Development of RAPD and ISSR derived SCAR markers linked to Xca1Bo gene conferring resistance to black rot disease in cauliflower (Brassica oleracea var. botrytis L.)

Black rot disease, caused by Xanthomonas campestris pv. campestris (Xcc), results in significant yield losses in Brassica oleracea crops worldwide. To find black rot disease-resistant cabbage lines, we carried out pathogenicity assays using the scissor-clipping method in 94 different B. oleracea lines. By comparing the lesion areas, we selected a relatively resistant line, Black rot Resistance 155 (BR155), and a highly susceptible line, SC31. We compared the two cabbage lines for the Xcc-induced expression pattern of 13 defense-related genes. Among them, the Xcc-induced expression level of PR1 and antioxidant-related genes (SOD, POD, APX, Trx H, and CHI) were more than two times higher in BR155 than SC31. Nitroblue tetrazolium (NBT) and diaminobenzidine tetrahydrochloride (DAB) staining analysis showed that BR155 accumulated less Xcc-induced reactive oxygen species (ROS) than did SC31. In addition, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays showed that BR155 had higher antioxidant activity than SC31. This study, focused on the defense responses of cabbage during the early biotrophic stage of infection, indicated that Xcc-induced ROS might play a role in black rot disease development. We suggest that non-enzymatic antioxidants are important, particularly in the early defense mechanisms of cabbage against Xcc.

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Brassica oleracea resistance-related proteins identified at an early stage of black rot disease

Physiological and Molecular Plant Pathology ◽

10.1016/j.pmpp.2018.06.002 ◽

2018 ◽

Vol 104 ◽

pp. 9-14 ◽

Cited By ~ 4

Author(s):

Daiane Gonzaga Ribeiro ◽

Gabriela Corassa Rodrigues da Cunha ◽

Cristiane dos Santos ◽

Luciano Paulino Silva ◽

Osmundo Brilhante de Oliveira Neto ◽

...

Keyword(s):

Brassica Oleracea ◽

Early Stage ◽

Black Rot ◽

Rot Disease ◽

Related Proteins

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Black Rot Disease Decreases Young Brassica oleracea Plants’ Biomass but Has No Effect in Adult Plants

Agronomy ◽

10.3390/agronomy11030569 ◽

2021 ◽

Vol 11 (3) ◽

pp. 569

Author(s):

Carmen Vega-Álvarez ◽

Marta Francisco ◽

Pilar Soengas

Keyword(s):

Brassica Oleracea ◽

Xanthomonas Campestris ◽

Developmental Stages ◽

Black Rot ◽

Dependent Manner ◽

Yield Losses ◽

Aerial Parts ◽

Rot Disease ◽

The Impact ◽

Adult Plants

Black rot disease, caused by the bacterium Xanthomonas campestris pv. campestris (Pammel) Dowson (Xcc), causes important yield losses in Brassica oleracea L. crops worldwide. In temperate areas, yield losses are mostly due to the discarding of those plants showing chlorotic and necrotic lesions, since they may be unmarketable. However, the biomass loss caused by the diversion of resources from the primary to the secondary defense metabolism could also affect the final crop yield. In this work, we have focused on studying the impact of Xcc race 1 invasion on the biomass production of young and adult B. oleracea plants. The results have shown that Xcc infection reduces biomass and photosynthesis in the aerial parts of seedlings and modifies their water percentage in a time-dependent manner. When adult plants were inoculated in the field, no effect was detected on the leaves or the biomass of marketable products. This was probably due to a better immune response when compared to seedlings. Since the first developmental stages of B. oleracea crops are especially vulnerable to Xcc, plant disease control should be increased in order to avoid yield losses of marketable products at the adult stage.

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Effect of Ozone onAspergillus nigerCausing Black Rot Disease in Onion

Ozone Science and Engineering ◽

10.1080/01919510600900035 ◽

2006 ◽

Vol 28 (5) ◽

pp. 347-350 ◽

Cited By ~ 9

Author(s):

V. R. Vijayanandraj ◽

D. Nagendra Prasad ◽

N. Mohan ◽

M. Gunasekaran

Keyword(s):

Black Rot ◽

Rot Disease

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Application of Bacillus pumilus isolates for management of black rot disease in strawberry

Egyptian Journal of Biological Pest Control ◽

10.1186/s41938-021-00371-z ◽

2021 ◽

Vol 31 (1) ◽

Author(s):

Farid Abd-El-Kareem ◽

Ibrahim E. Elshahawy ◽

Mahfouz M. M. Abd-Elgawad

Keyword(s):

Root Rot ◽

Bacillus Pumilus ◽

Field Conditions ◽

Black Rot ◽

Bacterial Isolates ◽

Black Root Rot ◽

Root Rot Disease ◽

Growth Area ◽

Rot Disease ◽

The Impact

Abstract Background Black root rot of strawberry plants caused by Rhizoctonia solani, Fusarium solani, and Pythium sp. is a serious disease in Egypt. Biocontrol agents have frequently proved to possess paramount and safe tools against many diseases. The impact of soil treatments with 3 Bacillus pumilus isolates on black root rot disease of strawberry plants caused by R. solani, F., and Pythium sp. under laboratory and field conditions was examined herein on the commonly used ‘Festival’ strawberry cultivar. To increase the bacterial adhesion and distribution on the roots, each seedling was dipped in bacterial cell suspension at 1 × 108 colony-forming units/ml of each separate bacterial isolate for 30 min then mixed with 5% Arabic gum. Results The tested B. pumilus isolates significantly reduced the growth area of these 3 fungi. The two bacterial isolates Nos. 2 and 3 reduced the growth area by more than 85.2, 83.6, and 89.0% for R. solani, F. solani, and Pythium sp., respectively. Likewise, the 3 bacterial isolates significantly (P ≤ 0.05) inhibited the disease under field conditions. Isolates Nos. 2 and 3 suppressed the disease incidence by 64.4 and 68.9% and disease severity by 65.3 and 67.3%, respectively. The fungicide Actamyl had effect similar to that of the 2 isolates. B. pumilus isolates significantly enhanced growth parameters and yields of strawberry plants; isolates Nos. 2 and 3 raised the yield by 66.7 and 73.3%, respectively. Conclusions Bacillus pumilus isolates could effectively manage the black rot disease in strawberry herein. Due to the significant impact of the root rot disease on strawberry yield, B. pumilus should be further tested to manage the disease on strawberry on large scale in Egypt.

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Control of black rot disease in cabbage by integration of mulching, pruning and hot water treatment of seeds

Plant Pathology & Quarantine ◽

10.5943/ppq/9/1/3 ◽

2019 ◽

Vol 9 (1) ◽

pp. 23-29

Author(s):

GJ Ombuna

Keyword(s):

Water Treatment ◽

Hot Water ◽

Black Rot ◽

Hot Water Treatment ◽

Rot Disease

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Identification of NBS-encoding genes linked to black rot resistance in cabbage (Brassica oleracea var. capitata)

Molecular Biology Reports ◽

10.1007/s11033-018-4217-5 ◽

2018 ◽

Vol 45 (5) ◽

pp. 773-785 ◽

Cited By ~ 11

Author(s):

Khandker Shazia Afrin ◽

Md Abdur Rahim ◽

Jong-In Park ◽

Sathishkumar Natarajan ◽

Hoy-Taek Kim ◽

...

Keyword(s):

Brassica Oleracea ◽

Black Rot ◽

Encoding Genes

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Glucosinolate Profile and Glucosinolate Biosynthesis and Breakdown Gene Expression Manifested by Black Rot Disease Infection in Cabbage

Plants ◽

10.3390/plants9091121 ◽

2020 ◽

Vol 9 (9) ◽

pp. 1121

Author(s):

Mehede Hassan Rubel ◽

Md. Abuyusuf ◽

Ujjal Kumar Nath ◽

Arif Hasan Khan Robin ◽

Hee Jeong Jung ◽

...

Keyword(s):

Gene Expression ◽

Xanthomonas Campestris ◽

Defense Mechanism ◽

Expression Patterns ◽

Functional Characterization ◽

Brassica Species ◽

Black Rot ◽

Clear Understanding ◽

Knock Out ◽

Rot Disease

Cabbage (Brassica oleracea var. capitata) is an economically important crop in the family Brassicaceae. Black rot disease is a top ranked cabbage disease, which is caused by Xanthomonas campestris pv. campestris (Xcc) and may reduce 50% crop loss. Therefore, we need a clear understanding of black rot disease resistance for sustainable disease management. The secondary metabolites, like Glucosinolate (GSL) presents in Brassica species, which plays a potential role in the defense mechanism against pathogens. However, there is little known about GSL-regulated resistance mechanisms and GSL biosynthesis and the breakdown related gene expression after black rot disease infection in cabbage. In this study, relative expression of 43 biosynthetic and breakdown related GSLs were estimated in the black rot resistant and susceptible cabbage lines after Xcc inoculation. Ten different types of GSL from both aliphatic and indolic groups were identified in the contrasting cabbage lines by HPLC analysis, which included six aliphatic and four indolic compounds. In the resistant line, nine genes (MYB122-Bol026204, MYB34-Bol017062, AOP2-Bo9g006240, ST5c-Bol030757, CYP81F1-Bol017376, CYP81F2-Bol012237, CYP81F4-Bol032712, CYP81F4-Bol032714 and PEN2-Bol030092) showed consistent expression patterns. Pearson’s correlation coefficient showed positive and significant association between aliphatic GSL compounds and expression values of ST5c-Bol030757 and AOP2-Bo9g006240 genes as well as between indolic GSL compounds and the expression of MYB34-Bol017062, MYB122-Bol026204, CYP81F2-Bol012237, CYP81F4-Bol032712 and CYP81F4-Bol032714 genes. This study helps in understanding the role of GSL biosynthesis and breakdown related genes for resistance against black rot pathogen in cabbage, which could be further confirmed through functional characterization either by overexpression or knock-out mutation.

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