Surface-modified sulfur nanoparticles: an effective antifungal agent against Aspergillus niger and Fusarium oxysporum

2011 ◽  
Vol 90 (2) ◽  
pp. 733-743 ◽  
Author(s):  
Samrat Roy Choudhury ◽  
Mahua Ghosh ◽  
Amrita Mandal ◽  
Dipankar Chakravorty ◽  
Moumita Pal ◽  
...  
Author(s):  
B. Mohana ◽  
Shiva Kameshwari ◽  
M. K. Prasana Kumar

Aqueous extract of Urginea indica kunth. (Udupi acccession) was screened for antifungal activity against Fusarium oxysporum, Sclerotium rolfsii, Magnaporthe orzae, Aspergillus flavus, Alternaria alternata, Aspergillus niger and Fusarium moniliformae by poisoned food technique. The results confirmed Urginea indica extracts showed very significant antifungal activity against Fusarium oxysporum and showed significant inhibition for Sclerotium rolfsii and Magnapothea orzaea it showed no activity against Aspergillus niger and Aspergillus flavus. All the activity was evaluated to determine the lowest concentration required to inhibit visible mycelial growth of the pathogen at minmum concentration. Fusarium oxysporum showed very significant inhibition in 10% concentration (Reconfirmed) while Sclerotium showed significant inhibition in 25% concentration followed by Magnoporthe oryzae. The number of sclerotia spores formed was also reduced drastically. These results show that a potential and safe antifungal agent can be obtained from Urginea.


2006 ◽  
Vol 31 (2) ◽  
pp. 158-163 ◽  
Author(s):  
Derblai Casaroli ◽  
Danton C. Garcia ◽  
Marlove F. B. Muniz ◽  
Nilson L. de Menezes

O trabalho teve como objetivos avaliar e correlacionar a qualidade sanitária e fisiológica de sementes de abóbora, variedade Menina Brasileira (Cucurbita moschata.). Foram avaliados dois lotes de sementes de abóbora produzidas no sistema agroecológico e quatro no sistema convencional, com e sem tratamento químico. Os lotes foram submetidos aos testes de sanidade, seguindo a metodologia do "Blotter test", com congelamento, germinação e vigor (primeira contagem, índice de velocidade de germinação, envelhecimento acelerado e emergência de plântulas). Os resultados indicaram a separação dos lotes de diferentes origens a partir da qualidade sanitária e fisiológica, onde as maiores incidências de fungos foram observadas nos lotes agroecológicos e o maior potencial fisiológico foi observado nos lotes de origem convencional não tratados. Foram encontrados os fungos Fusarium oxysporum, Alternaria alternata, Cladosporium cucumerinum, Aspergillus niger, Penicillium digitatum, Rhizopus stolonifer e Phoma terrestris. A qualidade sanitária não interferiu na qualidade fisiológica das sementes de abóbora, variedade Menina Brasileira.


2017 ◽  
Vol 4 (11) ◽  
pp. 66-71
Author(s):  
Valmik M. Patil ◽  
◽  
Kishor R. Patole ◽  
Mohan S. Paprikar ◽  
Jaysingh C. Rajput ◽  
...  

2012 ◽  
Vol 10 (1) ◽  
pp. 46-50 ◽  
Author(s):  
F. Yeasmin ◽  
M. Ashrafuzzaman ◽  
I. Hossain

Seeds of rice were treated with Garlic (Allium sativum) clove extract @ 1:0, 1:1, 1:2 dilutions in water, allamanda (Allamanda cathartica) leaf extract @ 1:1, 1:2, 1:3 dilutions in water and Provax-200 @ 0.3% for controlling seed borne fungi, where the seed samples of three rice varieties viz. Katharee, Gutee Aus and Kalijira were collected from farmer’s storages of Bangladesh. The seed germination under control ranged from 64 to 77%, where treatments resulted up to 100% germination. The identified seed borne fungi of rice were Bipolaris oryzae, Curvularia oryzae, Fusarium oxysporum, F. moniliforme, Nigrospora oryzae, Aspergillus flavus, Aspergillus niger and Penicillium sp., where prevalence of Bipolaris oryzae (7.5%) and Fusarium moniliforme (8.3%) were the maximum. All the treatments significantly reduced the seed borne fungi up to 100% over the control, where Provax was found best and was statically similar to garlic (1:1) extract against seed borne pathogen of rice.DOI: http://dx.doi.org/10.3329/agric.v10i1.11064The Agriculturists 2012; 10(1): 46-50


2011 ◽  
Vol 4 (1) ◽  
pp. 203 ◽  
Author(s):  
P. Sunita ◽  
S. Jha ◽  
S. P. Pattanayak ◽  
S. K. Mishra

In the present investigation different fractions (hexane, ethylacetate and methanol) of the whole methanolic extract of Cressa cretica L.(Convolulaceae), a widely grown halophytic plant were studied against wide ranges of bacteria (both positive and negative strain) and five fungi  Candida albicans, Candida tropicalis – dimorphic fungi, Aspergillus fumigatus, Aspergillus niger – systemic fungi and fusarium oxysporum by agar disc diffusion method. Among the three fractions the ethylacetate fraction of C.cretica showed the highest activity, but among the pathogens highest activity was revealed against Escherichia coli, Klebsiella pneumoniae  (zone of inhibition diameter in mm was found to be 26 and 31, respectively). The ethylacetate fraction was sensitive to both gram +ve and gram –ve bacterias. C.cretica showed higher inhibitory activity against the Aspergillus fumigates, Aspergillus niger (zone of inhibition diameter in mm was found to be 26 and 22, respectively) than the Candida albicans and Candida tropicalis and least was found for Fusarium oxysporum. As ethylacetate fraction revealed better activity than other fractions, further study of morphological and biochemical alterations were carried out only with ethylacetate fraction and the results revealed some morphological and biochemical alterations, which indicates that ethylacetate fraction of C. cretica possess potential broad spectrum antimicrobial activity.Keywords: Cressa cretica; Antifungal; Gram positive bacteria; Gram negative bacteria; Biochemical alterations.© 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v4i1.7719J. Sci. Res. 4 (1), 203-212 (2012)


2018 ◽  
Vol 9 (2) ◽  
pp. 329-341
Author(s):  
Edgar López López ◽  
Margarita Gisela Peña Ortega ◽  
María Teresa Beryl Colinas León ◽  
Francisco Díaz Cedillo ◽  
Miguel Ángel Serrato Cruz

La especie T. lucida conocida como ‘pericón’, es un recurso natural de México cuyo aceite esencial tiene propiedades antifúngicas, aunque no en todos los hongos fitopatógenos se ha explorado el efecto que puede ocasionar esta sustancia vegetal. De una población recolectada en Atotonilco el Grande, Hidalgo, México se obtuvo aceite esencial por hidrodestilación con rendimiento de 0.2%; mediante la técnica CG-EM se identificaron ocho componentes diferentes en el aceite, pero los abundantes fueron: estragol (48%) y anetol (35%). También se evaluó la actividad antimicótica in vitro del aceite esencial contra Aspergillus niger, Fusarium oxysporum, Penicillium janthinellum y Rhizoctonia solani mediante la técnica de difusión en agar, realizando dos experimentos: el primero ensayando concentraciones de 0, 0.1 y 1% y en el segundo 0, 2 y 3%. La concentración de 1% redujo el crecimiento micelial 46% para F. oxysporum, 39% en R. solani, 21% en A. niger y 16% en P. janthinellum; sin embargo, en concentraciones altas de aceite, como 3%, solo en R. solani se observó la mayor la reducción del crecimiento micelial (72%).


2013 ◽  
Vol 5 (1) ◽  
pp. 50
Author(s):  
Kellem Ângela Oliveira de Sousa ◽  
José Fábio França de Orlanda ◽  
Gustavo De Andrade Bezerra ◽  
Thatyane Pereira De Sousa

RESUMO: O presente trabalho objetivou avaliar a capacidade antimicrobiana in vitro, de diferentes extratos de fungos endofíticos, como alternativa no biocontrole de Fusarium oxysporum f.sp. lycopersici. Foram obtidos oito isolados endofíticos de plantas do Cerrado Maranhense e de interesse econômico, a saber: aroeira (Myracrodruon urundeuva Allemão), jenipapo (Genipa americana L.), milho (Zea Mays L.), mandioca (Manihot esculenta Crantz), vinagreira (Hibiscus sabidariffa L.) e graviola (Annona muricata L.). Os isolados endofiticos das espécies foram identificados em Aspergillus niger e Penicillium italicum, com características morfológicas distintas entre as colônias e todos os isolados endofíticos verificou-se crescimento mediano. Para a atividade antimicrobiana, foi realizada a extração dos metabólitos dos isolados endofiticos com os solventes: Acetato de Etila, Clorofórmio e Diclorometano, posteriormente realizado o bioensaio através do confronto direto do antagonista (endófito) sobre o Fusarium Oxysporum f.sp. lycopersici, raça 1, pelo método de difusão em ágar. Os extratos dos isolados endofíticos obtidos do solvente Clorofórmio apresentaram capacidade inibitória, assim interferindo no crescimento do Fusarium oxysporum f. sp. lycopersici raça. Que foram provenientes do caule milho (II) e de jenipapo, com diâmetros de 13mm e 11mm, respectivamente, os outros extratos dos isolados apresentaram halos de inibição inferior. PALAVRAS-CHAVE: antibiose, controle biológico, fitopatógeno.


2016 ◽  
Vol 23 ◽  
pp. 19-27 ◽  
Author(s):  
Roshan Chhetri ◽  
Sanjay Kumar Jha

Nine hundred sixty seeds of lentils (Lens culinaris) from three different storage sources were tested for seed fungi using Potato Dextrose Agar (PDA) and Blotter technique. Thirty five different fungi were isolated, PDA method being better than Blotter technique. Seeds from traditional village storage were found to be more infested with fungi than source local market and source Nepal Agricultural Research Centre (NARC). The fungal infestation on seeds from village storage was 49.37%, source NARC was 44.37% and source local market was 30.31%. Aspergillus flavus, Aspergillus niger, Alternaria alternata, Penicillium chrysogenum and Fusarium oxysporum were the fungi having higher incidence and frequency. Surface disinfection by 2% NaOCl reduced the fungal incidence and frequency. The aqueous extract of three plants viz, Ageratum houstonianum, Eclipta prostrata and Vitex negundo at 25%, 50% and 100% concentration were assessed in-vitro for antifungal activity against Alternaria alternata, Aspergillus niger, Chaetomium funicola and Fusarium oxysporum. Among these fungi, Alternaria alternata was best controlled by Ageratum houstonianum; Aspergillus niger by Eclipta prostrata; Fusarium oxysporum by Vitex negundo and Ageratum houstonianum. The inhibition increased from lower concentration (25%) to higher (100%).ECOPRINT 23: 19-27, 2016


2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Laila Muñoz Castellanos ◽  
Nubia Amaya Olivas ◽  
Juan Ayala-Soto ◽  
Carmen Miriam De La O Contreras ◽  
Miriam Zermeño Ortega ◽  
...  

In this study, hydrodistillation was used to obtain essential oils (EOs) from pepper (Piper nigrum L.) and clove (Eugenia caryophyllata) and co-hydrodistillation (addition of fatty acid ethyl esters as extraction co-solvents) was used to obtain functional extracts (FEs). Antifungal activity of EOs and FEs was evaluated by determination of minimum inhibitory concentration (MIC) against Fusarium oxysporum and Aspergillus niger. The results showed that pepper (Piper nigrum) and clove (Eugenia caryophyllata) essential oils and their functional extracts are effective in vitro at concentrations from 400 to 500 ppm after 10 days of culturing. The essential oils and functional extracts were used on tomato fruit samples at three different concentrations: 350, 400, and 450 ppm5. Clove essential oil reduced the growth of Aspergillus niger from 50% to 70% and Fusarium oxysporum to 40%. The functional extracts (FEs) of clove and pepper, mixed with ethyl decanoate (FEs-C10), were the best combination for protecting the tomato fruit in vivo against both phytopathogenic fungi. Gas chromatography-mass spectrometry (GC-MS) was used to identify eugenol as the principal compound in clove oil and limonene, sabinene, and β-caryophyllene in pepper oil.


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