Analysis of DNA Polymerase λ Activity and Gene Expression in Response to Salt and Drought Stress in Oryza sativa Indica Rice Cultivars

Abstract Germin-like proteins (GLPs) are ubiquitous plant proteins, which play significant role in plant responses against various abiotic stresses. However, the potential functions of GLPs in rice (Oryza Sativa) against salt and drought stress are still unclear. In this study, transcriptional variation of 8 OsGLP genes (OsGLP3-6, OsGLP4-1, OsGLP8-4, OsGLP8-7, OsGLP8-10, OsGLP8-11 and OsGLP8-12) was analyzed in leaves and roots of two economically important Indica rice cultivars, KS282 and Super Basmati under salt and drought stress at early seedling stage. The relative expression analysis from qRT-PCR indicated the highest increase in expression of OsGLP3-6 in leaves and roots of both rice varieties with a significantly higher expression in KS282. Moreover, relative change in expression of OsGLP8-7, OsGLP8-10 and OsGLP8-11 under salt stress and OsGLP8-7 under drought stress was also commonly higher in leaves and roots of KS282 as compared to Super Basmati. Whereas, OsGLP3-7 and OsGLP8-12 after salt stress and OsGLP8-4 and OsGLP8-12 after drought stress were observed with higher relative expression in roots of Super Basmati than KS282. Importantly, the OsGLP3-6 and OsGLP4-1 from chromosome 3 and 4 respectively showed higher expression in leaves whereas most of the OsGLP genes from chromosome 8 exhibited higher expression in roots. Overall, as a result of this comparative analysis, OsGLP genes showed both general and specific expression profiles depending upon a specific rice variety, stress condition as well as tissue type. These results will increase our understanding of role of OsGLP genes in rice crop and provide useful information for the further in-depth research on their regulatory mechanisms in response to these stress conditions.

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Germin like protein genes exhibit modular expression during salt and drought stress in elite rice cultivars

Molecular Biology Reports ◽

10.1007/s11033-021-06871-3 ◽

2021 ◽

Author(s):

Jazba Anum ◽

Charlotte O’Shea ◽

M. Zeeshan Hyder ◽

Sumaira Farrukh ◽

Karen Skriver ◽

...

Keyword(s):

Drought Stress ◽

Rice Cultivars ◽

Salt And Drought Stress

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Gene Expression Profiles Deciphering the Pathways of Coronatine Alleviating Water Stress in Rice (Oryza sativa L.) Cultivar Nipponbare (Japonica)

International Journal of Molecular Sciences ◽

10.3390/ijms20102543 ◽

2019 ◽

Vol 20 (10) ◽

pp. 2543 ◽

Cited By ~ 5

Author(s):

Wei Gao ◽

Chunxin Yu ◽

Lin Ai ◽

Yuyi Zhou ◽

Liusheng Duan

Keyword(s):

Gene Expression ◽

Oryza Sativa ◽

Water Stress ◽

Drought Stress ◽

Oryza Sativa L ◽

Expression Profiles ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Gene Expression Profiles ◽

Rice Seedling

Coronatine (COR) is a structural and functional analog of methyl jasmonic acid (MeJA), which can alleviate stress on plant. We studied the effects of COR on the drought stress of rice (Oryza sativa L.). Pre-treatment with COR significantly increased the biomass, relative water and proline content, and DPPH (1,1-diphenyl-2-picrylhydrazyl)-radical scavenging activity, decreased the electrolyte leakage and MDA (Malondialdehyde) content in order to maintain the stability of cell membrane. Meanwhile, we determined how COR alleviates water stress by Nipponbare gene expression profiles and cDNA microarray analyses. Seedlings were treated with 0.1 μmol L−1 COR at the three leafed stage for 12 h, followed with 17.5% polyethylene glycol (PEG). Whole genome transcript analysis was determined by employing the Rice Gene Chip (Affymetrix), a total of 870 probe sets were identified to be up or downregulated due to COR treatment under drought stress. Meanwhile, the real-time quantitative PCR (RT-qPCR) method was used to verify some genes; it indicated that there was a good agreement between the microarray data and RT-qPCR results. Our data showed that the differentially expressed genes were involved in stress response, signal transduction, metabolism and tissue structure development. Some important genes response to stress were induced by COR, which may enhance the expression of functional genes implicated in many kinds of metabolism, and play a role in defense response of rice seedling to drought stress. This study will aid in the analysis of the expressed gene induced by COR.

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Rare-earth element scandium improves stomatal regulation and enhances salt and drought stress tolerance by up-regulating antioxidant responses of Oryza sativa

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2020.04.040 ◽

2020 ◽

Vol 152 ◽

pp. 157-169 ◽

Cited By ~ 2

Author(s):

Fevzi Elbasan ◽

Ceyda Ozfidan-Konakci ◽

Evren Yildiztugay ◽

Mustafa Kucukoduk

Keyword(s):

Oryza Sativa ◽

Rare Earth ◽

Drought Stress ◽

Stress Tolerance ◽

Rare Earth Element ◽

Earth Element ◽

Antioxidant Responses ◽

Drought Stress Tolerance ◽

Stomatal Regulation ◽

Salt And Drought Stress

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Exogenous ABA induces salt tolerance in indica rice (Oryza sativa L.): The role of OsP5CS1 and OsP5CR gene expression during salt stress

Environmental and Experimental Botany ◽

10.1016/j.envexpbot.2010.01.009 ◽

2013 ◽

Vol 86 ◽

pp. 94-105 ◽

Cited By ~ 72

Author(s):

Siriporn Sripinyowanich ◽

Pongsathorn Klomsakul ◽

Bongkoj Boonburapong ◽

Thapana Bangyeekhun ◽

Tadao Asami ◽

...

Keyword(s):

Gene Expression ◽

Oryza Sativa ◽

Salt Stress ◽

Salt Tolerance ◽

Oryza Sativa L ◽

Indica Rice ◽

Exogenous Aba

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Directional selection at gene expression level contributes to the speciation of Asian rice cultivars

10.1101/2021.10.31.466501 ◽

2021 ◽

Author(s):

Lihong Xie ◽

Kehan Yu ◽

Dongjing Chen

Keyword(s):

Gene Expression ◽

Oryza Sativa ◽

Oryza Sativa L ◽

Directional Selection ◽

Expression Level ◽

Stabilizing Selection ◽

Rice Cultivars ◽

Gene Expression Variation ◽

Expression Variation ◽

Asian Rice

Differences in expression levels play important roles in phenotypic variation across species, especially those closely related species with limited genomic differences. Therefore, studying gene evolution at expression level is important for illustrating phenotypic differentiation between species, such as the two Asian rice cultivars, Oryza sativa L. ssp. indica and Oryza sativa L. ssp. japonica. In this study, we evaluated the gene expression variation at inter-subspecies and intra-subspecies levels using transcriptome data from seedlings of three indica and japonica rice and defined four groups of genes under different natural selections. We found a substantial of genes (about 79%) that are under stabilizing selection at the expression level in both subspecies, while about 16% of genes are under directional selection. Genes under directional selection have higher expression level and lower expression variation than those under stabilizing selection, which suggest a potential explanation to subspecies adaptation to different environments and interspecific phenotypic differences. Subsequent functional enrichment analysis of genes under directional selection shows that indica rice have experienced the adaptation to environmental stresses, and also show differences in biosynthesis and metabolism pathways. Our study provides an avenue of investigating indica-japonica differentiation through gene expression variation, which may guide to rice breeding and yield improvement.

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Gene Expression in Leaves and Epidermis of Arabidopsis under Salt and Drought Stress

South Asian Research Journal of Biology and Applied Biosciences ◽

10.36346/sarjbab.2019.v01i01.005 ◽

2019 ◽

Vol 01 (01) ◽

pp. 30-32

Author(s):

Khalifa Suleiman Mohamed

Keyword(s):

Gene Expression ◽

Drought Stress ◽

Salt And Drought Stress

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Gene Expression Analysis of Resistant and Susceptible Rice Cultivars to Sheath Blight after Inoculation with Rhizoctonia Solani

10.21203/rs.3.rs-761234/v1 ◽

2021 ◽

Author(s):

Xiaohe Yang ◽

Xin Gu ◽

Junjie Ding ◽

Liangliang Yao ◽

Xuedong Gao ◽

...

Keyword(s):

Gene Expression ◽

Rhizoctonia Solani ◽

Metabolic Pathways ◽

Indica Rice ◽

Leaf Sheath ◽

Sheath Blight ◽

Japonica Rice ◽

Rice Cultivars ◽

Rice Sheath Blight ◽

Go Annotation

Abstract Background: Rice sheath blight, caused by Rhizoctonia solani Kühn (teleomorph: Thanatephorus cucumeris), is one of the most severe diseases in rice (Oryza sativa L.) worldwide. Studies on resistance genes and resistance mechanisms of rice sheath blight has mainly focused on indica rice. Rice sheath blight is a growing threat to rice production with the increasing planting area of japonica rice in Northeast China, and it is therefore essential to explore the mechanism of sheath blight resistance in this rice subspecies.Results: In this study, RNA-seq technology was used to analyse the gene expression changes of leaf sheath inoculation with R. solani at 12, 24, 36, 48, and 72 h after inoculation of the resistant cultivar ‘Shennong 9819’ and susceptible cultivar ‘Yueguang’. A total of 2275 differentially expressed genes (DEGs) were identified in our study. Gene Ontology (GO) annotation and Kyoto Encyclopaedia of Genes and Genomes (KEGG) enrichment analysis of these DEGs showed that the metabolic pathways of the two rice cultivars were similar and approximately covered most of the biological processes after inoculation with R. solani. Conclusion: The main differences between the resistant and susceptible cultivars were the activity of DEGs in response to R. solani and the enrichment of metabolic pathways involved in these genes. Upon experiencing external stress, the genes in the leaf sheath of Shennong 9819 showed a fluctuating response, whereas the genes in the leaf sheath of Yueguang had a higher activity. The phenylalanine metabolism and plant hormone signal transduction play important roles in disease resistance. Their response in the leaf sheath of Shennong 9819 was faster than that of Yueguang, and they were highly enriched at 12 h after inoculation.

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