Involvement of NAC transcription factor SiNAC1 in a positive feedback loop via ABA biosynthesis and leaf senescence in foxtail millet

Planta ◽  
2017 ◽  
Vol 247 (1) ◽  
pp. 53-68 ◽  
Author(s):  
Tingting Ren ◽  
Jiawei Wang ◽  
Mingming Zhao ◽  
Xiaoming Gong ◽  
Shuxia Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Leaf Senescence ◽  
Positive Feedback ◽  
Feedback Loop ◽  
Foxtail Millet ◽  
Nac Transcription Factor ◽  
Positive Feedback Loop ◽  
Aba Biosynthesis

scholarly journals ETS Transcription Factor ESE1/ELF3 Orchestrates a Positive Feedback Loop That Constitutively Activates NF-κB and Drives Prostate Cancer Progression

2013 ◽  
Vol 73 (14) ◽  
pp. 4533-4547 ◽  
Author(s):  
Nicole Longoni ◽  
Manuela Sarti ◽  
Domenico Albino ◽  
Gianluca Civenni ◽  
Anastasia Malek ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Transcription Factor ◽  
Cancer Progression ◽  
Positive Feedback ◽  
Feedback Loop ◽  
Prostate Cancer Progression ◽  
Positive Feedback Loop ◽  
Ets Transcription Factor

scholarly journals A Positive Feedback Loop Between Gastric Cancer Cells and Tumor-associated Macrophage Induces Malignancy Progression

2022 ◽  
Author(s):  
Haiyan Piao ◽  
Lingfeng Fu ◽  
Yang Liu ◽  
Yue Wang ◽  
Xiangyu Meng ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Transcription Factor ◽  
Cancer Cells ◽  
Positive Feedback ◽  
Feedback Loop ◽  
Nuclear Factor Kappa B ◽  
Luciferase Reporter ◽  
Nuclear Factor ◽  
Positive Feedback Loop ◽  
Nuclear Factor Kappa

Abstract Background: Hypoxia and inflammation tumor microenvironment (TME) play a crucial role in tumor development and progression. Although increased understanding of TME contributed to gastric cancer (GC) progression and prognosis, the direct interaction between macrophage and GC cells was not fully understood.Methods: Hypoxia and normoxia macrophage microarrays of GEO database was analyzed. The peripheral blood mononuclear cell acquired from the healthy volunteers. The expression of CXCL8 in GC tissues and cell lines was detected by quantitative reverse transcription PCR (qRT-PCR), western-blot, Elisa and immunofluorescence. Cell proliferation, migration, and invasion were evaluated by cell counting kit 8 (CCK8), colony formation, real-time imaging of cell migration and transwell. Luciferase reporter assays and chromatin immunoprecipitation were used to identify the interaction between transcription factor and target gene. Especially, a series of truncated and mutation reporter genes were applied to identify precise binding sites.The corresponding functions were verified in the complementation test and in vivo animal experiment.Results: Our results revealed that Hypoxia triggered macrophage secreted C-X-C Motif Chemokine Ligand 8 (CXCL8), which induced GC invasion and proliferation. This macrophage-induced GC progression was CXCL8 activated C-X-C Motif Chemokine Receptor 1/2 (CXCR1/2) on the GC cell membrane subsequently hyperactivated Janus kinase 1/ Signal transducer and activator of transcription 1 (JAK/STAT1) signaling pathway. Then, the transcription factor STAT1 directly led to the overexpression and secretion of Interleukin 10 (IL-10). Correspondingly, IL-10 induced the M2-type polarization of macrophages through the Nuclear Factor kappa B (NF-κB) pathway-dependent mechanism and continued to increase the expression and secretion of CXCL8 through the transcription factor Nuclear Factor Kappa B Subunit 1 (NFKB1, p50). It suggested a positive feedback loop between macrophage and GC. In clinical GC samples, increased CXCL8 predicted a patient's pessimistic outcome.Conclusion: Our work identified a positive feedback loop governing cancer cells and macrophage in GC that contributed to tumor progression and patient outcome.

scholarly journals The interplay between ATF2 and NEAT1 contributes to lung adenocarcinoma progression

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Jian Liu ◽  
Kai Li ◽  
Rui Wang ◽  
Sisi Chen ◽  
Jie Wu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Transcription Factor ◽  
Lung Adenocarcinoma ◽  
Positive Feedback ◽  
Feedback Loop ◽  
Tumor Model ◽  
Luciferase Reporter ◽  
Activator Protein 1 ◽  
Positive Feedback Loop ◽  
Rna Immunoprecipitation

Abstract Background Activating transcription factor 2 (ATF2), a member of the activator protein 1 (AP-1) transcription factor family, has been shown to be involved in the pathobiology of numerous cancers. However, the biological role and mechanism of ATF2 in lung adenocarcinoma (LUAD) remains to be elucidated. Methods The expression of ATF2, NEAT1 and miR-26a-5p in LUAD tissues and cell lines was detected by qRT-PCR and western blotting. The interaction between ATF2, NEAT1, and miR-26a-5p was validated by chromatin immunoprecipitation, luciferase reporter assay and RNA immunoprecipitation. Cell proliferation, invasion and tumorigenesis of LUAD cells were analyzed by using CCK8, transwell invasion assay and xenograft tumor model. Results We confirmed that ATF2 expression was increased in LUAD tissues compared with normal adjacent lung tissues. Functional experiments showed that ATF2 positively regulated cell proliferation and invasion in LUAD cells. Moreover, we identified that NEAT1 expression was increased in LUAD tissues and positively correlated with ATF2 expression. Mechanistically, ATF2 could bind to the promoter of NEAT1 to promote its transcription. Rescue experiments showed that ATF2 exerted its oncogenic function in LUAD, at least, partly through NEAT1 upregulation. In turn, NEAT1 could positively regulate ATF2 expression and form a positive feedback loop in LUAD cells. Furthermore, we demonstrated that NEAT1 positively regulated ATF2 expression via sponging miR-26a-5p. Conclusion ATF2 and NEAT1 form a positive feedback loop mediated by miR-26a-5p and coordinately contribute to LUAD progression.

scholarly journals Metabolic enzyme PDK3 forms a positive feedback loop with transcription factor HSF1 to drive chemoresistance

Theranostics ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 2999-3013 ◽  
Author(s):  
Jinye Xu ◽  
Qiqi Shi ◽  
Wenxia Xu ◽  
Qiyin Zhou ◽  
Rongkai Shi ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Positive Feedback ◽  
Feedback Loop ◽  
Metabolic Enzyme ◽  
Positive Feedback Loop

scholarly journals The oncogenic transcription factor IRF4 is regulated by a novel CD30/NF-κB positive feedback loop in peripheral T-cell lymphoma

Blood ◽  
2015 ◽  
Vol 125 (20) ◽  
pp. 3118-3127 ◽  
Author(s):  
Rebecca L. Boddicker ◽  
N. Sertac Kip ◽  
Xiaoming Xing ◽  
Yu Zeng ◽  
Zhi-Zhang Yang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Transcription Factor ◽  
Positive Feedback ◽  
Promoter Activity ◽  
Feedback Loop ◽  
Cell Lymphoma ◽  
Peripheral T Cell Lymphoma ◽  
Positive Feedback Loop ◽  
Key Points ◽  
Oncogenic Transcription Factor

Key Points The NF-κB subunits p52 and RelB increase IRF4 promoter activity and expression in PTCL cells. A positive feedback loop involving CD30, NF-κB, and IRF4 drives PTCL cell proliferation and can be blocked by NF-κB inhibitors.

scholarly journals A Rice NAC Transcription Factor Promotes Leaf Senescence via ABA Biosynthesis

2017 ◽  
Vol 174 (3) ◽  
pp. 1747-1763 ◽  
Author(s):  
Chanjuan Mao ◽  
Songchong Lu ◽  
Bo Lv ◽  
Bin Zhang ◽  
Jiabin Shen ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Leaf Senescence ◽  
Nac Transcription Factor ◽  
Aba Biosynthesis
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