays was positively significant. Conclusion: In conclusion, the ST extract yielded the best antioxidant capacity.

scholarly journals Antioxidant, Total Phenolic and Flavonoid Content of Selected Nigerian Medicinal Plants

2015 ◽  
Vol 14 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Matthias Onyebuchi Agbo ◽  
Philip Felix Uzor ◽  
Uchenna Nneamaka Akazie Nneji ◽  
Chidozie Uzoma Eze Odurukwe ◽  
Uchenna Basilia Ogbatue ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Total Antioxidant ◽  
Phenolic And Flavonoid

Plant phenolics and flavonoids play a great role in scavenging free radicals in the body and act as antioxidants. Thus their determination is sometimes nedded. Total antioxidant capacity, total phenolic and flavonoid contents of the extracts of 10 medicinal plants (Lochnera rosea, Allamanda cathartica, Asplenium platyneuron, Euphorbia prostrate, Baphia nitida, Crotolaria retusa, Zapoteca portoricensis, Platycerium bifurcatum, Mussaenda afzelii and Craterosiphon scandens) from 7 botanical families growing in the tropical rainforest of Nigeria were included in this study. The total antioxidant capacity of the extracts was assessed by using the phosphomolybdate method. The phenolic content was determined by using Folin-Ciocalteau assay, while the total flavonoid was determined by the aluminium chloride colorimetric assay. The results obtained showed that the total antioxidant capacity for all the extracts were in the range of 0.888 ± 0.75 to 0.938 ± 0.00 mg EAA/g. The results showed that E. prostrate, P. bifurcatum and A. platyneuron were found to be the richest source of phenolic (97.77 ± 0.77, 87.62 ± 1.22 and 82.33 ± 0.30 mg GAE/g) while B. nitida and M. afzelii had the least total phenolic content (11.67 ± 0.09 and11.18 ± 0.30 mg GAE/g). The highest total flavonoid content was revealed in P. bifurcatum (648.67 ± 12.3 mg QE) while M. afzelii also had the least total flavonoid content (3.67 ± 0.00 mg QE/g). The ratio of flavonoid to the phenolic in each extract was also determined to ascertain extracts that are rich in flavonoids.Dhaka Univ. J. Pharm. Sci. 14(1): 35-41, 2015 (June)

scholarly journals Optimization of Uruset Apple Vinegar Production Using Response Surface Methodology for the Enhanced Extraction of Bioactive Substances

Foods ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 107 ◽  
Author(s):  
Seydi Yıkmış
Keyword(s):  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Process Conditions ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Bioactive Substances ◽  
Total Antioxidant

In this study, the aim is to produce non-thermal vinegar by using red Uruset apples, which have high bioavailability among apple varieties. For this purpose, Uruset apple vinegar was produced and ultrasound at different times (2, 4, 6, 8 and 10 min) and different amplitudes (40%, 50%, 60%, 70%, and 80%); in addition, a 26 kHz frequency was applied to the samples. Total phenolic content (TPC), total flavonoid content (TFC), total antioxidant capacity (1,1-diphenyl- 2-picrylhydrazyl (DPPH) and cupric reducing antioxidant capacity (CUPRAC)), and color values were evaluated for the optimization of process conditions. At the same time, the differences between commercial apple vinegar (CV), pasteurized Uruset apple vinegar (PV), and a control (C) of untreated apple vinegar were investigated. Ultrasound treatment of Uruset apple vinegar was more successful for the enrichment of bioactive substances than the other samples. At the end of the study, the maximal optimization values for Uruset apple vinegar were 7.4 min and 62.2 amplitude. At the end of optimization, CUPRAC (0.69 mg TEAC/mL), DPPH (0.49 mg TEAC/mL), total flavonoid content (46.95 mg CE/L), and total phenolic content (124.25 mg GAE/L) were determined. As a result, ultrasound technology was successfully used for Uruset apple vinegar production.

scholarly journals Correlation of polyphenol content and antioxidant capacity of selected teas and tisanes from Brazilian market

2020 ◽  
Vol 23 ◽  
Author(s):  
Camila Bastos Alves ◽  
Edson Silvio Batista Rodrigues ◽  
Douglas Vieira Thomaz ◽  
Adelmo Menezes de Aguiar Filho ◽  
Eric de Souza Gil ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Phenolic Content ◽  
Oxygen Species ◽  
Redox Behavior ◽  
Flavonoid Content ◽  
Polyphenol Content ◽  
Multivariate Statistical ◽  
Total Polyphenol ◽  
Statistical Approaches ◽  
Thermodynamic Feasibility

Abstract In this work, it was evaluated the phenolic content, redox behavior and antioxidant capacity of several selected teas and tisanes from Brazilian market. The samples were classified as simple (single herb) or composed (blend of two or more herbs). In addition, complementary multivariate statistical approaches were used to identify the correlation and interdependence between the amount of major phytocomponents, such as phenols and flavonoids, as well as the antioxidant activity of the products. Results showed that the total polyphenol and total flavonoid concentrations are correlated to thermodynamic feasibility of reactive oxygen species reduction. The statistical modeling differentiated the results datasets in principal components, whose flavonoid content presented itself as the main parameter to segregate data between simple and composed products. On the other hand, the whole polyphenol content, in both teas and tisanes, was more relevant regarding their antioxidant capacity than the flavonoids content. Considering that the manufactures do not display the real amount of each herb in the labels of the composed products, it was not possible to confirm whether the blends of several species lead to a substantial enhancement on their antioxidant capacity. Furthermore, the redox profile and overall polyphenol content suggest that the consumption of green and black teas as well as traditional yerba mate may be worthwhile in preventing ailments associated with oxidative stress in Brazilian population. Notwithstanding, further clinical studies are required to validate this hypothesis.

scholarly journals Characterization and Uptake of Strawberry-Derived Exosome-Like Nanovesicles by Human Aortic Endothelial Cells

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 310-310
Author(s):  
Jessica Danh ◽  
Brandon Canup ◽  
Rami Najjar ◽  
Maureen Meister ◽  
Hamed Laroui ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Aortic Endothelial Cells ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Human Aortic Endothelial Cells ◽  
Dpph Radical Scavenging

Abstract Objectives To characterize strawberry (SB)-derived exosome-like nanovesicles (ELNs), assess the total phenolic content, total flavonoid content, and total antioxidant capacity as well as its uptake by human aortic endothelial cells (HAECs). Methods SB ELNs were extracted using differential centrifugation. After final ultracentrifugation at 100,000 × g for 1 h, pellets were collected and washed in PBS. Characterization was performed using dynamic light scattering measurements. Total phenolic content (TPC) and flavonoid content (TFC) were determined using Folin-Ciocalteu and aluminum chloride, respectively. Antioxidant capacity was determined using the Trolox equivalent antioxidant capacity (TEAC) and ferric reducing ability of plasma (FRAP) assay while free radical scavenging power was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity assay. Cell viability and uptake were assessed in HAECs. Cell viability was measured after 24-h incubation with SB ELNs using MTT reagent. Cell uptake was measured after 12-h incubation with 100 μg/mL coumarin-6 (C-6) labelled SB ELNs. Fluorescent microscopy and flow cytometry were used to detect cellular uptake of C-6 labelled SB ELNs on a LSR II. Results SB ELNs were sized at 119.4 ± 28.3 nm (PDI = 0.29 ± 0.06). TPC and TFC of SB ELNs were 158.9 ± 22.6 μmol GAE/L and 5.1 ± 0.4 μg QE/mL, respectively. Antioxidant capacity of SB ELNs was 211.38 ± 6.3 μmol TE/L and 118.0 ± 7.6 μmol Fe2+/L by TEAC and FRAP, respectively. DPPH radical scavenging capacity was 181.3 ± 2.5 μmol TE/L in SB ELNs. No cytotoxic effects were observed for SB ELNs in HAECs. Uptake of SB ELNs by HAECs was 15.3% higher compared to baseline levels. Conclusions We report, for the first time, the presence of phenolics and flavonoids in the cargo of SB ELNs, SB ELNs antioxidant capacity, and demonstrate their uptake by HAECs. Taken together, these findings support the need to further characterize and explore the antioxidant potential of SB ELNs in vitro and in vivo. Funding Sources None.

scholarly journals Bioresource Utilization of Djulis (Chenopodium formosanum) Biomass as Natural Antioxidants

2020 ◽  
Vol 12 (15) ◽  
pp. 5926
Author(s):  
Po-Hsien Li ◽  
Yung-Jia Chan ◽  
Wen-Chien Lu ◽  
Da-Wei Huang ◽  
Ting-Chieh Chang ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Total Sugar ◽  
Total Flavonoid ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content

Djulis (Chenopodium formosanum) is a yearly, fast-growing, under-utilized pseudo-cereal with a high proportion of biomass content. We used the hulls, which are usually removed from djulis as crop residue, to evaluate the free-radical scavenging and antioxidant capacity of djulis. We studied the antioxidant capacity of ethanol- and water-extracted hulls and roots by using various in vitro methods. Ascorbic acid was the reference sample. The extract samples were used at 200, 400, 600, 800, and 1000 µg/mL. Total sugar content, total phenolic content, and total flavonoid content were assessed. Antioxidant activity was assessed by using the Trolox equivalent antioxidant capacity, ferric reducing antioxidant power, cupric ion reducing antioxidant capacity, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, and N, N-dimethyl-ρ-phenylenediamine. Ethanol- and water-extracted red djulis hulls showed high amounts of total sugar, total phenolic content, total flavonoid content, and antioxidant capacity. Moreover, ethanol- and water-extracted red djulis roots showed moderate antioxidant capacity. However, ethanol- and water-extracted yellow djulis hulls showed limited antioxidant activities. Utilization of the biomass of djulis hulls and roots as natural antioxidant resources may be environmentally friendly and foreseeable.

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