Tomato stress-responsive factor TSRF1 interacts with ethylene responsive element GCC box and regulates pathogen resistance to Ralstonia solanacearum

E3 ubiquitin ligases, the most important part of the ubiquitination process, participate in various processes of plant immune response. RBR E3 ligase is one of the E3 family members, but its functions in plant immunity are still little known. NtRNF217 is a RBR E3 ligase in tobacco based on the sequence analysis. To assess roles of NtRNF217 in tobacco responding to Ralstonia solanacearum, overexpression experiments in Nicotiana tabacum (Yunyan 87, a susceptible cultivar) were performed. The results illuminated that NtRNF217-overexpressed tobacco significantly reduced multiplication of R. solanacearum and inhibited the development of disease symptoms compared with wild-type plants. The accumulation of H2O2 and O2− in NtRNF217-OE plants was significantly higher than that in WT-Yunyan87 plants after pathogen inoculation. The activities of CAT and SOD also increased rapidly in a short time after R. solanacearum inoculation in NtRNF217-OE plants. What is more, overexpression of NtRNF217 enhanced the transcript levels of defense-related marker genes, such as NtEFE26, NtACC Oxidase, NtHIN1, NtHSR201, and NtSOD1 in NtRNF217-OE plants after R. solanacearum inoculation. The results suggested that NtRNF217 played an important role in regulating the expression of defense-related genes and the antioxidant enzymes, which resulted in resistance to R. solanacearum infection.

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An AC-Rich Bean Element Serves as an Ethylene-Responsive Element in Arabidopsis

Plants ◽

10.3390/plants9081033 ◽

2020 ◽

Vol 9 (8) ◽

pp. 1033

Author(s):

Chunying Wang ◽

Tingting Lin ◽

Mengqi Wang ◽

Xiaoting Qi

Keyword(s):

Nuclear Proteins ◽

Ethylene Response Factor ◽

Response Factor ◽

Cis Acting ◽

Gcc Box ◽

Ethylene Response ◽

Significant Difference ◽

Responsive Element ◽

Transcription Factor 1

Ethylene-responsive elements (EREs), such as the GCC box, are critical for ethylene-regulated transcription in plants. Our previous work identified a 19-bp AC-rich element (ACE) in the promoter of bean (Phaseolus vulgaris) metal response element-binding transcription factor 1 (PvMTF-1). Ethylene response factor 15 (PvERF15) directly binds ACE to enhance PvMTF-1 expression. As a novel ERF-binding element, ACE exhibits a significant difference from the GCC box. Here, we demonstrated that ACE serves as an ERE in Arabidopsis. It conferred the minimal promoter to respond to the ethylene stress and inhibition of ethylene. Moreover, the cis-acting element ACE could specifically bind the nuclear proteins in vitro. We further revealed that the first 9-bp sequence of ACE (ACEcore) is importantly required by the binding of nuclear proteins. In addition, PvERF15 and PvMTF-1 were strongly induced by ethylene in bean seedlings. Since PvERF15 activates PvMTF-1 via ACE, ACE is involved in ethylene-induced PvMTF-1 expression. Taken together, our findings provide genetic and biochemical evidence for a new ERE.

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A complex network of additive and epistatic quantitative trait loci underlies natural variation of Arabidopsis thaliana quantitative disease resistance to Ralstonia solanacearum under heat stress

Molecular Plant Pathology ◽

10.1111/mpp.12964 ◽

2020 ◽

Vol 21 (11) ◽

pp. 1405-1420

Author(s):

Nathalie Aoun ◽

Henri Desaint ◽

Léa Boyrie ◽

Maxime Bonhomme ◽

Laurent Deslandes ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Heat Stress ◽

Disease Resistance ◽

Quantitative Trait Loci ◽

Complex Network ◽

Ralstonia Solanacearum ◽

Quantitative Trait ◽

Natural Variation ◽

Quantitative Disease Resistance ◽

Resistance To Ralstonia Solanacearum

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Overexpression of the peanut CLAVATA1-like leucine-rich repeat receptor-like kinase AhRLK1 confers increased resistance to bacterial wilt in tobacco

Journal of Experimental Botany ◽

10.1093/jxb/erz274 ◽

2019 ◽

Vol 70 (19) ◽

pp. 5407-5421

Author(s):

Chong Zhang ◽

Hua Chen ◽

Rui-Rong Zhuang ◽

Yu-Ting Chen ◽

Ye Deng ◽

...

Keyword(s):

Bacterial Wilt ◽

Ralstonia Solanacearum ◽

Defense Response ◽

R Gene ◽

Leucine Rich Repeat ◽

Receptor Like Kinase ◽

Defense Response Signaling ◽

Resistance To Ralstonia Solanacearum

Overexpression of a novel peanut CLAVATA1-like gene significantly enhanced the resistance to Ralstonia solanacearum in tobacco via defense response signaling associated with EDS1-mediated R-gene pathways.

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