Epstein–Barr virus is seldom found in mammary epithelium of breast cancer tissue using in situ molecular methods

2011 ◽  
Vol 132 (1) ◽  
pp. 267-274 ◽  
Author(s):  
Kimberly Baltzell ◽  
Gertrude C. Buehring ◽  
Savitri Krishnamurthy ◽  
Henry Kuerer ◽  
Hua Min Shen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Epstein Barr Virus ◽  
Mammary Epithelium ◽  
Breast Cancer Tissue ◽  
Molecular Methods ◽  
Cancer Tissue ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Epstein-Barr Virus (EBV) Genome and Expression in Breast Cancer Tissue: Effect of EBV Infection of Breast Cancer Cells on Resistance to Paclitaxel (Taxol)

2006 ◽  
Vol 80 (2) ◽  
pp. 845-853 ◽  
Author(s):  
Hratch Arbach ◽  
Viktor Viglasky ◽  
Florence Lefeu ◽  
Jean-Marc Guinebretière ◽  
Vanessa Ramirez ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Breast Cancer Cells ◽  
Epstein Barr Virus ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr

ABSTRACT The Epstein-Barr virus (EBV) has been detected in subsets of breast cancers. In order to elaborate on these observations, we quantified by real-time PCR (Q-PCR) the EBV genome in biopsy specimens of breast cancer tissue as well as in tumor cells isolated by microdissection. Our findings show that EBV genomes can be detected by Q-PCR in about half of tumor specimens, usually in low copy numbers. However, we also found that the viral load is highly variable from tumor to tumor. Moreover, EBV genomes are heterogeneously distributed in morphologically identical tumor cells, with some clusters of isolated tumor cells containing relatively high genome numbers while other tumor cells isolated from the same specimen may be negative for EBV DNA. Using reverse transcription-PCR, we detected EBV gene transcripts: EBNA-1 in almost all of the EBV-positive tumors and RNA of the EBV oncoprotein LMP-1 in a smaller subset of the tissues analyzed. Moreover, BARF-1 RNA was detected in half of the cases studied. Furthermore, we observed that in vitro EBV infection of breast carcinoma cells confers resistance to paclitaxel (taxol) and provokes overexpression of a multidrug resistance gene (MDR1). Consequently, even if a small number of breast cancer cells are EBV infected, the impact of EBV infection on the efficiency of anticancer treatment might be of importance.

scholarly journals Molecular Epidemiology of Epstein-Barr Virus in Women Breast Cancer in Congo Brazzaville

2021 ◽  
Vol 11 (11) ◽  
pp. 180-190
Author(s):  
Dimitry Moudiongui Mboungou Malanda ◽  
Anicet Luc Magloire Boumba ◽  
Fabien Gaël Mouamba
Keyword(s):  
Breast Cancer ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Barr Virus ◽  
Significant Difference ◽  
Ebv Dna ◽  
Epstein Barr ◽  
The Republic ◽  
Congo Brazzaville ◽  
Formalin Fixed

Introduction: the Epstein Barr virus is one of the very first oncogenic viruses to be identified as responsible for human malignancies. Its role as an etiological agent of breast cancer remains controversial, however, despite the growing molecular evidence. The aim of this study was detected the presence of EBV DNA in patients with breast cancer in the Republic of Congo. Methods: The study was conducted on 90 samples of formalin fixed and paraffin-embedded tissue blocks (FFPE) from breast cancer tissue. The immunohistochemistry technique was used to test for the expression of the LMP1 antibody and DNA was extracted from all blocks of formalin-fixed and paraffin-embedded breast cancer tissue (FFPE) to detect presence of EBV 1 DNA by real-time polymerase chain reaction (PCR). Results: EBV was detected in 12.33% (12/90) of formalin-fixed, paraffin-embedded (FFPE) breast cancer tissue blocks. All formalin-fixed, paraffin-embedded (FFPE) breast cancer tissue blocks with positive EBV DNA were high tumor grades (II and III). Overall EBV infection with clinicopathological features of breast cancer cases showed no significant difference (P>0.05). However, a statistically significant difference was observed between EBV infection and histological types (P=0.04). Conclusion: Our results provide evidence for the presence of EBV DNA in female breast cancer in Congo Brazzaville. However, this evidence is substantial but inconclusive for the involvement of viruses in the development of breast cancer. Therefore, future investigations will be needed to elucidate the exact role of EBV in breast cancer in women in the Republic of Congo. Key words: EBV, breast cancer, women, Congo Brazzaville.

Evidence for the association of Epstein-Barr Virus in breast cancer in Indian patients using in-situ hybridization technique

2017 ◽  
Vol 24 (1) ◽  
pp. 16-22 ◽  
Author(s):  
Trupti Pai ◽  
Sudeep Gupta ◽  
Mamta Gurav ◽  
Shona Nag ◽  
Tanuja Shet ◽  
...  
Keyword(s):  
Breast Cancer ◽  
In Situ Hybridization ◽  
Epstein Barr Virus ◽  
Hybridization Technique ◽  
Barr Virus ◽  
Indian Patients ◽  
Epstein Barr

scholarly journals Molecular detection of Epstein-Barr virus in breast cancer among Sudanese female population: a case-control study

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 1355
Author(s):  
Eiman S. Ahmed ◽  
Lubna S. Elnour ◽  
Emmanuel E. Siddig ◽  
Rowa Hassan
Keyword(s):  
Breast Cancer ◽  
Epstein Barr Virus ◽  
Case Control Study ◽  
Case Control ◽  
Control Group ◽  
Cancer Tissue ◽  
Female Population ◽  
Barr Virus ◽  
Epstein Barr ◽  
Control Study

Background: Breast cancer is the most common cancer in women worldwide and in Sudan. Breast cancer occurs due to a multifactorial process and infection with an oncogenic virus has been recently investigated as a possible risk factor for breast cancer. For nearly two decades, studies have incriminated Epstein-Barr virus (EBV) in the etiology of breast cancer. However, the results are unconvincing, and their interpretation has remained a matter of debate. The aim of this study was to detect EBV in breast cancer biopsies obtained from Sudanese female patients. Methods: A descriptive, hospital-based, case-control study, conducted at Faculty of Medical Laboratory Science, University of Khartoum, Khartoum, Sudan. Archival blocks were obtained from 115 patients with breast cancer and 115 controls during the period between November 2016 till March 2017. Results: Among 115 breast cancer tissue specimens, EBV DNA was identified in 42/115 (36.5 %) samples and was not identified in 73/115 (63.5 %) tissue samples. The highest frequency of EBV detection was among 41–60 year-olds (23/42, 54.7 %), followed by 21–40 year-olds (12/42, 28.5 %) and 61–80 year-olds (5/42, 11.9 %). In the control group, the majority were diagnosed with fibroadenoma (70.4%), followed by fibrocystic changes (10.4%) and lactating changes (0.9%). Conclusion: The data obtained in this study demonstrated that EBV was present in a high percentage of our study population; however, the exact role of EBV in Sudanese breast cancer needs to be studied more in depth.

In situ detection of Epstein–Barr virus in breast cancer

1998 ◽  
Vol 124 (1) ◽  
pp. 53-57 ◽  
Author(s):  
Jan-Show Chu ◽  
Chung-Chung Chen ◽  
King-Jen Chang
Keyword(s):  
Breast Cancer ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Epstein Barr ◽  
In Situ Detection

scholarly journals Lack of association between Epstein–Barr virus and mammary tumours in dogs

2018 ◽  
Vol 62 (3) ◽  
pp. 309-315
Author(s):  
Gustavo A. Roa López ◽  
Jhon Jairo Suárez ◽  
Paola Barato ◽  
Noel Verján García
Keyword(s):  
Breast Cancer ◽  
Mammary Gland ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Mammary Tumours ◽  
Mammary Gland Cancer ◽  
Epstein Barr ◽  
And Control ◽  
First Time

AbstractIntroductionEpstein–Barr virus (EBV) is a γ-herpesvirus associated with various neoplasms in humans and is a probable aetiological agent in breast cancer; however, a causal relationship has not yet been established. Because of the epidemiological and clinicopathological similarities between breast cancer and canine mammary tumours, dogs have been proposed as a valid model for breast cancer.Material and MethodsA total of 47 canine mammary gland tumour tissues were processed by routine histopathological technique with haematoxylin-eosin staining and classified according to the type of neoplasm. DNA was extracted from paraffin-embedded tissues and the EBNA-1 gene and the BamHI-W region specific for EBV were evaluated by nested PCR.ResultsThe histopathological evaluation revealed 2 benign neoplasms, and many carcinomas: 2 in situ, 9 simple, 3 solid, 10 complex, and 21 mixed. One sample was positive for the EBNA-1 gene, while all were negative for the BamHI-W region.ConclusionNo association was found between EBV and mammary tumours in dogs. However, here we report for the first time the presence of an EBV gene sequence in a canine mammary tumour. It is likely that detection of EBV might be affected by the quality and quantity of DNA extracted from paraffin-embedded tissues. Additional studies are necessary to establish any association of EBV with mammary gland cancer in humans and in dogs, which could eventually lead to better public health prevention and control.

scholarly journals Virus–host interactions in carcinogenesis of Epstein-Barr virus-associated gastric carcinoma: Potential roles of lost ARID1A expression in its early stage

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0256440
Author(s):  
Hiroyuki Abe ◽  
Akiko Kunita ◽  
Yuya Otake ◽  
Teru Kanda ◽  
Atsushi Kaneda ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Epithelial Cells ◽  
Gastric Carcinoma ◽  
Epstein Barr Virus ◽  
Cancer Tissue ◽  
Host Interactions ◽  
Barr Virus ◽  
Epstein Barr

Epstein–Barr virus (EBV)-associated gastric carcinoma (EBVaGC) is a distinct molecular subtype of gastric cancer characterized by viral infection and cellular abnormalities, including loss of AT-rich interaction domain 1A (ARID1A) expression (lost ARID1A). To evaluate the significance of lost ARID1A in the development of EBVaGC, we performed in situ hybridization of EBV-encoded RNA (EBER) and immunohistochemistry of ARID1A in the non-neoplastic gastric mucosa and intramucosal cancer tissue of EBVaGC with in vitro infection analysis of ARID1A-knockdown and -knockout gastric cells. Screening of EBER by in situ hybridization revealed a frequency of approximately 0.2% EBER-positive epithelial cells in non-neoplastic gastric mucosa tissue samples. Six small foci of EBV-infected epithelial cells showed two types of histology: degenerated (n = 3) and metaplastic (n = 3) epithelial cells. ARID1A was lost in the former type. In intramucosal EBVaGC, there were ARID1A-lost (n = 5) and -preserved tumors (n = 7), suggesting that ARID1A-lost carcinomas are derived from ARID1A-lost precursor cells in the non-neoplastic mucosa. Lost ARID1A was also observed in non-neoplastic mucosa adjacent to an ARID1A-lost EBVaGC. In vitro experiments using siRNA knockdown and the CRISPR/Cas9-knockout system demonstrated that transient reduction or permanent loss of ARID1A expression markedly increased the efficiency of EBV infection to stomach epithelial cells. Taken together, lost ARID1A plays a role in initiating EBV-driven carcinogenesis in stomach epithelial cells, which develop to a distinct subtype of EBVaGC within the proper mucosal layer. Lost ARID1A is one of the constituents of virus–host interactions in the carcinogenesis of EBVaGC.

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