Identification of early response genes to salt stress in roots of melon (Cucumis melo L.) seedlings

2012 ◽  
Vol 40 (4) ◽  
pp. 2915-2926 ◽  
Author(s):  
Shiwei Wei ◽  
Linmin Wang ◽  
Yidong Zhang ◽  
Danfeng Huang
Keyword(s):  
Salt Stress ◽  
Cucumis Melo ◽  
Early Response ◽  
Early Response Genes ◽  
Cucumis Melo L

scholarly journals Transcriptome Analysis, CmMYB1 Gene Clone From Cucumis Melo L. And Its Functional Roles Under Salt Stress  

2020 ◽  
Author(s):  
Huijun Zhang ◽  
Yan Zhang ◽  
Zuyun Dai ◽  
Yushu Yuan ◽  
Shuangqin Wei
Keyword(s):  
Salt Stress ◽  
Transcriptome Analysis ◽  
Cucumis Melo ◽  
Biological Function ◽  
Early Response ◽  
Gene Clone ◽  
Wild Type ◽  
Functional Roles ◽  
Vegetative And Reproductive Growth ◽  
Cucumis Melo L

Abstract MYB TFs plays an important role in plant adaptation to abiotic stress, especially in response to salt stress. Preliminary research found that the stress resistance of the two melon varieties ‘M15’ and ‘baogua’ are quite different. To this end, we compared the transcriptomes of ‘M15’ and ‘baogua’. Transcriptome analysis found that the expression levels of 12 MYB transcription factors were significantly different between the two varieties.CmMYB1 gene was cloned from muskmelon (Cucumis melo L.), and the subcellular localization results showed that CmMYB1 was expressed in the cytoplasm and nucleus. Transform CmMYB1 gene into Arabidopsis thaliana, observe T3 phenotypes during the vegetative and reproductive growth periods.The results showed that the transgenic A. thaliana phenotype did not change significantly compared with the wild type . After treatment with 200 mM sodium chloride solution for 1 h and 3 h, the CmMYB1 expression increased significantly and began to decline after 6 h of salt stress, indicating that it functions in the early response to salt stress. These results provide a theoretical basis for the further study of the biological function of CmMYB1.

scholarly journals ÁGUA SALINA E SUBSTRATOS NO CRESCIMENTO INICIAL DO MELOEIRO

Irriga ◽  
2017 ◽  
Vol 22 (3) ◽  
pp. 469-484 ◽  
Author(s):  
Maria Ângela Casimiro Lopes ◽  
Rafael Vitor da Silveira Muniz ◽  
Samara Sibelle Vieira Alves ◽  
Aline Costa Ferreira ◽  
Francisco Vanies da Silva Sá ◽  
...  
Keyword(s):  
Salt Stress ◽  
Organic Compound ◽  
Irrigation Water ◽  
Cucumis Melo ◽  
Dry Matter ◽  
Water Salinity ◽  
Dry Matter Accumulation ◽  
Initial Growth ◽  
Irrigation Water Salinity ◽  
Cucumis Melo L

ÁGUA SALINA E SUBSTRATOS NO CRESCIMENTO INICIAL DO MELOEIRO   MARIA ÂNGELA CASIMIRO LOPES1; RAFAEL VITOR DA SILVEIRA MUNIZ1; SAMARA SIBELLE VIEIRA ALVES2; ALINE COSTA FERREIRA1; FRANCISCO VANIES DA SILVA SÁ3 E LUDERLÂNDIO DE ANDRADE SILVA3 1 Unidade Acadêmica de Ciências Agrárias, Universidade Federal de Campina Grande, UFCG, Pombal, PB, Brasil, [email protected], [email protected], [email protected] Unidade Acadêmica de Garanhuns, Universidade Federal Rural do Pernambuco, UFRPE, Garanhuns, PE, Brasil, [email protected] Centro de Ciências e Recursos Naturais, Universidade Federal de Campina Grande, UFCG, Campina Grande, PB, Brasil, [email protected], [email protected]  1 RESUMO Objetivou-se estudar a produção de mudas de meloeiro em função de diferentes substratos irrigados com água salina. O experimento foi desenvolvido em casa de vegetação no município de Pombal, Paraíba PB, no período de abril a maio de 2015. Adotou-se o delineamento experimental inteiramente casualizado, em esquema fatorial 4 x 3 x 2 constituído de quatro níveis de salinidade da água de irrigação - CEa (0,3; 1,5; 3,0 e 4,5 dS m-1) e três substratos [S1= solo; S2= solo + composto orgânico (2:1); e S3 = solo + esterco bovino (2:1)], e duas variedades de melão (V1= Melão Gaúcho Casca de Carvalho; V2= Hales Best Jumbo), com quatro repetições e três plantas úteis por repetição. As plantas foram conduzidas em recipientes com capacidade de 0,5 dm3 durante 32 dias após a semeadura, quando foram avaliadas quanto ao crescimento e acúmulo de matéria seca. O crescimento e o acúmulo de matéria seca de ambas as variedades foram reduzidos com o aumento da salinidade da água de irrigação. O crescimento e acúmulo de massa seca da variedade Hales Best Jumbo no substrato constituído de solo com composto orgânico foi menos afetado pelo estresse salino. A variedade Gaúcho Casca de Carvalho é a mais sensível ao estresse salino independente do substrato utilizado. Palavras-chave: Cucumis melo L.; estresse salino, composto orgânico.  LOPES, M. A. C.; MUNIZ, R. V. S.; ALVES, S. S. V.; FERREIRA, A. C.; SÁ, F. V. S.; ANDRADE SILVA, L. A.SALINE WATER AND SUBSTRATES ON INITIAL GROWTH IN MELON  2 ABSTRACT The aim was to study the production of melon seedlings for different substrates irrigated with saline water. The experiment was conducted in a greenhouse in the city of Pombal, Paraíba, PB, in the period April-May 2015. We adopted a completely randomized design in a factorial 4 x 3 x 2 design, consisting of four levels of irrigation water salinity (0.3, 1.5, 3.0 and 4.5 dS m-1) and three substrates [S1= ground; S2= soil + organic compound (2:1); and S3= soil + manure (2:1)] , and two varieties of melon (V1 = Gaúcho Casca de Carvalho; V2 = Hales Best Jumbo), with four replications and three plants per repetition. Plants were kept in containers with a capacity of 0.5 dm3 for 32 days after sowing, when they were evaluated for growth and accumulation of dry matter. Growth and dry matter accumulation of both varieties were reduced with increasing irrigation water salinity. The growth and accumulation of dry mass of the Hales Best Jumbo variety in the substrate composed of soil with organic compost was less affected by salt stress. The Gaúcho Casca de Carvalho variety is the most sensitive to salt stress regardless of the substrate used. Keywords: Cucumis melo L., salt stress, organic compound

scholarly journals Distinctive Traits for Drought and Salt Stress Tolerance in Melon (Cucumis melo L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Sergio Chevilly ◽  
Laura Dolz-Edo ◽  
Gema Martínez-Sánchez ◽  
Luna Morcillo ◽  
Alberto Vilagrosa ◽  
...  
Keyword(s):  
Salt Stress ◽  
Stress Tolerance ◽  
Cucumis Melo ◽  
Amino Acid Profile ◽  
Limiting Factors ◽  
Sodium Potassium ◽  
Drought And Salt Stress ◽  
Salt And Drought Stress ◽  
Cucumis Melo L ◽  
Physiological And Biochemical

Melon (Cucumis melo L.) is a crop with important agronomic interest worldwide. Because of the increase of drought and salinity in many cultivation areas as a result of anthropogenic global warming, the obtention of varieties tolerant to these conditions is a major objective for agronomical improvement. The identification of the limiting factors for stress tolerance could help to define the objectives and the traits which could be improved by classical breeding or other techniques. With this objective, we have characterized, at the physiological and biochemical levels, two different cultivars (sensitive or tolerant) of two different melon varieties (Galia and Piel de Sapo) under controlled drought or salt stress. We have performed physiological measurements, a complete amino acid profile and we have determined the sodium, potassium and hormone concentrations. This has allowed us to determine that the distinctive general trait for salt tolerance in melon are the levels of phenylalanine, histidine, proline and the Na+/K+ ratio, while the distinctive traits for drought tolerance are the hydric potential, isoleucine, glycine, phenylalanine, tryptophan, serine, and asparagine. These could be useful markers for breeding strategies or to predict which varieties are likely perform better under drought or salt stress. Our study has also allowed us to identify which metabolites and physiological traits are differentially regulated upon salt and drought stress between different varieties.

scholarly journals Comparative Analysis of Gene Expression in Two Muskmelon Cultivars (Cucumis melo L.) Under Salt Stress

2014 ◽  
Vol 13 (10) ◽  
pp. 2132-2140 ◽  
Author(s):  
Shi-wei WEI ◽  
Fu-rong ZHANG ◽  
Yi-dong ZHANG ◽  
Li-min WANG ◽  
Jia-bei CHEN ◽  
...  
Keyword(s):  
Gene Expression ◽  
Salt Stress ◽  
Comparative Analysis ◽  
Cucumis Melo ◽  
Cucumis Melo L

scholarly journals Germination and antioxidant action in melon seeds exposed to salt stress

2016 ◽  
Vol 46 (3) ◽  
pp. 336-342 ◽  
Author(s):  
Daniel Teixeira Pinheiro ◽  
Aparecida Leonir da Silva ◽  
Laércio Junio da Silva ◽  
Marcelo Coelho Sekita ◽  
Denise Cunha Fernandes dos Santos Dias
Keyword(s):  
Superoxide Dismutase ◽  
Salt Stress ◽  
Cucumis Melo ◽  
Germination Rate ◽  
Antioxidant Action ◽  
Induced Stress ◽  
Melon Seeds ◽  
Cucumis Melo L ◽  
Osmotic Potentials ◽  
Seeds Germination

ABSTRACT Salinity influences all germination stages and may adversely affect the seedlings establishment in the field. This study aimed at assessing the effects of salt stress on the physiological potential of melon (Cucumis melo L.) seeds. Germination and activity of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX) enzymes were assessed at the osmotic potentials of 0.0 MPa, -0.1 MPa, -0.2 MPa, -0.3 MPa, -0.4 MPa and -0.5 MPa. The enzymatic activity was assessed after 24 h, 48 h and 72 h of imbibition. Seed germination was zero at -0.5 MPa and 91 % and 85 % at -0.1 MPa and -0.2 MPa, respectively. In general, the activity of SOD, CAT and POX enzymes increased with the salt-induced stress, in the different times of imbibition, and these increases were more evident at -0.4 MPa and -0.5 MPa. The salt stress induced by NaCl diminishes the germination rate of melon seeds, particularly from the osmotic potential of -0.2 MPa. There is a significant increase in the activity of SOD, CAT and POX enzymes, mainly after 48 h of exposure.

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