Production of fibrolytic enzymes by Aspergillus japonicus C03 using agro-industrial residues with potential application as additives in animal feed

The agricultural activity has generated a progressive amount of waste, which needs a proper treatment to avoid negative environmental impacts. At the same time, values can be added to such waste, as its use in animal feed. This research was conducted at the laboratory of Animal Nutrition, State University of Southwestern Bahia, campuses of Vitória da Conquista and Itapetinga. The objective of this study was to evaluate the effect of coffee husks on ruminant feeds by increasing doses of fibrolytic enzymes, evaluating their effects on in vitro ruminal degradability of dry matter (DM), neutral detergent fiber (NDF), and acid detergent fiber (FDA) of the coffee husk (CH). The experiment was a completely randomized design in a 2x4 factorial scheme. It compounded the following treatments: Coffee husk (CH1): 1.5% enzymes (E) and 24 h enzymatic action (EA); CH2: 3.0% (E) and 24h (EA); CH3: 4.5% (E) and 24 h (EA); CH4: 6% (E) and 24 h (EA); CH5: 1.5% (E) 48 h (EA); CH6: 3% (E) and 48h (EA); CH7: 4.5% (E) and 48h (EA); and CH8: 6% (E) and 48 h (EA), all based on dry matter. An improvement in the degradability of the nutritional parameters MS, NDF, and FDA occurred with the addition of enzymes, with 3% enzyme addition being the best level, and 24 hours, as the best action time. In addition to that, as the EA on coffee husk increased, the degradation rate decreased. Therefore, the use of enzymes can improve the digestibility of the fibrous fraction, enabling the use of the coffee husk and possibly other agroindustrial residues, thus minimizing their adverse effects on nature.

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Comparative study of multi-enzyme production from typical agro-industrial residues and ultrasound-assisted extraction of crude enzyme in fermentation with Aspergillus japonicus PJ01

Bioprocess and Biosystems Engineering ◽

10.1007/s00449-015-1442-3 ◽

2015 ◽

Vol 38 (10) ◽

pp. 2013-2022 ◽

Cited By ~ 14

Author(s):

Pei-jun Li ◽

Jin-lan Xia ◽

Yang Shan ◽

Zhen-yuan Nie

Keyword(s):

Comparative Study ◽

Enzyme Production ◽

Crude Enzyme ◽

Ultrasound Assisted Extraction ◽

Aspergillus Japonicus ◽

Industrial Residues ◽

Assisted Extraction ◽

Ultrasound Assisted

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Hemicellulases from the thermophilic fungus Thermoascus aurantiacus - their potential application in pretreatment of animal feed

Proceedings of the British Society of Animal Science ◽

10.1017/s0308229600032013 ◽

1996 ◽

Vol 1996 ◽

pp. 238-238 ◽

Cited By ~ 1

Author(s):

S. Kalogiannis ◽

E. Owen ◽

D.E. Beever ◽

M.K. Bhat

Keyword(s):

Feed Intake ◽

Potential Application ◽

Animal Feed ◽

Thermophilic Fungus ◽

Thermoascus Aurantiacus ◽

Rotting Wood ◽

Partial Degradation ◽

Temperature Optima

Cellulose and hemicellulose are major components of forages for ruminants, and their partial degradation by chemical (e.g. NaOH, NH4OH) or biological (e.g. enzymes) means prior to feeding may improve both digestibility and feed intake. To identify suitable enzymes for forage treatment, it is necessary to establish their respective pH and temperature optima as well as their stability. In this study Thermoascus aurantiacus, a thermophilic fungus associated with rotting wood and self heated hay, was investigated as a source of stable hemicellulolytic enzymes.

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Characterization of the acerola industrial residues and prospection of their potential application as antioxidant dietary fiber source

Food Science and Technology ◽

10.1590/fst.18117 ◽

2018 ◽

Vol 38 (suppl 1) ◽

pp. 236-241 ◽

Cited By ~ 4

Author(s):

Jéssica Silva do CARMO ◽

Lailla Sabrina Queiroz NAZARENO ◽

Maria do Socorro Moura RUFINO

Keyword(s):

Dietary Fiber ◽

Potential Application ◽

Industrial Residues

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Adsorption of zearalenone by Aspergillus japonicus conidia: new trends for biological decontamination in animal feed

World Mycotoxin Journal ◽

10.3920/wmj2008.1128 ◽

2009 ◽

Vol 2 (4) ◽

pp. 391-397 ◽

Cited By ~ 4

Author(s):

G. Jard ◽

T. Liboz ◽

F. Mathieu ◽

A. Guyonvarc'h ◽

A. Lebrihi

Keyword(s):

Heat Treatment ◽

Animal Feed ◽

Animal Health ◽

Acidic Ph ◽

Fusarium Spp ◽

Aspergillus Japonicus ◽

Heat Treated ◽

Acidic Conditions ◽

Influence Of Ph

Zearalenone (ZEA) is an oestrogenic mycotoxin produced by Fusarium spp. on a wide variety of grains. The presence of ZEA impairs farm animal health. In this study, the ability to remove ZEA by conidia of Aspergilli was evaluated in vitro. This mycotoxin was successfully eliminated in vitro by living and heat-treated conidia just after conidial inoculation, especially by Aspergillus japonicus Mu541 (IMI389204) conidia. Consequently, components involved in adsorption are not affected by heat-treatment. The influence of pH on ZEA removal was also determined. Results have shown that ZEA removal was 53% at pH 2 while 2% removal was observed at pH 11. Then, the adsorption capacity was evaluated in vitro in porcine gastrointestinal tract conditions. We observed that there was better adsorption in acidic stomach conditions. Finally, the phenomenon has been modelled using Hill's equation. This model was very suitable and confirmed better adsorption at acidic pH. Aspergilli section Nigri conidia were able to adsorb ZEA instantaneously especially in acidic conditions as in the stomach. The use of heat-treated conidia could be a very interesting means of decreasing ZEA levels in animal feed. It must now be assessed directly on naturally contaminated feed and on animals.

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The Secretomes of Aspergillus japonicus and Aspergillus terreus Supplement the Rovabio® Enzyme Cocktail for the Degradation of Soybean Meal for Animal Feed

Journal of Fungi ◽

10.3390/jof7040278 ◽

2021 ◽

Vol 7 (4) ◽

pp. 278

Author(s):

Delphine Grandmontagne ◽

David Navarro ◽

Virginie Neugnot-Roux ◽

Simon Ladevèze ◽

Jean-Guy Berrin

Keyword(s):

Soybean Meal ◽

Aspergillus Terreus ◽

Plant Cell Wall ◽

Animal Feed ◽

Pectic Polysaccharides ◽

Aspergillus Japonicus ◽

Poultry Breeding ◽

Promising Solution ◽

Digestive Disorders ◽

Study Offer

One of the challenges of the 21st century will be to feed more than 10 billion people by 2050. In animal feed, one of the promising approaches is to use agriculture by-products such as soybean meal as it represents a rich source of proteins. However, soybean meal proteins are embedded in a complex plant cell wall matrix, mostly composed of pectic polysaccharides, which are recalcitrant to digestion for animals and can cause digestive disorders in poultry breeding. In this study, we explored fungal diversity to find enzymes acting on soybean meal components. An exploration of almost 50 fungal strains enabled the identification of two strains (Aspergillus terreus and Aspergillus japonicus), which improved the solubilization of soybean meal in terms of polysaccharides and proteins. The two Aspergilli strains identified in the frame of this study offer a promising solution to process industrial food coproducts into suitable animal feed solutions.

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