Gibberellic acids promote growth and exopolysaccharide production in Tetraselmis suecica under reciprocal nitrogen concentration: an assessment on antioxidant properties and nutrient removal efficacy of immobilized iron-magnetic nanoparticles

2021 ◽  
Vol 203 (9) ◽  
pp. 5647-5659
Author(s):  
A. Prathipa ◽  
G. Manigandan ◽  
S. Dinesh Kumar ◽  
P. Santhanam ◽  
P. Perumal ◽  
...  
2021 ◽  
Author(s):  
Prathipa A ◽  
Manigandan G ◽  
Dinesh Kumar S ◽  
Santhanam Perumal ◽  
Perumal P ◽  
...  

Abstract The present study was aimed to assess the effect of gibberellic acids to enhance the growth, biomass, pigment, and exopolysaccharides production in Tetraselmis suecica under reciprocal nitrogen concentrations. For this study, the seven types of experimental media (N-P, NL-P/2GA3, N0-P/2GA3, NL-P/4GA3, N0-P/4GA3, NL-P/6GA3, N0-P/6GA3) were prepared include the addition of gibberellic acids under various nitrogen concentrations. The experiment was lasted for 15 days and the cell density, biomass, chlorophyll ‘a’, and exopolysaccharides (EPS) concentration of T. suecica were estimated for every three days. Then the EPS was subjected to the analyses of chemical (carbohydrate, protein, sulfate, and uronic acid), and antioxidant activity. In addition nutrient removal efficiency was evaluated by using different concentration of EPS. The highest DPPH (86.7±0.95 %) and hydroxyl radical activity (85.7±2.48 %) were observed in 2.5 and 1.2 mg/mL of EPS concentration. The immobilized magnetic Fe3O4-EPS nanoparticles (5.0 and 10.0 g/L) have efficiently removed the excessive phosphate (89.5±1.65 %) and nitrate (73.5±1.72 %) from the Litopenaeus vannamei cultured wastewater. Thus, applying gibberellic acids combined with limited nitrogen concentration could produce higher EPS that could exhibit excellent antioxidant activity, and nutrient removal efficacy in the form of Fe3O4-EPS magnetic nanoparticles.


2020 ◽  
pp. 102-138
Author(s):  
Dafne Crutchik Pedemonte ◽  
Nicola Frison ◽  
Carlota Tayà ◽  
Sergio Ponsa ◽  
Francesco Fatone

This chapter gives an overview on the main technologies for nutrient removal from industrial wastewater by focusing on principles and operational parameters of real applications. A plethora of technologies can achieve the nutrients removal from wastewater depending mainly on their concentration and forms; however, biological nitrification and denitrification and chemical precipitation are the most common processes used today to remove nitrogen and phosphorus, respectively. Stripping, adsorption and membrane based processes for nutrients recovery can be economically viable only when nitrogen concentration is higher than 1.5-2 gN/L. On the other hand, phosphorus recovery should always be pursued and struvite crystalization is the most common option that should be evaluated together with biological phosphorus accumulation in sludge or plants for the following post-processing and valorization.


2017 ◽  
Vol 40 (2) ◽  
pp. 77-81
Author(s):  
Zena M. Hamad

     Acetaminophen also called paracetamol is commonly used as analgesic and antipyretic agent which in high doses causes liver and kidney damage in man and animals. Nigella sativa oil have antioxidant properties. Thirty adult male rats were used and randomly divided into three equal groups. Group (A) untreated and served as control group; Group (B) rats were orally intubated (by gavages needle) acetaminophen suspension (150mg/kg B.W). Group (C) rats were given orally acetaminophen suspension (150mg/kg) plus 1ml/kg B.W of Nigella sativa oil for 42 days in both treated group. Fasting blood samples were collected at 21 and 42 days of experiment to study the following parameters:  Serum creatinine concentration and blood urea nitrogen concentration. The results revealed a significant increase of acetaminophen group in serum creatinine and blood urea nitrogen concentrations as compression with GA. Animals treated with Nigella sativa oil plus acetaminophen (C) showed a significant decline in serum creatinine and blood urea nitrogen concentrations. In conclusion, the acetaminophen was effective in induction of oxidative stress and change in some biological markers related to kidney disease. Also it seems that Nigella sativa oil exerts protective actions against the damaging effect of acetaminophen


1992 ◽  
Vol 26 (9-11) ◽  
pp. 2233-2236 ◽  
Author(s):  
S. J. Kang ◽  
W. F. Bailey ◽  
D. Jenkins

A pilot study was conducted to establish feasibility of biological nutrient removal at one of the most advanced wastewater treatments in the U.S. The results of the study revealed technical feasibility of biological phosphorus removal at extremely high F/M (0.84 1/day) and a short hydraulic detention time (2.2 hours) in the first stage activated sludge system. Due to phosphorus limitation of 0.18 mg/l, it was determined that none of the single sludge BNR processes was applicable at this facility. In the second stage, therefore, biological nitrogen removal was achieved with both methanol and primary effluent as a carbon source. Another significant finding was that after acclimation, the primary effluent exhibited the same rate of denitrification as with methanol (2.7 mg NO3− N/gVSS·hr). Further pilot testing revealed correlations among such process variables as detention time, BOD to nitrogen ratio, and effluent nitrogen concentration.


Author(s):  
Dafne Crutchik Pedemonte ◽  
Nicola Frison ◽  
Carlota Tayà ◽  
Sergio Ponsa ◽  
Francesco Fatone

This chapter gives an overview on the main technologies for nutrient removal from industrial wastewater by focusing on principles and operational parameters of real applications. A plethora of technologies can achieve the nutrients removal from wastewater depending mainly on their concentration and forms; however, biological nitrification and denitrification and chemical precipitation are the most common processes used today to remove nitrogen and phosphorus, respectively. Stripping, adsorption and membrane based processes for nutrients recovery can be economically viable only when nitrogen concentration is higher than 1.5-2 gN/L. On the other hand, phosphorus recovery should always be pursued and struvite crystalization is the most common option that should be evaluated together with biological phosphorus accumulation in sludge or plants for the following post-processing and valorization.


Author(s):  
G. I. Romero-Villegas ◽  
V. A. Burboa-Charis ◽  
E. Navarro-López ◽  
M. C. Cerón-García ◽  
F. G. Acién-Fernandez ◽  
...  

2000 ◽  
Vol 41 (9) ◽  
pp. 131-138 ◽  
Author(s):  
P. Chudoba ◽  
R. Pujol

In order to meet the requirements for total nitrogen removal in sensitive area, as specified by the EC Standards, existing high-rate or medium loaded activated sludge plants treating only carbonaceous pollution have to be upgraded. Two attractive technical solutions are proposed. Depending on both the design and operational conditions of the activated sludge plants, all existing treatment facilities will be reused for nutrient removal with either an immobilized nitrifying system implemented directly in the existing activated sludge tank or with an additional up-flow nitrifying biofilter. Both technical solutions enable the effluent total nitrogen concentration to be as low as 10 mg TN/l. When a high or medium loaded activated sludge plant with primary settling tanks is designed for a HRT of more than five hours, the Pegazur immobilized carrier system can be implemented directly in the biological reactors. The existing aeration tank is divided into anoxic and aerated zones, and the nitrifying Biocubes are introduced in the aerated one. Such an upgraded activated sludge system (which did not nitrify before the upgrading) can reach a maximum eliminated loading rate of 0.5 kg NH4–N per m3 of aerated volume per day at 12°C. This solution was used to upgrade the 120,000 p.e. Bellozanne Sewage Treatment Works, Jersey, Channel Islands, UK. When an existing high rate activated sludge plant is designed for a HRT of less than five hours, the activated sludge tank can be transformed into a high-rate denitrifying anoxic reactor, coupled to a nitrifying Biofor up-flow filter. The high-rate anoxic sludge is continuously controlled by an ORP probe in order to ensure both the denitrifying conditions and recycle ratios are at their optimum level. This controlled operation and high water velocities applied to the nitrifying up-flow Biofor enable the operational mode to switch from dry weather conditions to wet weather conditions where the recycle of nitrified liquor is switched off and the high loaded anoxic sludge is temprarily aerated.


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