Cultivar-Specific Oxidative Modification of Proteins, Proteolytic Activity and Alterations in Proteomes of Rice Seedlings to Simultaneous Water Deficit and Aluminum Toxicity

Author(s):  
Poonam Pandey ◽  
Rama Shanker Dubey
2020 ◽  
Vol 33 (1) ◽  
pp. 38-44
Author(s):  
Alona Yurchenko ◽  
Daryna Krenytska ◽  
Olexii Savchuk ◽  
Tetiana Halenova ◽  
Natalia Raksha ◽  
...  

AbstractOur interest has focused on the investigation of the anti-obese potential of kidney beans (P. vulgaris) pods extract. In the course of the study, obesity development in rats was induced with high-calorie diet. Control and obese rats then have consumed with aqueous kidney beans (P. vulgaris) pods extract during 6 weeks (200 mg/kg). Results show that the long-term consumption of P. vulgaris pods extract can lead to the reduction of hyperglycemia and insulin resistance development. Furthermore, we saw a normalization of lipid peroxidation parameters and oxidative modification of protein due to the consumption of the kidney beans (P. vulgaris) pods extract. Our experimental data demonstrate the ability of the kidney beans (P. vulgaris) pod extracts to mitigate obesity development but the details of this mechanism remains to be not fully understood.


Author(s):  
V. B. Stetsevyat ◽  
N. M. Voronych-Semchenko

The results of the study about the nature of oxygen-dependent processes in rats that were on a high-fructose diet for 8 weeks under conditions of adequate iodine supply and congenital iodine deprivation is presented in the article. The significant activation of peroxide oxidation of proteins (by increasing the aldo- and keto-derivates of a neutral nature) and lipids (increased of diene conjugates content and products, that are responsible to thiobarbituric acid) of animals with insulin resistance was found. An aggravating factor in the course of these processes is a congenital iodine deficiency. The changes of oxidative modification of proteins in animals with congenital iodine deficiency had multidirectional character, and processes of lipid peroxidation mainly increased. The most pronounced changes of the studied processes were observed in the liver and pancreas of animals with insulin resistance against the background of congenital iodine deficiency.


2006 ◽  
Vol 27 (1) ◽  
pp. 42-53 ◽  
Author(s):  
Minna A. Korolainen ◽  
Gundars Goldsteins ◽  
Tuula A. Nyman ◽  
Irina Alafuzoff ◽  
Jari Koistinaho ◽  
...  

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Sahoko Ichihara ◽  
Yuka Suzuki ◽  
Jie Chang ◽  
Kentaro Kuzuya ◽  
Chisa Inoue ◽  
...  

2016 ◽  
Vol 96 ◽  
pp. S68
Author(s):  
Elisabetta Chiaradia ◽  
Fiorella D'Onofrio ◽  
Angela Gambelunghe ◽  
Lorena Urbanelli ◽  
Carla Emiliani ◽  
...  

2019 ◽  
Vol 5 (3) ◽  
pp. 28-32
Author(s):  
I. S. Edelev ◽  
L. M. Obukhova ◽  
N. A. Andriyanova ◽  
N. S. Edelev

In the article we present results of the studies in cases of death with a long agonal period to determine the length of the agony.Objectives. The task was to examine the features of oxidative modification of serum proteins in cases of deaths with a long agonal period.Material and methods. In all experiments we analyzed the liquid blood sampled from 54 corpses of persons of both sexes who died from the violent and natural death in age of 13 to 89 years. The assessment of the oxidative modification of proteins (OMP) was performed by the level of carbonyl derivatives detected in the reaction with 2,4-dinitrophenylhydrazine. The levels of absolute and relative (in terms of protein) total OMP in cases of long agonal period were compared with the counterpart levels in cases of the short agony. The duration of the agonу was determined using information about the circumstances of the case and investigative data with the permission of the authority ordered the forensic examination. The autopsy data also were taken into account.Results. We revealed the dependence of the OMP level on the duration of the agonal period. The total OMP (in terms of protein) in cases of long agony have decreased by 45.46 %. The data obtained have showed that the parameters of the OMP, taking into account all the relevant factors, can be used to develop criteria for establishing the duration of the agonal period as well as to increase the evidence-based significance of medico-legal conclusions.


Author(s):  
M. I. Marushchak ◽  
O. M. Kopanitsa ◽  
I. Ya. Krynytska ◽  
T. Ya. Yaroshenko

Introduction. In the last decades, the carrageenans have become one of the most popular hydrocolloids in the food industry. In addition to the positive properties of hydrocolloids, researchers have established the relationship between the incidence of ulcerative colitis and the level of carrageenan consumption, which makes it considered as a potential etiological factor in the pathology of the gastrointestinal tract.The aim of the study – to determine the level of oxidative modification of proteins of the wall of the small intestine, tissues of the heart and liver of rats using 1% κ-carrageenan solution.Materials and Methods. The study was carried out on 24 white non-linear male rats. The animals of the experimental group were provided with free access to a 1.0% of carrageenan solution for 1 month. In the selected samples of the small intestine, heart and liver was evaluated the peroxidic oxidation of proteins according to the level of their oxidation modifications products using spectrophotometry at wavelengths of 370 and 430 nm.Research Results. A significant increasing of ketone dinitrophenylhydrazones levels in the small intestine wall (by 57.0%) and in the liver (by 23.0%) and, respectively, the aldehyde dinitrophenylhydrazones levels by 47.7% and 19.1%, compared with the control values (p<0.01). In this case, the peroxide oxidation, to a greater extent, was subjected to neutral proteins. It should be noted that the peroxidic oxidation of proteins in the myocardium of the rats with carrageenan consumption, recorded at a wavelength of 370 nm, exceeded the control data by 14.3% (p<0.05).Conclusions. Oral administration of a 1% carrageenan solution in experimental animals leads to statistically significant activation statistically significant activation of peroxidation of proteins in the wall of the small intestine and the liver, whereas only the growth of neutral ketone dinitrophenylhydrazones is registered in the myocardium (p<0.05).


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