Poly(vinyl alcohol co-vinyl acetate) as a novel scaffold for mammalian cell culture and controlled drug release

2019 ◽  
Vol 54 (10) ◽  
pp. 7867-7882
Author(s):  
Francisca Villanueva-Flores ◽  
Margarita Miranda-Hernández ◽  
José O. Flores-Flores ◽  
Alberto Porras-Sanjuanico ◽  
Hailin Hu ◽  
...  
Keyword(s):  
Cell Culture ◽  
Drug Release ◽  
Vinyl Acetate ◽  
Mammalian Cell ◽  
Vinyl Alcohol ◽  
Mammalian Cell Culture ◽  
Controlled Drug Release ◽  
Poly Vinyl Alcohol ◽  
Novel Scaffold

Controlled drug release through carboxymethyl-chitosan/poly(vinyl alcohol) blend films

2007 ◽  
Vol 47 (9) ◽  
pp. 1373-1379 ◽  
Author(s):  
Ling-Chong Wang ◽  
Xi-Guang Chen ◽  
Le-Jun Yu ◽  
Pi-Wu Li
Keyword(s):  
Drug Release ◽  
Vinyl Alcohol ◽  
Controlled Drug Release ◽  
Carboxymethyl Chitosan ◽  
Poly Vinyl Alcohol ◽  
Blend Films

Miniature Bioreactors for Rapid Bioprocess Development of Mammalian Cell Culture

2012 ◽  
Vol 59 (1) ◽  
Author(s):  
Mohd Helmi Sani ◽  
Frank Baganz
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Process Development ◽  
Mammalian Cell Culture ◽  
Small Scale ◽  
Cell Culture Process ◽  
Deep Wells ◽  
Working Volume ◽  
Culture Process ◽  
And Control

At present, there are a number of commercial small scale shaken systems available on the market with instrumented controllable microbioreactors such as Micro–24 Microreactor System (Pall Corporation, Port Washington, NY) and M2P Biolector, (M2P Labs GmbH, Aachen, Germany). The Micro–24 system is basically an orbital shaken 24–well plate that operates at working volume 3 – 7 mL with 24 independent reactors (deep wells, shaken and sparged) running simultaneously. Each reactor is designed as single use reactor that has the ability to continuously monitor and control the pH, DO and temperature. The reactor aeration is supplied by sparging air from gas feeds that can be controlled individually. Furthermore, pH can be controlled by gas sparging using either dilute ammonia or carbon dioxide directly into the culture medium through a membrane at the bottom of each reactor. Chen et al., (2009) evaluated the Micro–24 system for the mammalian cell culture process development and found the Micro–24 system is suitable as scaledown tool for cell culture application. The result showed that intra-well reproducibility, cell growth, metabolites profiles and protein titres were scalable with 2 L bioreactors.

scholarly journals Novel Hydrogels of Chitosan and Poly (vinyl alcohol) Reinforced with Inorganic Particles of Bioactive Glass

Polymers ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 691
Author(s):  
O. Sánchez-Aguinagalde ◽  
Ainhoa Lejardi ◽  
Emilio Meaurio ◽  
Rebeca Hernández ◽  
Carmen Mijangos ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Drug Release ◽  
Bioactive Glass ◽  
Vinyl Alcohol ◽  
Release Kinetics ◽  
The Body ◽  
Poly Vinyl Alcohol ◽  
X Ray Diffraction ◽  
Inorganic Particles ◽  
Release Studies

Chitosan (CS) and poly (vinyl alcohol) (PVA) hydrogels, a polymeric system that shows a broad potential in biomedical applications, were developed. Despite the advantages they present, their mechanical properties are insufficient to support the loads that appear on the body. Thus, it was proposed to reinforce these gels with inorganic glass particles (BG) in order to improve mechanical properties and bioactivity and to see how this reinforcement affects levofloxacin drug release kinetics. Scanning electron microscopy (SEM), X-ray diffraction (XRD), swelling tests, rheology and drug release studies characterized the resulting hydrogels. The experimental results verified the bioactivity of these gels, showed an improvement of the mechanical properties and proved that the added bioactive glass does affect the release kinetics.

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