Antioxidant activities in vitro of ethanol extract from brown seaweed Sargassum pallidum

Crab apple (Malus baccata (L.) Borkh.) was mainly distributed in Europe as an ornamental plant, but the nutritional properties of its edible fruits were not fully revealed. The aim of the current study was to characterize the phytochemical composition of ripen carb apple fruits and to evaluate their nutritional and antioxidant potentials. The fruits were assayed for moisture and ash content, proteins, lipids, carbohydrates, titratable acidity (TA), pH, total phenolic compounds and natural pigments. Among the analyzed carbohydrates cellulose was found in the highest content (6% dw), followed by sugars (sucrose, glucose and fructose) and 1.8 % dw uronic acids. The total chlorophylls and carotenoids contents in their fruits were 6.51 and 4.80 μg/g fw, respectively. Total monomeric anthocyanins were not detected. The highest content of total phenolic compounds (2.67 mg GAE/g fw) was found in 95 % ethanol extract from fruits, while the total flavonoids were relatively low – 0.1 mg QE/g fw. DPPH assay (17.27 mM TE/g fw) and FRAP assay (14.34 mM TE/g fw) demonstrated in vitro antioxidant activities of crabapple. Malus baccata fruits were evaluated as a rich source of dietary fibers and phenolic compounds with significant antioxidant potential that could be used in human nutrition.

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Evaluation of antioxidant activities of ethanol extract from Ligusticum subjected to in-vitro gastrointestinal digestion

Food and Chemical Toxicology ◽

10.1016/j.fct.2017.12.035 ◽

2018 ◽

Vol 119 ◽

pp. 417-424 ◽

Cited By ~ 5

Author(s):

Huifang Ge ◽

Yazhen Chen ◽

Jicheng Chen ◽

Jingjing Tian ◽

Xiaofeng Liang ◽

...

Keyword(s):

Antioxidant Activities ◽

Ethanol Extract ◽

Gastrointestinal Digestion ◽

In Vitro Gastrointestinal Digestion

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Functional Effects of Pomegranate Peel Extracts on Milk: Antibacterial Measurements, Antioxidant Activities, and Photochemical Characterizations

Journal of Biobased Materials and Bioenergy ◽

10.1166/jbmb.2021.2097 ◽

2021 ◽

Vol 15 (5) ◽

pp. 571-579

Author(s):

Rokayya Sami ◽

Ebtihal Khojah ◽

Mona Alharbi ◽

Amina A. M. Al-Mushhin ◽

Mohammed Saeed Alkaltham ◽

...

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging ◽

Water Extract ◽

Ethanol Extract ◽

Inhibition Zone ◽

Pomegranate Peel ◽

Bacteria Growth ◽

Wonderful Variety ◽

Ic50 Values

Consumers are interested in antimicrobial by-products from natural sources. The present study aims to estimate the antibacterial effects of different concentrations and solvent extracts such as methanol, ethanol, and water extract of pomegranate peels with the milk quality application in-vitro. Listeria monocytogens, E. coli, Salmonella, and Staphylococcus aureus were used as bacteria growth indicators in two pomegranate varieties (i.e., Wonderful and Francis). Besides, evaluating somephysical characterization, antibacterial and antioxidant activities. Results showed that the highest inhibition zone of 18.88 mm was observed with 90% ethanol extract of Wonderful variety against Listeria monocytogens, at 0, 12.5, 25, and 50 mg/mL concentrations, while the lowest inhibition zone of 11.29 mm was observed with 90% methanol extract of Francis variety, at 12.5 mg/mL concentration. The milk treated with WPE of Wonderful variety maintained the bacterial growth from 1.19 ±0.29 x 109 to 1.43 x 1011 CFU/mL at 4 °C. Punicalagin was the most abundant polyphenolic compound (165.05–190.43 µg/mL) followed by gallic acid (153.08–177.65 µg/mL), and p-Hydroxybenzoic acid (91.29–92.5 mg/mL). Acetaldehyde (23.09–27.15 PPM), followed by acetoin (9.65–15.33 PPM) were the most predominant volatile compounds. The WPE treated milk maintained the sensory evaluations such as taste, color, texture, and overall acceptance longer time longer time (i.e., 144 h) than FPE treated milk (i.e., 168 h) at 4°C. DPPH and ABTS radical scavenging of capacity (IC50 values) of FPE treated milk were found to be lower than WPE treated milk (i.e., 159.65 µg/mL and 131.87 µg/mL), respectively. While, FPE treated milk reported a higher viscosity content than WPE treated milk (13.11 cP). In conclusion, pomegranate peel extracts especially Wonderful variety may be used to prolong the shelf-life of milk in dairy products manufacturers.

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WS-5 Extract of Curcuma longa, Chaenomeles sinensis, and Zingiber officinale Contains Anti-AChE Compounds and Improves β-Amyloid-Induced Memory Impairment in Mice

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2019/5160293 ◽

2019 ◽

Vol 2019 ◽

pp. 1-16 ◽

Cited By ~ 2

Author(s):

Ju Eun Kim ◽

Abinash Chandra Shrestha ◽

Hyo Shin Kim ◽

Ha Neul Ham ◽

Jun Hyeong Kim ◽

...

Keyword(s):

Memory Impairment ◽

Antioxidant Activities ◽

Curcuma Longa ◽

Senile Plaques ◽

Total Antioxidant Status ◽

Ethanol Extract ◽

Zingiber Officinale ◽

Chaenomeles Sinensis

Alzheimer's disease (AD) is linked to an extensive neuron loss via accumulation of amyloid-beta (Aβ) as senile plaques associated with reactive astrocytes and microglial activation in the brain. The objective of this study was to assess the therapeutic effect of WS-5 ethanol extract in vitro and in vivo against Aβ-induced AD in mice and to identify the extract’s active constituents. In the present study, WS-5 exerted a significant inhibitory effect on acetylcholinesterase (AChE). Analysis by transmission electron microscopy (TEM) revealed that WS-5 prevented Aβ oligomerization via inhibition of Aβ1-42 aggregation. Evaluation of antioxidant activities using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) demonstrated that WS-5 possessed a high antioxidant activity, which was confirmed by measuring the total antioxidant status (TAS). Furthermore, the anti-inflammatory properties of WS-5 were examined using lipopolysaccharide-stimulated BV-2 microglial cells. WS-5 significantly inhibited the lipopolysaccharide–induced production of nitric oxide and two proinflammatory cytokines, TNF-α and IL-6. The memory impairment in mice with Aβ-induced AD was studied using the Morris water maze and passive avoidance test. Immunohistochemistry was performed to monitor pathological changes in the hippocampus and cortex region of the mouse brain. The animal study showed that WS-5 (250 mg/kg) treatment improved learning and suppressed memory impairment as well as reduced Aβ plaque accumulation in Aβ-induced AD. HPLC analysis identified the extract’s active compounds that exert anti-AChE activity. In summary, our findings suggest that WS-5 could be applied as a natural product therapy with a focus on neuroinflammation-related neurodegenerative disorders.

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SCREENING OF PHYTOCHEMICAL, ANTIOXIDANT ACTIVITY AND ANTI-BACTERIAL ACTIVITY OF MARINE SEAWEEDS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2019v11i1.29119 ◽

2019 ◽

Vol 11 (1) ◽

pp. 61

Author(s):

Rajasekar T. ◽

Mary Shamya A. ◽

Jerrine Joseph

Keyword(s):

Total Protein ◽

Antimicrobial Agents ◽

Antioxidant Activities ◽

Standard Procedure ◽

Brown Seaweed ◽

Ethanol Extract ◽

Total Phenol ◽

Total Carbohydrate ◽

Methanol Extracts ◽

Phytochemical Constituents

Objective: Phytochemical is naturally present in the seaweeds which biologically play a significant role. The intention of this study was designed to screen the phytochemical constituents and antimicrobial potential of selected seaweed collected from Rameshwaram and Tuticorin Southern coast of India.Methods: The present study investigated the presence of phytochemical constituents and also total phenol, total carbohydrate and total protein quantity of the brown seaweed. Dictyopteris delicatula, Padina gymnospora, Acanthophora spicifera, Portieria hornemannii and Ulva faciata were extracted with solvents having different polarities like methanol, ethanol, chloroform and water and screened for the phytochemical constituents, total phenol, total carbohydrate, total protein and DPPH with standard procedure. The antibacterial activities of the seaweeds were examined by agar well diffusion method.Results: Among the five seaweeds, U. faciata showed the maximum number of active constituents in the methanol extract likewise P. gymnospora was found to have a number of diligent compounds in ethanol extract. A. spicifera showed minimum compounds in ethanol as well as chloroform extract. Moreover A. spicifera, P. hornemannii have shown the superior quantity of protein and carbohydrate when compared to other species. The scavenging activity of methanol extracts at 5 mg/ml concentration P. hornemannii shows 18.2% and A. spicifera possess 17.1%. In the antibacterial activity, methanol extracts of all the seaweed showed a potential inhibitory activity against B. cereus and P. aeruginosa compared to other pathogens.Conclusion: The crude extract of seaweed manifest preferable antimicrobial and antioxidant activities, hence in the future, it would be good if it is further taken for treatment of human diseases or as new antimicrobial agents to replace synthetic antimicrobial agents.

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In vitro antiviral, anti-inflammatory, and antioxidant activities of the ethanol extract of Mentha piperita L.

Food Science and Biotechnology ◽

10.1007/s10068-017-0217-9 ◽

2017 ◽

Vol 26 (6) ◽

pp. 1675-1683 ◽

Cited By ~ 9

Author(s):

YuXian Li ◽

YiBo Liu ◽

AiQin Ma ◽

Yong Bao ◽

Man Wang ◽

...

Keyword(s):

Antioxidant Activities ◽

Ethanol Extract ◽

Mentha Piperita ◽

Anti Inflammatory

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Antioxidant Activities In Vitro of Extracts from the Fermented Chinese Jujube

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.554-556.1211 ◽

2012 ◽

Vol 554-556 ◽

pp. 1211-1215

Author(s):

Yana Yuan ◽

Ping Ping Zhang ◽

Jin Fu Liu ◽

Li Jin Zhang ◽

Tong Hai Liu

Keyword(s):

Antioxidant Capacity ◽

Active Substance ◽

Antioxidant Activities ◽

Water Extract ◽

Ethanol Extract ◽

Dose Dependence ◽

Human Consumption ◽

Chinese Jujube ◽

Lower Capacity

Chinese Jujube has long been used for human consumption and medicinal purposes in China. The objective of this study was to extract active substance from the powder of fermented Chinese Jujube and to detect their antioxidant capacities. Macroreticular resin was used to separate FJ-1 from 60% ethanol extract, and silica gel column was used for further separation of FJ-2. Precipitation of CPJ was conducted by addition of 95% ethanol to the water extract. The ability to eliminate DPPH•, O2• and •OH of this component was analyzed. Their activities show dose dependence character. FJ-1, FJ-2 show excellent antioxidant capacity on eliminating DPPH• and •OH, but CPJ shows lower capacity. The capacity on eliminating O2• of CPJ is higher than FJ-1, FJ-2 at the same concentration.

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In vitro antioxidant activities of ethanol extract from Enhalus acoroides (L.F.) Royle

Asian Pacific Journal of Tropical Medicine ◽

10.1016/s1995-7645(10)60216-7 ◽

2010 ◽

Vol 3 (11) ◽

pp. 898-901 ◽

Cited By ~ 39

Author(s):

Rengasamy Ragupathi Raja Kannan ◽

Rajasekaran Arumugam ◽

Perumal Anantharaman

Keyword(s):

Antioxidant Activities ◽

Ethanol Extract ◽

Enhalus Acoroides

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Antiskin Cancer and Antioxidant Activities of Formulated Agar from Brown Seaweed Laminaria digitata (Hudson) in Dimethyl Benzanthracene-Induced Swiss Albino Mice

International Journal of Polymer Science ◽

10.1155/2021/9930777 ◽

2021 ◽

Vol 2021 ◽

pp. 1-12

Author(s):

Jeneesha George ◽

A. Thabitha ◽

N. Vignesh ◽

V. Manigandan ◽

R. Saravanan ◽

...

Keyword(s):

Antioxidant Activities ◽

Histopathological Examination ◽

Brown Seaweed ◽

Cancer Control ◽

In Vitro Cytotoxicity ◽

Control Group ◽

High Dose ◽

Laminaria Digitata ◽

Group 2

This study explores the antiskin cancer effect of formulated agar (FA) from Laminaria digitata on dimethyl benzanthracene- (DMBA-) induced skin cancer mice. The agar was extracted and formulated (emulgel), and FA was biochemically characterized. The in vitro cytotoxicity of FA was tested using NTT 3T3 mice fibroblast cells. The mice were divided into 5 groups: group 1 served as control mice, group 2 mice were considered as DMBA-induced cancer control, group 3 mice were FA pretreated (low dose) + DMBA-induced mice, group 4 mice were FA pretreated (high dose) + DMBA-induced mice, and group 5 were positive control + DMBA-induced mice. The behaviour and biochemical markers of cancer were significantly decreased in group 2 (DMBA-induced) mice, which were brought to near normalcy by FA pretreated mice (groups 3 and 4). The levels of p53 and keratin were significantly elevated in group 2 mice and these levels were decreased in 3 and 4 mice as well. The histopathological examination of DMBA-induced mice was shown degenerated cervical patches in the skin, cirrhosis in liver, oedema in the renal tissue, and swollen and damage in cardiac tissue, which were reduced for the mice applied with FA. This confirms that FA pretreatment offered potential antiskin cancer property.

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