Transcriptomic analysis reveals that coxsackievirus B3 Woodruff and GD strains use similar key genes to induce FoxO signaling pathway activation in HeLa cells

2021 ◽  
Author(s):  
Mi Liu ◽  
Qian Yang ◽  
Jun Han
Keyword(s):  
Signaling Pathway ◽  
Hela Cells ◽  
Coxsackievirus B3 ◽  
Transcriptomic Analysis ◽  
Pathway Activation ◽  
Key Genes ◽  
Foxo Signaling Pathway

scholarly journals Transcriptomic Analysis Reveals Both Coxsackievirus B3 Woodruff and GD Strain Using Similar Key Genes to Induce FoxO Signaling Pathway Activation in HeLa Cells

2021 ◽  
Author(s):  
Mi Liu ◽  
Qian Yang ◽  
Jun Han
Keyword(s):  
Oxidative Stress ◽  
Cell Cycle ◽  
Signaling Pathway ◽  
Hela Cells ◽  
Stress Resistance ◽  
Coxsackievirus B3 ◽  
Common Mechanism ◽  
Oxidative Stress Resistance ◽  
Key Genes ◽  
Foxo Signaling Pathway

Abstract Coxsackievirus B3 (CVB3) is the major cause of viral myocarditis in human worldwide. Various studies have investigated the viral infection and pathogenic mechanisms. However, the precise disease mechanism is still not clear. In this study, RNA-seq technology was used to compare the transcriptomic profile of virus infected HeLa cells to the controls in order to analysis the key genes of host virus interaction. Two CVB3 strains, CVB3 Woodruff and GD16-69-CVB3 strain were selected to figure out the common disease mechanisms of both experimental and clinical strains respectively. Increased expression of cell cycle genes CCNG2, GADD45B, PIM1, RBM15, KLF10 and RIOK3, down regulation of CYBA were detected. Autophagy genes ATG12 and YOD1 were also upregulated during CVB3 infection. Slightly increase of SOD2 and ATG12 were shown in the expression of infected cells, meanwhile, little change was detected in GABARAP expression. Further, FoxO signaling pathway was enriched by KEGG analysis, shown a close interaction with the DEGs in the PPI network. Genes of related pathways such as cell cycle, autophagy and oxidative stress resistance were confirmed by RT-PCR as well. In conclusion, our results reveal that FoxO signaling pathway is a common mechanism activated during the infection of both CVB3 strains. And this pathway plays a regulatory role in downstream pathways such as cell cycle, autophagy, oxidative stress resistance and antiviral immune responds.

scholarly journals The Transplantation Resistance of Type II Diabetes Mellitus Adipose-Derived Stem Cells Is Due to G6PC and IGF1 Genes Related to the FoxO Signaling Pathway

2021 ◽  
Vol 22 (12) ◽  
pp. 6595
Author(s):  
Michiko Horiguchi ◽  
Yuya Turudome ◽  
Kentaro Ushijima
Keyword(s):  
Diabetes Mellitus ◽  
Stem Cells ◽  
Stem Cell ◽  
Stem Cell Transplantation ◽  
Signaling Pathway ◽  
Cell Transplantation ◽  
Type Ii ◽  
Lower Performance ◽  
Foxo Signaling Pathway ◽  
Healthy Persons

In cases of patients with rapidly progressive diabetes mellitus (DM), autologous stem cell transplantation is considered as one of the regenerative treatments. However, whether the effects of autonomous stem cell transplantation on DM patients are equivalent to transplantation of stem cells derived from healthy persons is unclear. This study revealed that adipose-derived mesenchymal stem cells (ADSC) derived from type II DM patients had lower transplantation efficiency, proliferation potency, and stemness than those derived from healthy persons, leading to a tendency to induce apoptotic cell death. To address this issue, we conducted a cyclopedic mRNA analysis using a next-generation sequencer and identified G6PC3 and IGF1, genes related to the FoxO signaling pathway, as the genes responsible for lower performance. Moreover, it was demonstrated that the lower transplantation efficiency of ADSCs derived from type II DM patients might be improved by knocking down both G6PC3 and IGF1 genes. This study clarified the difference in transplantation efficiency between ADSCs derived from type II DM patients and those derived from healthy persons and the genes responsible for the lower performance of the former. These results can provide a new strategy for stabilizing the quality of stem cells and improving the therapeutic effects of regenerative treatments on autonomous stem cell transplantation in patients with DM.

scholarly journals Studies of the Anti-Diabetic Mechanism of Pueraria lobata Based on Metabolomics and Network Pharmacology

Processes ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 1245
Author(s):  
Shu Zhang ◽  
Qi Ge ◽  
Liang Chen ◽  
Keping Chen
Keyword(s):  
Signaling Pathway ◽  
Diabetes Complications ◽  
Mapk Signaling ◽  
Network Pharmacology ◽  
Pueraria Lobata ◽  
Insulin Deficiency ◽  
Active Ingredients ◽  
Foxo Signaling Pathway ◽  
Anti Inflammation

Diabetes mellitus (DM), as a chronic disease caused by insulin deficiency or using obstacles, is gradually becoming a principal worldwide health problem. Pueraria lobata is one of the traditional Chinese medicinal and edible plants, playing roles in improving the cardiovascular system, lowering blood sugar, anti-inflammation, anti-oxidation, and so on. Studies on the hypoglycemic effects of Pueraria lobata were also frequently reported. To determine the active ingredients and related targets of Pueraria lobata for DM, 256 metabolites were identified by LC/MS non targeted metabonomics, and 19 active ingredients interacting with 51 DM-related targets were screened. The results showed that puerarin, quercetin, genistein, daidzein, and other active ingredients in Pueraria lobata could participate in the AGE-RAGE signaling pathway, insulin resistance, HIF-1 signaling pathway, FoxO signaling pathway, and MAPK signaling pathway by acting on VEGFA, INS, INSR, IL-6, TNF and AKT1, and may regulate type 2 diabetes, inflammation, atherosis and diabetes complications, such as diabetic retinopathy, diabetic nephropathy, and diabetic cardiomyopathy.

scholarly journals Regulation of Chemokine Production via Oxidative Pathway in HeLa Cells

2009 ◽  
Vol 2009 ◽  
pp. 1-5 ◽  
Author(s):  
Shinichiro Kina ◽  
Toshiyuki Nakasone ◽  
Hiroyuki Takemoto ◽  
Akira Matayoshi ◽  
Shoko Makishi ◽  
...  
Keyword(s):  
Hela Cells ◽  
Signal Transduction Pathway ◽  
Iron Chelator ◽  
Enzyme Linked Immunosorbent Assay ◽  
Oxygen Species ◽  
Chemokine Production ◽  
Pathway Activation ◽  
Oxidative Pathway ◽  
Erk2 Activation ◽  
Culture Supernatants

Inflammation is associated with disease progression and, by largely unknown mechanisms, has been said to drive oncogenesis. At inflamed sites, neutrophils deploy a potent antimicrobial arsenal that includes proteinases, antimicrobial peptides, and ROS. Reactive oxygen species (ROSs) induce chemokines. In the present study, the concentrations of IL-8 in culture supernatants of HeLa cells treated with ROS were determined by enzyme-linked immunosorbent assay. We used -phenanthroline to deplete in order to investigate the mechanisms through which ROSs induce IL-8 secretion in our system. The iron chelator -phenanthroline effectively inhibited -induced ERK2 activation. Enzyme-linked immunosorbent assays showed that IL-8 protein secretion was elevated in ROS-treated HeLa cells. When was removed from these cells, IL-8 secretion was inhibited. Collectively, these results indicate that -mediated Erk pathway activation is an important signal transduction pathway in ROS-induced IL-8 secretion in epithelial cells.

The Early Interaction of Coxsackievirus B3 with HeLa Cells

1971 ◽  
Vol 137 (3) ◽  
pp. 1082-1088 ◽  
Author(s):  
R. L. Crowell ◽  
B. J. Landau ◽  
L. Philipson
Keyword(s):  
Hela Cells ◽  
Coxsackievirus B3 ◽  
Early Interaction
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