Germin like protein genes exhibit modular expression during salt and drought stress in elite rice cultivars

Abstract Germin-like proteins (GLPs) are ubiquitous plant proteins, which play significant role in plant responses against various abiotic stresses. However, the potential functions of GLPs in rice (Oryza Sativa) against salt and drought stress are still unclear. In this study, transcriptional variation of 8 OsGLP genes (OsGLP3-6, OsGLP4-1, OsGLP8-4, OsGLP8-7, OsGLP8-10, OsGLP8-11 and OsGLP8-12) was analyzed in leaves and roots of two economically important Indica rice cultivars, KS282 and Super Basmati under salt and drought stress at early seedling stage. The relative expression analysis from qRT-PCR indicated the highest increase in expression of OsGLP3-6 in leaves and roots of both rice varieties with a significantly higher expression in KS282. Moreover, relative change in expression of OsGLP8-7, OsGLP8-10 and OsGLP8-11 under salt stress and OsGLP8-7 under drought stress was also commonly higher in leaves and roots of KS282 as compared to Super Basmati. Whereas, OsGLP3-7 and OsGLP8-12 after salt stress and OsGLP8-4 and OsGLP8-12 after drought stress were observed with higher relative expression in roots of Super Basmati than KS282. Importantly, the OsGLP3-6 and OsGLP4-1 from chromosome 3 and 4 respectively showed higher expression in leaves whereas most of the OsGLP genes from chromosome 8 exhibited higher expression in roots. Overall, as a result of this comparative analysis, OsGLP genes showed both general and specific expression profiles depending upon a specific rice variety, stress condition as well as tissue type. These results will increase our understanding of role of OsGLP genes in rice crop and provide useful information for the further in-depth research on their regulatory mechanisms in response to these stress conditions.

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Analysis of DNA Polymerase λ Activity and Gene Expression in Response to Salt and Drought Stress in Oryza sativa Indica Rice Cultivars

Journal of Plant Growth Regulation ◽

10.1007/s00344-021-10390-7 ◽

2021 ◽

Author(s):

Sayantani Sihi ◽

Sankar Bakshi ◽

Soumitra Maiti ◽

Arup Nayak ◽

Dibyendu Narayan Sengupta

Keyword(s):

Gene Expression ◽

Oryza Sativa ◽

Drought Stress ◽

Dna Polymerase ◽

Indica Rice ◽

Rice Cultivars ◽

Salt And Drought Stress ◽

Dna Polymerase Λ

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Molecular and Physiological Responses of Rice and Weedy Rice to Heat and Drought Stress

Agriculture ◽

10.3390/agriculture11010009 ◽

2020 ◽

Vol 11 (1) ◽

pp. 9

Author(s):

Leonard Bonilha Piveta ◽

Nilda Roma-Burgos ◽

José Alberto Noldin ◽

Vívian Ebeling Viana ◽

Claudia de Oliveira ◽

...

Keyword(s):

Genetic Diversity ◽

Heat Stress ◽

Drought Stress ◽

Intercellular Co2 Concentration ◽

Tiller Number ◽

Weedy Rice ◽

Photosynthetic Parameters ◽

World Population ◽

Rice Grain ◽

Rice Cultivars

Rice is the staple food for about half of the world population. Rice grain yield and quality are affected by climatic changes. Arguably, rice cultivars’ genetic diversity is diminished from decades of breeding using narrow germplasm, requiring introgressions from other Oryza species, weedy or wild. Weedy rice has high genetic diversity, which is an essential resource for rice crop improvement. Here, we analyzed the phenotypic, physiological, and molecular profiles of two rice cultivars (IRGA 424 and SCS119 Rubi) and five weedy rice (WR), from five different Brazilian regions, in response to heat and drought stress. Drought and heat stress affected the phenotype and photosynthetic parameters in different ways in rice and WR genotypes. A WR from Northern Brazil yielded better under heat stress than the non-stressed check. Drought stress upregulated HSF7A while heat stress upregulated HSF2a. HSP74.8, HSP80.2, and HSP24.1 were upregulated in both conditions. Based on all evaluated traits, we hypothesized that in drought conditions increasing HSFA7 expression is related to tiller number and that increase WUE (water use efficiency) and HSFA2a expression are associated with yield. In heat conditions, Gs (stomatal conductance) and E’s increases may be related to plant height; tiller number is inversely associated with HSPs expression, and chlorophyll content and Ci (intercellular CO2 concentration) may be related to yield. Based on morphology, physiology, and gene regulation in heat and drought stress, we can discriminate genotypes that perform well under these stress conditions and utilize such genotypes as a source of genetic diversity for rice breeding.

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Role of Kinases for Regulating K + Homeostasis Under Salt and Drought Stress Conditions

Protein Kinases and Stress Signaling in Plants ◽

10.1002/9781119541578.ch21 ◽

2020 ◽

pp. 479-500

Author(s):

Jayant Kulkarni ◽

Ashish K. Srivastava ◽

Suprasanna Penna

Keyword(s):

Drought Stress ◽

Stress Conditions ◽

Salt And Drought Stress

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Proteome Analysis for Understanding Abiotic Stress (Salinity and Drought) Tolerance in Date Palm (Phoenix dactyliferaL.)

International Journal of Genomics ◽

10.1155/2015/407165 ◽

2015 ◽

Vol 2015 ◽

pp. 1-11 ◽

Cited By ~ 14

Author(s):

Haddad A. El Rabey ◽

Abdulrahman L. Al-Malki ◽

Khalid O. Abulnaja ◽

Wolfgang Rohde

Keyword(s):

Drought Stress ◽

Chlorophyll A ◽

Date Palm ◽

Light Harvesting ◽

Binding Protein ◽

Proteome Analysis ◽

Rubisco Activase ◽

Stress Conditions ◽

Spectrometric Analysis ◽

Salt And Drought Stress

This study was carried out to study the proteome of date palm under salinity and drought stress conditions to possibly identify proteins involved in stress tolerance. For this purpose, three-month-old seedlings of date palm cultivar “Sagie” were subjected to drought (27.5 g/L polyethylene glycol 6000) and salinity stress conditions (16 g/L NaCl) for one month. DIGE analysis of protein extracts identified 47 differentially expressed proteins in leaves of salt- and drought-treated palm seedlings. Mass spectrometric analysis identified 12 proteins; three out of them were significantly changed under both salt and drought stress, while the other nine were significantly changed only in salt-stressed plants. The levels of ATP synthase alpha and beta subunits, an unknown protein and some of RubisCO fragments were significantly changed under both salt and drought stress conditions. Changes in abundance of superoxide dismutase, chlorophyll A-B binding protein, light-harvesting complex1 protein Lhca1, RubisCO activase, phosphoglycerate kinase, chloroplast light-harvesting chlorophyll a/b-binding protein, phosphoribulokinase, transketolase, RubisCO, and some of RubisCO fragments were significant only for salt stress.

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