Development of extracellular matrix supported 3D culture of renal cancer cells and renal cancer stem cells

Renal cell carcinoma (RCC) is a major neoplasm with high incidence in western countries. Tumors are heterogeneous and are composed of differentiated cancer cells, stromal cells, and cancer stem cells (CSCs). CSCs possess two main properties: self-renewal and proliferation. Additionally, they can generate new tumors once transplanted into immunodeficient mice. Several approaches have been described to identify them, through the expression of cell markers, functional assays, or a combination of both. As CSCs are involved in the resistance mechanisms to radio- and chemotherapies, several new strategies have been proposed to directly target CSCs in RCC. One approach drives CSCs to differentiate into cancer cells sensitive to conventional treatments, while the other proposes to eradicate them selectively. A series of innovative therapies aiming at eliminating CSCs have been designed to treat other types of cancer and have not been experimented with on RCC yet, but they reveal themselves to be promising. In conclusion, CSCs are an important player in carcinogenesis and represent a valid target for therapy in RCC patients.

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Microvesicles Released from Human Renal Cancer Stem Cells Stimulate Angiogenesis and Formation of Lung Premetastatic Niche

Cancer Research ◽

10.1158/0008-5472.can-11-0241 ◽

2011 ◽

Vol 71 (15) ◽

pp. 5346-5356 ◽

Cited By ~ 528

Author(s):

Cristina Grange ◽

Marta Tapparo ◽

Federica Collino ◽

Loriana Vitillo ◽

Christian Damasco ◽

...

Keyword(s):

Stem Cells ◽

Cancer Stem Cells ◽

Renal Cancer ◽

Premetastatic Niche ◽

Renal Cancer Stem Cells

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MP067MICRORNA PROFILING USING NEXT-GENERATION SEQUENCING IN RENAL CANCER STEM CELLS: A NEW REGULATORY MECHANISM

Nephrology Dialysis Transplantation ◽

10.1093/ndt/gfx162.mp067 ◽

2017 ◽

Vol 32 (suppl_3) ◽

pp. iii450-iii450

Author(s):

Grazia Serino ◽

Fabio Sallustio ◽

Vanessa Galleggiante ◽

Monica Rutigliano ◽

Claudia Curci ◽

...

Keyword(s):

Stem Cells ◽

Next Generation Sequencing ◽

Cancer Stem Cells ◽

Renal Cancer ◽

Regulatory Mechanism ◽

Next Generation ◽

Renal Cancer Stem Cells ◽

Generation Sequencing

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Targeting Strategies for Renal Cancer Stem Cell Therapy

Current Pharmaceutical Design ◽

10.2174/1381612826666200318153106 ◽

2020 ◽

Vol 26 (17) ◽

pp. 1964-1978 ◽

Cited By ~ 1

Author(s):

Pengchao Fang ◽

Liuting Zhou ◽

Lee Y. Lim ◽

Hualin Fu ◽

Zhi-xiang Yuan ◽

...

Keyword(s):

Stem Cells ◽

Renal Cell Carcinoma ◽

Stem Cell ◽

Targeted Therapy ◽

Cancer Stem Cells ◽

Renal Cancer ◽

Treatment Resistance ◽

Treatment Efficiency ◽

Renal Carcinogenesis ◽

Renal Cancer Stem Cells

Renal cell carcinoma (RCC) is an intractable genitourinary malignancy that accounts for approximately 4% of adult malignancies. Currently, there is no approved targeted therapy for RCC that has yielded durable remissions, and they remain palliative in intent. Emerging evidence has indicated that renal tumorigenesis and RCC treatment-resistance may originate from renal cancer stem cells (CSCs) with tumor-initiating capacity (CSC hypothesis). A better understanding of the mechanism underlying renal CSCs will help to dissect RCC heterogeneity and drug treatment efficiency, to promote more personalized and targeted therapies. In this review, we summarized the stem cell characteristics of renal CSCs. We outlined the targeting strategies and challenges associated with developing therapies that target renal CSCs angiogenesis, immunosuppression, signaling pathways, surface biomarkers, microRNAs and nanomedicine. In conclusion, CSCs are an important role in renal carcinogenesis and represent a valid target for treatment of RCC patients.

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BMP-2 inhibits tumor-initiating ability in human renal cancer stem cells and induces bone formation

Journal of Cancer Research and Clinical Oncology ◽

10.1007/s00432-014-1883-0 ◽

2014 ◽

Vol 141 (6) ◽

pp. 1013-1024 ◽

Cited By ~ 21

Author(s):

Lin Wang ◽

Paul Park ◽

Frank La Marca ◽

Khoi D. Than ◽

Chia-Ying Lin

Keyword(s):

Stem Cells ◽

Cancer Stem Cells ◽

Bone Formation ◽

Renal Cancer ◽

Renal Cancer Stem Cells

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Differentiation Therapy: Targeting Human Renal Cancer Stem Cells with Interleukin 15

JNCI Journal of the National Cancer Institute ◽

10.1093/jnci/djr451 ◽

2011 ◽

Vol 103 (24) ◽

pp. 1884-1898 ◽

Cited By ~ 54

Author(s):

Sandy Azzi ◽

Stefania Bruno ◽

Julien Giron-Michel ◽

Denis Clay ◽

Aurore Devocelle ◽

...

Keyword(s):

Stem Cells ◽

Cancer Stem Cells ◽

Renal Cancer ◽

Differentiation Therapy ◽

Interleukin 15 ◽

Renal Cancer Stem Cells

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Proteases Regulate Cancer Stem Cell Properties and Remodel Their Microenvironment

Journal of Histochemistry & Cytochemistry ◽

10.1369/00221554211035192 ◽

2021 ◽

pp. 002215542110351

Author(s):

Anamarija Habič ◽

Metka Novak ◽

Bernarda Majc ◽

Tamara Lah Turnšek ◽

Barbara Breznik

Keyword(s):

Stem Cells ◽

Extracellular Matrix ◽

Stem Cell ◽

Brain Tumor ◽

Tumor Microenvironment ◽

Cancer Stem Cells ◽

Cancer Stem Cell ◽

Cancer Cells ◽

Proteolytic Activity ◽

Intercellular Interactions

Proteolytic activity is perturbed in tumors and their microenvironment, and proteases also affect cancer stem cells (CSCs). CSCs are the therapy-resistant subpopulation of cancer cells with tumor-initiating capacity that reside in specialized tumor microenvironment niches. In this review, we briefly summarize the significance of proteases in regulating CSC activities with a focus on brain tumor glioblastoma. A plethora of proteases and their inhibitors participate in CSC invasiveness and affect intercellular interactions, enhancing CSC immune, irradiation, and chemotherapy resilience. Apart from their role in degrading the extracellular matrix enabling CSC migration in and out of their niches, we review the ability of proteases to modulate CSC properties, which prevents their elimination. When designing protease-oriented therapies, the multifaceted roles of proteases should be thoroughly investigated.

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