Minor nutrients are critical for the improved growth of Corylus avellana shoot cultures

2014 ◽  
Vol 119 (2) ◽  
pp. 427-439 ◽  
Author(s):  
Charles Hand ◽  
Barbara M. Reed
2019 ◽  
pp. 105-115
Author(s):  
А.Ш. Хужахметова

Показана актуальность изучения экологической пластичности древесных растений в связи с необходимостью подбора адаптированного видового состава деревьев и кустарников для защитных лесных насаждений в условиях климатических изменений. Установлено, что экологическая пластичность и пределы толерантности растений связаны со свойством организмов адаптироваться к тому или иному диапазону факторов среды. В статье представлен таксономический состав орехоплодных культур коллекций ФНЦ агроэкологии РАН. Это шесть видов рода Juglans: J. regia, J. mandshurica, J. cinerea, J. rupestris, J. ailanthifolia, J. nigra и три вида рода Corylus: С. avellana L., С. аmericana W., сорта С. pontica C. Koch Президент, Футкурами, Черкесский2. Приведены сведения о положительном опыте культивирования Corylus avellana L. в плантационных насаждениях (1,6 га, посадка рядовая, схема размещения 55 м) в условиях южных черноземов. Цель исследований изучить экологическую пластичность орехоплодных кустарников коллекций ФНЦ агроэкологии РАН. На примере родового комплекса Corylus получены материалы по экологической пластичности орехоплодных кустарников в условиях каштановых (кадастр. 34:36:0000:14:0178), светлокаштановых почв (34:34:000000:122 34:34:060061:10). Установлены уровни изменчивости морфологических признаков ассимиляционного аппарата и плодов С. avellana L., сортов С. pontica C. Koch., которые согласуются с зимо и засухоустойчивостью. Кластерный анализ позволил выявить корреляцию признаков при 5 уровне значимости. Для целей защитного лесоразведения и озеленения засушливых районов рекомендованы Corylus avellana и Черкесский2 с выраженной вариабельностью морфологических признаков, которая указывает на их широкую экологическую валентность и адаптационные возможности в рассматриваемых условиях. The urgency of studying the ecological plasticity of woody plants in connection with the need to select an adapted species composition of trees and shrubs for protective forest plantations in the context of climate change is shown. It is established that the ecological plasticity and tolerance range of plants is associated with the ability of organisms to adapt to a particular range of environmental factors. The article presents the taxonomic composition of nut crops in the collections of FSC Agroecology RAS. Six species of Juglans: J. regia, J. mandshurica, J. cinerea, J. rupestris, J. ailanthifolia, J. nigra and three species of the genus Corylus: С. avellana L., С. аmericana W., varieties of C. pontica C. Koch the President, Futkurami, Circassian2. Data on positive experience of cultivation of Corylus avellana L. in plantation plantings (1,6 hectares, landing ordinary, the scheme of placement of 55 m) in the conditions of southern chernozems are given. The purpose of the research is to study the ecological plasticity of nutbearing shrubs of the collections of the Federal scientific center for Agroecology Russian Academy of Sciences. For example, a generic complex Corylus submissions received on the environmental plasticity of nut bushes in the conditions of brown (the cadaster nubmer 34:36:0000:14:0178), light chestnut soils (34:34:000000:122 34:34:060061:10). The levels of variability of morphological features of the assimilation apparatus and fruits of Corylus avellana L., С. pontica C. Koch varieties were established., which are consistent with winter and drought resistance. Cluster analysis revealed the correlation of features at 5 significance level. Corylus avellana and Circassian2 with a pronounced variability of morphological features, which indicates their broad ecological valence and adaptation capabilities in the conditions under consideration, are recommended for the purposes of protective afforestation and greening of dry areas.


HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 506d-506
Author(s):  
Robert R. Tripepi ◽  
Holly J. Schwager ◽  
Mary W. George ◽  
Joseph P. McCaffrey

Two insecticides, acephate or azadirachtin, were added to tissue culture media to determine their effectiveness in controlling onion thrips (Thrips tabaci Lindeman.) and to determine if these insecticides could damage the plant shoot cultures. To test for insecticide phytotoxicity, microshoots from European birch (Betula pendula), American elm (Ulmus americana), `Pink Arola' chrysanthemum (Dendranthema grandiflora), `America' rhododendron (Rhododendron catawbiense), `Golden Emblem' rose (Rosa hybrida), and `Gala' apple (Malus domestica) were placed in 130-ml baby food jars containing 25 ml of medium supplemented with 6.5, 13, or 26 mg/l Orthene® (contained acephate) or 0.55, 1.1, or 2.2 ml/l Azatin® (contained azadirachtin). Control jars lacked insecticide. To test for thrips control, 13 mg/l Orthene® or 0.55 ml/l Azatin® was added to Murashige and Skoog medium, and 10 thrips were placed on `Gala' apple microshoots in each jar. Jars were sealed with plastic wrap. In both studies, microshoot dry weight and heights were determined. In the second study, the total number of thrips per jar was also determined 3 weeks after inoculation. Microshoots on Orthene®-treated media lacked phytotoxicity symptoms, regardless of the concentration used. In contrast, Azatin® hindered plant growth, decreasing shoot height or dry weight by up to 85% depending on the species. Both insecticides prevented thrips populations from increasing, since less than 10 thrips were found in jars with insecticide-treated medium. Control jars, however, contained an average of almost 70 thrips per jar. This study demonstrated that both Orthene® and Azatin® were effective for eradicating thrips from plant tissue cultures, but Orthene® should probably be used because Azatin® was phytotoxic to all species tested.


1985 ◽  
Vol 70 (6) ◽  
pp. 687-692 ◽  
Author(s):  
M. M. Thompson ◽  
D. C. Smith ◽  
J. E. Burgess

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Mina Salehi ◽  
Siamak Farhadi ◽  
Ahmad Moieni ◽  
Naser Safaie ◽  
Mohsen Hesami

Abstract Background Paclitaxel is a well-known chemotherapeutic agent widely applied as a therapy for various types of cancers. In vitro culture of Corylus avellana has been named as a promising and low-cost strategy for paclitaxel production. Fungal elicitors have been reported as an impressive strategy for improving paclitaxel biosynthesis in cell suspension culture (CSC) of C. avellana. The objectives of this research were to forecast and optimize growth and paclitaxel biosynthesis based on four input variables including cell extract (CE) and culture filtrate (CF) concentration levels, elicitor adding day and CSC harvesting time in C. avellana cell culture, as a case study, using general regression neural network-fruit fly optimization algorithm (GRNN-FOA) via data mining approach for the first time. Results GRNN-FOA models (0.88–0.97) showed the superior prediction performances as compared to regression models (0.57–0.86). Comparative analysis of multilayer perceptron-genetic algorithm (MLP-GA) and GRNN-FOA showed very slight difference between two models for dry weight (DW), intracellular and extracellular paclitaxel in testing subset, the unseen data. However, MLP-GA was slightly more accurate as compared to GRNN-FOA for total paclitaxel and extracellular paclitaxel portion in testing subset. The slight difference was observed in maximum growth and paclitaxel biosynthesis optimized by FOA and GA. The optimization analysis using FOA on developed GRNN-FOA models showed that optimal CE [4.29% (v/v)] and CF [5.38% (v/v)] concentration levels, elicitor adding day (17) and harvesting time (88 h and 19 min) can lead to highest paclitaxel biosynthesis (372.89 µg l−1). Conclusions Great accordance between the predicted and observed values of DW, intracellular, extracellular and total yield of paclitaxel, and also extracellular paclitaxel portion support excellent performance of developed GRNN-FOA models. Overall, GRNN-FOA as new mathematical tool may pave the way for forecasting and optimizing secondary metabolite production in plant in vitro culture.


2021 ◽  
Vol 105 (3) ◽  
pp. 1215-1226
Author(s):  
Wojciech Makowski ◽  
Aleksandra Królicka ◽  
Anna Nowicka ◽  
Jana Zwyrtková ◽  
Barbara Tokarz ◽  
...  

Abstract The Venus flytrap (Dionaea muscipula J. Ellis) is a carnivorous plant able to synthesize large amounts of phenolic compounds, such as phenylpropanoids, flavonoids, phenolic acids, and 1,4-naphtoquinones. In this study, the first genetic transformation of D. muscipula tissues is presented. Two wild-type Rhizobium rhizogenes strains (LBA 9402 and ATCC 15834) were suitable vector organisms in the transformation process. Transformation led to the formation of teratoma (transformed shoot) cultures with the bacterial rolB gene incorporated into the plant genome in a single copy. Using high-pressure liquid chromatography, we demonstrated that transgenic plants were characterized by an increased quantity of phenolic compounds, including 1,4-naphtoquinone derivative, plumbagin (up to 106.63 mg × g−1 DW), and phenolic acids (including salicylic, caffeic, and ellagic acid), in comparison to non-transformed plants. Moreover, Rhizobium-mediated transformation highly increased the bactericidal properties of teratoma-derived extracts. The antibacterial properties of transformed plants were increased up to 33% against Staphylococcus aureus, Enterococcus faecalis, and Escherichia coli and up to 7% against Pseudomonas aeruginosa. For the first time, we prove the possibility of D. muscipula transformation. Moreover, we propose that transformation may be a valuable tool for enhancing secondary metabolite production in D. muscipula tissue and to increase bactericidal properties against human antibiotic-resistant bacteria. Key points • Rhizobium-mediated transformation created Dionaea muscipula teratomas. • Transformed plants had highly increased synthesis of phenolic compounds. • The MBC value was connected with plumbagin and phenolic acid concentrations.


Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 670
Author(s):  
Katalin Magyar-Tábori ◽  
Nóra Mendler-Drienyovszki ◽  
Alexandra Hanász ◽  
László Zsombik ◽  
Judit Dobránszki

In general, in vitro virus elimination is based on the culture of isolated meristem, and in addition thermotherapy, chemotherapy, electrotherapy, and cryotherapy can also be applied. During these processes, plantlets suffer several stresses, which can result in low rate of survival, inhibited growth, incomplete development, or abnormal morphology. Even though the in vitro cultures survive the treatment, further development can be inhibited; thus, regeneration capacity of treated in vitro shoots or explants play also an important role in successful virus elimination. Sensitivity of genotypes to treatments is very different, and the rate of destruction largely depends on the physiological condition of plants as well. Exposure time of treatments affects the rate of damage in almost every therapy. Other factors such as temperature, illumination (thermotherapy), type and concentration of applied chemicals (chemo- and cryotherapy), and electric current intensity (electrotherapy) also may have a great impact on the rate of damage. However, there are several ways to decrease the harmful effect of treatments. This review summarizes the harmful effects of virus elimination treatments applied on tissue cultures reported in the literature. The aim of this review is to expound the solutions that can be used to mitigate phytotoxic and other adverse effects in practice.


1965 ◽  
Vol 48 (6) ◽  
pp. 1100-1103
Author(s):  
C H Mcbride

Abstract The atomic absorption method studied last year was re-examined and extended to include calcium and sodium. The procedures were submitted to 16 collaborators for determination of Ca, Cu, Fe, Mg, Mn, Na, and Zn. Results for Ca and Na were discouraging; further study is recommended.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marziyeh Khavari ◽  
Reza Fatahi ◽  
Zabihollah Zamani

AbstractClimate change and population increase are two challenges for crop production in the world. Hazelnut (Corylus avellana L.) is considered an important nut regarding its nutritional and economic values. As a fact, the application of supporting materials as foliage sprays on plants will decrease biotic and abiotic stresses. In this study, the effects of salicylic acid (0, 1 mM and 2.5 mM) and kaolin (0, 3% and 6%) sprays were investigated on morphological, physiological, pomological, and biochemical characteristics of hazelnut. The results showed that 1 mM salicylic acid and 6% kaolin had the best effects on nut and kernel weight compared to control. Biochemical parameters such as chlorophyll a, b, a + b, and carotenoid contents showed that salicylic acid and kaolin improved pigment concentration. Proline and antioxidant contents such as phenolic acids, SOD, APX, and CAT enzyme activities increased by these applications. On the other hand, lipid peroxidation, protein content, and H2O2 content were decreased. Based on the tolerance index result, Merveille de Bollwiller cultivar showed the highest tolerance while 'Fertile de Coutard' had the lowest value. Therefore, hazelnut performance may be improved through exogenous application of the signaling (salicylic acid) and particle film (Kaolin) compounds in warmer climates.


Sign in / Sign up

Export Citation Format

Share Document