scholarly journals Sol–gel based synthesis and biological properties of zinc integrated nano bioglass ceramics for bone tissue regeneration

2021 ◽  
Vol 32 (1) ◽  
Author(s):  
Pragyan Paramita ◽  
Murugesan Ramachandran ◽  
Srinivasan Narashiman ◽  
Selvamurugan Nagarajan ◽  
Dinesh Kumar Sukumar ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Bacterial Infections ◽  
Bone Repair ◽  
Sol Gel ◽  
Bone Tissue Regeneration ◽  
Osteogenic Potential ◽  
Tissue Engineering Application ◽  
Graft Material ◽  
Self Healing ◽  
Hydroxy Apatite

AbstractBone is a flexible and electro active tissue that is vulnerable to various traumatic injuries. The self-healing of damaged bone tissue towards reconstruction is limited due to the lack of proper niche compliances. Nevertheless, the classical grafting techniques like autograft/allograft for bone repair pose challenges like bacterial infections and donor-site morbidity with unsatisfactory outcomes. The use of appropriate biomaterial with osteogenic potential can meet these challenges. In this regard, bioactive glass ceramics is widely used as a bone filler or graft material because of its bonding affinity to bone leading towards bone reconstruction applications without the challenge of post implant infections. Hence, the current study is aimed at addressing this potentiality of zinc (Zn) for doped the bioglass at nano-scale advantages for bone tissue repair. Since, Zn has been demonstrated to have not only antibacterial property but also the stimulatory effect on osteoblasts differentiation, mineralization by enhancing the osteogenic genes expression. In view of these, the present study is focused on sol–gel synthesis and pysico-chemical characterization of Zinc-doped bioglass nanoparticles (Zn-nBGC) and also analyzing its biological implications. The surface morphological and physiochemical characterizations using SEM, EDX, FT-IR and XRD analysis has shown the increased surface area of Zn-nBGC particles providing a great platform for biomolecular interaction, cytocompatibility, cell proliferation and osteogenic differentiation. The obtaining hydroxy apatite groups have initiated in vitro mineralization towards osteogenic lineage formation. Zn has not only involved in enhancing cellular actions but also strengthen the ceramic nanoparticles towards antibacterial application. Hence the finding suggests a biomaterial synthesis of better biomaterial for bone tissue engineering application by preventing post-operative bacterial infection.

scholarly journals Poly (Glycerol Sebacate)-Based Bio-Artificial Multiporous Matrix for Bone Regeneration

2020 ◽  
Vol 8 ◽  
Author(s):  
Bo Liang ◽  
Qiang Shi ◽  
Jia Xu ◽  
Yi-Min Chai ◽  
Jian-Guang Xu
Keyword(s):  
Bone Tissue ◽  
Bone Repair ◽  
Bone Tissue Regeneration ◽  
Osteogenic Potential ◽  
Allogeneic Bone ◽  
Material Synthesis ◽  
Native Bone ◽  
High Elasticity ◽  
Good Biocompatibility ◽  
Endothelial Growth Factor

In recent years, bone repair biomaterials that combine cells and bioactive factors are superior to autologous and allogeneic bone implants. However, neither natural nor synthetic biomaterials can possess all desired qualities such as strength, porosity, and biological activity. In this study, we used poly (glycerol sebacate) (PGS), a synthetic material with great osteogenic potential that has attracted more attention in the field of tissue (such as bone tissue) regeneration owing to its good biocompatibility and high elasticity. It also has the advantage of being regulated by material synthesis to match the bone tissue's strength and can be easily modified to become functional. However, pure PGS lacks functional groups and hydrophilicity. Therefore, we used PGS as the substrate to graft the adhesive ligands RGD and vascular endothelial growth factor mimetic peptide. The bone repair scaffold can be prepared through photo crosslinking, as it not only improves hydrophobicity but also promotes vascularization and accelerates osteogenesis. Simultaneously, we improved the preparation method of hydrogels after freeze-drying and crosslinking to form a sponge-like structure and to easily regenerate blood vessels. In summary, a bone repair scaffold was prepared to meet the structural and biological requirements. It proved to serve as a potential bone-mimicking scaffold by enhancing tissue regenerative processes such as cell infiltration and vascularization and subsequent replacement by the native bone tissue.

scholarly journals Nanoscale Strontium-Substituted Hydroxyapatite Pastes and Gels for Bone Tissue Regeneration

Nanomaterials ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1611
Author(s):  
Caroline J. Harrison ◽  
Paul V. Hatton ◽  
Piergiorgio Gentile ◽  
Cheryl A. Miller
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Culture Media ◽  
Full Range ◽  
Sol Gel ◽  
Tissue Growth ◽  
Bone Tissue Regeneration ◽  
Tissue Culture Media ◽  
Bone Deposition

Injectable nanoscale hydroxyapatite (nHA) systems are highly promising biomaterials to address clinical needs in bone tissue regeneration, due to their excellent biocompatibility, bioinspired nature, and ability to be delivered in a minimally invasive manner. Bulk strontium-substituted hydroxyapatite (SrHA) is reported to encourage bone tissue growth by stimulating bone deposition and reducing bone resorption, but there are no detailed reports describing the preparation of a systematic substitution up to 100% at the nanoscale. The aim of this work was therefore to fabricate systematic series (0–100 atomic% Sr) of SrHA pastes and gels using two different rapid-mixing methodological approaches, wet precipitation and sol-gel. The full range of nanoscale SrHA materials were successfully prepared using both methods, with a measured substitution very close to the calculated amounts. As anticipated, the SrHA samples showed increased radiopacity, a beneficial property to aid in vivo or clinical monitoring of the material in situ over time. For indirect methods, the greatest cell viabilities were observed for the 100% substituted SrHA paste and gel, while direct viability results were most likely influenced by material disaggregation in the tissue culture media. It was concluded that nanoscale SrHAs were superior biomaterials for applications in bone surgery, due to increased radiopacity and improved biocompatibility.

scholarly journals Biomimetic and osteogenic 3D silk fibroin composite scaffolds with nano MgO and mineralized hydroxyapatite for bone regeneration

2020 ◽  
Vol 11 ◽  
pp. 204173142096779
Author(s):  
Ziquan Wu ◽  
Zhulong Meng ◽  
Qianjin Wu ◽  
Delu Zeng ◽  
Zhengdong Guo ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
Bone Repair ◽  
Composite Scaffold ◽  
Bone Tissue Regeneration ◽  
Bone Mesenchymal Stem Cells ◽  
Differentiation Potential ◽  
Composite Effect ◽  
Sd Rat

Artificial bioactive materials have received increasing attention worldwide in clinical orthopedics to repair bone defects that are caused by trauma, infections or tumors, especially dedicated to the multifunctional composite effect of materials. In this study, a weakly alkaline, biomimetic and osteogenic, three-dimensional composite scaffold (3DS) with hydroxyapatite (HAp) and nano magnesium oxide (MgO) embedded in fiber (F) of silkworm cocoon and silk fibroin (SF) is evaluated comprehensively for its bone repair potential in vivo and in vitro experiments, particularly focusing on the combined effect between HAp and MgO. Magnesium ions (Mg2+) has long been proven to promote bone tissue regeneration, and HAp is provided with osteoconductive properties. Interestingly, the weak alkaline microenvironment from MgO may also be crucial to promote Sprague-Dawley (SD) rat bone mesenchymal stem cells (BMSCs) proliferation, osteogenic differentiation and alkaline phosphatase (ALP) activities. This SF/F/HAp/nano MgO (SFFHM) 3DS with superior biocompatibility and biodegradability has better mechanical properties, BMSCs proliferation ability, osteogenic activity and differentiation potential compared with the scaffolds adding HAp or MgO alone or neither. Similarly, corresponding meaningful results are also demonstrated in a model of distal lateral femoral defect in SD rat. Therefore, we provide a promising 3D composite scaffold for promoting bone regeneration applications in bone tissue engineering.

Bioactive and Biocompatible Silica/Pseudowollastonite Aerogels

2014 ◽  
Vol 96 ◽  
pp. 21-26 ◽  
Author(s):  
P.J. Reséndiz-Hernández ◽  
D.A. Cortés-Hernández ◽  
Juan Méndez Nonell ◽  
J.C. Escobedo-Bocardo
Keyword(s):  
Bone Tissue ◽  
Ambient Pressure ◽  
Sol Gel ◽  
Ambient Pressure Drying ◽  
Bone Tissue Regeneration ◽  
Tissue Formation ◽  
Potential Applications ◽  
Ft Ir ◽  
Bone Tissue Formation

Silica aerogels have attracted increasingly more attention due to their extraordinary properties and their existing and potential applications in a wide variety of technological areas. Materials that promote bone-tissue formation at their surface and bond to osseous tissues when implanted are called bioactive, such as pseudowollastonite particles. In this work, the synthesis of aerogels with pseudowollastonite particles was performed. The synthesis involved the preparation of an alcogel by a two step sol-gel route followed by ambient pressure drying. To promote a higher bioactivity the obtained aerogels were then biomimetically treated using simulated body fluids, SBF and 1.5 SBF. A high bioactivity was demonstrated by FT-IR, SEM, EDS, and XRD. The in vitro biocompatibility was assessed by testing cytotoxicity using rat osteoblasts cultures. The results obtained indicate that these materials are highly potential aerogels for bone tissue regeneration.

scholarly journals Fabrication of High-Strength and Porous Hybrid Scaffolds Based on Nano-Hydroxyapatite and Human-Like Collagen for Bone Tissue Regeneration

Polymers ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 61 ◽  
Author(s):  
Yannan Liu ◽  
Juan Gu ◽  
Daidi Fan
Keyword(s):  
Mechanical Properties ◽  
Enzymatic Hydrolysis ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Repair ◽  
Three Dimensional ◽  
High Strength ◽  
Biological Properties ◽  
Bone Tissue Regeneration

A novel, three-dimensional, porous, human-like collagen (HLC)/nano-hydroxyapatite (n-HA) scaffold cross-linked by 1,2,7,8-diepoxyoctane (DEO) was successfully fabricated, which showed excellent mechanical and superior biological properties for bone tissue regeneration in this study. The physicochemical characterizations of different n-HA/HLC/DEO (nHD) scaffolds were investigated by determining the morphology, compression stress, elastic modulus, Young’s modulus and enzymatic hydrolysis behavior in vitro. The results demonstrated that nHD-2 and nHD-3 scaffolds showed superior mechanical properties and resistance to enzymatic hydrolysis compared to nHD-1 scaffolds. The cell viability, live cell staining and cell adhesion analysis results demonstrated that nHD-2 scaffolds exhibited low cytotoxicity and excellent cytocompatibility compared with nHD-1 and nHD-3 scaffolds. Furthermore, subcutaneous injections of nHD-2 scaffolds in rabbits produced superior anti-biodegradation effects and histocompatibility compared with injections of nHD-1 and nHD-3 scaffolds after 1, 2 and 4 weeks. In addition, the repair of bone defects in rabbits demonstrated that nHD-2 scaffolds presented an improved ability for guided bone regeneration and reconstruction compared to commercially available bone scaffold composite hydroxyapatite/collagen (HC). Collectively, the results show that nHD-2 scaffolds show promise for application in bone tissue engineering due to their excellent mechanical properties, anti-biodegradation, anti-biodegradation, biocompatibility and bone repair effects.

scholarly journals Progress and Prospects of Polymer-Based Drug Delivery Systems for Bone Tissue Regeneration

Polymers ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 2881
Author(s):  
Vyacheslav Ogay ◽  
Ellina A. Mun ◽  
Gulshakhar Kudaibergen ◽  
Murat Baidarbekov ◽  
Kuat Kassymbek ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Drug Delivery Systems ◽  
Bone Repair ◽  
Surgical Techniques ◽  
Delivery Systems ◽  
Bone Tissue Regeneration ◽  
In Vivo Studies ◽  
Natural And Synthetic

Despite the high regenerative capacity of bone tissue, there are some cases where bone repair is insufficient for a complete functional and structural recovery after damage. Current surgical techniques utilize natural and synthetic bone grafts for bone healing, as well as collagen sponges loaded with drugs. However, there are certain disadvantages associated with these techniques in clinical usage. To improve the therapeutic efficacy of bone tissue regeneration, a number of drug delivery systems based on biodegradable natural and synthetic polymers were developed and examined in in vitro and in vivo studies. Recent studies have demonstrated that biodegradable polymers play a key role in the development of innovative drug delivery systems and tissue engineered constructs, which improve the treatment and regeneration of damaged bone tissue. In this review, we discuss the most recent advances in the field of polymer-based drug delivery systems for the promotion of bone tissue regeneration and the physical-chemical modifications of polymers for controlled and sustained release of one or more drugs. In addition, special attention is given to recent developments on polymer nano- and microparticle-based drug delivery systems for bone regeneration.

scholarly journals Cellularizing hydrogel-based scaffolds to repair bone tissue: How to create a physiologically relevant micro-environment?

2017 ◽  
Vol 8 ◽  
pp. 204173141771207 ◽  
Author(s):  
Mathieu Maisani ◽  
Daniele Pezzoli ◽  
Olivier Chassande ◽  
Diego Mantovani
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
Bone Repair ◽  
Critical Issue ◽  
Bone Defects ◽  
Bone Tissue Regeneration ◽  
Differentiation Potential ◽  
Promising Alternative

Tissue engineering is a promising alternative to autografts or allografts for the regeneration of large bone defects. Cell-free biomaterials with different degrees of sophistication can be used for several therapeutic indications, to stimulate bone repair by the host tissue. However, when osteoprogenitors are not available in the damaged tissue, exogenous cells with an osteoblast differentiation potential must be provided. These cells should have the capacity to colonize the defect and to participate in the building of new bone tissue. To achieve this goal, cells must survive, remain in the defect site, eventually proliferate, and differentiate into mature osteoblasts. A critical issue for these engrafted cells is to be fed by oxygen and nutrients: the transient absence of a vascular network upon implantation is a major challenge for cells to survive in the site of implantation, and different strategies can be followed to promote cell survival under poor oxygen and nutrient supply and to promote rapid vascularization of the defect area. These strategies involve the use of scaffolds designed to create the appropriate micro-environment for cells to survive, proliferate, and differentiate in vitro and in vivo. Hydrogels are an eclectic class of materials that can be easily cellularized and provide effective, minimally invasive approaches to fill bone defects and favor bone tissue regeneration. Furthermore, by playing on their composition and processing, it is possible to obtain biocompatible systems with adequate chemical, biological, and mechanical properties. However, only a good combination of scaffold and cells, possibly with the aid of incorporated growth factors, can lead to successful results in bone regeneration. This review presents the strategies used to design cellularized hydrogel-based systems for bone regeneration, identifying the key parameters of the many different micro-environments created within hydrogels.

Transformed Cuttlefish Bone Scaffolds for Bone Tissue Engineering

2010 ◽  
Vol 89-91 ◽  
pp. 47-52 ◽  
Author(s):  
Elisa Battistella ◽  
Silvia Mele ◽  
S. Pietronave ◽  
Ismaela Foltran ◽  
G.I. Lesci ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Cell Attachment ◽  
Engineering Application ◽  
Surface Characteristics ◽  
Bone Tissue Regeneration ◽  
Tissue Engineering Application ◽  
Bone Scaffolds ◽  
Surface Development

Nature is full of many interesting things to work with, but many natural resources are also protected. In this view the recycling of aquaculture and fishery residues may lead to the manufacture of new devices and the isolation of new molecules with potential application in medicine. The aim of the present study was to explore the possibility to transform the cuttlefish bone into an hydroxyapatite scaffold suitable for bone tissue engineering application. The mixture of different lamellar porous structure of cuttlefish bone from the species Sepia Officinalis was selected and characterized, according to morphology (including porosity, surface development, surface characteristics) and mechanical properties. The material was transformed into suitable scaffold for bone tissue regeneration, trying to totally or partially convert calcium carbonate (aragonite) into calcium phosphate (hydroxyapatite HA) using hydrothermal transformation. The studies on cell attachment and proliferation (by MTT assay at different experimental times), cell morphology with Scanning Electron Microscopy (SEM), alkaline phosphatase (ALP) and osteocalcin (OC) activities and expressions by mouse osteoblast-like MC3T3-E1 cells on HA were investigated at different experimental times in cultures, in comparison with those observed on titanium specimens used as a control (ET and ST). Cell proliferation was less in HA transformed cuttlefish bone scaffolds than in ET and ST specimens. In contrast, good performance for osteoblasts differentiation was observed on HA transformed cuttlefish bone scaffolds, similar to those observed onto titanium scaffolds.

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