Pathogenicity and reproductive fitness of Pratylenchus coffeae and Radopholus arabocoffeae on Arabica coffee seedlings (Coffea arabica cv. Catimor) in Vietnam

AbstractPratylenchus coffeae is a parasitic nematoda that infected the roots of some plants, one of them is coffee. The Infection of Pratylenchus coffeae cause root tissue damage that led to root lession and make root become rotten, it will interfere the ability of roots to absorb water and nutrients in the soil which resulted in the growth of plants. At the moment, control of Pratylenchus coffeae are following integrated pests management (IPM) program, which integrated between the use of coffee resistant clone and application of biological agents. Research on biological control was conducted more intensive, at the moment; due to it is friendlier save against environment and cheaper then using chemical nematicides. The research was conducted to know the effects of Micorrhiza Helper Bacteria (MHB),Pseudomonas diminuta and Bacillus subtilis in suppressing the population of P. coffeaeas well as their effect on growth of coffee seedling. Coffee arabica (Coffea arabica L.) seedling one moth old were used in the experiment. The experiment prepared with eight treatments and five replications, as follows: A (Pseudomonas diminuta with density of 108 cfu / ml), B (Pseudomonas diminuta with density of 2x108 cfu / ml), C (Bacillus subtilis with density of 108 cfu / ml ), D (Bacillus subtilis with density 2x108 cfu / ml), E (Carbofuran nematicide 5 g formulation / pot), F (Pseudomonas diminuta and Bacillus subtilis with each density of 108 cfu / ml), K- (Nematoda inoculation but without bacteria and nematicide), K+ (coffee seedling without any additional treatment). The experiment was conducted for sixteen weeks or about four months. The results of the experiment showed that application of MHB could suppress population of P. coffeae and increase coffee seedling growth significantly. Inoculation of B. subtilis at 108 cfu per seedling suppressed significantly nematoda population of 71.3% compared with untreated seedling but inoculated with nematoda. It was not significant different with carbofuran treatment which could suppress nematoda population by 89.7%. The same result also occur on the treatment of P. diminuta at density level 2.108 cfu/seedling, which could suppress nematoda population by 64.2%. Seedling growth were treated with MHB also significantly increase compared with seedling without treatment and inoculation of nematodas, especially on the treatment of B.subtilis at density level 108 cfu and P.diminuta at density level of 108 cfu, with increasing level of 35.4% and 34.2 %, respectively.Keywords: MHB (Mycorrhiza Helper Bacteria), Arabica coffee (Coffea arabica L.), Pratylenchus coffeae, Bacillus subtilis, Pseudomonas diminuta.

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Decline of Pratylenchus coffeae and Radopholus arabocoffeae populations after death and removal of 5-year-old arabica coffee (Coffea arabica cv. Catimor) trees

Nematology ◽

10.1163/138855410x528505 ◽

2011 ◽

Vol 13 (4) ◽

pp. 491-500 ◽

Cited By ~ 5

Author(s):

Chau Nguyen ◽

Maurice Moens ◽

Wim Wesemael ◽

Phap Trinh

Keyword(s):

Coffea Arabica ◽

Nematode Species ◽

Soil Samples ◽

Control Measures ◽

Parasitic Nematodes ◽

Plant Parasitic Nematodes ◽

Arabica Coffee ◽

Soil Layers ◽

Pratylenchus Coffeae ◽

Coffee Plants

AbstractThe decline of populations of the plant-parasitic nematodes Radopholus arabocoffeae and Pratylenchus coffeae was studied after the death and removal of severely damaged coffee plants (Coffea arabica cv. Catimor) in a field naturally infested with both nematodes. Soil samples were taken at monthly intervals up to a depth of 70 cm and divided into 10 cm segments from which nematodes were extracted. The numbers of R. arabocoffeae and P. coffeae in soil and in root fragments were determined. Both species were found up to 70 cm depth. Densities of R. arabocoffeae and P. coffeae populations declined exponentially over time in all soil layers. No nematodes were found in soil and root fragments 10 and 11 months after removal of the infected coffee plants for R. arabocoffeae and P. coffeae, respectively. Arabica coffee seedlings were planted in soil collected from the experimental plots after these time periods. The seedlings were grown for 3 months in a glasshouse and then roots and soil were analysed for presence of both nematode species. No R. arabocoffeae and P. coffeae were found, confirming that the population in the field had completely declined. The results of this experiment demonstrate that a replant of coffee is possible 1 year after removal of infected plants from a plantation without any further (chemical) control measures.

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The effect of auxin 2,4-D and cytokinin 2-ip on direct somatic embryogenesis formation of Coffea arabica L. leaf explant

Pelita Perkebunan (a Coffee and Cocoa Research Journal) ◽

10.22302/iccri.jur.pelitaperkebunan.v27i2.146 ◽

2011 ◽

Vol 27 (2) ◽

Author(s):

Rina Arimarsetiowati

Keyword(s):

Tissue Culture ◽

Somatic Embryogenesis ◽

Coffea Arabica ◽

Callus Formation ◽

Culture Technique ◽

Plant Production ◽

Direct Somatic Embryogenesis ◽

Arabica Coffee ◽

Randomized Design ◽

Coffea Arabica L

One of the propagation technique for coffee plant production is tissue culture. Tissue culture technique for Coffea arabica L. faces some problems, mainly in the planlet formation regenerated from explants. The objective of this experiment was to examine the effect 2,4-D and 2-ip combination on the formation of direct somatic embryogenesis of Coffea arabica L. in leaves explant. Auxin (2,4-D) and cytokinin (2-ip) concentrations of, respectively, 1; 5 µM and 5; 10; 15; 20 were used as treatments. This research was conducted using completely randomized design with 10 replications. Observation to induce somatic embryos was done by quantitatively on number of callus from explant and number of embryogenic callus. Beside that, observation by qualitative descriptive was also done on deve lopment of embryogenesis. The results showed that Arabica coffee leaves explant of AS 2K clones could be induced in all medium combination except 5µM 2,4-D and 20µM 2-ip combination. Arabica coffee leaves explant of S 795, Sigararutang and AS 1 varieties could be induced in all medium combination. The highest frequency of callus formation was found in AS 2K, Sigararutang and AS 1 varieties on medium containing 1µM 2,4-D in combination with 10µM 2-ip, whereas for the S 795 variety on medium containing 5µM 2,4-D in combination with 10µM 2-ip. The highest frequency of embriogenic callus in all Arabica coffee variety could be reached on medium containing 5µM 2,4-D in combination with 15µM 2-ip. Key words : Coffea arabica L., somatic embryogenesis, 2,4-D, 2-ip, tissue culture, leaves, callus embryogenic.

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Physical Bean Quality of Arabica Coffee (Coffea Arabica) Cultivated at High and Medium Altitude

Pelita Perkebunan (a Coffee and Cocoa Research Journal) ◽

10.22302/iccri.jur.pelitaperkebunan.v32i3.241 ◽

2016 ◽

Vol 32 (3) ◽

pp. 151-161

Author(s):

Dwi Nugroho ◽

Panjisakti Basunanda ◽

Suryadi Mw

Keyword(s):

Coffea Arabica ◽

Block Design ◽

Research Station ◽

Physical Quality ◽

Arabica Coffee ◽

Randomized Complete Block Design ◽

Before And After ◽

Coffee Cultivation ◽

Cup Quality

Reducing productivity, outbreak of insects and diseases, and decreasingin physical and cup quality are major problems for Arabica coffee cultivation inmedium altitude. The aim of this study was to investigate the effect of altitude onphysical quality in eight genotypes of Arabica coffee. This research was conductedat two locations i.e. Andungsari Research Station-Bondowoso (1250 m asl. ashigh altitude) and Kalibendo Estate-Banyuwangi (700 m asl. as medium altitude).Randomized complete block design was used with three replications. Collecteddata consisted of outturn, weight of 100 beans, shape bean normality, apparentswelling, bulk density before and after roasting. Combined analysis on the physicalquality traits of green bean showed genotype x altitude interactions on weight of100 beans, percentage of normal beans and percentage of empty bean. Altitudesignificantly influenced coffee outturn. Decreasing in altitude from 1.250 m asl.to 700 m asl. caused declining in the outturn as much 32.9%. BP 700A, K 29, K34, K 79, and K 99 were genotypes that stable to produce normal bean and emptybeans at high and medium altidute, while K 8, K 130, and SIG were genotypeswith high normal beans at high altitude but not stable. Genotype x altitude interactiondid not occur for physical quality of other variables of outturn, bulk densitybefore and after roasting, apparent swelling, percentage of round beans,percentage of elephant beans, and percentage of triage beans. Altitude showedsignificant effect on all of physical quality of bean variables.

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Organoleptic, Sensory and Biochemical Traits of Arabica Coffee and their Arabusta Hybrids

10.5772/intechopen.95520 ◽

2021 ◽

Author(s):

Kahiu Ngugi ◽

Jane Jerono Cheserek ◽

Chrispine Ogutu Omondi

Keyword(s):

Experimental Data ◽

Genetic Diversity ◽

Genetic Variation ◽

Coffea Arabica ◽

Small Scale ◽

Arabica Coffee ◽

Biochemical Compounds ◽

Small Scale Farmers ◽

Organoleptic Traits ◽

Cup Quality

Coffee as a cash crop, reduces food insecurity by providing regular incomes and is a major foreign exchange earner in more than fifty tropical countries where it is grown either as Arabica (Coffea arabica) or Robust (Coffea canepora). In Kenya which grow some Robusta but mostly Arabica coffee, the production has been declining, mainly because world coffee prices have plummeted to about 5 USD for a 650Kg of un-hulled beans per acre. The only way world prices are likely to increase and benefit the small-scale farmers, is by improving the cup quality and enabling these countries to sell their coffee in specialty markets. This review, underscores the importance of analyzing and estimating organoleptic, sensory and biochemical compounds diversity in Arabica coffee, since these are the factors that determine cup quality. In an attempt to do so, the chapter presents experimental data that analyzed various sensory and organoleptic traits of Arabica coffee and their Arabusta hybrids that proves that tremendous genetic diversity exists in coffee genotypes grown in Kenya and it is possible to utilize this genetic variation to improve cup quality.

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Teratogenic Effects of Arabica Coffee (Coffea arabica L) on Rats During the Organogenesis Period

Indonesian Journal of Pharmaceutical and Clinical Research ◽

10.32734/idjpcr.v3i2.4311 ◽

2021 ◽

Vol 3 (2) ◽

pp. 25-31

Author(s):

Reza Destri Anggi ◽

Yuandani ◽

Aminah Dalimunthe

Keyword(s):

Weight Loss ◽

Body Length ◽

Coffea Arabica ◽

Teratogenic Effect ◽

Teratogenic Effects ◽

Arabica Coffee ◽

Coffea Arabica L

The purpose of the research was to evaluate the teratogenic effect caused by theprovision of Arabica coffee solution with variations of 0.36 ml, 0.72 ml and 1.08 ml withpositive control of caffeine at a dose of 300 mg / kg bw to rats during organogenesis. Themeasurement parameters in this study are the appearance of reproduction, externalmalformations and scalal malformations. The results in this study found that theadministration of Arabica coffee solution with a volume of 1.08 ml and caffeine 300 mg /kg bb caused an abnormality in the reproductive appearance of weight loss and body length,whereas for extrenal malformation and scalal malforation no abnormalities were found ineach administration of the solution Arabica coffee and caffeine.

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Determination of the optimal conditions for mutagenesis induction in a commercial Arabica coffee variety.

10.1079/9781789249095.0034 ◽

2021 ◽

pp. 326-337

Author(s):

Andrés Gatica-Arias ◽

Jorge Rodríguez-Matamoros ◽

Ana Abdelnour-Esquivel ◽

Marta Valdez-Melara

Keyword(s):

Abiotic Stresses ◽

Coffea Arabica ◽

Genetic Improvement ◽

Abiotic Factors ◽

Zygotic Embryos ◽

Optimal Conditions ◽

Arabica Coffee ◽

Low Genetic Diversity ◽

Induced Mutagenesis

Abstract Low genetic diversity and autogamous reproduction limit genetic improvement of Coffea arabica L. As a consequence, susceptibility to biotic and abiotic stresses increases. Induced mutagenesis is an alternative strategy for increasing genetic variability and for the development of varieties tolerant or resistant to biotic and abiotic factors. In the present study, the effect of three mutagenic agents (NaN3, EMS and 60Co gamma-rays) on survival of Arabica coffee zygotic embryos was evaluated. The zygotic embryos were immersed for 10 min in a solution of NaN3 (0, 2.5, 5.0, 7.5, 10.0, 12.5, 15.0 and 20.0 mM) or for 2 h in a solution of EMS (0, 0.5, 1, 1.5, 2, 4 and 6% v/v) or irradiated with 0, 20, 40, 60, 80 or 100 Gy. As the concentration or dose of the applied mutagen increased, survival decreased. The LD50 values for sodium azide, EMS and 60Co were 12.5 mM (51.6%), 1% v/v (48.3%) and 40 Gy (50.0%), respectively. Our results indicated that coffee zygotic embryos are suitable for chemical and physical mutagenesis and this offers an alternative for the genetic improvement of agriculturally important traits in coffee.

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