scholarly journals A combination of biomolecules enhances expression of E-cadherin and peroxisome proliferator-activated receptor gene leading to increased cell proliferation in primary human meniscal cells: an in vitro study

2015 ◽  
Vol 68 (5) ◽  
pp. 1747-1761 ◽  
Author(s):  
Mamatha M. Pillai ◽  
V. Elakkiya ◽  
J. Gopinathan ◽  
C. Sabarinath ◽  
S. Shanthakumari ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Receptor Gene ◽  
In Vitro Study ◽  
Vitro Study ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Meniscal Cells ◽  
E Cadherin

Hydroxyl Safflower Yellow Pigment A (HSYA) Improves Vascular Endothelial Injury Induced by Lipopolysaccharide In Vitro Study

2021 ◽  
Vol 11 (9) ◽  
pp. 1792-1798
Author(s):  
Li Yan ◽  
Ge Jingping ◽  
Yin Yuanyuan ◽  
Li Xiaomei ◽  
Zhao Boxiang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
In Vitro Study ◽  
Treated Group ◽  
Yellow Pigment ◽  
Endothelial Injury ◽  
Vitro Study ◽  
Vascular Endothelial ◽  
Treatment Groups ◽  
Dose Dependent

Aim: This research was to investigate the effects and mechanisms of HSYA in vascular endothelial injury by vitro study. Methods: Dividing HUVECs as Normal Control (NC), Model (LPS treated) group, HSYA-L, HSYA-M and HSYA-H groups. Cells in the HSYA treatment groups were treated with LPS, followed by 40 mg/ml, 80 mg/ml, and 120 mg/ml HSYA intervention (HSYA-L, HSYA-M, and HSYA -H groups), respectively. Measuring the cell proliferation, apoptosis, relative proteins and mRNA (TLR4, MyD88 and NF-κB(p65)) expressions by MTT, Flow cytometry, WB and RT-qPCR assay. Using cellular immunofluorescence to evaluate NF-κB(p65) nuclear volume of difference groups. Results: With HSYA supplement, the cell proliferation rates were significantly up-regulation with cell apoptosis significantly down-regulation with TLR4 relatived mRNA and proteins and NF-κB(p65) nuclear significantly depressed with dose-dependent (P <0.05, respectively). Conclusion: HSYA improved vascular endothelial injury induced by LPS via TLR4 pathway In Vitro.

scholarly journals BC nanofibres: In vitro study of genotoxicity and cell proliferation

2009 ◽  
Vol 189 (3) ◽  
pp. 235-241 ◽  
Author(s):  
Susana Moreira ◽  
Naisandra Bezerra Silva ◽  
Jailma Almeida-Lima ◽  
Hugo Alexandre Oliveira Rocha ◽  
Silvia Regina Batistuzzo Medeiros ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
In Vitro Study ◽  
Vitro Study

Dehydration inhibits matrix synthesis and cell proliferation: An in vitro study of rabbit flexor tendons

1991 ◽  
Vol 62 (2) ◽  
pp. 159-162 ◽  
Author(s):  
Sven-Olof Abrahamsson ◽  
L. Stefan Lohmander ◽  
Göran Lundborg
Keyword(s):  
Cell Proliferation ◽  
In Vitro Study ◽  
Vitro Study ◽  
Matrix Synthesis ◽  
Flexor Tendons

scholarly journals An In Vitro Study on Mitochondrial Compensatory Response Induced by Gliadin Peptides in Caco-2 Cells

2019 ◽  
Vol 20 (8) ◽  
pp. 1862 ◽  
Author(s):  
Orlando ◽  
Chimienti ◽  
Pesce ◽  
Fracasso ◽  
Lezza ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
In Vitro Study ◽  
Peroxisome Proliferator ◽  
Compensatory Response ◽  
Peroxisome Proliferator Activated Receptor ◽  
Strand Breaks ◽  
Abasic Sites ◽  
D Loop ◽  
Gliadin Peptides

Dietary gliadin may show a broad spectrum of toxicity. The interplay between mitochondria and gliadin-induced oxidative stress has not been thoroughly examined in the intestinal epithelium. In this kinetic study, Caco-2 cells were exposed for 24 h to pepsin-trypsin-digested gliadin, alone or in combination with the antioxidant 2,6-di-tbutyl-p-cresol (BHT), and the effects on mitochondrial biogenesis and mtDNA were studied. Cells ability to recover from stress was determined after 24 h and 48 h of incubation in the culture medium. Gliadin-induced oxidative stress evoked a compensatory response. The stressor triggered a rapid and significant increase of Peroxisome proliferator-activated receptor γ coactivator-1alpha (PGC-1α) and Peroxiredoxin III (PrxIII) proteins, and mtDNA amount. As for the effects of gliadin on mtDNA integrity, strand breaks, abasic sites, and modified bases were analyzed in three mtDNA regions. D-loop appeared a more fragile target than Ori-L and ND1/ND2. The temporal trend of the damage at D-loop paralleled that of the amount of mtDNA. Overall, a trend toward control values was shown 48 h after gliadin exposure. Finally, BHT was able to counteract the effects of gliadin. Results from this study highlighted the effects of gliadin-induced oxidative stress on mitochondria, providing valuable evidence that might improve the knowledge of the pathophysiology of gluten-related disorders.

Evaluation of Osteoblast Responses to Silver-Hydroxyapatite/Titania Nanoparticles In Vitro

2007 ◽  
Vol 330-332 ◽  
pp. 447-450 ◽  
Author(s):  
Jing Chao Zhang ◽  
Juan Liao ◽  
An Chun Mo ◽  
Hong Kun Wu ◽  
Yu Bao Li ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Proliferation ◽  
In Vitro Study ◽  
Vitro Study ◽  
Titania Nanoparticles ◽  
Respiration Rates ◽  
The Difference

In the present in vitro study, osteoblasts proliferation, vitality and ultrastructure were investigated when cultured in the presence of silver-hydroxyapatite/titania nanoparticles (nAg_HA/TiO2) compared to HA nanoparticles (nHA) at various concentrations and cell culture without nanoparticles for up to 120 hours. Results confirmed the detrimental influences of both nAg_HA/TiO2 and nHA on osteoblast growth.Cell vitality was slightly higher during the earlier 24h, but after that was inhibited. Both cell proliferation and vitality by addition of nanoparticles were restricted with concentrations of nanoparticles increasing. However, the respiration rates by addition of nanoparticles were showed higher than that of the cell culture without nanoparticles. No remarkable ultrastructure changes were showed in the osteoblasts exposed nanoparticles. The difference in cell proliferation, vitality and ultrastructure between nAg_HA/TiO2 and nHA were insignificant. It was demonstrated that biocompatibility of nAg_HA/TiO2 is almost the same as nHA.

Aerosol vehicle for Delivery of Epidermal Cells – An in Vitro Study

1997 ◽  
Vol 5 (3) ◽  
pp. 153-156 ◽  
Author(s):  
Andre Bahoric ◽  
A Robertson Harrop ◽  
Howard M Clarke ◽  
Ronald M Zuker
Keyword(s):  
Cell Culture ◽  
Cell Proliferation ◽  
In Vitro Study ◽  
Daily Basis ◽  
Epidermal Cells ◽  
Vitro Study ◽  
Full Thickness ◽  
Thickness Skin ◽  
Skin Biopsies

This in vitro study investigated the possibility of delivering epidermal cells to cell culture plates using a simple and inexpensive aerosolization apparatus. Full-thickness skin biopsies were incubated in dispase to separate the epidermis from the dermis, and the epidermis was treated with trypsin to separate the epidermal cells from one another. The cells were suspended in aerosolization medium (1x106 cells/mL) and sprayed onto culture plates using a sterilized pump-action aerosol nozzle. The plates were incubated and microscopically examined on a daily basis. The aerosolization process was successful in consistently delivering a uniform distribution of suspended epidermal cells. By day 4 there was evidence of cell proliferation, and by days 7 to 9 a confluent layer of cells was achieved on the plates. The monolayer consisted primarily of keratinocytes interspersed with a few fibroblasts. The aerosol method was shown to be effective at delivering a suspension of viable epidermal cells to a culture plate.

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