Ectopic expression of cucumber (Cucumis sativus L.) CsTIR/AFB genes enhance salt tolerance in transgenic Arabidopsis

2017 ◽  
Vol 131 (1) ◽  
pp. 107-118 ◽  
Author(s):  
Zhehao Chen ◽  
Mengting Li ◽  
Ye Yuan ◽  
Jiangqin Hu ◽  
Yanjun Yang ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Cucumis Sativus ◽  
Transgenic Arabidopsis ◽  
Ectopic Expression ◽  
Cucumis Sativus L

Overexpression of an APETALA1-like gene from cucumber (Cucumis sativus L.) induces earlier flowering and abnormal leaf development in transgenic Arabidopsis

2019 ◽  
Vol 99 (2) ◽  
pp. 210-220 ◽  
Author(s):  
Yong Zhou ◽  
Lifang Hu ◽  
Shuifeng Ye ◽  
Lunwei Jiang ◽  
Shiqiang Liu
Keyword(s):  
Cucumis Sativus ◽  
Plant Development ◽  
Flower Development ◽  
Leaf Development ◽  
Molecular Mechanisms ◽  
Transgenic Arabidopsis ◽  
Functional Characterization ◽  
Ectopic Expression ◽  
Mads Box ◽  
Cucumis Sativus L

MADS-box proteins are important transcription factors that play essential roles in various aspects of plant development, particularly in flower development. In this study, we performed the identification and functional characterization of CsMADS09 isolated from cucumber (Cucumis sativus L.). CsMADS09 contains a 648-bp open reading frame encoding 215 amino acid residues, and shares high sequence identities with the members of the AP1/FUL family of MADS-box proteins, especially the euAPETALA1 (euAP1) subclade. Many cis-elements related to plant development, stress response, and hormones were identified in the promoter region of CsMADS09. Quantitative real-time polymerase chain reaction results showed that CsMADS09 was mainly expressed in reproductive tissues such as male flowers and unexpanded ovaries, while its expression was low in roots and only traceable in fertilized ovaries. Moreover, the results revealed that CsMADS09 expression tended to decline during male flower development and stayed nearly constant during female flower development. Ectopic expression of CsMADS09 resulted in earlier flowering and abnormal leaf development in transgenic Arabidopsis. This study is the first functional analysis of an AP1-like gene from cucumber and provides some clues for revealing the molecular mechanisms of flower development in cucumber.

Potassium fluxes and reactive oxygen species production as potential indicators of salt tolerance in Cucumis sativus

2016 ◽  
Vol 43 (11) ◽  
pp. 1016 ◽  
Author(s):  
Mirvat Redwan ◽  
Francesco Spinelli ◽  
Lucia Marti ◽  
Matthias Weiland ◽  
Emily Palm ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Cucumis Sativus ◽  
Ros Production ◽  
Cucumis Sativus L ◽  
Ethylene Responsive Factor ◽  
Inward Rectifying Potassium Channel ◽  
Species Production ◽  
Respiratory Burst Oxidase ◽  
Respiratory Burst Oxidase Homologue

Salt stress, among other abiotic stresses, has a high impact on crop yield. Salt tolerance is a multifactorial trait that involves the ability of cells to retain K ions, regulate reactive O species (ROS) production, and synthesise new molecules to cope with osmotic stress. In the present work, two different cultivars of Cucumis sativus L. (cv. Parys, sensitive; cv. Polan, tolerant) were selected based on their germination capabilities under 100 mM NaCl. The capacity of these two cultivars to tolerate salt stress was analysed using several different physiological and genetic approaches. K+ fluxes from roots, as an immediate response to salinity, showed the higher ability of cv. Polan to maintain K+ compared with cv. Parys, according to the expression level of inward rectifying potassium channel 1 (AKT1). ROS production was also investigated in both cultivars and a higher basal ROS level was observed in cv. Polan than in cv. Parys. Concurrently, an increased basal level of respiratory burst oxidase homologue F (RBOHF) gene was also found, as well as a strong induction of the ethylene responsive factor 109 (ERF109) transcription factor after salt treatment in cv. Polan. Our data suggest that roots’ ability to retain K+, a higher level of RBOHF and a strong induction of ERF109 should all be considered important components for salt tolerance in C. sativus.

Salicylic acid and kinetin mediated stimulation of salt tolerance in cucumber (Cucumis sativus L.) genotypes varying in salinity tolerance

2018 ◽  
Vol 59 (4) ◽  
pp. 461-471 ◽  
Author(s):  
Ali Raza Gurmani ◽  
Sami Ullah Khan ◽  
Amjad Ali ◽  
Tehseen Rubab ◽  
Timothy Schwinghamer ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Salt Tolerance ◽  
Cucumis Sativus ◽  
Salinity Tolerance ◽  
Cucumis Sativus L ◽  
Stimulation Of

scholarly journals Ectopic Expression of Gs5PTase8, a Soybean Inositol Polyphosphate 5-Phosphatase, Enhances Salt Tolerance in Plants

2020 ◽  
Vol 21 (3) ◽  
pp. 1023 ◽  
Author(s):  
Qi Jia ◽  
Song Sun ◽  
Defeng Kong ◽  
Junliang Song ◽  
Lumei Wu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Stress Responses ◽  
Transgenic Arabidopsis ◽  
Glycine Soja ◽  
Critical Role ◽  
Ectopic Expression ◽  
Wild Soybean ◽  
Inositol Polyphosphate ◽  
Wild Type

Inositol polyphosphate 5-phosphatases (5PTases) function in inositol signaling by regulating the catabolism of phosphoinositol derivatives. Previous reports showed that 5PTases play a critical role in plant development and stress responses. In this study, we identified a novel 5PTase gene, Gs5PTase8, from the salt-tolerance locus of chromosome 3 in wild soybean (Glycine soja). Gs5PTase8 is highly up-regulated under salt treatment. It is localized in the nucleus and plasma membrane with a strong signal in the apoplast. Ectopic expression of Gs5PTase8 significantly increased salt tolerance in transgenic BY-2 cells, soybean hairy roots and Arabidopsis, suggesting Gs5PTase8 could increase salt tolerance in plants. The overexpression of Gs5PTase8 significantly enhanced the activities of catalase and ascorbate peroxidase under salt stress. The seeds of Gs5PTase8-transgenic Arabidopsis germinated earlier than the wild type under abscisic acid treatment, indicating Gs5PTase8 would alter ABA sensitivity. Besides, transcriptional analyses showed that the stress-responsive genes, AtRD22, AtRD29A and AtRD29B, were induced with a higher level in the Gs5PTase8-transgenic Arabidopsis plants than in the wild type under salt stress. These results reveal that Gs5PTase8 play a positive role in salt tolerance and might be a candidate gene for improving soybean adaptation to salt stress.

scholarly journals Genome-wide analysis of OVATE family proteins in cucumber (Cucumis sativus L.)

2019 ◽  
Author(s):  
Lijie Han ◽  
Xiaofei Song ◽  
Zhongyi Wang ◽  
Xiaofeng Liu ◽  
Liying Yan ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Expression Patterns ◽  
Ectopic Expression ◽  
Reproductive Organs ◽  
Cucumis Sativus L ◽  
Hormone Response Elements ◽  
Genome Wide ◽  
Cucumber Fruit ◽  
Specific Proteins ◽  
Gibberellin Treatment

Abstract Background: OVATE family proteins (OFPs) are plant-specific proteins with the conserved OVATE domain that regulating plant growth and development. Although these OFPs have been studied in several species, the biological functions of this OFP gene family remain largely unknown in cucumber (Cucumis sativus L.). Results: In this study, we identified 19 CsOFPs in cucumber. This CsOFPs are distributed on seven chromosomes and can be divided into four subgroups. Most CsOFP genes are expressed in reproductive organs although have different expression patterns. Cis-elements analysis showed that there are six kinds of hormone response elements in CsOFPs and exogenous gibberellin treatment leads to a ‘first increase then decrease’ expression pattern of CsOFP7, CsOFP11 and CsOFP12. Ectopic expression of CsOFP11 in Arabidopsis resulted in shorter and blunt siliques. Conclusions: Together, these results indicated that CsOFPs may play important roles in cucumber fruit development.

Silicon improves salt tolerance by increasing root water uptake in Cucumis sativus L.

2015 ◽  
Vol 34 (9) ◽  
pp. 1629-1646 ◽  
Author(s):  
Yong-Xing Zhu ◽  
Xuan-Bin Xu ◽  
Yan-Hong Hu ◽  
Wei-Hua Han ◽  
Jun-Liang Yin ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Cucumis Sativus ◽  
Water Uptake ◽  
Root Water Uptake ◽  
Cucumis Sativus L

scholarly journals Identification and Characterization of a SEPALLATA-like MADS-box Gene from Cucumber (Cucumis sativus L.)

2019 ◽  
Vol 47 (4) ◽  
pp. 1168-1177 ◽  
Author(s):  
Yanwei CHENG ◽  
Peng HE ◽  
Lunwei JIANG ◽  
Shiqiang LIU ◽  
Yong ZHOU
Keyword(s):  
Cucumis Sativus ◽  
Growth And Development ◽  
Ectopic Expression ◽  
Digital Object Identifier ◽  
Amino Acid Residues ◽  
Mads Box ◽  
Cucumis Sativus L ◽  
Mads Box Gene ◽  
Identification And Characterization

SEPALLATA (SEP) genes encode the E class MADS-box transcription factors that play vital roles in various aspects of plant growth and development. In this study, we isolated a SEP-like gene from cucumber (Cucumis sativus L.), which was previously named as CsMADS01. CsMADS01 had a coding sequence (CDS) of 741 bp, and coded a deduced protein of 246 amino acid residues that was predicted to be located in the nucleus. The putative CsMADS01 protein was typically characterized by the MIKC-type MADS (including MADS, I, K, and C domains) and shared high homology with other SEP-like proteins. Phylogenetic analysis of CsMADS01 and SEP proteins from other plants revealed that CsMADS01 was a member of the SEP1/2 clade of SEP proteins and was the most closely related to ZjMADS48 from Chinese jujube. Promoter analysis showed that several cis-elements related to stress response and hormones were present in the promoter region of CsMADS01. Expression analysis indicated that CsMADS01 was highly expressed during the development of male and female flowers, and the expression increased gradually along with fruit development. Ectopic expression of CsMADS01 in Arabidopsis resulted in a phyllody-like phenotype and the transgenic plants never flowered. These results suggest that CsMADS01 plays an important role in the growth and development of cucumber.   ********* In press - Online First. Article has been peer reviewed, accepted for publication and published online without pagination. It will receive pagination when the issue will be ready for publishing as a complete number (Volume 47, Issue 4, 2019). The article is searchable and citable by Digital Object Identifier (DOI). DOI link will become active after the article will be included in the complete issue. *********

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