Expression patterns of miR-221 and its target Caspase-3 in different cancer cell lines

Ovarian cancer ranks amongst the deadliest cancers in the gynaecological category of cancers. This research work aims to evaluate in vitro anti-ovarian cancer activities and identify phytochemical constituents of a rarely explored plant species—Rutidea parviflora DC. The aqueous and organic extracts of the plant were evaluated for cytotoxicity using sulforhodamine B assay in four ovarian cancer cell lines and an immortalized human ovarian epithelial (HOE) cell line. The bioactive compounds were isolated and characterized by gas/liquid chromatography mass spectrometry and nuclear magnetic resonance spectroscopy. Caspase 3/7 activity assay, western blotting and flow cytometry were carried out to assess apoptotic effects of active compounds. The extracts/fractions of R. parviflora showed promising anti-ovarian cancer activities in ovarian cancer cell lines. A principal cytotoxic alkaloid was identified as palmatine whose IC50 was determined as 5.5–7.9 µM. Palmatine was relatively selective towards cancer cells as it was less cytotoxic toward HOE cells, also demonstrating interestingly absence of cross-resistance in cisplatin-resistant A2780 cells. Palmatine further induced apoptosis by increasing caspase 3/7 activity, poly-ADP-ribose polymerase cleavage, and annexin V and propidium iodide staining in OVCAR-4 cancer cells. Our studies warranted further investigation of palmatine and R. parviflora extracts in preclinical models of ovarian cancer.

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Potent Cytotoxicity of Four Cameroonian Plant Extracts on Different Cancer Cell Lines

Pharmaceuticals ◽

10.3390/ph13110357 ◽

2020 ◽

Vol 13 (11) ◽

pp. 357

Author(s):

Ahmed Somaida ◽

Imran Tariq ◽

Ghazala Ambreen ◽

Ahmed Mohamed Abdelsalam ◽

Abdallah Mohamed Ayoub ◽

...

Keyword(s):

Cell Death ◽

Cell Migration ◽

Cell Lines ◽

Cancer Cell ◽

Plant Extracts ◽

Caspase 3 ◽

Cancer Cell Lines ◽

Imperata Cylindrica ◽

Tumor Cell Death ◽

Genetic Toxicity

In this study, the potential cytotoxicity of four plant extracts originated from Cameroon: Xylopia aethiopica (XA), Imperata cylindrica (IC), Echinops giganteus (EG) and Dorstenia psilurus (DP) were examined in vitro. We tested the anti-proliferative activity of the methanolic extracts of these compounds using MTT assay on seven different human cancer cell lines: HeLa, MDA-MB-231, A549, HepG2, U-87, SK-OV-3 and HL60. Induction of cell death was assessed by cell cycle analysis, apoptosis was determined by Annexin V-FITC binding and caspase 3/7 activity. As well, changes in mitochondrial membrane potential (MMP) and cell migration were tested. The genetic toxicity, using the alkaline comet assay, was evaluated. The studied extracts inhibited the cell proliferation of all tested cancer cell lines with concentration dependent effect over time. All of these extracts mainly induced apoptosis of HeLa cells by the accumulation of hypodiploid cells in the sub-G0/G1 phase and increasing the activity of caspase 3/7, as well they showed potential MMP disturbance and expressed a marked inhibitory effect on cell migration. Assessment of probable genetic toxicity by these extracts revealed no or minimum incidence of genetic toxicity. Therefore, the studied plant extracts are exhibiting potent anticancer activity based upon marked induction of tumor-cell death.

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Phorbol Esters from Jatropha Meal Triggered Apoptosis, Activated PKC-δ, Caspase-3 Proteins and Down-Regulated the Proto-Oncogenes in MCF-7 and HeLa Cancer Cell Lines

Molecules ◽

10.3390/molecules170910816 ◽

2012 ◽

Vol 17 (9) ◽

pp. 10816-10830 ◽

Cited By ~ 13

Author(s):

Ehsan Oskoueian ◽

Norhani Abdullah ◽

Syahida Ahmad

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Caspase 3 ◽

Phorbol Esters ◽

Cancer Cell Lines ◽

Mcf 7

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Xylopine Induces Oxidative Stress and Causes G2/M Phase Arrest, Triggering Caspase-Mediated Apoptosis by p53-Independent Pathway in HCT116 Cells

Oxidative Medicine and Cellular Longevity ◽

10.1155/2017/7126872 ◽

2017 ◽

Vol 2017 ◽

pp. 1-13 ◽

Cited By ~ 12

Author(s):

Luciano de Souza Santos ◽

Valdenizia Rodrigues Silva ◽

Leociley Rocha Alencar Menezes ◽

Milena Botelho Pereira Soares ◽

Emmanoel Vilaça Costa ◽

...

Keyword(s):

Oxidative Stress ◽

Cell Lines ◽

Cancer Cell ◽

Caspase 3 ◽

Cancer Cell Lines ◽

Apoptosis Pathway ◽

Phase Arrest ◽

M Phase ◽

Induced Apoptosis ◽

Hct116 Cells

Xylopine is an aporphine alkaloid that has cytotoxic activity to cancer cells. In this study, the underlying mechanism of xylopine cytotoxicity was assessed in human colon carcinoma HCT116 cells. Xylopine displayed potent cytotoxicity in different cancer cell lines in monolayer cultures and in a 3D model of cancer multicellular spheroids formed from HCT116 cells. Typical morphology of apoptosis, cell cycle arrest in the G2/M phase, increased internucleosomal DNA fragmentation, loss of the mitochondrial transmembrane potential, and increased phosphatidylserine externalization and caspase-3 activation were observed in xylopine-treated HCT116 cells. Moreover, pretreatment with a caspase-3 inhibitor (Z-DEVD-FMK), but not with a p53 inhibitor (cyclic pifithrin-α), reduced xylopine-induced apoptosis, indicating induction of caspase-mediated apoptosis by the p53-independent pathway. Treatment with xylopine also caused an increase in the production of reactive oxygen/nitrogen species (ROS/RNS), including hydrogen peroxide and nitric oxide, but not superoxide anion, and reduced glutathione levels were decreased in xylopine-treated HCT116 cells. Application of the antioxidant N-acetylcysteine reduced the ROS levels and xylopine-induced apoptosis, indicating activation of ROS-mediated apoptosis pathway. In conclusion, xylopine has potent cytotoxicity to different cancer cell lines and is able to induce oxidative stress and G2/M phase arrest, triggering caspase-mediated apoptosis by the p53-independent pathway in HCT116 cells.

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Expression of p120-Catenin Isoforms Correlates with Genomic and Transcriptional Phenotype of Breast Cancer Cell Lines

Analytical Cellular Pathology ◽

10.1155/2007/395913 ◽

2007 ◽

Vol 29 (6) ◽

pp. 467-476

Author(s):

Joana Paredes ◽

Ana Luísa Correia ◽

Ana Sofia Ribeiro ◽

Fernando Schmitt

Keyword(s):

Breast Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Expression Patterns ◽

Cell Signalling ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines ◽

P120 Catenin ◽

Transcriptional Phenotype

Background: P120-catenin is a member of the Armadillo protein family, which is involved in intercellular adhesion and cell signalling. It directly interacts with the classical cadherins juxtamembrane domain and contributes for both junction formation and its disassembly. Accumulating evidences indicate that p120-catenin is important in tumour formation and progression, although the role of their multiple spliced isoforms in the regulation of cadherin-mediated adhesion of malignant cells is still not well understood. We investigated the expression of p120-catenin isoforms in a collection of breast cancer cell lines with distinct molecular profiles and expressing different cadherins. Methods: We assessed the expression by RT-PCR and Western-blotting analysis. Results: We observed that the expression of p120-catenin isoforms was associated with the genomic and transcriptional phenotype of breast cancer cells. Besides, the recruitment of p120-catenin isoforms was not apparently related with the particular expression of E-, P- or N-cadherin. Conclusion: We demonstrate that mammary tumour cells exhibit a characteristic p120-catenin isoform expression profile, depending from their specific genomic and transcriptional properties. These particular expression patterns, combined with other regulatory proteins and in a specific cellular context, may explain how p120-catenin can either contribute to strength intercellular adhesions or instead to promote cell motility.

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Abstract 2045: microRNA expression patterns associated with resistance to platinum-based chemotherapy in human cancer cell lines

10.1158/1538-7445.am10-2045 ◽

2010 ◽

Author(s):

Fan-Yu He ◽

Jang-Yang Chang ◽

Ching-Chuan Kuo ◽

Wen-Yu Pan ◽

Li-Tzong Chen ◽

...

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Human Cancer ◽

Expression Patterns ◽

Cancer Cell Lines ◽

Microrna Expression ◽

Human Cancer Cell ◽

Human Cancer Cell Lines ◽

Platinum Based Chemotherapy

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Capsaicin induces apoptosis by generating reactive oxygen species and disrupting mitochondrial transmembrane potential in human colon cancer cell lines

Cellular & Molecular Biology Letters ◽

10.2478/s11658-009-0016-2 ◽

2009 ◽

Vol 14 (3) ◽

Cited By ~ 44

Author(s):

Kyung Yang ◽

Jong Pyo ◽

Gyu-Yeol Kim ◽

Rina Yu ◽

In Han ◽

...

Keyword(s):

Colon Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Caspase 3 ◽

Cancer Cell Lines ◽

Colon Cancer Cell ◽

Enzyme Treatment ◽

Dependent Manner ◽

Colon Cancer Cell Lines ◽

Induced Apoptosis

AbstractAlthough genetic factors are a well-known cause of colorectal cancer, environmental factors contribute more to its development. Despite advances in the fields of surgery, radiotherapy and chemotherapy, the cure rates for colon cancer have not substantially improved over the past few decades. Capsaicin (trans-8-methyl-N-vanillyl-6-nonenamide), the principal pungent ingredient of hot chili pepper, has exhibited an anti-tumor effect in many cell types. However, the mechanisms responsible for the anti-tumor effect of capsaicin are not yet completely understood. In this study, we investigated whether capsaicin induces apoptosis in colon cancer cell lines. Capsaicin decreased cell viability in a dose-dependent manner in Colo320DM and LoVo cells. In addition, capsaicin produced cell morphology changes and DNA fragmentation, decreased the DNA contents, and induced phosphatidylserine translocation, which is a hallmark of apoptotic cell death. We showed that capsaicin-induced apoptosis is associated with an increase in ROS generation and a disruption of the mitochondrial transmenbrane potential. A possible mechanism of capsaicin-induced apoptosis is the activation of caspase 3, a major apoptosis-executing enzyme. Treatment with capsaicin induced a dramatic increase in caspase 3 activity, as assessed by the cleavage of Ac-DEVD-AMC, a fluorogenic substrate. In conclusion, our results clearly showed that capsaicin induced apoptosis in colon cancer cells. Although the actual mechanisms of capsaicin-induced apoptosis remain uncertain, it may be a beneficial agent for colon cancer treatment and chemoprevention.

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Expression Patterns of Cancer-Testis Antigens in Human Embryonic Stem Cells and Their Cell Derivatives Indicate Lineage Tracks

Stem Cells International ◽

10.4061/2011/795239 ◽

2011 ◽

Vol 2011 ◽

pp. 1-13 ◽

Cited By ~ 27

Author(s):

Nadya Lifantseva ◽

Anna Koltsova ◽

Tatyana Krylova ◽

Tatyana Yakovleva ◽

Galina Poljanskaya ◽

...

Keyword(s):

Stem Cells ◽

Cell Lines ◽

Cancer Cell ◽

Pluripotent Stem Cells ◽

Expression Patterns ◽

Embryonic Stem ◽

Cancer Cell Lines ◽

Cancer Testis Antigens ◽

Hes Cells ◽

Cancer Testis

Pluripotent stem cells can differentiate into various lineages but undergo genetic and epigenetic changes during long-term cultivation and, therefore, require regular monitoring. The expression patterns of cancer-testis antigens (CTAs) MAGE-A2, -A3, -A4, -A6, -A8, -B2, and GAGE were examined in undifferentiated human embryonic stem (hES) cells, their differentiated derivatives, teratocarcinoma (hEC) cells, and cancer cell lines of neuroectodermal and mesodermal origin. Undifferentiated hES cells and embryoid body cells expressed MAGE-A3, -A6, -A4, -A8, and GAGEs while later differentiated derivatives expressed only MAGE-A8 or MAGE-A4. Likewise, mouse pluripotent stem cells also express CTAs of Magea but not Mageb family. Despite similarity of the hES and hEC cell expression patterns, MAGE-A2 and MAGE-B2 were detected only in hEC cells but not in hES cells. Moreover, our analysis has shown that CTAs are aberrantly expressed in cancer cell lines and display low tissue specificity. The identification of CTA expression patterns in pluripotent stem cells and their derivatives may be useful for isolation of abnormally CTA-expressing cells to improve the safety of stem-cell based therapy.

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The Expression Patterns of ER, PR, HER2, CK5/6, EGFR, Ki-67 and AR by Immunohistochemical Analysis in Breast Cancer Cell Lines

Breast Cancer Basic and Clinical Research ◽

10.1177/117822341000400004 ◽

2010 ◽

Vol 4 ◽

pp. 117822341000400 ◽

Cited By ~ 93

Author(s):

Kristina Subik ◽

Jin-Feng Lee ◽

Laurie Baxter ◽

Tamera Strzepek ◽

Dawn Costello ◽

...

Keyword(s):

Breast Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Expression Patterns ◽

Molecular Classification ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines ◽

Luminal A ◽

Ki 67

The molecular classification for breast carcinomas has been used in clinical studies with a simple surrogate panel of immunohistochemistry (IHC) markers. The objective of this current project was to study the molecular classification of commonly used breast cancer cell lines by IHC analysis. Seventeen breast cancer cell lines were harvested, fixed in formalin and made into cell blocks. IHC analyses were performed on each cell block with antibodies to estrogen receptor (ER), progesterone receptor (PR), HER2, EGFR, CK5/6, Ki-67 and androgen receptor (AR). Among the 17 cell lines, MCF-7 and ZR-75-1 fell to Luminal A subtype; BT-474 to Luminal B subtype; SKBR-3, MDA-MD-435 and AU 565 to HER2 over-expression subtype; MDA-MB-231, MCF-12A, HBL 101, HS 598 T, MCF-10A, MCF-10F, BT-20, 468 and BT-483 to basal subtype. MDA-MB-453 belonged to Unclassified subtype. Since each subtype defined by this IHC-based molecular classification does show a distinct clinical outcome, attention should be paid when choosing a cell line for any study.

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