Isolation and expression analysis of four members of the plasma membrane H+-ATPase gene family in Hevea brasiliensis

V-ATPases are ubiquitous, vital proton pumps that play a multiplicity of roles in higher organisms. In many epithelia, they are the major energizer of cotransport processes and have been implicated in functions as diverse as fluid secretion and longevity. The first animal knockout of a V-ATPase was identified in Drosophila, and its recessive lethality demonstrated the essential nature of V-ATPases. This article surveys the entire V-ATPase gene family in Drosophila, both experimentally and in silico. Adult expression patterns of most of the genes are shown experimentally for the first time, using in situ hybridization or reporter gene expression, and these results are reconciled with published expression and microarray data. For each subunit, the single gene identified previously by microarray, as upregulated and abundant in tubules, is shown to be similarly abundant in other epithelia in which V-ATPases are known to be important; there thus appears to be a single dominant “plasma membrane” V-ATPase holoenzyme in Drosophila. This provides the most comprehensive view of V-ATPase expression yet in a multicellular organism. The transparent Malpighian tubule phenotype first identified in lethal alleles of vha55, the gene encoding the B-subunit, is shown to be general to those plasma membrane V-ATPase subunits for which lethal alleles are available, and to be caused by failure to accumulate uric acid crystals. These results coincide with the expression view of the gene family, in which 13 of the genes are specialized for epithelial roles, whereas others have spatially or temporally restricted patterns of expression.

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The plasma membrane H + -ATPase gene family in the arbuscular mycorrhizal fungus Glomus mosseae

Current Genetics ◽

10.1007/s002940050017 ◽

2000 ◽

Vol 37 (2) ◽

pp. 112-118 ◽

Cited By ~ 49

Author(s):

Nuria Ferrol ◽

José Miguel Barea ◽

Concepción Azcón-Aguilar

Keyword(s):

Plasma Membrane ◽

Gene Family ◽

Glomus Mosseae ◽

Arbuscular Mycorrhizal Fungus ◽

Mycorrhizal Fungus ◽

Arbuscular Mycorrhizal ◽

Atpase Gene

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An Expression Analysis of a Gene Family Encoding Plasma Membrane Aquaporins in Response to Abiotic Stresses in Arabidopsis Thaliana

Plant Molecular Biology ◽

10.1023/b:plan.0000040900.61345.a6 ◽

2004 ◽

Vol 54 (5) ◽

pp. 713-725 ◽

Cited By ~ 290

Author(s):

Ji Young Jang ◽

Dong Gu Kim ◽

Yeon Ok Kim ◽

Jin Sun Kim ◽

Hunseung Kang

Keyword(s):

Arabidopsis Thaliana ◽

Plasma Membrane ◽

Gene Family ◽

Expression Analysis ◽

Abiotic Stresses

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The plasma membrane H+-ATPase gene family in Solanum tuberosum L. Role of PHA1 in tuberization

Journal of Experimental Botany ◽

10.1093/jxb/erx284 ◽

2017 ◽

Vol 68 (17) ◽

pp. 4821-4837 ◽

Cited By ~ 4

Author(s):

Margarita Stritzler ◽

María Noelia Muñiz García ◽

Mariana Schlesinger ◽

Juan Ignacio Cortelezzi ◽

Daniela Andrea Capiati

Keyword(s):

Plasma Membrane ◽

Solanum Tuberosum ◽

Gene Family ◽

Solanum Tuberosum L ◽

Atpase Gene

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Genome-Wide Identification and Analysis of P-Type Plasma Membrane H+-ATPase Sub-Gene Family in Sunflower and the Role of HHA4 and HHA11 in the Development of Salt Stress Resistance

Genes ◽

10.3390/genes11040361 ◽

2020 ◽

Vol 11 (4) ◽

pp. 361 ◽

Cited By ~ 1

Author(s):

Zongchang Xu ◽

Prince Marowa ◽

Han Liu ◽

Haina Du ◽

Chengsheng Zhang ◽

...

Keyword(s):

Plasma Membrane ◽

Salt Stress ◽

Gene Family ◽

Abiotic Factors ◽

Pcr Analysis ◽

Future Research ◽

Genome Database ◽

Genome Wide ◽

Atpase Gene ◽

P Type

The P-type plasma membrane (PM) H+-ATPase plays a major role during the growth and development of a plant. It is also involved in plant resistance to a variety of biotic and abiotic factors, including salt stress. The PM H+-ATPase gene family has been well characterized in Arabidopsis and other crop plants such as rice, cucumber, and potato; however, the same cannot be said in sunflower (Helianthus annuus). In this study, a total of thirteen PM H+-ATPase genes were screened from the recently released sunflower genome database with a comprehensive genome-wide analysis. According to a systematic phylogenetic classification with a previously reported species, the sunflower PM H+-ATPase genes (HHAs) were divided into four sub-clusters (I, II, IV, and V). In addition, systematic bioinformatics analyses such as gene structure analysis, chromosome location analysis, subcellular localization predication, conserved motifs, and Cis-acting elements of promoter identification were also done. Semi-quantitative PCR analysis data of HHAs in different sunflower tissues revealed the specificity of gene spatiotemporal expression and sub-cluster grouping. Those belonging to sub-cluster I and II exhibited wide expression in almost all of the tissues studied while sub-cluster IV and V seldom showed expression. In addition, the expression of HHA4, HHA11, and HHA13 was shown to be induced by salt stress. The transgenic plants overexpressing HHA4 and HHA11 showed higher salinity tolerance compared with wild-type plants. Further analysis showed that the Na+ content of transgenic Arabidopsis plants decreased under salt stress, which indicates that PM H+ ATPase participates in the physiological process of Na+ efflux, resulting in salt resistance of the plants. This study is the first to identify and analyze the sunflower PM H+ ATPase gene family. It does not only lay foundation for future research but also demonstrates the role played by HHAs in salt stress tolerance.

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