Overexpression of the mango MiCO gene delayed flowering time in transgenic Arabidopsis

2020 ◽  
Vol 143 (1) ◽  
pp. 219-228
Author(s):  
Yuan Liu ◽  
Cong Luo ◽  
Xiu-Juan Zhang ◽  
Xin-Xi Lu ◽  
Hai-Xia Yu ◽  
...  
Keyword(s):  
Flowering Time ◽  
Transgenic Arabidopsis ◽  
Delayed Flowering

scholarly journals Expression of a NGATHA1 Gene from Medicago truncatula Delays Flowering Time and Enhances Stress Tolerance

2020 ◽  
Vol 21 (7) ◽  
pp. 2384
Author(s):  
Tao Guo ◽  
Shumin Wang ◽  
Yinruizhi Li ◽  
Jianbo Yuan ◽  
Lixin Xu ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Stress Tolerance ◽  
Flowering Time ◽  
Medicago Truncatula ◽  
Transgenic Arabidopsis ◽  
Hormone Synthesis ◽  
Cis Acting ◽  
Plant Expression Vector ◽  
Lateral Organ ◽  
Delayed Flowering

Shoot branching is one of the most variable determinants of crop yield, and the signaling pathways of plant branches have become a hot research topic. As an important transcription factor in the B3 family, NGATHA1 (NGA1), plays an important role in regulating plant lateral organ development and hormone synthesis and transport, but few studies of the role of this gene in the regulation of plant growth and stress tolerance have been reported. In this study, the NGA1 gene was isolated from Medicago truncatula (Mt) and its function was characterized. The cis-acting elements upstream of the 5′ end of MtNGA1 and the expression pattern of MtNGA1 were analyzed, and the results indicated that the gene may act as a regulator of stress resistance. A plant expression vector was constructed and transgenic Arabidopsis plants were obtained. Transgenic Arabidopsis showed delayed flowering time and reduced branching phenotypes. Genes involved in the regulation of branching and flowering were differentially expressed in transgenic plants compared with wild-type plants. Furthermore, transgenic plants demonstrated strong tolerances to salt- and mannitol-induced stresses, which may be due to the upregulated expression of NCED3 (NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3) by the MtNGA1 gene. These results provide useful information for the exploration and genetic modification use of MtNGA1 in the future.

scholarly journals Potential transceptor AtNRT1.13 modulates shoot architecture and flowering time in a nitrate-dependent manner

2021 ◽  
Author(s):  
Hui-Yu Chen ◽  
Shan-Hua Lin ◽  
Ling-Hsin Cheng ◽  
Jeng-Jong Wu ◽  
Yi-Chen Lin ◽  
...  
Keyword(s):  
Flowering Time ◽  
Nitrate Transport ◽  
Dependent Manner ◽  
Transport Activity ◽  
Node Number ◽  
Shoot Architecture ◽  
Glucuronidase Reporter ◽  
Uptake Activity ◽  
Delayed Flowering ◽  
Severe Growth

Abstract Compared with root development regulated by external nutrients, less is known about how internal nutrients are monitored to control plasticity of shoot development. In this study, we characterize an Arabidopsis thaliana transceptor, NRT1.13 (NPF4.4), of the NRT1/PTR/NPF family. Different from most NRT1 transporters, NRT1.13 does not have the conserved proline residue between transmembrane domains 10 and 11; an essential residue for nitrate transport activity in CHL1/NRT1.1/NPF6.3. As expected, when expressed in oocytes, NRT1.13 showed no nitrate transport activity. However, when Ser 487 at the corresponding position was converted back to proline, NRT1.13 S487P regained nitrate uptake activity, suggesting that wild-type NRT1.13 cannot transport nitrate but can bind it. Subcellular localization and β-glucuronidase reporter analyses indicated that NRT1.13 is a plasma membrane protein expressed at the parenchyma cells next to xylem in the petioles and the stem nodes. When plants were grown with a normal concentration of nitrate, nrt1.13 showed no severe growth phenotype. However, when grown under low-nitrate conditions, nrt1.13 showed delayed flowering, increased node number, retarded branch outgrowth, and reduced lateral nitrate allocation to nodes. Our results suggest that NRT1.13 is required for low-nitrate acclimation and that internal nitrate is monitored near the xylem by NRT1.13 to regulate shoot architecture and flowering time.

scholarly journals A Composite Analysis of Flowering Time Regulation in Lettuce

2021 ◽  
Vol 12 ◽  
Author(s):  
Rongkui Han ◽  
Maria José Truco ◽  
Dean O. Lavelle ◽  
Richard W. Michelmore
Keyword(s):  
Flowering Time ◽  
Crop Production ◽  
Phenotypic Diversity ◽  
Model Organism ◽  
Leafy Vegetables ◽  
Vegetable Crops ◽  
Comprehensive Overview ◽  
Molecular Studies ◽  
Functional Inference ◽  
Delayed Flowering

Plants undergo profound physiological changes when transitioning from vegetative to reproductive growth. These changes affect crop production, as in the case of leafy vegetables. Lettuce is one of the most valuable leafy vegetable crops in the world. Past genetic studies have identified multiple quantitative trait loci (QTLs) that affect the timing of the floral transition in lettuce. Extensive functional molecular studies in the model organism Arabidopsis provide the opportunity to transfer knowledge to lettuce to explore the mechanisms through which genetic variations translate into changes in flowering time. In this review, we integrated results from past genetic and molecular studies for flowering time in lettuce with orthology and functional inference from Arabidopsis. This summarizes the basis for all known genetic variation underlying the phenotypic diversity of flowering time in lettuce and how the genetics of flowering time in lettuce projects onto the established pathways controlling flowering time in plants. This comprehensive overview reveals patterns across experiments as well as areas in need of further study. Our review also represents a resource for developing cultivars with delayed flowering time.

scholarly journals Interaction Between Induced and Natural Variation at oil yellow1 Delays Reproductive Maturity in Maize

2019 ◽  
Vol 10 (2) ◽  
pp. 797-810
Author(s):  
Rajdeep S. Khangura ◽  
Bala P. Venkata ◽  
Sandeep R. Marla ◽  
Michael V. Mickelbart ◽  
Singha Dhungana ◽  
...  
Keyword(s):  
Flowering Time ◽  
Chlorophyll Content ◽  
Chlorophyll Biosynthesis ◽  
Wild Type ◽  
Reproductive Maturity ◽  
Magnesium Chelatase ◽  
Chlorophyll Contents ◽  
Delayed Flowering ◽  
Mapping Populations ◽  
Expression Polymorphism

We previously demonstrated that maize (Zea mays) locus very oil yellow1 (vey1) encodes a putative cis-regulatory expression polymorphism at the magnesium chelatase subunit I gene (aka oil yellow1) that strongly modifies the chlorophyll content of the semi-dominant Oy1-N1989 mutants. The vey1 allele of Mo17 inbred line reduces chlorophyll content in the mutants leading to reduced photosynthetic output. Oy1-N1989 mutants in B73 reached reproductive maturity four days later than wild-type siblings. Enhancement of Oy1-N1989 by the Mo17 allele at the vey1 QTL delayed maturity further, resulting in detection of a flowering time QTL in two bi-parental mapping populations crossed to Oy1-N1989. The near isogenic lines of B73 harboring the vey1 allele from Mo17 delayed flowering of Oy1-N1989 mutants by twelve days. Just as previously observed for chlorophyll content, vey1 had no effect on reproductive maturity in the absence of the Oy1-N1989 allele. Loss of chlorophyll biosynthesis in Oy1-N1989 mutants and enhancement by vey1 reduced CO2 assimilation. We attempted to separate the effects of photosynthesis on the induction of flowering from a possible impact of chlorophyll metabolites and retrograde signaling by manually reducing leaf area. Removal of leaves, independent of the Oy1-N1989 mutant, delayed flowering but surprisingly reduced chlorophyll contents of emerging leaves. Thus, defoliation did not completely separate the identity of the signal(s) that regulates flowering time from changes in chlorophyll content in the foliage. These findings illustrate the necessity to explore the linkage between metabolism and the mechanisms that connect it to flowering time regulation.

The effect of leaf removal on flowering time in subterranean clover

1970 ◽  
Vol 21 (6) ◽  
pp. 893 ◽  
Author(s):  
WJ Collins ◽  
Y Aitken
Keyword(s):  
Flowering Time ◽  
Subterranean Clover ◽  
Early Growth ◽  
Grazing Management ◽  
Flower Initiation ◽  
Leaf Removal ◽  
Main Shoot ◽  
Delayed Flowering ◽  
Leaf Appearance ◽  
Rate Of Leaf Appearance

The removal of fully expanded leaves delayed flowering by up to 30 days in subterranean clover cv. Mt. Barker sown in winter at Melbourne (38�S.). This effect on flowering was attributable partly to a delay in flower initiation and partly to a slower rate of leaf appearance after flower initiation. Thus leaf removal may be added to the factors already known to influence flower initiation in subterranean clover. When plants were grown under a 24 hr photoperiod. leaf removal had no effect on flower initiation; the slight delay that leaf removal caused in flowering was therefore due entirely to its effect in reducing the rate of leaf appearance. In other experiments leaf removal delayed the time of flower initiation but had no effect on the rate of leaf appearance. The effect of leaf removal on the time of flowering on the main shoot in lateral-dominant plants (as occur in the field) was qualitatively the same as in plants from which the laterals had been removed. Grazing management of subterranean clover which results in severe defoliation during early growth may delay flowering to such an extent that seed production is reduced substantially, and persistence thereby prejudiced.

scholarly journals Overexpression of PvGF14c from Phyllostachys violascens Delays Flowering Time in Transgenic Arabidopsis

2018 ◽  
Vol 9 ◽  
Author(s):  
Bingjuan Li ◽  
Guohui Xiao ◽  
Kaisheng Luo ◽  
Zhengyi Wang ◽  
Bizeng Mao ◽  
...  
Keyword(s):  
Flowering Time ◽  
Transgenic Arabidopsis

scholarly journals Spatiotemporal expression of FRIGIDA modulate flowering time in Arabidopsis thaliana

2018 ◽  
Author(s):  
Xiangxiang Kong ◽  
Jinjie Zhao ◽  
Landi Luo ◽  
Qian Chen ◽  
Guanxiao Chang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Flowering Time ◽  
Environmental Cues ◽  
The Novel ◽  
Spatiotemporal Expression ◽  
Embryo Stage ◽  
Novel Targets ◽  
Delayed Flowering ◽  
Fine Tune ◽  
Local Expression

AbstractFRIGIDA (FRI) as the major regulator of flowering time in Arabidopsis accessions can activate its target FLOWERING LOCUS C (FLC) to delay flowering before vernalization. Besides FLC, other FRI targets also exist in Arabidopsis. Although leaves sense environmental cues to modulate flowering time, it is not known if roots also regulate the floral transition. In this study, we investigated the spatiotemporal effect of FRI on flowering time. Local expression of FRI in the phloem and leaves activated FLC to delay flowering. Furthermore, we found that local expression of FRI in the roots also delayed flowering by activating other targets MADS AFFECTING FLOWERING4 (MAF4) and MAF5 in the roots. Graft and genetic experiments revealed that the spatial expression of FRI in the root might generate a mobile signal, which is transmitted from roots to shoot and antagonizes the FT signal to delay flowering. Specifically expressing FRI in the embryo efficiently delayed flowering, even expressing FRI as early as pro-embryo stage is enough to upregulate FLC expression to delay flowering. Together, our findings confirm the spatiotemporal effect of FRI on delaying flowering, and propose that root tissue also perceives the flowering signal to fine-tune the flowering time through MAF4/5 as novel targets of FRI.HighlightRoot FRIDIGA activated the novel targets MAF4/5 to delay flowering; Temporal expressing FRIGIDA at as early as pro-embryo stage is efficient to delay flowering.

scholarly journals Combined Analysis of MicroRNAs and Target Genes Revealed miR156-SPLs and miR172-AP2 Are Involved in a Delayed Flowering Phenomenon After Chromosome Doubling in Black Goji (Lycium ruthencium)

2021 ◽  
Vol 12 ◽  
Author(s):  
Shupei Rao ◽  
Yue Li ◽  
Jinhuan Chen
Keyword(s):  
Flowering Time ◽  
Messenger Rna ◽  
Target Genes ◽  
Higher Plants ◽  
Chromosome Doubling ◽  
Late Flowering ◽  
New Perspective ◽  
Commercial Applications ◽  
Flowering Regulation ◽  
Delayed Flowering

Polyploidy, which is widely distributed in angiosperms, presents extremely valuable commercial applications in plant growth and reproduction. The flower development process of higher plants is essential for genetic improvement. Nevertheless, the reproduction difference between polyploidy and the polyploid florescence regulatory network from the perspective of microRNA (miRNA) remains to be elucidated. In this study, the autotetraploid of Lycium ruthenicum showed late-flowering traits compared with the progenitor. Combining the association of miRNA and next-generation transcriptome technology, the late-flowering characteristics triggered by chromosome duplication may be caused by the age pathway involved in miR156-SPLs and miR172-AP2, which inhibits the messenger RNA (mRNA) transcripts of FT in the leaves. Subsequently, FT was transferred to the shoot apical meristem (SAM) to inhibit the expression of the flowering integration factor SOC1, which can eventually result in delayed flowering time. Our exploration of the flowering regulation network and the control of the flowering time are vital to the goji producing in the late frost area, which provides a new perspective for exploring the intrinsic molecular mechanism of polyploid and the reproductive development of flowering plants.

Sign in / Sign up

Export Citation Format

Share Document