QuEChERS sample preparation prior to LC-MS/MS determination of opiates, amphetamines, and cocaine metabolites in whole blood

Abstract The effect of low concentrations of lead on pre- and post-natal growth and development is a current concern. We describe a simple method of sample preparation for direct determination of lead in whole blood by Zeeman graphite-furnace atomic absorption spectrometry. This procedure improves analytical precision and accuracy of lead determinations at low concentrations as compared with published furnace data. At blood lead concentrations of 0.25, 1.98, and 3.76 mumol/L, within-run CVs were 3.2%, 1.8%, and 1.4% respectively; between-run CVs were 7.3%, 2.9%, and 2.2%. Accuracy, as demonstrated by analytical recovery, ranged from 99% to 102%. Our reproducibility/accuracy score in the 1989 Quebec interlaboratory comparison program was 96% compared with the target, second best of 66 participating laboratories.

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Determination of cocaine, metabolites and a crack cocaine biomarker in whole blood by liquid–liquid extraction and UHPLC–MS/MS

Forensic Science International ◽

10.1016/j.forsciint.2018.05.030 ◽

2018 ◽

Vol 289 ◽

pp. 165-174 ◽

Cited By ~ 9

Author(s):

Juliana Takitane ◽

Vilma Leyton ◽

Gabriel Andreuccetti ◽

Hallvard Gjerde ◽

Vigdis Vindenes ◽

...

Keyword(s):

Whole Blood ◽

Crack Cocaine ◽

Liquid Extraction ◽

Liquid Liquid Extraction ◽

Cocaine Metabolites

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Rapid and low cost determination of cadmium in whole blood by hydride generation atomic fluorescence spectrometry after ultrasound-assisted sample preparation

Journal of the Brazilian Chemical Society ◽

10.1590/s0103-50532008000700017 ◽

2008 ◽

Vol 19 (7) ◽

pp. 1347-1354 ◽

Cited By ~ 7

Author(s):

Zhongxi Li ◽

Liping Zhou

Keyword(s):

Sample Preparation ◽

Whole Blood ◽

Low Cost ◽

Hydride Generation ◽

Fluorescence Spectrometry ◽

Atomic Fluorescence Spectrometry ◽

Atomic Fluorescence ◽

Ultrasound Assisted ◽

Cost Determination

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Determination of the International Sensitivity Index of a New Near-Patient Testing Device to Monitor Oral Anticoagulant Therapy

Thrombosis and Haemostasis ◽

10.1055/s-0038-1657641 ◽

1997 ◽

Vol 78 (02) ◽

pp. 855-858 ◽

Cited By ~ 25

Author(s):

Armando Tripodi ◽

Veena Chantarangkul ◽

Marigrazia Clerici ◽

Barbara Negri ◽

Pier Mannuccio Mannucci

Keyword(s):

Linear Relationship ◽

Whole Blood ◽

Oral Anticoagulant ◽

Oral Anticoagulant Therapy ◽

Oral Anticoagulants ◽

Calibration Model ◽

Testing Device ◽

Data Points ◽

Near Patient Testing

SummaryA key issue for the reliable use of new devices for the laboratory control of oral anticoagulant therapy with the INR is their conformity to the calibration model. In the past, their adequacy has mostly been assessed empirically without reference to the calibration model and the use of International Reference Preparations (IRP) for thromboplastin. In this study we reviewed the requirements to be fulfilled and applied them to the calibration of a new near-patient testing device (TAS, Cardiovascular Diagnostics) which uses thromboplastin-containing test cards for determination of the INR. On each of 10 working days citrat- ed whole blood and plasma samples were obtained from 2 healthy subjects and 6 patients on oral anticoagulants. PT testing on whole blood and plasma was done with the TAS and parallel testing for plasma by the manual technique with the IRP CRM 149S. Conformity to the calibration model was judged satisfactory if the following requirements were met: (i) there was a linear relationship between paired log-PTs (TAS vs CRM 149S); (ii) the regression line drawn through patients data points, passed through those of normals; (iii) the precision of the calibration expressed as the CV of the slope was <3%. A good linear relationship was observed for calibration plots for plasma and whole blood (r = 0.98). Regression lines drawn through patients data points, passed through those of normals. The CVs of the slope were in both cases 2.2% and the ISIs were 0.965 and 1.000 for whole blood and plasma. In conclusion, our study shows that near-patient testing devices can be considered reliable tools to measure INR in patients on oral anticoagulants and provides guidelines for their evaluation.

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