scholarly journals Antifungal Activity in Urginea indica Kunth. (Asparagaceae)

2020 ◽  
pp. 18-23
Author(s):  
B. Mohana ◽  
Shiva Kameshwari ◽  
M. K. Prasana Kumar
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Aspergillus Flavus ◽  
Antifungal Agent ◽  
Mycelial Growth ◽  
Sclerotium Rolfsii ◽  
Significant Inhibition ◽  
Poisoned Food Technique ◽  
Urginea Indica

Aqueous extract of Urginea indica kunth. (Udupi acccession) was screened for antifungal activity against Fusarium oxysporum, Sclerotium rolfsii, Magnaporthe orzae, Aspergillus flavus, Alternaria alternata, Aspergillus niger and Fusarium moniliformae by poisoned food technique. The results confirmed Urginea indica extracts showed very significant antifungal activity against Fusarium oxysporum and showed significant inhibition for Sclerotium rolfsii and Magnapothea orzaea it showed no activity against Aspergillus niger and Aspergillus flavus. All the activity was evaluated to determine the lowest concentration required to inhibit visible mycelial growth of the pathogen at minmum concentration. Fusarium oxysporum showed very significant inhibition in 10% concentration (Reconfirmed) while Sclerotium showed significant inhibition in 25% concentration followed by Magnoporthe oryzae. The number of sclerotia spores formed was also reduced drastically. These results show that a potential and safe antifungal agent can be obtained from Urginea.

scholarly journals In Vitro Studies of Antifungal Activity of Colloidal Silver against Important Plants Pathogens

2018 ◽  
Vol 46 (2) ◽  
pp. 533-537 ◽  
Author(s):  
Oana VENAT ◽  
Beatrice IACOMI ◽  
Adrian G. PETICILĂ
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Botrytis Cinerea ◽  
Aspergillus Flavus ◽  
Mycelial Growth ◽  
Fusarium Culmorum ◽  
Penicillium Digitatum ◽  
Alternaria Brassicicola ◽  
Colloidal Silver

Colloids and especially silver are increasingly used in a variety of worldwide applications because of their potential antimicrobial activity and their plasmotic and conductivity properties. This research reports the fungitoxic properties of colloidal silver on mycelial growth of important plant pathogens: Alternaria brassicicola, Botrytis cinerea, Aspergillus flavus, Aspergillus niger, Fusarium culmorum, Fusarium oxysporum, Penicillium digitatum and Sclerotinia sclerotiorum. Although variable responses towards each compound were observed within the species the results revealed a clear reaction to limiting mycelium growth relative to various concentration of Colloidal silver (CS). Results were expressed as effective concentrations which inhibit mycelial growth by 50% and 90% respectively (EC50 and EC90). Efficiency of colloidal silver on mycelial growth inhibition of different isolates based on EC50 have the following values: 3.69 ppm for Alternaria brassicicola, 7.32 ppm for Botrytis cinerea, 18.21 ppm for Aspergillus flavus, 10.43 ppm for Aspergillus niger, 11.99 ppm for Fusarium culmorum, 12.27 ppm for Fusarium oxysporum, 10.82 ppm for Penicillium digitatum and 6.34 ppm for Sclerotinia. According to the obtained results the antifungal activity of colloidal silver particles as biocide has potential for using it as a non-aggressive treatment in horticulture and sustainable horticulture.

scholarly journals Evaluation of antifungal activity of seaweed extract (Turbinaria conoides) against Fusarium oxysporum

2016 ◽  
Vol 8 (1) ◽  
pp. 60-62 ◽  
Author(s):  
Ameer Junaithal Begum M. ◽  
P. Selvaraju ◽  
A. Vijayakumar
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Root Rot ◽  
Mycelial Growth ◽  
Tamil Nadu ◽  
Seaweed Extract ◽  
Antimicrobial Compounds ◽  
Turbinaria Conoides ◽  
Poisoned Food Technique ◽  
Anti Fungal

The purpose of the study was to determine the anti fungal activity of seaweed (Turbinaria conoides) extract against root rot pathogen Fusarium oxysporum. Seaweed extract was prepared from the species T. conoides collected from Rameswaram coastal area of Tamil Nadu during December was used for this study. Different concentrations of the extract viz., 5 %, 10 %, 15 % and 20 % was evaluated for their antifungal activity against F. oxysporum using poisoned food technique along with control and carbendazim (0.2 %) as check. No mycelial growth (0 cm) was observed in 15 % and 20 % sea weed extract weed extract treated plates even after 6 days ofincubation. Though the visible inhibition of mycelial growth was noticed in all the concentrations, the increased concentration of 15 and 20 % had shown 100 % inhibition. So, the lower concentration of 15 % can be best in controlling the F. oxysporum fungi. GC-MS analysis of seaweed extract showing the presence of several antimicrobial compounds in seaweeds may be the reason for such inhibition.

scholarly journals Chemical composition and antifungal activity of Mentha spicata L. plant from Sulaimaniyah in Iraq

2017 ◽  
Vol 2 (1) ◽  
pp. 52-56
Author(s):  
Lana MA. Mohammed ◽  
Tara Faeq M. Salah ◽  
Karzan O. Qader
Keyword(s):  
Chemical Composition ◽  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Mycelial Growth ◽  
Significant Inhibition ◽  
Plant Sample ◽  
Mentha Spicata ◽  
Aerial Parts ◽  
Total Plant ◽  
Plants Extract

Aerial parts of Mentha plants were collected from Sulaimaniyah city, during 15th Mar to 1st July2016. Clevenger apparatus was used for plant sample distillation and then obtained essential oils were analyzed by GC and GC/MS. The GC–MS analysis resulted in the detection of thirty-eight compounds which made up 100% of the total plant essential oil. The major components of the oil were (54.44%) of 2-Cyclohexen-1-one, 2-methyl-5-(1- methylethenyl)-, (R)-, (27.72%) of D-Limonene, (3.29%) of 2-Cyclohexen-1-ol, 2-methyl-5-(1- methylethenyl)-, trans-, (2.16%) of Cyclohexanol, 2- methyl-5-(1-methylethenyl)-, (1.83%) of Bicyclo[3.1.0] hexan-2-ol,2-methyl-5-(1-methylethyl)- (1.alpha.,2.beta.,5.alpha.) and (1.03%) of 3- Cyclohexen-1-ol, 4-methyl-1-(1-methylethyl)-, (R)-. The significant inhibition percentages of Mentha spicata plants extract on the Aspergillus niger mycelial growth were 16.78%, 18.92%, 23.21%, 28.57% and36.78%, in 32%, 34%, 36%, 38% and 40% dilution respectively, while other concentrations showed no significant effect.

scholarly journals Effects of Garlic Extract, Allamanda Leaf Extract and Provax -200 on Seed Borne Fungi of Rice

2012 ◽  
Vol 10 (1) ◽  
pp. 46-50 ◽  
Author(s):  
F. Yeasmin ◽  
M. Ashrafuzzaman ◽  
I. Hossain
Keyword(s):  
Aspergillus Niger ◽  
Seed Germination ◽  
Fusarium Oxysporum ◽  
Aspergillus Flavus ◽  
Fusarium Moniliforme ◽  
Leaf Extract ◽  
Allium Sativum ◽  
Bipolaris Oryzae ◽  
Rice Varieties ◽  
Nigrospora Oryzae

Seeds of rice were treated with Garlic (Allium sativum) clove extract @ 1:0, 1:1, 1:2 dilutions in water, allamanda (Allamanda cathartica) leaf extract @ 1:1, 1:2, 1:3 dilutions in water and Provax-200 @ 0.3% for controlling seed borne fungi, where the seed samples of three rice varieties viz. Katharee, Gutee Aus and Kalijira were collected from farmer’s storages of Bangladesh. The seed germination under control ranged from 64 to 77%, where treatments resulted up to 100% germination. The identified seed borne fungi of rice were Bipolaris oryzae, Curvularia oryzae, Fusarium oxysporum, F. moniliforme, Nigrospora oryzae, Aspergillus flavus, Aspergillus niger and Penicillium sp., where prevalence of Bipolaris oryzae (7.5%) and Fusarium moniliforme (8.3%) were the maximum. All the treatments significantly reduced the seed borne fungi up to 100% over the control, where Provax was found best and was statically similar to garlic (1:1) extract against seed borne pathogen of rice.DOI: http://dx.doi.org/10.3329/agric.v10i1.11064The Agriculturists 2012; 10(1): 46-50

scholarly journals Synthesis & Antifungal Screening of Novel Azetidin-2-ones

2015 ◽  
Vol 2 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Apoorva Gupta ◽  
Anand K. Halve
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Aspergillus Flavus ◽  
Elemental Analysis ◽  
Spectroscopic Data ◽  
Activity Relationship ◽  
Synthetic Route ◽  
Chemical Structures ◽  
Structure Activity ◽  
Significant Structure

A new series of 4-[3-chloro-2-(4-hydroxy-3-methoxybenzyllidene)-4-oxoazetidin-1-yl]amino-N-(substituted)benzenesulfonamide, 4-{3-chloro-2-[5-methoxy-2-nitro-4-(prop-2-en-1-yloxy)benz yllidene]-4-oxoazetidin-1-yl}]amino}-N-(substituted)benzenesulfonamide and 4-{3-chloro-2-[4-hydroxy-3-methoxy-5-(prop-2-en-1-yl)benzylidene]-4-oxoazetidin-1-yl}amino}-N-(substituted) benzenesulfonamide were synthesized using appropriate synthetic route. The chemical structures of all the synthesized compounds were deduced on the basis of elemental analysis and spectroscopic data. The antifungal activity of the synthesized compounds was screened against several fungus. The synthesized compounds show potent antifungal activity against Aspergillus niger & Aspergillus flavus and significant structure-activity relationship (SAR) trends.

Anti-Fungal Activity of Cressa cretica, Leptadenia pyrotechnica and Pulicaria crispa, indigenous plants of Cholistan desert, Pakistan

2020 ◽  
Vol 18 ◽  
Author(s):  
Faheem Hadi ◽  
Tahir Maqbool ◽  
Sameera Khurshid ◽  
Aisha Nawaz ◽  
Saira Aftab ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Aspergillus Flavus ◽  
Fungal Infections ◽  
Fungal Species ◽  
Fungal Activity ◽  
Aspergillus Ustus ◽  
Mother Tincture ◽  
Anti Fungal

Background: Fungal infections have always remain a problem and they are getting worse with passage of each year due to their resistance against available antibiotics. Natural ways of treatment with homoeopathic medicines made of mother tinctures of plants have no reported side effects and have been proved effective against many bacterial and fungal infections. Materials & Methods: Preparation of mother tinctures of plants Cressa cretica, Leptadenia pyrotechnica and Pulicaria crispa was done and used to evaluate the anti-fungal potential of these plants against potentially pathogenic fungal species like Aspergillus niger, Aspergillus flavus, Aspergillus ustus and Candida albicans by agar disc diffusion method. Each tincture was evaluated at 0.25 ml, 0.5 ml and 1 ml volume per disc and zone of inhibition was measured in millimetres and compared with commercial drug Fluconazole (2 mg/ml) which was used as standard. Results: The results showed that satisfactory anti-fungal activity of these plants in comparison to standard drug Fluconazole. The ratio of antifungal activity of Cressa cretica measured as the zone of growth inhibition of these cultures against Aspergillus niger, Aspergillus flavus, Aspergillus ustus and Candida albicans was 24 mm / 27 mm, 30mm / 28 mm, 23 mm/ 30 mm and 32 mm/ 30 mm respectively. In the case of Leptadenia pyrotechnica, the mother tincture’s antifungal activity was 32 mm / 27 mm, 30 mm / 28 mm, 17 mm/ 30 mm and 24 mm/ 30 mm. In case of our third plant Pulicaria crispa, antifungal activity came out to be 23 mm/ 27 mm, 26 mm/ 28 mm, 26 mm / 30 mm and 24 mm/ 30 mm. Conclusion: Our study proved that mother tincture of these plants can be a potential new therapy to treat fungal infections and has the potential to rule out fungal problems. Further research using mother tincture of these plants against other fungal species has the potential to prove them a safer and widespread anti-fungal homoeopathic medicine.

scholarly journals In Vitro and In Vivo Antifungal Activity of Clove (Eugenia caryophyllata) and Pepper (Piper nigrum L.) Essential Oils and Functional Extracts Against Fusarium oxysporum and Aspergillus niger in Tomato (Solanum lycopersicum L.)

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Laila Muñoz Castellanos ◽  
Nubia Amaya Olivas ◽  
Juan Ayala-Soto ◽  
Carmen Miriam De La O Contreras ◽  
Miriam Zermeño Ortega ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Essential Oils ◽  
Fusarium Oxysporum ◽  
Tomato Fruit ◽  
Ethyl Esters ◽  
Piper Nigrum ◽  
Fruit Samples

In this study, hydrodistillation was used to obtain essential oils (EOs) from pepper (Piper nigrum L.) and clove (Eugenia caryophyllata) and co-hydrodistillation (addition of fatty acid ethyl esters as extraction co-solvents) was used to obtain functional extracts (FEs). Antifungal activity of EOs and FEs was evaluated by determination of minimum inhibitory concentration (MIC) against Fusarium oxysporum and Aspergillus niger. The results showed that pepper (Piper nigrum) and clove (Eugenia caryophyllata) essential oils and their functional extracts are effective in vitro at concentrations from 400 to 500 ppm after 10 days of culturing. The essential oils and functional extracts were used on tomato fruit samples at three different concentrations: 350, 400, and 450 ppm5. Clove essential oil reduced the growth of Aspergillus niger from 50% to 70% and Fusarium oxysporum to 40%. The functional extracts (FEs) of clove and pepper, mixed with ethyl decanoate (FEs-C10), were the best combination for protecting the tomato fruit in vivo against both phytopathogenic fungi. Gas chromatography-mass spectrometry (GC-MS) was used to identify eugenol as the principal compound in clove oil and limonene, sabinene, and β-caryophyllene in pepper oil.

scholarly journals Potential of Octanol and Octanal from Heracleum sosnowskyi Fruits for the Control of Fusarium oxysporum f. sp. lycopersici

2020 ◽  
Vol 12 (22) ◽  
pp. 9334
Author(s):  
May Khaing Hpoo ◽  
Maryia Mishyna ◽  
Valery Prokhorov ◽  
Tsutomu Arie ◽  
Akihito Takano ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Fusarium Oxysporum ◽  
Volatile Compounds ◽  
Mycelial Growth ◽  
Inhibitory Effect ◽  
Heracleum Sosnowskyi ◽  
Half Maximal Effective Concentration ◽  
Inhibitory Compound ◽  
Race 1 ◽  
Race 2

The antifungal activity of volatile compounds from the fruit, leaf, rhizome and root of 109 plant species was evaluated against Fusarium oxysporum f. sp. lycopersici (FOL) race 1—the tomato wilt pathogen—by using the modified dish pack method. Eighty-eight plant samples inhibited mycelial growth, including volatiles from fruits of Heracleum sosnowskyi, which exhibited the strongest antifungal activity, showing 67% inhibition. Two volatile compounds from the fruits of H. sosnowskyi (octanol and octanal) and trans-2-hexenal as a control were tested for their antifungal activities against FOL race 1 and race 2. In terms of half-maximal effective concentration (EC50) values, octanol was found to be the most inhibitory compound for both pathogenic races, with the smallest EC50 values of 8.1 and 9.3 ng/mL for race 1 and race 2, respectively. In the biofumigation experiment, the lowest disease severity of tomato plants and smallest conidial population of race 1 and race 2 were found in trans-2-hexenal and octanol treated soil, while octanal had an inhibitory effect only on race 2. Therefore, our study demonstrated the effectiveness of volatile octanol and trans-2-hexenal on the control of the mycelial growth of two races of Fusarium oxysporum f. sp. lycopersici and may have potential for the future development of novel biofumigants.

Surface-modified sulfur nanoparticles: an effective antifungal agent against Aspergillus niger and Fusarium oxysporum

2011 ◽  
Vol 90 (2) ◽  
pp. 733-743 ◽  
Author(s):  
Samrat Roy Choudhury ◽  
Mahua Ghosh ◽  
Amrita Mandal ◽  
Dipankar Chakravorty ◽  
Moumita Pal ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Fusarium Oxysporum ◽  
Antifungal Agent ◽  
Surface Modified ◽  
Sulfur Nanoparticles

scholarly journals IDENTIFIKASI STRUKTUR SENYAWA ANTIJAMUR DARI RUMPUT LAUT

2014 ◽  
Vol 2 (1) ◽  
pp. 27
Author(s):  
Rahmawati Rahmawati
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Benzene Ring ◽  
Aspergillus Flavus ◽  
Sclerotium Rolfsii ◽  
Antifungal Compounds ◽  
Active Compounds ◽  
Activity Test ◽  
Antifungal Effects ◽  
Benzene Extract

Screening of plants antifungal compounds from Algae has been resulted. Screening was preceded by extraction of its fresh substance with benzene, methanol, and chloroform solvents and followed examination towards fungi Fusarium oxysforum, Sclerotium rolfsii, Aspergillus flavus, and Pythium spp. The antifungal effects were tested by well methode in potato dextrosa agar (PDA) medium. Analysis compound of benzene extract with preparative TLC methode (n-hexene : ethyl acetate : methanol = 7 : 2 : 1) obtained 5 compounds. Antifungal activity test of the fractions was done by the disk diffution methode with PDA medium. Identification of active compounds with spectroscophy method (IR and NMR) indicated the benzene extract analogue with fungicide Metalaxyl with the benzene ring and substituens of ester, alkyl, and amide. Identification of fractions showed that the compounds analogue with fungicides belong to carboxamide with the main substituen of benzene ring and ester/aldehids formed, alkyl, and  amide.

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