scholarly journals Differential apoptotic response of MC3T3-E1 pre-osteoblasts to biodegradable magnesium alloys in an in vitro direct culture model

2017 ◽  
Vol 28 (10) ◽  
Author(s):  
Ehsan Bonyadi Rad ◽  
Sepideh Mostofi ◽  
Matthias Katschnig ◽  
Patrik Schmutz ◽  
Magdalena Pawelkiewicz ◽  
...  
Keyword(s):  
Magnesium Alloys ◽  
Apoptotic Response ◽  
Culture Model ◽  
Biodegradable Magnesium

Growth, in vitro biodegradation and cytocompatibility properties of nano-hydroxyapatite coatings on biodegradable magnesium alloys

2016 ◽  
Vol 672 ◽  
pp. 366-373 ◽  
Author(s):  
Huawei Yang ◽  
Kada Xia ◽  
Taolei Wang ◽  
Junchao Niu ◽  
Yiming Song ◽  
...  
Keyword(s):  
Magnesium Alloys ◽  
Hydroxyapatite Coatings ◽  
Biodegradable Magnesium

Biodegradable Magnesium Implant: In Vivo and In Vitro Convergence

2014 ◽  
Author(s):  
Yeoheung Yun ◽  
Yongseok Jang ◽  
Juan Wang ◽  
Zhongyun Dong ◽  
Vesselin Shanov ◽  
...  
Keyword(s):  
Magnesium Alloys ◽  
Product Formation ◽  
In Vitro Test ◽  
Biodegradable Implant ◽  
Plasma Electrolyte ◽  
Vitro Test ◽  
Plasma Electrolyte Oxidation ◽  
Biodegradable Magnesium

In recent years, magnesium alloys have emerged as possible biodegradable implant material. A fundamental understanding of the nature of magnesium corrosion and the ability to control this process in vivo is critical to advancing the case for clinical use of magnesium based biomaterials. The biodegradation of magnesium is fundamentally linked to studies of its corrosion, which is dependent on the interfacing dynamics between the material and its environment. Thus, it is required to confirm what variable differentiate the corrosion behavior between in vitro and in vivo before optimizing and standardizing of in vitro test. This study was conducted to understand the biodegradation behavior of commercial AZ31 and Mg-Zn-Ca alloys with plasma electrolyte oxidation (PEO) under various biological environments using in vivo and in vitro testing methods mimicking in vivo physiological environment. This study is focused on the effect of Zn element concentration and PEO coating for magnesium alloys, and the correlation between the in vivo and in vitro in terms of corrosion rate, types of corrosion and corrosion product formation.

scholarly journals The Functional Properties of Mg–Zn–X Biodegradable Magnesium Alloys

Materials ◽  
2020 ◽  
Vol 13 (3) ◽  
pp. 544 ◽  
Author(s):  
Dmitry Merson ◽  
Alexander Brilevsky ◽  
Pavel Myagkikh ◽  
Alexandra Tarkova ◽  
Alexei Prokhorikhin ◽  
...  
Keyword(s):  
Corrosion Resistance ◽  
Magnesium Alloys ◽  
Functional Properties ◽  
Biomedical Applications ◽  
Mechanical Performance ◽  
Chemical Compositions ◽  
Premature Failure ◽  
Inflammatory Reactions ◽  
Biodegradable Magnesium

The implantation of metallic devices in orthopaedic surgical procedures and coronary angioplasty is associated with the risk of various adverse events: (i) mechanical (premature failure), (ii) chemo-mechanical (corrosion and corrosion-fatigue degradation) and (iii) biomedical (chronic local inflammatory reactions, tissue necrosis, etc.). In this regard, the development of biodegradable implants/stents, which provide the necessary mechanical support for the healing period of the bone or the vessel wall and then are completely resorbed, has bright prospects. Magnesium alloys are the most suitable candidates for that purpose due to their superior mechanical performance, bioresorbability and biocompatibility. This article presents the results of the comparative research on several wrought biodegradable alloys, assessing their potential for biomedical applications. The Mg–Zn–X alloys with different chemical compositions and microstructures were produced using severe plastic deformation techniques. Functional properties pivotal for biomedical applications—mechanical strength, in vitro corrosion resistance and cytotoxic activity—were included in the focus of the study. Excellent mechanical performance and low cytotoxic effects are documented for all alloys with a notable exception for one of two Mg–Zn–Zr alloys. The in vitro corrosion resistance is, however, below expectations due to critical impurities, and this property has yet to be drastically improved through the cleaner materials fabrication processing before they can be considered for biomedical applications.

In Vitro Degradation and Biocompatibility of WE43, ZK60, and AZ91 Biodegradable Magnesium Alloys

2011 ◽  
Vol 287-290 ◽  
pp. 2008-2014 ◽  
Author(s):  
Cheng Long Liu ◽  
Jiang Jiang ◽  
Meng Wang ◽  
Yue Ji Wang ◽  
Paul K Chu ◽  
...  
Keyword(s):  
Magnesium Alloys ◽  
Negative Influence ◽  
Az91 Alloy ◽  
Diphenyltetrazolium Bromide ◽  
Zk60 Alloy ◽  
Biological Performance ◽  
Biodegradable Magnesium ◽  
Hank’S Solution ◽  
High Aluminum

Successful application of magnesium alloys as degradable load-bearing implants is determined by their biological performance especially degradation and corrosion behavior in the human body. Three magnesium alloys, namely WE43, ZK60, and AZ91 are investigated in this work. The invitrodegradation behavior, cytotoxicity, and genotoxicity are evaluated by corrosion tests, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and micronuclei tests, respectively. Immersion tests indicate that the ZK60 alloy has the best corrosion resistance and lowest corrosion rate in Hank’s solution, followed by AZ91 alloy and WE43 alloy in that order. The MTT results obtained from the three magnesium alloys after 7 days of immersion indicate good cellular viability. However, excessively high aluminum and magnesium concentrations have a negative influence on the genetic stability.

In vitro evaluation of biodegradable magnesium alloys containing micro-alloying additions of strontium, with and without zinc

2015 ◽  
Vol 3 (45) ◽  
pp. 8874-8883 ◽  
Author(s):  
Jun-Lan Wang ◽  
Shayanti Mukherjee ◽  
David R. Nisbet ◽  
Nick Birbilis ◽  
Xiao-Bo Chen
Keyword(s):  
Magnesium Alloys ◽  
Solid Solubility ◽  
Bone Growth ◽  
Cathodic Reaction ◽  
In Vitro Evaluation ◽  
Alloying Additions ◽  
Biodegradable Magnesium ◽  
Kinetics Of

(a) Alloying of microlevels of Sr do not promote thein vitrocathodic reaction, whilst it slightly moderates the anodic kinetics of Mg in MEM and (b) adopted microadditions of Sr around its solid solubility in a Mg implant favourable for bone growth.

Corrosion-resistant composite coatings on biodegradable magnesium alloys: In vitro studies

2016 ◽  
Vol 61 (4) ◽  
pp. 424-428 ◽  
Author(s):  
S. V. Gnedenkov ◽  
S. L. Sinebryukhov ◽  
A. V. Puz’ ◽  
D. V. Mashtalyar ◽  
D. P. Opra
Keyword(s):  
Magnesium Alloys ◽  
Composite Coatings ◽  
In Vitro Studies ◽  
Corrosion Resistant ◽  
Biodegradable Magnesium

In vitro and in vivo assessment of the effect of biodegradable magnesium alloys on osteogenesis

2021 ◽  
Author(s):  
Ding Li ◽  
Dechuang Zhang ◽  
Qi Yuan ◽  
Lihong Liu ◽  
Hui Li ◽  
...  
Keyword(s):  
Magnesium Alloys ◽  
Biodegradable Magnesium ◽  
In Vivo Assessment

1120: Citrate and Vitamin E Blunt the SWL-Induced Free Radical Surge in an In-Vitro MDCK Cell Culture Model

2004 ◽  
Vol 171 (4S) ◽  
pp. 295-295
Author(s):  
Fernando C. Delvecchio ◽  
Ricardo M. Brizuela ◽  
Karen J. Byer ◽  
W. Patrick Springhart ◽  
Saeed R. Khan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Free Radical ◽  
Vitamin E ◽  
Mdck Cell ◽  
Cell Culture Model ◽  
Culture Model

scholarly journals An In Vitro Cell Culture Model for Pyoverdine-Mediated Virulence

Pathogens ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 9
Author(s):  
Donghoon Kang ◽  
Natalia V. Kirienko
Keyword(s):  
Cell Culture ◽  
Virulence Factors ◽  
Model Organisms ◽  
Cell Culture Model ◽  
Chronic Infections ◽  
In Vitro Cell Culture ◽  
Mitochondrial Homeostasis ◽  
Culture Model ◽  
Wide Range

Pseudomonas aeruginosa is a multidrug-resistant, opportunistic pathogen that utilizes a wide-range of virulence factors to cause acute, life-threatening infections in immunocompromised patients, especially those in intensive care units. It also causes debilitating chronic infections that shorten lives and worsen the quality of life for cystic fibrosis patients. One of the key virulence factors in P. aeruginosa is the siderophore pyoverdine, which provides the pathogen with iron during infection, regulates the production of secreted toxins, and disrupts host iron and mitochondrial homeostasis. These roles have been characterized in model organisms such as Caenorhabditis elegans and mice. However, an intermediary system, using cell culture to investigate the activity of this siderophore has been absent. In this report, we describe such a system, using murine macrophages treated with pyoverdine. We demonstrate that pyoverdine-rich filtrates from P. aeruginosa exhibit substantial cytotoxicity, and that the inhibition of pyoverdine production (genetic or chemical) is sufficient to mitigate virulence. Furthermore, consistent with previous observations made in C. elegans, pyoverdine translocates into cells and disrupts host mitochondrial homeostasis. Most importantly, we observe a strong correlation between pyoverdine production and virulence in P. aeruginosa clinical isolates, confirming pyoverdine’s value as a promising target for therapeutic intervention. This in vitro cell culture model will allow rapid validation of pyoverdine antivirulents in a simple but physiologically relevant manner.

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