Elevated CO2 alters tissue balance of nitrogen metabolism and downregulates nitrogen assimilation and signalling gene expression in wheat seedlings receiving high nitrate supply

To explore the response of nitrogen metabolism in Masson pine (Pinus massoniana) to high CO2 concentrations, needles from one-year-old seedlings were used as materials to detect key enzyme activities, gene expression and different forms of nitrogen metabolites after CO2 stress for different durations (0 h, 6 h, 12 h, 24 h). The results show that elevated CO2 affected the efficiency of nitrogen metabolism in Masson pine needles, inhibiting the expression of key genes involved in nitrogen metabolism, including glutamate synthase (GOGAT), nitrite reductase (NiR), glutamine synthase (GS), nitrate reductase (NR) and glutamate dehydrogenase (GDH), and decreasing the activities of GOGAT, NiR, and GS. The decrease in enzyme activities and gene expression caused a decrease in different forms of nitrogen metabolites, including total nitrogen, ammonium, nitrite and specific amino acids. With prolonged stress, the nitrate content increased first and then decreased. In this study, the response pattern of nitrogen metabolism to CO2 stress in Masson pine needles was described, which may aid future research on nitrogen utilization in Masson pine.

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Nitrogen assimilation and gene regulation of two Kentucky bluegrass cultivars differing in response to nitrate supply

Scientia Horticulturae ◽

10.1016/j.scienta.2021.110315 ◽

2021 ◽

Vol 288 ◽

pp. 110315

Author(s):

Xiaoyang Sun ◽

Qianjiao Zheng ◽

Liangbing Xiong ◽

Fuchun Xie ◽

Xun Li ◽

...

Keyword(s):

Gene Regulation ◽

Nitrogen Assimilation ◽

Kentucky Bluegrass ◽

Nitrate Supply

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Plant nitrate supply regulates Erwinia amylovora virulence gene expression in Arabidopsis

Molecular Plant Pathology ◽

10.1111/mpp.13114 ◽

2021 ◽

Author(s):

Mahsa Farjad ◽

Gilles Clément ◽

Alban Launay ◽

Roua Jeridi ◽

Sylvie Jolivet ◽

...

Keyword(s):

Gene Expression ◽

Erwinia Amylovora ◽

Virulence Gene ◽

Virulence Gene Expression ◽

Nitrate Supply

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Effects of root restriction on nitrogen and gene expression levels in nitrogen metabolism in Jumeigui grapevines (Vitis vinifera L.×Vitis labrusca L.)

Journal of Integrative Agriculture ◽

10.1016/s2095-3119(14)60876-5 ◽

2015 ◽

Vol 14 (1) ◽

pp. 67-79 ◽

Cited By ~ 4

Author(s):

Xiu-ming YU ◽

Jie-fa LI ◽

Li-na ZHU ◽

Bo WANG ◽

Lei WANG ◽

...

Keyword(s):

Gene Expression ◽

Vitis Vinifera ◽

Nitrogen Metabolism ◽

Vitis Vinifera L ◽

Vitis Labrusca ◽

Expression Levels ◽

Root Restriction ◽

Gene Expression Levels

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The impact of elevated atmospheric CO2 and nitrate supply on growth, biomass allocation, nitrogen partitioning and N2 fixation of Acacia melanoxylon

Functional Plant Biology ◽

10.1071/pp99062 ◽

1999 ◽

Vol 26 (8) ◽

pp. 737 ◽

Cited By ~ 20

Author(s):

Marcus Schortemeyer ◽

Owen K. Atkin ◽

Nola McFarlane ◽

John R. Evans

Keyword(s):

Elevated Co2 ◽

N2 Fixation ◽

Dry Matter ◽

Co2 Concentration ◽

Dry Mass ◽

Nitrogen Partitioning ◽

Atmospheric Co2 ◽

Acacia Melanoxylon ◽

Nitrate Supply ◽

The Impact

The interactive effects of nitrate supply and atmospheric CO2 concentration on growth, N2 fixation, dry matter and nitrogen partitioning in the leguminous tree Acacia melanoxylon R.Br. were studied. Seedlings were grown hydroponically in controlled-environment cabinets for 5 weeks at seven 15N-labelled nitrate levels, ranging from 3 to 6400 mmol m–3. Plants were exposed to ambient (~350 µmol mol–1) or elevated (~700 µmol mol–1) atmospheric CO2 for 6 weeks. Total plant dry mass increased strongly with nitrate supply. The proportion of nitrogen derived from air decreased with increasing nitrate supply. Plants grown under either ambient or elevated CO2 fixed the same amount of nitrogen per unit nodule dry mass (16.6 mmol N per g nodule dry mass) regardless of the nitrogen treatment. CO2 concentration had no effect on the relative contribution of N2 fixation to the nitrogen yield of plants. Plants grown with ≥50 mmol m–3 N and elevated CO2 had approximately twice the dry mass of those grown with ambient CO2 after 42 days. The rates of net CO2 assimilation under growth conditions were higher per unit leaf area for plants grown under elevated CO2. Elevated CO2 also decreased specific foliage area, due to an increase in foliage thickness and density. Dry matter partitioning between plant organs was affected by ontogeny and nitrogen status of the plants, but not by CO2 concentration. In contrast, plants grown under elevated CO2 partitioned more of their nitrogen to roots. This could be attributed to reduced nitrogen concentrations in foliage grown under elevated CO2.

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Nutrient Remobilization, Nitrogen Metabolism and Chloroplast Gene Expression in Senescent Leaves

Plant Aging ◽

10.1007/978-1-4684-5760-5_27 ◽

1990 ◽

pp. 225-229

Author(s):

Bartolomé Sabater ◽

Antonio Vera ◽

Rafael Tomás ◽

Mercedes Martin

Keyword(s):

Gene Expression ◽

Nitrogen Metabolism ◽

Chloroplast Gene ◽

Chloroplast Gene Expression ◽

Nutrient Remobilization

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Differential Growth Responses of Wheat Seedlings to Elevated CO2

Notulae Scientia Biologicae ◽

10.15835/nsb10310286 ◽

2018 ◽

Vol 10 (3) ◽

pp. 400-409 ◽

Cited By ~ 3

Author(s):

Hamid Reza ESHGHIZADEH ◽

Morteza ZAHEDI ◽

Samaneh MOHAMMADI

Keyword(s):

Leaf Area ◽

Plant Height ◽

Elevated Co2 ◽

Co2 Concentration ◽

Tolerance Index ◽

Dry Matter Accumulation ◽

Wheat Cultivars ◽

Growth Responses ◽

Differential Growth ◽

Wheat Seedlings

Intraspecific variations in wheat growth responses to elevated CO2 was evaluated using 20 Iranian bread wheat (Triticum aestivum L.) cultivars. The plants were grown in the modified Hoagland nutrient solution at a greenhouse until 35 days of age using two levels of CO2 (~380 and 700 µmol mol–1). The shoot and root dry weights of the wheat cultivars exhibited average enhancements of 17% and 36%, respectively, under elevated CO2. This increase was associated with higher levels of chlorophyll a (25%), chlorophyll b (21%), carotenoid (30%), leaf area (54%) and plant height (49.9%). The leaf area (r = 0.69**), shoot N content (r = 0.62**), plant height (r = 0.60**) and root volume (r = 0.53*) were found to have important roles in dry matter accumulation of tested wheat cultivars under elevated CO2 concentration. However, responses to elevated CO2 were considerably cultivar-dependent. Based on the stress susceptibility index (SSI) and stress tolerance index (STI), the wheat cultivars exhibiting the best response to elevated CO2 content were ‘Sistan’, ‘Navid’, ‘Shiraz’, ‘Sepahan’ and ‘Bahar’, while the ones with poor responses were ‘Omid’, ‘Marun’, ‘Sorkhtokhm’ and ‘Tajan’. The findings from the present experiment showed significant variation among the Iranian wheat cultivars in terms of their responses to elevated air CO2, providing the opportunity to select the most efficient ones for breeding purposes.

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