Biological Activity of Abies Sibirica Essential Oil and its Major Constituents for Several Enzymes In Vitro

The biological activity and composition of the essential oil of the aerial flowering parts of D. moldavica have been investigated. The oil analysis was performed by GC and GC-MS. Thirteen compounds were identified, representing 99.8% of the oil. Oxygenated monoterpenes (96.3%) were found to be the principal group of compounds, of which neral, geranial, geranyl acetate and geraniol with 32.1, 21.6, 19.9 and 17.6% of the total oil were the main constituents, respectively. The in vitro antimicrobial activity of the essential oil and its main components, citral, geraniol and geranyl acetate, showed that all of the tested microorganisms were highly inhibited by the essential oil with inhibition zones ranged from 15 – 41 mm for bacteria and 29 – 30 mm for fungi. The most sensitive microorganisms were Bacillus subtilis, Staphylococcus aureus and S. epidermidis with the lowest MIC values of 0.9 mg/mL. Considering sensitivity screening, it is conceivable that the activity of the oil from D. moldavica could be attributed mainly to the presence of citral.

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Cytotoxicity of Essential Oil Cordia verbenaceae against Leishmania brasiliensis and Trypanosoma cruzi

Molecules ◽

10.3390/molecules26154485 ◽

2021 ◽

Vol 26 (15) ◽

pp. 4485

Author(s):

Pedro S. Pereira ◽

Carlos Vinicius B. Oliveira ◽

Ana J. Maia ◽

Saulo R. Tintino ◽

Cícera Datiane de M. Oliveira-Tintino ◽

...

Keyword(s):

Biological Activity ◽

Essential Oil ◽

Trypanosoma Cruzi ◽

Folk Medicine ◽

Human Consumption ◽

Shrub Species ◽

Low Concentrations ◽

Perennial Shrub ◽

Curative Agent

The species Cordia verbenacea DC (Boraginaceae), known as the whaling herb and camaradinha, is a perennial shrub species native to the Atlantic Forest. Its leaves are used in folk medicine as an anti-inflammatory, analgesic, antiulcerogenic and curative agent, in the form of teas or infusions for internal or topical use. The present study aimed to verify the cytotoxicity of the essential oil and the leishmanicidal and trypanocidal potential of C. verbenacea. The essential oil was characterized by GC-MS. The in vitro biological activity was determined by anti-Leishmania and anti-Trypanosoma assays. The cytotoxixity was determined using mammalian fibroblasts. The C. verbenacea species presented α-pinene (45.71%), β-caryophyllene (18.77%), tricyclo[2,2,1-(2.6)]heptane (12.56%) as their main compounds. The essential oil exhibited strong cytotoxicity at concentrations below 250 μg/mL (LC50 138.1 μg/mL) in mammalian fibroblasts. The potent anti-trypanosome and anti-promastigote activities occurred from the concentration of 62.5 μg/mL and was considered clinically relevant. The results also demonstrate that at low concentrations (<62.5 μg/mL), the essential oil of C. verbenacea managed to be lethal for these activities. This can be considered an indication of the power used in daily human consumption. Therefore, it can be concluded that the essential oil of C. verbenacea contains a compound with remarkable antiparasitic activities and requires further research.

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Comparative Biological Activity Profile of Nava (Freshly Collected) and Purana (Old) Dhanyaka (Coriandrum sativum Linn.) through Gas Chromatography and In-Vitro Anti-Microbial Study

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v10i3-s.4060 ◽

2020 ◽

Vol 10 (3-s) ◽

pp. 12-16

Author(s):

, Chaithra ◽

Suma V Mallya ◽

Suchitra Prabhu

Keyword(s):

Gas Chromatography ◽

Antimicrobial Activity ◽

Biological Activity ◽

Essential Oil ◽

Coriandrum Sativum ◽

Activity Profile ◽

Fresh Sample ◽

New Findings ◽

Microbial Study

Introduction: Dhanyaka(Coriandrum sativum Linn.) belonging to family Apiaceae is one such common aromatic edible herb, the fruits of which are used as carminative, antimicrobial and cooling. Classical texts of Ayurveda advocate these fruits to be used in old condition. With this background, a study was done to compare biological activity profile of Nava and Purana Dhanyaka(Coriandrum sativum Linn.) through, Gas chromatography and in-vitro anti-microbial activity. Materials and methods: Fresh fruits of Dhanyaka(Coriandrum sativum Linn.) were collected from Rona, Gadag district and authenticated. The fresh fruits named as S1 with which essential oil extracted using clevengers apparatus. Few fruits were kept in an air tight container and stored for one year, and at the end of a year named as sample(S2), essential oil extracted and stored. Both samples were subjected for GC and in-vitro antimicrobial activity. Results and Discussion: The Essential oil of Nava Dhanyaka(S1) included 17 identified compounds which was dominated by monoterpenes and sesquiterpene; whereas S2 showed 6 identified compounds in which 3 constituents isoborneol, citronellyl acetate and geraniol were new findings. In-vitro anti-microbial study of essential oil extract showed that Purana Dhanyaka(S2) had better efficacy with respect to the standard against the strain Escherichia coli and Candida albicans; whereas that of Nava Dhanyaka(S1) against only one bacterial strain ie., Streptococcus pyogens. Conclusion: Preservation of Dhanyaka(Coriandrum sativum Linn.) showed biological changes in terms of antimicrobial activity compared to fresh sample. Key words: Dhanyaka, Coriandrum sativum Linn., Gas chromatography, In-vitro antimicrobial study)

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Biological activity of some essential oil constituents in four Nepeta L. species against Sitophilus oryzae L.

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d200205 ◽

2019 ◽

Vol 20 (2) ◽

pp. 338-343 ◽

Cited By ~ 1

Author(s):

SHAHLA AMINI ◽

MAJID GHORBANI NOHOOJI ◽

MOUSA KHANI ◽

MOHAMMAD REZA LABBAFI ◽

FARAHNAZ KHALIGHI-SIGAROODI

Keyword(s):

Biological Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Sitophilus Oryzae ◽

Contact Toxicity ◽

Fumigant Toxicity ◽

Nepeta Cataria ◽

Natural Substances

Amini S, Nohooji MG, Khani M, Labbafi MR, Khalighi-Sigaroodi f. 2019. Biological activity of some essential oil constituents in four Nepeta L. species against Sitophilus oryzae L.. Biodiversitas 20: 338-343. Recently, there has been a growing interest in research concerning the possible use of plant extracts as alternatives to synthetic insecticides. Essential oils are among the best-known natural substances with insecticidal properties. These compounds may act as fumigants, contact insecticides, repellents, antifeedants and may affect growth and development of the insects. In an attempt to find a natural and inexpensive method for the control of stored-product pests, contact toxicity of essential oils of Nepeta cataria, Nepeta pogonosperma, Nepeta glomerulosa. and Nepeta binaloudensis. were investigated on adult insects of Sitophilus oryzae L. (Coleoptera, Curculionidae) in vitro condition. Chemical Composition, repellency and fumigant toxicity of the essential oils were investigated. Chemical composition of the essential oils of above plants were identified by GC-MS. 4aα,7α,7aβ-nepetalactone (82.74 %) was major component of N. cataria and also different isomers of nepetalactone were among the major compounds in N. Pogonosperma and N. binaloudensis oils. In fumigants bioassay, N. glomerulosa (124.318μL/L air) showed the highest toxicity against S. oryzae adults, followed by N.pogonosperma (150.49μL/L air) and N. cataria (152.630μL/L air), respectively. Also, the S. oryzae was repelled by N. cataria (91.61%) and N. binaloudensis (91.50 %), respectively.

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Epilobium angustifolium L. Essential Oil—Biological Activity and Enhancement of the Skin Penetration of Drugs—In Vitro Study

Molecules ◽

10.3390/molecules26237188 ◽

2021 ◽

Vol 26 (23) ◽

pp. 7188

Author(s):

Anna Nowak ◽

Wiktoria Duchnik ◽

Edyta Makuch ◽

Łukasz Kucharski ◽

Paula Ossowicz-Rupniewska ◽

...

Keyword(s):

Biological Activity ◽

Essential Oil ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Skin Penetration ◽

Percutaneous Penetration ◽

Potential Candidate ◽

High Biological Activity ◽

Epilobium Angustifolium

Epilobium angustifolium L. is a popular medicinal plant found in many regions of the world. This plant contains small amounts of essential oil whose composition and properties have not been extensively investigated. There are few reports in the literature on the antioxidant and antifungal properties of this essential oil and the possibility of applying it as a potential promoter of the skin penetration of drugs. The essential oil was obtained by distillation using a Clavenger type apparatus. The chemical composition was analyzed by the GC-MS method. The major active compounds of E. angustifolium L. essential oil (EOEa) were terpenes, including α-caryophyllene oxide, eucalyptol, β-linalool, camphor, (S)-carvone, and β-caryophyllene. The analyzed essential oil was also characterized by antioxidant activity amounting to 78% RSA (Radical Scavenging Activity). Antifungal activity against the strains Aspergillus niger, A. ochraceus, A. parasiticum, and Penicillium cyclopium was also determined. The largest inhibition zone was observed for strains from the Aspergillus group. The EOEa enhanced the percutaneous penetration of ibuprofen and lidocaine. After a 24 h test, the content of terpene in the skin and the acceptor fluid was examined. It has been shown that the main compounds contained in the essential oil do not penetrate through the skin, but accumulate in it. Additionally, FTIR-ATR analysis showed a disturbance of the stratum corneum (SC) lipids caused by the essential oil application. Due to its rich composition and high biological activity, EOEa may be a potential candidate to be applied, for example, in the pharmaceutical or cosmetic industries. Moreover, due to the reaction of the essential oil components with SC lipids, the EOEa could be an effective permeation enhancer of topically applied hydrophilic and lipophilic drugs.

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Helichrysum italicum (Roth) G. Don Essential Oil from Serbia: Chemical Composition, Classification and Biological Activity—May It Be a Suitable New Crop for Serbia?

Agronomy ◽

10.3390/agronomy11071282 ◽

2021 ◽

Vol 11 (7) ◽

pp. 1282

Author(s):

Milica Aćimović ◽

Jovana Ljujić ◽

Jelena Vulić ◽

Valtcho D. Zheljazkov ◽

Lato Pezo ◽

...

Keyword(s):

Biological Activity ◽

Chemical Composition ◽

Essential Oil ◽

Reducing Power ◽

Value Added ◽

Pharmaceutical Products ◽

Retention Indices ◽

Gas Chromatography Mass Spectrometry ◽

Bleaching Test

H. italicum essential oil (EO) is one of the most popular ingredients utilized by the cosmetic industry, and it is also used as natural antioxidant and as a value-added ingredient in food products. The chemical composition of the EO H. italicum cultivated in Serbia was analyzed by gas chromatography–mass spectrometry. The quantitative structure–retention relationship was used to predict the retention indices of the EO constituents acquired by GC-MS data, applying five molecular descriptors selected by factor analysis and a genetic algorithm. Also, antimicrobial activity, and biological activity by four common antioxidant tests (DPPH and ABTS assays, reducing power, and β-carotene bleaching test), and in vitro antihyperglycemic and anti-inflammatory capacities were evaluated. A total of 70 EO constituents were detected, of which 17 (8.5%) could not be identified. The H. italicum EO in this study belonged to γ-curcumene chemotype. The coefficients of determination reached the value of 0.964, demonstrating that this model could be used for prediction purposes. All applied tests showed that H. italicum EO possesses good biological activity and an interesting chemical composition. Therefore, the EO of H. italicum grown in Serbia has a potential to be used in food, cosmetic, and pharmaceutical products.

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Enhanced Biological Activity of a Novel Preparation of Lavandula angustifolia Essential Oil

Molecules ◽

10.3390/molecules26092458 ◽

2021 ◽

Vol 26 (9) ◽

pp. 2458

Author(s):

Małgorzata Miastkowska ◽

Tomasz Kantyka ◽

Ewa Bielecka ◽

Urszula Kałucka ◽

Marta Kamińska ◽

...

Keyword(s):

Biological Activity ◽

Essential Oil ◽

Wide Spectrum ◽

Skin Inflammation ◽

Angiogenic Factor ◽

Lavandula Angustifolia ◽

Lavender Oil ◽

Keratinocyte Cell ◽

Growing Conditions

Lavandula angustifolia, one of the most popular medicinal plants, is the source of a bioactive essential oil characterized by a wide spectrum of biological activity, e.g., antiseptic, analgesic, and anticancer effects. In dermatology, the oil helps to relieve skin inflammation and exhibit wound healing potential. However, the mechanism of action of the lavender oil depends on its composition, which in turn is dependent on the origin and growing conditions. Our study aimed to compare the composition and proregenerative properties of the commercially-available narrow-leaved lavender oil produced in Provence, France, with the oil obtained from the narrow-leaved lavender cultivated locally in Poland. GC/MS analysis showed that self-manufactured essential oil had lower linalool content than commercial oil (23.2 vs. 40.2%), comparable linalyl acetate content (40.6 vs. 44%), while the proportion of lavandulyl acetate was significantly higher (23.2 vs. 5.5%). To determine the influence of lavender oil on the production of proinflammatory cytokines and proregenerative growth factors, gene expression of the selected signaling molecules by HaCaT cells was investigated using real-time PCR. Results showed a concentration-dependent effect of lavender oils on the production of IL-6, IL-8, and VEGF by the keratinocyte cell line. Finally, the potential of the lavender oil to increase the production of VEGF, the most important angiogenic factor, with the in-house preparation performing significantly better in the in vitro cell models was identified.

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