Perception of gait motion during multiple lower-limb vibrations in young healthy individuals: a pilot study

Lymphedema is manifested as a chronic swelling arising due to stasis in the lymphatic flow. No cure is currently available. A non-invasive treatment is a 3 week complete decongestive therapy (CDT), including manual lymphatic drainage and compression bandaging to control swelling. As CDT leads to mobilization of several liters of fluid, effects of CDT on hyaluronan clearance (maker for lymphatic outflow), volume regulating hormones, total plasma protein as well as plasma density, osmolality and selected electrolytes were investigated. In this pilot study, we assessed hyaluronan and volume regulating hormone responses from plasma samples of nine patients (three males, six females, aged 55 ± 13 years) with lower limb lymphedema stage II-III, before - and after - CDT. A paired non-parametric test (Wilcoxon) was used to assess hormonal and plasma volume changes. Correlation was tested using Spearman’s correlation. The main findings of this novel study are that lymphedema patients lost volume and weight after therapy. Hyaluronic acid did not significantly change pre- compared to post-CDT. Aldosterone increased significantly after therapy, while plasma renin activity increased, but not significantly. Plasma total protein, density, osmolality and sodium and chloride did not show differences after CDT. To our knowledge, no study has previously investigated the effects of CDT on volume regulating hormones or electrolytes. To identify the time-course of volume regulating hormones and lymphatic flow changes induced by CDT, future studies should assess these parameters serially over 3 weeks of therapy.

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Facilitating manual wheelchair skills following lower limb amputation using a group process: A nested mixed methods pilot study

Australian Occupational Therapy Journal ◽

10.1111/1440-1630.12759 ◽

2021 ◽

Author(s):

Kimberly Charlton ◽

Carolyn Murray ◽

Rose Boucaut ◽

Angela Berndt

Keyword(s):

Mixed Methods ◽

Pilot Study ◽

Lower Limb ◽

Group Process ◽

Lower Limb Amputation ◽

Limb Amputation ◽

Manual Wheelchair

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Turnout in Classical Dance: Is It Possible to Enhance the External Rotation of the Lower Limb by a Myofascial Manipulation? A Pilot Study

Journal of Dance Medicine & Science ◽

10.12678/1089-313x.21.4.168 ◽

2017 ◽

Vol 21 (4) ◽

pp. 168-178 ◽

Cited By ~ 1

Author(s):

Christine Lohr ◽

Tobias Schmidt

Keyword(s):

Pilot Study ◽

Lower Limb ◽

External Rotation ◽

Classical Dance

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The Origin of Plasma-Derived Bacterial Extracellular Vesicles in Healthy Individuals and Patients with Inflammatory Bowel Disease: A Pilot Study

Genes ◽

10.3390/genes12101636 ◽

2021 ◽

Vol 12 (10) ◽

pp. 1636

Author(s):

Emily Jones ◽

Régis Stentz ◽

Andrea Telatin ◽

George M. Savva ◽

Catherine Booth ◽

...

Keyword(s):

Inflammatory Bowel Disease ◽

Pilot Study ◽

16S Rrna ◽

Gene Sequencing ◽

16S Rrna Gene Sequencing ◽

Rrna Gene ◽

Healthy Individuals ◽

Inflammatory Bowel ◽

Rrna Gene Sequencing ◽

Blood Microbiota

The gastrointestinal tract harbors the gut microbiota, structural alterations of which (dysbiosis) are linked with an increase in gut permeability (“leaky gut”), enabling luminal antigens and bacterial products such as nanosized bacterial extracellular vesicles (BEVs) to access the circulatory system. Blood-derived BEVs contain various cargoes and may be useful biomarkers for diagnosis and monitoring of disease status and relapse in conditions such as inflammatory bowel disease (IBD). To progress this concept, we developed a rapid, cost-effective protocol to isolate BEV-associated DNA and used 16S rRNA gene sequencing to identify bacterial origins of the blood microbiome of healthy individuals and patients with Crohn’s disease and ulcerative colitis. The 16S rRNA gene sequencing successfully identified the origin of plasma-derived BEV DNA. The analysis showed that the blood microbiota richness, diversity, or composition in IBD, healthy control, and protocol control groups were not significantly distinct, highlighting the issue of ‘kit-ome’ contamination in low-biomass studies. Our pilot study provides the basis for undertaking larger studies to determine the potential use of blood microbiota profiling as a diagnostic aid in IBD.

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Effect of corvitin on oxygen homeostasis and blood gas transmitters in ischemia-reperfusion of the lower limbs

Regional blood circulation and microcirculation ◽

10.24884/1682-6655-2021-20-2-37-44 ◽

2021 ◽

Vol 20 (2) ◽

pp. 37-44

Author(s):

V. N. Zasimovich ◽

V. V. Zinchuk ◽

N. N. Ioskevich

Keyword(s):

Lower Limb ◽

Ischemia Reperfusion ◽

Venous Blood ◽

Nitrogen Monoxide ◽

Early Postoperative Period ◽

Lower Limbs ◽

Healthy Individuals ◽

Oxygen Homeostasis ◽

Group I ◽

Male Patients

Introduction. Reperfusion-reoxygenation syndrome (RRS) after revascularization of the lower limbs in obliterating atherosclerosis of the arteries is accompanied by a violation of the oxygen transport function of the blood (OTFB) and the content of gas transmitters (GTs). Reperfusion injury affects not only the tissues of the lower limbs, but also of anatomically distant organs, which supposes that effective RRS correction is required. Aim. To study the effect of Corvitin on the OTFB parameters and the content of GT of nitrogen monoxide (NO) and hydrogen sulfide (H2S) in the venous blood of the forearm after revascularization of the lower limb in chronic atherosclerotic occlusion of the superficial femoral artery (SFA). Materials and methods. The study included 118 male patients. Revascularization of the lower limb was carried out by the method of loop endaterectomy from the SFA. Patients of group I (n=52) received traditional medication, 51 patients of group II additionally received Corvitin. In the blood from the vein of the elbow bend before the operation, on the 3rd and 8th days after it, the indices of OTFB and GTs were determined. Results. In group I, on the 3rd day after surgery, pO2 increased in relation to healthy individuals by 5.2–18.5%, while pCO2 decreased by 4.8–6.7%, depending on the stage of initial ischemia. The concentrations of NO and H2S increased by 9.2–50.1% and 9.2–21.1%, respectively. The increase in the parameters of hyperoxemia, hypocapnemia and GT after the return of blood circulation decreases with the use of Corvitin (p˂0.05). By the end of the early postoperative period, the indicators of OTFB and GT not only return to their initial values, but also do not significantly differ from the group of healthy individuals (p˃0.05). Conclusion. The use of Corvitin effectively corrects violations of OTFB and GT during ischemia-reperfusion of the lower limbs, which prevents tissue reperfusion damage.

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