Fresh embryo transfer versus freeze-only after in vitro maturation with a pre-maturation step in women with high antral follicle count: a randomized controlled pilot study

IntroductionIn vitro maturation (IVM) is a potential alternative to conventional in vitro fertilisation (IVF) to avoid ovarian hyperstimulation syndrome (OHSS). This is particularly relevant in women with a high antral follicle count (AFC) and/or polycystic ovary syndrome (PCOS), who are at increased risk for OHSS. However, no randomised controlled trials of IVM versus IVF in women with high AFC have reported both pregnancy and OHSS rates. The aim of this study is to compare the effectiveness and safety of one IVM cycle and one IVF with segmentation cycle within women with PCOS or high AFC-related subfertility.Methods and analysisThis randomised controlled trial will be conducted at a specialist IVF centre in Vietnam. Eligible subfertile women with PCOS and/or high AFC will be randomised to undergo either IVM or IVF. The primary outcome is live birth after the first embryo transfer of the started treatment cycle. Cycles in which no embryo is available for transfer will be considered as failures. The study has a non-inferiority design, with a maximal acceptable between-group difference of 5%. Rates of OHSS will also be reported.Ethics and disseminationEthical approval was obtained from the participating centre, and informed patient consent was obtained before study enrolment. Results of the study will be submitted for publication in a peer-reviewed journal.Trial registration numberNCT03405701; Pre-results.

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Blastocyst biopsy with comprehensive chromosome screening and fresh embryo transfer significantly increases in vitro fertilization implantation and delivery rates: a randomized controlled trial

Fertility and Sterility ◽

10.1016/j.fertnstert.2013.04.035 ◽

2013 ◽

Vol 100 (3) ◽

pp. 697-703 ◽

Cited By ~ 359

Author(s):

Richard T. Scott ◽

Kathleen M. Upham ◽

Eric J. Forman ◽

Kathleen H. Hong ◽

Katherine L. Scott ◽

...

Keyword(s):

Randomized Controlled Trial ◽

In Vitro Fertilization ◽

Embryo Transfer ◽

Controlled Trial ◽

Fresh Embryo Transfer ◽

Vitro Fertilization ◽

Randomized Controlled ◽

Comprehensive Chromosome Screening ◽

Blastocyst Biopsy

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Plasma anti-Müllerian hormone concentration as a predictive endocrine marker for selection of donor lambs to improve success in juvenile in vitro embryo transfer programs

Reproduction Fertility and Development ◽

10.1071/rd18498 ◽

2020 ◽

Vol 32 (4) ◽

pp. 383 ◽

Cited By ~ 2

Author(s):

Hayley McGrice ◽

Jennifer M. Kelly ◽

David O. Kleemann ◽

Karen L. Kind ◽

Alissa J. Hampton ◽

...

Keyword(s):

Embryo Transfer ◽

Ovarian Response ◽

Antral Follicle ◽

Antral Follicle Count ◽

Hormone Concentration ◽

Transfer Programs ◽

Antral Follicles ◽

Selection Of

The use of juvenile invitro embryo transfer (JIVET) is limited by variation between prepubertal lambs in ovarian response to exogenous gonadotrophins. In cattle, anti-Müllerian hormone (AMH) is a predictive endocrine marker of antral follicle count. In this study we measured plasma AMH concentrations in lambs at 3 and 5 weeks of age and determined associations between AMH concentrations and ovarian response to gonadotrophins and invitro blastocyst production at 6–8 weeks of age in a JIVET program. At 5 weeks, AMH (n=38) was positively correlated with surface antral follicle count (r=0.87, P<0.001), blastocysts produced (r=0.92, P<0.001) and blastocysts produced as a proportion of oocytes collected (r=0.44, P<0.01) or cleaved (r=0.43, P<0.01). Similar associations were observed between AMH at 3 weeks (n=30) and follicle number (r=0.70, P<0.05) and blastocysts produced (r=0.87, P<0.05). Lambs with high (>2.2ngmL−1) compared with medium (0.4–2.2ngmL−1) and low (<0.4ngmL−1) AMH at 5 weeks had more antral follicles (mean (±s.e.m.) 118.7±13.9 vs 68.2±8.1 and 30.4±12.3 respectively; P<0.05) and more blastocysts produced (mean (±s.e.m.) 54.9±6.9 vs 18.9±4.0 and 7.5±6.1 respectively; P<0.05). These results suggest that AMH concentration at 5 weeks of age can be used to select donor lambs which enhance the success of JIVET programs.

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Fresh versus frozen single embryo transfer in Chinese women of advanced age undergoing IVF/ICSI: a study protocol for a randomized controlled trial

10.21203/rs.3.rs-18972/v1 ◽

2020 ◽

Author(s):

Miaoxin Chen ◽

Yuanyuan Wu ◽

Xin Huang ◽

Wenqiang Liu ◽

Wentao Li ◽

...

Keyword(s):

Embryo Transfer ◽

Live Birth ◽

Birth Rate ◽

Controlled Trial ◽

Live Birth Rate ◽

Advanced Age ◽

Cumulative Live Birth Rate ◽

Fresh Embryo Transfer ◽

Randomized Controlled

Abstract Background: Elective frozen cleavage embryo transfer resulted in significantly higher live birth rate in patients with polycystic ovary syndrome but not in ovulatory women compared with fresh embryo transfer. Further, elective single frozen blastocyst transfer had significantly higher live birth rate in ovulatory women compared with single fresh blastocyst transfer. However, it is unknown whether single frozen cleavage embryo transfer results in higher cumulative live birth rate in women of advanced age undergoing in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). The aim of this study is to compare the effectiveness of frozen cleavage embryo transfer with fresh cleavage embryo transfer in Chinese women of advanced age. Methods: This study is a double-blind randomized controlled clinical trial (1:1 treatment ratio of frozen embryo transfer vs. fresh embryo transfer). A total of 840 women of advanced age with normal ovarian reserve undergoing the first cycle of IVF or ICSI will be enrolled and randomized into two parallel groups. Participants in group A will undergo frozen single cleavage embryo transfer, and participants in group B will undergo fresh single cleavage embryo transfer. The primary outcome is the cumulative live birth rate of the trial IVF/ICSI cycle within 12 months after randomization. This study is powered to detect an absolute difference of 8% (23% vs 15%) at the significance level of 0.05 and 80% statistical power based on a two-sided test. Discussion: The results of this study will provide evidence for the efficacy and safety of frozen cleavage embryo transfer compared with fresh cleavage embryo transfer in women of advanced age undergoing IVF/ICSI. Trial registration: Chinese Clinical Trial Registry, ChiCTR2000029330. Registered on 25 Jan 2020. Keywords: Frozen embryo transfer, advanced age, in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), live birth, randomized controlled trial.

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Hypergonadotropic hypogonadism: looking beyond ovarian senescence

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20210346 ◽

2021 ◽

Vol 10 (2) ◽

pp. 791

Author(s):

Richa Vatsa ◽

Vanita Suri ◽

Neelam Choudahary ◽

Aashima Arora ◽

Shruti Sharma

Keyword(s):

Ovarian Reserve ◽

In Vitro Maturation ◽

Elevated Serum ◽

Antral Follicle ◽

Antral Follicle Count ◽

Hypergonadotropic Hypogonadism ◽

Primary Infertility ◽

High Doses ◽

Resistant Ovary Syndrome

Gonadotropin resistant ovary syndrome (GROS) is a rare cause of primary infertility where ovarian reserve is present but they fail to respond to gonadotropin stimulation. This condition can be easily confused with premature ovarian insufficiency (POI) if thorough workup is not done as in both the cases serum FSH is high, but ovarian reserve is normal in GROS and low or absent in POI. So, we are presenting this case of GROS. A 28-year-old lady presented with oligomenorrhoea since menarche and primary infertility. On workup her serum FSH and LH levels were markedly elevated, serum estradiol was normal. Markers of ovarian reserve, ante Mullerian hormones (AMH) and antral follicle count (AFC), were normal. Her autoantibody assay was also normal. She did not respond to stimulation with high doses of gonadotropins (uHMG). Hypergonadotropic hypogonadism is not always POI. We should not miss diagnosis of GROS where it is possible to have own biological child by in vitro maturation of immature oocytes, whereas in POI donor oocyte is the only fertility option.

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Should fertile women quit drinking alcohol to produce better quality oocytes?

Zygote ◽

10.1017/s0967199420000696 ◽

2020 ◽

pp. 1-3

Author(s):

Burcu Ozbakir ◽

Pinar Tulay

Keyword(s):

Alcohol Consumption ◽

Young Women ◽

Antral Follicle ◽

Antral Follicle Count ◽

Control Group ◽

Social Drinkers ◽

Antral Follicles ◽

Healthy Women ◽

Significant Difference

Summary Alcohol consumption has long been shown to affect both fetal health and pregnancy. In this study, antral follicle count, maturation level of oocytes including morphological assessment and number of metaphase I (MI), metaphase II (MII) and germinal vesicle (GV) stage oocytes obtained from young women (age < 30 years old) with or without alcohol consumption were investigated. In total, 20 healthy women who were social drinkers and 36 healthy women who do not consume alcohol were involved in this study. Women in both study and control groups were undergoing controlled ovarian stimulation. The antral follicle count and the number and quality of the oocytes retrieved were evaluated and recorded. In total, 635 antral follicles, 1098 follicles and 1014 oocytes with 820 MII, 72 MI and 78 GV stage oocytes were collected from the social drinkers. In the control group, 628 antral follicles, 1136 follicles and 1085 oocytes with 838 MII, 93 MI and 102 GV stage oocytes were evaluated. The results of this study showed that the antral follicle count was very similar in both groups. The number of oocytes and MII stage oocytes was slightly higher in the control group, although it was not a significant difference. This study showed that although the consumption of alcohol may have adverse effects post-implantation, it may not have a solid effect during oogenesis in young women. The results of this study are especially important in clinical settings as some women who are social drinkers undergo in vitro fertilization treatments.

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