Proto-dolomite formation in microbial consortia dominated by Halomonas strains

Extremophiles ◽  
2019 ◽  
Vol 23 (6) ◽  
pp. 765-781
Author(s):  
Ammar Alibrahim ◽  
Dunia Al-Gharabally ◽  
Huda Mahmoud ◽  
Maria Dittrich
Keyword(s):  
Microbial Consortia ◽  
Dolomite Formation

scholarly journals Dolomite genesis in bioturbated marine zones of an early-middle Miocene coastal mud volcano outcrop (Kuwait)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ammar Alibrahim ◽  
Michael J. Duane ◽  
Maria Dittrich
Keyword(s):  
Middle Miocene ◽  
Saturation Index ◽  
Mud Volcano ◽  
Microbial Consortia ◽  
Charged Fluids ◽  
Ce Anomaly ◽  
Eu Anomaly ◽  
Dolomite Formation ◽  
Reducing Bacteria ◽  
Dolomite Precipitation

AbstractThe origin of spheroidal dolomitized burrow from Al-Subiya sabkha in Kuwait was previously described as enigmatic as no evidence of precursor calcium carbonate was found in the siliciclastic sediment. An assumption for the genesis of spheroidal dolomite from the same area was attributed to hydrocarbon seepage but no evidence was provided. Here, we investigated a recently discovered early-middle Miocene coastal mud volcano outcrop in Al-Subiya sabkha where dolomitized burrows and spheroidal dolomite are found in bioturbated marine zones, and associated with traces of salt. Conversely, the continental zone lacks bioturbation features, dolomite and traces of salt, which together contrast with bioturbated rich marine zones. Geochemical signatures of Rare Earth Elements + Yttrium show a true positive Ce anomaly (Ce/Ce* > 1.2) and positive Eu/Eu* anomaly of spheroidal dolomite indicating strictly anoxic conditions, and sulphate reduction to sulphide, respectively. Our results are suggestive of a relationship between dolomite formation and interdependent events of hydrocarbon seepage, flux of hypersaline seawater, bioturbation, and fluid flow in the marine zones of the mud volcano. The bioturbation activity of crustaceans introduced channels/burrows in the sediment–water interface allowing for the mixing of seeped pressurized hydrocarbon-charged fluids, and evaporitic seawater. In the irrigated channels/burrows, the seeped pressurized hydrocarbon-charged fluids were oxidized via microbial consortia of methanotrophic archaea and sulphate-reducing bacteria resulting in elevated alkalinity and saturation index with respect to dolomite, thus providing the preferential geochemical microenvironment for dolomite precipitation in the bioturbated sediment.

Unravelling a microbial synergy to boost caproate production via carboxylates chain elongation with ethanol

2019 ◽  
Vol 26 (2) ◽  
pp. 63-71
Author(s):  
Ling Leng ◽  
Ying Wang ◽  
Peixian Yang ◽  
Takashi Narihiro ◽  
Masaru Konishi Nobu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Microbial Consortia ◽  
Chain Elongation ◽  
Desulfovibrio Vulgaris ◽  
Rrna Gene ◽  
Selective Enrichment ◽  
Microbial Network ◽  
Cost Efficient ◽  
Rrna Gene Sequencing

Chain elongation of volatile fatty acids for medium chain fatty acids production (e.g. caproate) is an attractive approach to treat wastewater anaerobically and recover resource simultaneously. Undefined microbial consortia can be tailored to achieve chain elongation process with selective enrichment from anaerobic digestion sludge, which has advantages over pure culture approach for cost-efficient application. Whilst the metabolic pathway of the dominant caproate producer, Clostridium kluyveri, has been annotated, the role of other coexisting abundant microbiomes remained unclear. To this end, an ethanol-acetate fermentation inoculated with fresh digestion sludge at optimal conditions was conducted. Also, physiological study, thermodynamics and 16 S rRNA gene sequencing to elucidate the biological process by linking the system performance and dominant microbiomes were integrated. Results revealed a possible synergistic network in which C. kluyveri and three co-dominant species, Desulfovibrio vulgaris, Fusobacterium varium and Acetoanaerobium sticklandii coexisted. D. vulgaris and A. sticklandii (F. varium) were likely to boost the carboxylates chain elongation by stimulating ethanol oxidation and butyrate production through a syntrophic partnership with hydrogen (H2) serving as an electron messenger. This study unveils a synergistic microbial network to boost caproate production in mixed culture carboxylates chain elongation.

Learning From Nature: Using Microbial Consortia To Improve Biochemical Synthesis

2018 ◽  
Author(s):  
Nicholas A. Kaplan ◽  
Alexandra M. Adams ◽  
J. Andrew Jones
Keyword(s):  
Microbial Consortia

An efficient biotreatment process for polyvinyl alcohol containing textile wastewater

2013 ◽  
Vol 8 (3-4) ◽  
pp. 469-478 ◽  
Author(s):  
Sandip S. Magdum ◽  
Gauri P. Minde ◽  
Upendra S. Adhyapak ◽  
V. Kalyanraman
Keyword(s):  
Polyvinyl Alcohol ◽  
Process Optimization ◽  
Textile Wastewater ◽  
Cost Effective ◽  
Chemical Oxidation ◽  
High Rate ◽  
Microbial Consortia ◽  
Environment Friendly ◽  
Candida Sp ◽  
Textile Wastewater Treatment

The aim of this work was to optimize the biodegradation of polyvinyl alcohol (PVA) containing actual textile wastewater for a sustainable treatment solution. The isolated microbial consortia of effective PVA degrader namely Candida Sp. and Pseudomonas Sp., which were responsible for symbiotic degradation of chemical oxidation demand (COD) and PVA from desizing wastewater. In the process optimization, the maximum aeration was essential to achieve a high degradation rate, where as stirring enhances further degradation and foam control. Batch experiments concluded with the need of 16 lpm/l and 150 rpm of air and stirring speed respectively for high rate of COD and PVA degradation. Optimized process leads to 2 days of hydraulic retention time (HRT) with 85–90% PVA degradation. Continuous study also confirmed above treatment process optimization with 85.02% of COD and 90.3% of PVA degradation of effluent with 2 days HRT. This study gives environment friendly and cost effective solution for PVA containing textile wastewater treatment.

scholarly journals Acinetobacter tandoii ZM06 Assists Glutamicibacter nicotianae ZM05 in Resisting Cadmium Pressure to Preserve Dipropyl Phthalate Biodegradation

2021 ◽  
Vol 9 (7) ◽  
pp. 1417
Author(s):  
Xuejun Wang ◽  
Si Shen ◽  
Hao Wu ◽  
Haixia Wang ◽  
Lvjing Wang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Heavy Metals ◽  
Microbial Consortia ◽  
Environmental Media ◽  
Degrading Bacterium ◽  
Coculture System ◽  
Heavy Metals Contamination ◽  
Metabolite Inhibition ◽  
Comparative Transcriptomic Analysis ◽  
Scanning Electron

Dipropyl phthalate (DPrP) coexists with cadmium as cocontaminants in environmental media. A coculture system including the DPrP-degrading bacterium Glutamicibacter nicotianae ZM05 and the nondegrading bacterium Acinetobacter tandoii ZM06 was artificially established to degrade DPrP under Cd(II) stress. Strain ZM06 relieved the pressure of cadmium on strain ZM05 and accelerated DPrP degradation in the following three ways: first, strain ZM06 adsorbed Cd(II) on the cell surface (as observed by scanning electron microscopy) to decrease the concentration of Cd(II) in the coculture system; second, the downstream metabolites of ZM05 were utilized by strain ZM06 to reduce metabolite inhibition; and third, strain ZM06 supplied amino acids and fatty acids to strain ZM05 to relieve stress during DPrP degradation, which was demonstrated by comparative transcriptomic analysis. This study provides an elementary understanding of how microbial consortia improve the degradation efficiency of organic pollutants under heavy metals contamination.

scholarly journals Metagenome-assembled genomes infer potential microbial metabolism in alkaline sulphidic tailings

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Wenjun Li ◽  
Xiaofang Li
Keyword(s):  
Community Structure ◽  
Mine Tailings ◽  
Fluorescent In Situ Hybridization ◽  
High Throughput Sequencing ◽  
Microbial Consortia ◽  
Metabolic Reconstruction ◽  
Metal Release ◽  
Community Members ◽  
Oxidative Stresses

Abstract Background Mine tailings are hostile environment. It has been well documented that several microbes can inhabit such environment, and metagenomic reconstruction has successfully pinpointed their activities and community structure in acidic tailings environments. We still know little about the microbial metabolic capacities of alkaline sulphidic environment where microbial processes are critically important for the revegetation. Microbial communities therein may not only provide soil functions, but also ameliorate the environment stresses for plants’ survival. Results In this study, we detected a considerable amount of viable bacterial and archaeal cells using fluorescent in situ hybridization in alkaline sulphidic tailings from Mt Isa, Queensland. By taking advantage of high-throughput sequencing and up-to-date metagenomic binning technology, we reconstructed the microbial community structure and potential coupled iron and nitrogen metabolism pathways in the tailings. Assembly of 10 metagenome-assembled genomes (MAGs), with 5 nearly complete, was achieved. From this, detailed insights into the community metabolic capabilities was derived. Dominant microbial species were seen to possess powerful resistance systems for osmotic, metal and oxidative stresses. Additionally, these community members had metabolic capabilities for sulphide oxidation, for causing increased salinity and metal release, and for leading to N depletion. Conclusions Here our results show that a considerable amount of microbial cells inhabit the mine tailings, who possess a variety of genes for stress response. Metabolic reconstruction infers that the microbial consortia may actively accelerate the sulphide weathering and N depletion therein.

scholarly journals Synthetic neural-like computing in microbial consortia for pattern recognition

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Ximing Li ◽  
Luna Rizik ◽  
Valeriia Kravchik ◽  
Maria Khoury ◽  
Netanel Korin ◽  
...  
Keyword(s):  
Neural Networks ◽  
Artificial Neural Networks ◽  
Gradient Descent ◽  
Biological Systems ◽  
Logic Gates ◽  
Structural Similarity ◽  
Microbial Consortia ◽  
Promoter Strength ◽  
Chemical Inducers ◽  
Artificial Neural

AbstractComplex biological systems in nature comprise cells that act collectively to solve sophisticated tasks. Synthetic biological systems, in contrast, are designed for specific tasks, following computational principles including logic gates and analog design. Yet such approaches cannot be easily adapted for multiple tasks in biological contexts. Alternatively, artificial neural networks, comprised of flexible interactions for computation, support adaptive designs and are adopted for diverse applications. Here, motivated by the structural similarity between artificial neural networks and cellular networks, we implement neural-like computing in bacteria consortia for recognizing patterns. Specifically, receiver bacteria collectively interact with sender bacteria for decision-making through quorum sensing. Input patterns formed by chemical inducers activate senders to produce signaling molecules at varying levels. These levels, which act as weights, are programmed by tuning the sender promoter strength Furthermore, a gradient descent based algorithm that enables weights optimization was developed. Weights were experimentally examined for recognizing 3 × 3-bit pattern.

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