scholarly journals Molecular Epidemiology of Epstein-Barr Virus in Women Breast Cancer in Congo Brazzaville

2021 ◽  
Vol 11 (11) ◽  
pp. 180-190
Author(s):  
Dimitry Moudiongui Mboungou Malanda ◽  
Anicet Luc Magloire Boumba ◽  
Fabien Gaël Mouamba
Keyword(s):  
Breast Cancer ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Barr Virus ◽  
Significant Difference ◽  
Ebv Dna ◽  
Epstein Barr ◽  
The Republic ◽  
Congo Brazzaville ◽  
Formalin Fixed

Introduction: the Epstein Barr virus is one of the very first oncogenic viruses to be identified as responsible for human malignancies. Its role as an etiological agent of breast cancer remains controversial, however, despite the growing molecular evidence. The aim of this study was detected the presence of EBV DNA in patients with breast cancer in the Republic of Congo. Methods: The study was conducted on 90 samples of formalin fixed and paraffin-embedded tissue blocks (FFPE) from breast cancer tissue. The immunohistochemistry technique was used to test for the expression of the LMP1 antibody and DNA was extracted from all blocks of formalin-fixed and paraffin-embedded breast cancer tissue (FFPE) to detect presence of EBV 1 DNA by real-time polymerase chain reaction (PCR). Results: EBV was detected in 12.33% (12/90) of formalin-fixed, paraffin-embedded (FFPE) breast cancer tissue blocks. All formalin-fixed, paraffin-embedded (FFPE) breast cancer tissue blocks with positive EBV DNA were high tumor grades (II and III). Overall EBV infection with clinicopathological features of breast cancer cases showed no significant difference (P>0.05). However, a statistically significant difference was observed between EBV infection and histological types (P=0.04). Conclusion: Our results provide evidence for the presence of EBV DNA in female breast cancer in Congo Brazzaville. However, this evidence is substantial but inconclusive for the involvement of viruses in the development of breast cancer. Therefore, future investigations will be needed to elucidate the exact role of EBV in breast cancer in women in the Republic of Congo. Key words: EBV, breast cancer, women, Congo Brazzaville.

Epstein–Barr virus is seldom found in mammary epithelium of breast cancer tissue using in situ molecular methods

2011 ◽  
Vol 132 (1) ◽  
pp. 267-274 ◽  
Author(s):  
Kimberly Baltzell ◽  
Gertrude C. Buehring ◽  
Savitri Krishnamurthy ◽  
Henry Kuerer ◽  
Hua Min Shen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Epstein Barr Virus ◽  
Mammary Epithelium ◽  
Breast Cancer Tissue ◽  
Molecular Methods ◽  
Cancer Tissue ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Epstein-Barr Virus (EBV) Genome and Expression in Breast Cancer Tissue: Effect of EBV Infection of Breast Cancer Cells on Resistance to Paclitaxel (Taxol)

2006 ◽  
Vol 80 (2) ◽  
pp. 845-853 ◽  
Author(s):  
Hratch Arbach ◽  
Viktor Viglasky ◽  
Florence Lefeu ◽  
Jean-Marc Guinebretière ◽  
Vanessa Ramirez ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Breast Cancer Cells ◽  
Epstein Barr Virus ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr

ABSTRACT The Epstein-Barr virus (EBV) has been detected in subsets of breast cancers. In order to elaborate on these observations, we quantified by real-time PCR (Q-PCR) the EBV genome in biopsy specimens of breast cancer tissue as well as in tumor cells isolated by microdissection. Our findings show that EBV genomes can be detected by Q-PCR in about half of tumor specimens, usually in low copy numbers. However, we also found that the viral load is highly variable from tumor to tumor. Moreover, EBV genomes are heterogeneously distributed in morphologically identical tumor cells, with some clusters of isolated tumor cells containing relatively high genome numbers while other tumor cells isolated from the same specimen may be negative for EBV DNA. Using reverse transcription-PCR, we detected EBV gene transcripts: EBNA-1 in almost all of the EBV-positive tumors and RNA of the EBV oncoprotein LMP-1 in a smaller subset of the tissues analyzed. Moreover, BARF-1 RNA was detected in half of the cases studied. Furthermore, we observed that in vitro EBV infection of breast carcinoma cells confers resistance to paclitaxel (taxol) and provokes overexpression of a multidrug resistance gene (MDR1). Consequently, even if a small number of breast cancer cells are EBV infected, the impact of EBV infection on the efficiency of anticancer treatment might be of importance.

scholarly journals Prevalence of Epstein Barr Virus in biopsy specimens of nasopharyngeal carcinoma from Serbian patients

2014 ◽  
Vol 66 (2) ◽  
pp. 537-544 ◽  
Author(s):  
Ana Banko ◽  
Ivana Lazarevic ◽  
M. Folic ◽  
Maja Cupic ◽  
Tanja Jovanovic
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Biopsy Specimens ◽  
C Terminus ◽  
Geographical Regions ◽  
Significant Difference ◽  
Ebv Dna ◽  
Epstein Barr

The development of nasopharyngeal carcinoma (NPC) is the result of interaction between Epstein-Barr Virus (EBV) and many non-viral factors. The aims of this study were to determine the prevalence of EBV in NPC biopsies from Serbian patients and to investigate the correlation between EBV presence and demographic, anamnestic and clinical data. Ninety-three tissue blocks were included. For detection of EBV DNA, the C terminus of the LMP1 gene was amplified by nested-PCR. Twenty-eight biopsies were EBV-DNA-positive (30.1%), with a statistically significant difference in EBV DNA presence between geographical regions (p=0.02) and between the stages of tumor-node-metastasis (TNM) (p=0.02). A correlation was also found with the presence of EBV DNA and smoking (p=0.02). The correlation of EBV DNA presence, with or without smoking and the promising outcome of the disease was statistically significant (p=0.02; p=0.01). The EBV DNA findings from this study confirm the role of EBV in NPC carcinogenesis, and show the different distribution among TNM stages and correlation between the virus and outcome of disease.

scholarly journals Molecular detection of Epstein-Barr virus in breast cancer among Sudanese female population: a case-control study

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 1355
Author(s):  
Eiman S. Ahmed ◽  
Lubna S. Elnour ◽  
Emmanuel E. Siddig ◽  
Rowa Hassan
Keyword(s):  
Breast Cancer ◽  
Epstein Barr Virus ◽  
Case Control Study ◽  
Case Control ◽  
Control Group ◽  
Cancer Tissue ◽  
Female Population ◽  
Barr Virus ◽  
Epstein Barr ◽  
Control Study

Background: Breast cancer is the most common cancer in women worldwide and in Sudan. Breast cancer occurs due to a multifactorial process and infection with an oncogenic virus has been recently investigated as a possible risk factor for breast cancer. For nearly two decades, studies have incriminated Epstein-Barr virus (EBV) in the etiology of breast cancer. However, the results are unconvincing, and their interpretation has remained a matter of debate. The aim of this study was to detect EBV in breast cancer biopsies obtained from Sudanese female patients. Methods: A descriptive, hospital-based, case-control study, conducted at Faculty of Medical Laboratory Science, University of Khartoum, Khartoum, Sudan. Archival blocks were obtained from 115 patients with breast cancer and 115 controls during the period between November 2016 till March 2017. Results: Among 115 breast cancer tissue specimens, EBV DNA was identified in 42/115 (36.5 %) samples and was not identified in 73/115 (63.5 %) tissue samples. The highest frequency of EBV detection was among 41–60 year-olds (23/42, 54.7 %), followed by 21–40 year-olds (12/42, 28.5 %) and 61–80 year-olds (5/42, 11.9 %). In the control group, the majority were diagnosed with fibroadenoma (70.4%), followed by fibrocystic changes (10.4%) and lactating changes (0.9%). Conclusion: The data obtained in this study demonstrated that EBV was present in a high percentage of our study population; however, the exact role of EBV in Sudanese breast cancer needs to be studied more in depth.

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