scholarly journals Bactericidal activity of three different antiseptic ophthalmic preparations as surgical prophylaxis

2021 ◽  
Author(s):  
Daniele Tognetto ◽  
Marco R. Pastore ◽  
Gian Marco Guerin ◽  
Giuliana Decorti ◽  
Martina Franzin ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Povidone Iodine ◽  
Bacterial Load ◽  
Viable Cells ◽  
Polyethylene Glycol 1000 ◽  
Oil Concentration ◽  
Short Time ◽  
First Time ◽  
Antiseptic Solutions

Abstract Purpose In the era of antibiotic resistance, there is an increased interest in antiseptic solutions that might represent a reliable option for ocular surface disinfection. The objective of this study is to compare for the first time three different antiseptic ophthalmic preparations to assess their in vitro antimicrobial activity. Methods The antiseptic activity of three commercial ophthalmic solutions, IODIM (povidone-iodine 0.6% in hyaluronic acid vehicle—Medivis, Catania, Italy), OZODROP (nanoemulsion with ozonated oil—concentration not specified—FBVision, Ophthalmic Pharmaceuticals, Rome, Italy), and DROPSEPT (chlorhexidine 0.02% and vitamin E 0.5% Tocopherol Polyethylene Glycol 1000 Succinate—TPGS, Sooft Italia, Montegiorgio, Italy), was tested in vitro on six reference strains by time-killing assays. Viable cells were evaluated after 1, 15, 30 min; 2, 6, and 24 h exposure by seeding 100 µl of the suspension (or appropriate dilutions) on LB agar or Sabouraud-dextrose agar. All plates were incubated at 37 °C for 24 h and evaluated by manually counting the colonies. Results IODIM solution showed a very rapid microbicidal activity: the number of viable cells for all the tested strains was under the detection limit (less than 10 CFU/ml) already after 1 min exposure, and this result was maintained at every incubation time. The rapid antimicrobial activity of povidone-iodine was not replicated when testing the other two antiseptics. Conclusions The study reports the great efficacy in reducing bacterial load in a very short time of povidone-iodine 0.6% compared with other antiseptic preparations.

scholarly journals Detection ofC. trachomatisin the Serum of the Patients with Urogenital Chlamydiosis

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Naylia A. Zigangirova ◽  
Yulia P. Rumyantseva ◽  
Elena Y. Morgunova ◽  
Lidia N. Kapotina ◽  
Lubov V. Didenko ◽  
...  
Keyword(s):  
Bacterial Load ◽  
Cell Monolayer ◽  
Immunogold Electron Microscopy ◽  
Rna Detection ◽  
Systemic Spread ◽  
Anatomical Localization ◽  
Taqman Pcr ◽  
Spread Of Infection ◽  
First Time

Extragenital chlamydial complications may be associated with systemic spread of infection, but haematogenous route forC. trachomatisdissemination has not been clearly demonstrated. Here we report that serum specimens obtained from patients with chlamydiosis contain elementary bodies ofC. trachomatisshown by culture and immunogold electron microscopy. We have found that 31 of the 52 patients had serum precipitates which were infective to McCoy cells. Immunostaining revealed very small inclusions resembling those reported during persistentC. trachomatisinfectionin vitro. DNA specimens from 49 (out of 52) patients with chlamydiosis gave positive PCR readings. The viability of the pathogen present in the sera was confirmed by chlamydial RNA detection in the cell monolayer inoculated by the serum precipitates. By using DNA isolation protocol from 1 mL of serum and quantitative TaqMan PCR, it was estimated that bacterial load in patients’ sera was2×102–103 GE/mL. These findings for the first time demonstrated thatC. trachomatiscan be disseminated directly by the plasma, independently from blood cell, which may represent a new possible pathway of the chronic infection development. Therefore, new methodological approaches for detection ofC. trachomatisin the serum of patients with complicated and chronic chlamydiosis could be important in the diagnosis of the infection regardless of its anatomical localization.

scholarly journals Nitrogen and Bromide Co-Doped Hydroxyapatite Thin Films with Antimicrobial Properties

Coatings ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1505
Author(s):  
Simona Liliana Iconaru ◽  
Carmen Steluta Ciobanu ◽  
Daniela Predoi ◽  
Mikael Motelica-Heino ◽  
Constantin Cătălin Negrilă ◽  
...  
Keyword(s):  
Thin Films ◽  
Antimicrobial Activity ◽  
Spin Coating ◽  
Thin Layers ◽  
Precipitation Method ◽  
X Ray ◽  
Co Precipitation ◽  
First Time ◽  
Co Doped

Hydroxyapatite (Ca10(PO4)6(OH)2, HAp), due to its high biocompatibility, is widely used as biomaterial. Doping with various ions of hydroxyapatite is performed to acquire properties as close as possible to the biological apatite present in bones and teeth. In this research the results of a study performed on thin films of hydroxyapatite co-doped with nitrogen and bromine (NBrHAp) are presented for the first time. The NBrHAp suspension was obtained by performing the adapted co-precipitation method using cetyltrimethylammonium bromide (CTAB). The thin layers of NBrHAp were obtained by spin-coating. The stability of the NBrHAp suspension was examined by ultrasound measurements. The thin layers obtained by the spin-coating method were examined by scanning electron microscopy (SEM), optical microscopy (OM), and metallographic microscopy (MM). The presence of nitrogen and bromine were highlighted by energy-dispersive X-ray spectroscopy (EDS) and X-ray photoelectron spectroscopy (XPS) studies. Fourier transform infrared spectroscopy (FTIR) was used to highlight the chemical status of nitrogen and bromine. In addition, the powder obtained from the NBrHAp suspension was analyzed by XRD. Moreover, the in vitro antimicrobial activity of the NBrHAp suspensions and coatings was investigated using the reference microbial strains Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Candida albicans ATCC 10231. The results highlighted the successful obtainment of N and Br co-doped hydroxyapatite suspension for the first time by an adapted co-precipitation method. The obtained suspension was used to produce pure NBrHAp composite thin films with superior morphological properties. The NBrHAp suspensions and coatings exhibited in vitro antimicrobial activity against bacterial and fungal strains and revealed their good antimicrobial activity.

scholarly journals New Poly(Propylene Imine) Dendrimer Modified with Acridine and Its Cu(II) Complex: Synthesis, Characterization and Antimicrobial Activity

Materials ◽  
2019 ◽  
Vol 12 (18) ◽  
pp. 3020 ◽  
Author(s):  
Paula Bosch ◽  
Desislava Staneva ◽  
Evgenia Vasileva-Tonkova ◽  
Petar Grozdanov ◽  
Ivanka Nikolova ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Copper Ions ◽  
Spectral Characteristics ◽  
Dilution Method ◽  
Pathogenic Microorganisms ◽  
Paramagnetic Resonance ◽  
Poly Propylene ◽  
New Compounds ◽  
First Time

A second-generation poly(propylene imine) dendrimer modified with acridine and its Cu(II) complex have been synthesized for the first time. It has been found that two copper ions form complexes with the nitrogen atoms of the dendrimeric core by coordinate bonds. The new compounds have been characterized by nuclear magnetic resonance (NMR), electron paramagnetic resonance (EPR), fourier-transform infrared spectroscopy (FTIR) and fluorescence spectroscopy. The spectral characteristics of the modified dendrimer have been measured in different organic solvents, and a negative fluorescence solvatochromism has been observed. The antimicrobial activity of the dendrimers has been tested against model pathogenic microorganisms in agar and by broth dilution method. The cotton fabric treated with both dendrimers has been evaluated towards pathogenic microorganisms. The obtained modified cotton fabrics have been shown to hamper bacterial growth and to prevent biofilm formation. Dendrimer cytotoxicity has been investigated in vitro in the model HEp-2 cell line.

In vitro antimicrobial activity of a new ophthalmic solution containing povidone‐iodine 0.6% ( IODIM ® )

2019 ◽  
Vol 98 (2) ◽  
Author(s):  
Antonio Pinna ◽  
Matthew Gavino Donadu ◽  
Donatella Usai ◽  
Stefano Dore ◽  
Giuseppe D'Amico‐Ricci ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Povidone Iodine ◽  
Ophthalmic Solution ◽  
In Vitro Antimicrobial Activity

In vitro Short-Time Killing Activity of Povidone-Iodine (Isodine® Gargle) in the Presence of Oral Organic Matter

Dermatology ◽  
2006 ◽  
Vol 212 (1) ◽  
pp. 103-108 ◽  
Author(s):  
Akiko Yoneyama ◽  
Masaki Shimizu ◽  
Makiko Tabata ◽  
Junko Yashiro ◽  
Toshihiko Takata ◽  
...  
Keyword(s):  
Organic Matter ◽  
Povidone Iodine ◽  
Killing Activity ◽  
Short Time

scholarly journals In vivo antimicrobial activity of 0.6% povidone-iodine eye drops in patients undergoing intravitreal injections: a prospective study

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Daniele Tognetto ◽  
Marco R. Pastore ◽  
Lorenzo Belfanti ◽  
Riccardo Merli ◽  
Alex L. Vinciguerra ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Povidone Iodine ◽  
Culture Media ◽  
Bacterial Load ◽  
Cohort Analysis ◽  
Control Group ◽  
Eye Drops ◽  
Coagulase Negative Staphylococci ◽  
Intravitreal Treatment ◽  
Preservative Free

AbstractTo investigate the antimicrobial activity of a preservative-free 0.6% povidone-iodine eye drop as an antiseptic procedure in decreasing the conjunctival bacterial load in eyes scheduled for intravitreal treatment and to compare its efficacy to the untreated fellow eye used as the control group. Prospective cohort analysis in which 208 patients received preservative-free 0.6% povidone-iodine eye drops three times a day for three days before intravitreal injection. Before and after the prophylactic treatment, a conjunctival swab was collected from both the study eye and the untreated contralateral eye, used as control. The swab was inoculated on different culture media and the colony-forming units were counted. Bacteria and fungi were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Treatment with 0.6% povidone-iodine eye drops significantly reduced the conjunctival bacterial load from baseline (p < 0.001 for blood agar and p < 0.001 for chocolate agar) with an eradication rate of 80%. The most commonly isolated pathogen at each time-point and in both groups was coagulase-negative Staphylococci, isolated in 84% of the positive cultures. The study provides evidence about the effectiveness of 0.6% povidone-iodine eye drops treatment in reducing the conjunctival bacterial load in eyes scheduled for intravitreal treatment.

scholarly journals Microwave assisted the short time clean synthesis of 1,3-Diketones as Building Blocks in Heterocyclic Synthesis: A Facile Synthesis and Antimicrobial Evaluation of New Dihydropyridine, 4H-Pyrane, Dihydropyridazine, Pyrimidine and Pyrazole derivatives

2019 ◽  
Author(s):  
mohamed ahmed abdelreheim ◽  
Ibrahim Saad Abdel Hafiz ◽  
Hend Saad Eldin Abdel Rady
Keyword(s):  
Antimicrobial Activity ◽  
Wide Spectrum ◽  
Building Blocks ◽  
Mycelial Fungi ◽  
Mass Spectral ◽  
H Nmr ◽  
Chemical Structures ◽  
Short Time ◽  
Anti Fungal

Abstract Background: According to literature survey, the compounds bearing naphthalene moiety can be used as medical preparations because of their wide spectrum of biological activity and low toxicity. In this study, a new series of azoles or azines were synthesized from the reaction of the key intermediate 1-(1-hydroxynaphthalen-2-yl)-3-phenylpropane-1,3-dione 3 with a variety of electrophilic and nucleophilic reagents under a variety of mild conditions. Results: The chemical structures of these compounds were confirmed by various spectroscopic methods such as (IR, 1H-NMR, 13C-NMR, mass spectral data and elemental analyses). Conclusions: The prepared compounds were screened in vitro for their antimicrobial activity against some species of Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and Gram-negative bacteria (Escherichia coli and Pseudomonas aeuroginosa). Anti-fungal activities of the compounds were tested against yeast and mycelial fungi,Candida albicans and Aspergillus flavus. The antimicrobial activity of this series was showed either weak or moderate activities.

scholarly journals A Novel Cd(II) Isophthalate Complex with Triethanolamine: Crystal Structure, Fluorescence and Antimicrobial Activity

2021 ◽  
Vol 68 (2) ◽  
pp. 466-474
Author(s):  
Zuhal Yolcu ◽  
Sinem Yurtcan ◽  
Meryem Çıtlakoğlu
Keyword(s):  
Antimicrobial Activity ◽  
Room Temperature ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Single Crystal Diffraction ◽  
X Ray ◽  
Ft Ir ◽  
First Time ◽  
Trigonal Prismatic

A mixed ligand Cd(II) complex [Cd(IsoPht)(TEA)H2O]·3H2O was synthesized for the first time by using isophthalic acid (H2IsoPht) and tetradentate triethanolamine (TEA) and characterized by X-ray single-crystal diffraction, FT-IR, and thermogravimetric analysis (TGA). This novel complex crystallizes in the triclinic system with P-1 space group and distorted monocapped trigonal prismatic geometry. The Cd(II) has seven coordinates with bidentate IsoPht, a TEA in the tetradentate mode, and an aqua ligand. The fluorescence properties of the Cd(II) complex and TEA ligand were investigated at room temperature. The present Cd(II) complex was also tested for its antimicrobial activity by in vitro agar diffusion method against some Gram-positive and Gram-negative bacteria and a fungus.

scholarly journals A Novel Qualitative and Quantitative Biofilm Assay Based on 3D Soft Tissue

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Bodil Hakonen ◽  
Linnea K. Lönnberg ◽  
Eva Larkö ◽  
Kristina Blom
Keyword(s):  
Antimicrobial Activity ◽  
Soft Tissues ◽  
Bacterial Load ◽  
Wound Dressings ◽  
Rapid Screening ◽  
Qualitative And Quantitative ◽  
Development Of Resistance ◽  
Novel Method

The lack of predictablein vitromethods to analyze antimicrobial activity could play a role in the development of resistance to antibiotics. Current used methods analyze planktonic cells but for the method to be clinically relevant, biofilm inin vivolike conditions ought to be studied. Hence, our group has developed a qualitative and quantitative method within vivolike 3D tissue for prediction of antimicrobial activity in reality. Devices (wound dressings) were applied on top ofPseudomonas aeruginosainoculated Muller-Hinton (MH) agar or 3D synthetic soft tissues (SST) and incubated for 24 hours. The antibacterial activity was then analyzed visually and by viable counts. On MH agar two out of three silver containing devices showed zone of inhibitions (ZOI) and on SST, ZOI were detected for all three. Corroborating results were found upon evaluating the bacterial load in SST and shown to be silver concentration dependent. In conclusion, a novel method was developed combining visual rapid screening and quantitative evaluation of the antimicrobial activity in both tissue and devices. It uses tissue allowing biofilm formation thus mimicking reality closely. These conditions are essential in order to predict antimicrobial activity of medical devices in the task to prevent device related infections.

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