Formulation of the biocontrol agent Bacillus amyloliquefaciens CPA-8 using different approaches: liquid, freeze-drying and fluid-bed spray-drying

Background: S. chinensis extract contains bioactive compounds, which exhibit high antioxidant activities. However, for commercial uses, it is necessary to encapsulate the extract to protect it from degradation. Objective: This study aimed to optimise spray-drying conditions and then compare with freeze-drying to identify the most suitable conditions for encapsulation of Salacia chinensis L. root extract. Method: Three factors of spray-drying encapsulation, including maltodextrin concentration, inlet temperature and feed rate, have been tested for the impacts on the physical and phytochemical properties of S. chinensis root extract. Based on the optimal conditions, the spray-drying was then compared with freeze-drying. Results: The results showed that maltodextrin concentration, inlet temperature and feed rate had significant impacts on recovery yield, phenolics, mangiferin and antioxidant activity of the spray-dried extract. The optimal spray-drying encapsulation conditions were maltodextrin concentration of 20 %, inlet temperature of 130ºC and feed rate of 9 mL/min. Under these optimal conditions, the encapsulated extract had comparable solubility, total phenolics, mangiferin, and antioxidant activity, lower bulk density, moisture content, and water activity as compared to encapsulated extract made using the freeze-drying technique. These optimal spray-drying conditions are recommended to encapsulate the extract of S. chinensis root. Conclusion: Spray-drying was found to be more effective for encapsulation of S. chinensis root extract than freeze-drying. Therefore, spray-drying is recommended for further applications.

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Potential of an endophytic bacteria Bacillus amyloliquefaciens 3–5 as biocontrol agent against potato scab

Microbial Pathogenesis ◽

10.1016/j.micpath.2021.105382 ◽

2021 ◽

pp. 105382

Author(s):

Lingxiao Cui ◽

Chengde Yang ◽

Yinyu Wang ◽

Ting Ma ◽

Fengfeng Cai ◽

...

Keyword(s):

Bacillus Amyloliquefaciens ◽

Endophytic Bacteria ◽

Biocontrol Agent ◽

Potato Scab

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Comparison between freeze and spray drying to obtain powder Rubrivivax gelatinosus biomass

Food Science and Technology ◽

10.1590/s0101-20612013005000008 ◽

2013 ◽

Vol 33 (1) ◽

pp. 47-51 ◽

Cited By ~ 12

Author(s):

Edson Francisco do Espírito Santo ◽

Leandro Kanamaru Franco de Lima ◽

Ane Pamela Capucci Torres ◽

Gabriela de Oliveira ◽

Elisa Helena Giglio Ponsano

Keyword(s):

Spray Drying ◽

Freeze Drying ◽

Bacterial Biomass ◽

Quality Criterion ◽

Industrial Effluents ◽

Product Recovery ◽

Animal Origin ◽

Freeze Dried ◽

Rubrivivax Gelatinosus ◽

Industry Effluent

The use of colorants in products of animal origin is justified by the improvement in the color of foods since this attribute is considered a quality criterion. These additives can be produced using industrial effluents as substrates and appropriate organisms, such as Rubrivivax gelatinosus. Oxycarotenoids represent a class of carotenes responsible for the pigmentation of animals and vegetables. R. gelatinosus grows in fish industry effluent with the resulting production of a bacterial biomass containing oxycarotenoids. The purpose of this study was to compare the use of two drying processes - spray and freeze drying - to obtain powder biomass in terms of the process parameters (yield, productivity, and product recovery) and the product characteristics (color, proximate composition, and oxycarotenoids). No difference was detected in the yield between these techniques, while productivity was higher using spray drying. Higher product recovery and moisture were achieved with freeze drying, while ash was higher with spray drying. The freeze dried biomass was redder, darker and less saturated than the spray dried biomass. No difference in oxycarotenoids was detected between the biomasses. Although it results in lower recovery rate, spray drying was faster and more productive, and it provided the same yield as freeze drying, which makes it the method of choice for obtaining R. gelatinosus biomass.

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