scholarly journals Establishment of callus induction system, histological evaluation and taxifolin production of Larch

2021 ◽  
Author(s):  
Xinglong Liu ◽  
Yingchun Zhao ◽  
Xueyan Chen ◽  
Ling Dong ◽  
Yinan Zheng ◽  
...  
Keyword(s):  
Callus Induction ◽  
Histological Evaluation ◽  
Induction System

scholarly journals Establishment of callus induction system, histological evaluation and taxifolin production of Larch

2021 ◽  
Author(s):  
Xinglong Liu ◽  
Yingchun Zhao ◽  
Xueyan Chen ◽  
Ling Dong ◽  
Yinan Zheng ◽  
...  
Keyword(s):  
Callus Culture ◽  
Callus Induction ◽  
Callus Formation ◽  
Ecological Environment ◽  
Histological Evaluation ◽  
Larix Olgensis ◽  
Light Conditions ◽  
Induction Condition ◽  
Induction System ◽  
Optimal Medium

Abstract Taxifolin in larch is an important natural active ingredient, which prevents and treats liver diseases and cardiovascular diseases, and has many health benefits and application values ​​such as anti-tumor, anti-virus, and anti-aging. However, the traditional extraction method is not conducive to the protection of the ecological environment, and also limits the industrial production of taxifolin from larch. Therefore, the purpose of this research is to establish a larch callus culture system and obtain taxifolin from callus. The shoots of the stem-tips of Larix olgensis were used as explants to be cultured. The light conditions, optimal medium and hormone ratio for callus induction were screened to evaluate the callus induction characteristics of larch and its influence on the accumulation of taxifolin. The results showed that: callus formation started on 28–51 days, and the best conditions for inducing callus were explored: 12 h/d light culture MS + 2,4-D 2 mg/L + 6-BA 1 mg/ L + KT 0.1 mg/L; the callus induction rate is 50%-80%; the highest condition for producing taxifolin 6-BA 1 mg/L + NAA 0.1 mg/L, reaching 0.414%. In conclusion, this study represents a suitable induction condition for larch callus culture and taxifolin accumulation.

scholarly journals Establishment and optimization of high efficiency embryogenic callus induction system in Dendrobium candidum

2016 ◽  
Vol 10 (4) ◽  
pp. 77-83
Author(s):  
Mohammed Reda Refish Nada ◽  
Wang Linqing ◽  
Fu Chunhua ◽  
Xu Xiangping ◽  
Jin Wenwen ◽  
...  
Keyword(s):  
Callus Induction ◽  
Embryogenic Callus ◽  
High Efficiency ◽  
Induction System ◽  
Dendrobium Candidum ◽  
Embryogenic Callus Induction

scholarly journals The Establishment of an Efficient Callus Induction System for Lotus (Nelumbo nucifera)

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1436
Author(s):  
Xianbao Deng ◽  
Yaqian Xiong ◽  
Jing Li ◽  
Dong Yang ◽  
Juan Liu ◽  
...  
Keyword(s):  
Callus Induction ◽  
Developmental Stages ◽  
Callus Formation ◽  
Immature Embryo ◽  
Basal Medium ◽  
Nelumbo Nucifera ◽  
Dichlorophenoxyacetic Acid ◽  
Highly Efficient ◽  
Induction System ◽  
2,4 Dichlorophenoxyacetic Acid

The lotus (Nelumbo nucifera) is one of the most popular aquatic plants in Asia, and has emerged as a novel model for studying flower and rhizome development, and primary and secondary metabolite accumulation. Here, we developed a highly efficient callus induction system for the lotus by optimizing a series of key factors that affect callus formation. The highest efficient callus production was induced on immature cotyledon and embryo explants grown on Murashige and Skoog (MS) basal medium containing an optimized combination of 3 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/L 6-benzylaminopurine (6-BA). In addition, lotus callus induction was proven to be influenced by lotus genotypes, light conditions, the developmental stages of explants and the time of explant sampling. Collecting immature cotyledons from seeds of the genotype “Shilihe 1”, at 9 days post pollination, and to culture the explants in darkness, are proposed as the optimum conditions for lotus callus induction. Interestingly, highly efficient callus induction was also observed in explants of immature embryo derived aseptic seedlings; and a small amount of lotus benzylisoquinoline alkaloid (BIA) and obvious expression of BIA biosynthetic genes were detected in lotus callus.

Histological evaluation of cochlear hair cell damage from noise-induced hearing loss in chinchillas

1990 ◽  
Vol 48 (3) ◽  
pp. 332-333
Author(s):  
R.V. Harrison ◽  
R.J. Mount ◽  
P. White ◽  
N. Fukushima
Keyword(s):  
Hair Cell ◽  
Evoked Potential ◽  
Cell Damage ◽  
Acoustic Trauma ◽  
Histological Evaluation ◽  
Cell Integrity ◽  
Functional Changes ◽  
Cochlear Hair Cell ◽  
Left And Right ◽  
Contralateral Ear

In studies which attempt to define the influence of various factors on recovery of hair cell integrity after acoustic trauma, an experimental and a control ear which initially have equal degrees of damage are required. With in a group of animals receiving an identical level of acoustic trauma there is more symmetry between the ears of each individual, in respect to function, than between animals. Figure 1 illustrates this, left and right cochlear evoked potential (CAP) audiograms are shown for two chinchillas receiving identical trauma. For this reason the contralateral ear is used as control.To compliment such functional evaluations we have devised a scoring system, based on the condition of hair cell stereocilia as revealed by scanning electron microscopy, which permits total stereociliar damage to be expressed numerically. This quantification permits correlation of the degree of structural pathology with functional changes. In this paper wereport experiments to verify the symmetry of stereociliar integrity between two ears, both for normal (non-exposed) animals and chinchillas in which each ear has received identical noise trauma.

The Effect of Dietary Glutathione and Coenzyme Q10 on the Prevention and Treatment of Inflammatory Bowel Disease in Mice

2004 ◽  
Vol 74 (1) ◽  
pp. 74-85 ◽  
Author(s):  
Liu ◽  
Russell ◽  
Smith ◽  
Bronson ◽  
Milbury ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Dextran Sulfate ◽  
Coenzyme Q ◽  
Histological Evaluation ◽  
Therapeutic Effects ◽  
Prevention Study ◽  
Prevention And Treatment ◽  
Inflammatory Bowel ◽  
Pre Treatment

Because reactive oxygen species have been implicated as mediators of inflammatory bowel disease (IBD), we evaluated the potential preventive and therapeutic effects of two dietary antioxidants, glutathione (GSH) and coenzyme Q10 (CoQ10) on dextran sulfate sodium (DSS)-induced colitis in mice. Fifty female 8-wk old Swiss-Webster mice were randomly assigned to 4 groups for a pre-treatment 'prevention' study: (1) GSH (1% of diet); (2) CoQ10 (200 mg/kg/d); (3) DSS only (3% of drinking water); (4) control (no treatment). The mice in groups 1 and 2 were fed with GSH or CoQ10 for 21 wks, and the mice in groups 1, 2 and 3 were provided DSS from wk 7 for 4 cycles (1 cycle = 1 wk DSS followed by 2-wk water). Another 50 mice were randomly assigned to 4 groups for a 21-wk 'treatment' study where the mice in groups 1, 2, and 3 were administered DSS for 6 cycles (18 wks) to induce colitis. GSH and CoQ10 were added from wk 7 until the completion of the protocol. Loose stools and hemocult positivity were modestly but significantly reduced with GSH or CoQ10 at several periods during the intervention in both the prevention and treatment studies. In contrast, histological evaluation revealed increases in colonic dysplasia and ulceration with GSH or CoQ10. Thus, in this mouse model, GSH and CoQ10 appear to have a beneficial effect on acute signs of IBD, but may have an adverse impact on the chronic pathophysiology of the disease. Further studies using additional animal models are required to determine whether GSH or CoQ10 provide a favorable or unfavorable benefit:risk ratio in the prevention or treatment of IBD.

Adenine salvage activity during callus induction and plant growth

1994 ◽  
Vol 90 (4) ◽  
pp. 739-747 ◽  
Author(s):  
Diana Lee ◽  
Barbara A. Moffatt
Keyword(s):  
Plant Growth ◽  
Callus Induction

An unusual case of spinal cord restricted mycobacteriosis in a European mink

2013 ◽  
Vol 41 (01) ◽  
pp. 63-66
Author(s):  
D. Schaudien ◽  
C. Flieshardt ◽  
I. Moser ◽  
H. Hotzel ◽  
A. Tipold ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Unusual Case ◽  
Histological Evaluation ◽  
Mustela Lutreola ◽  
Chain Reaction ◽  
European Mink ◽  
Pcr Product ◽  
Granulomatous Lesions ◽  
The Central Nervous System ◽  
Polymerase Chain

SummaryGranulomatous myelitis due to infection with Mycobacterium avium was diagnosed in a 4-year-old male neutered European mink (Mustela lutreola). The causative agent was detected by an acid-fast stain and further characterized by polymerase chain reaction and DNA sequencing of the PCR product. A thorough histological evaluation of the remaining organs revealed no granulomatous lesions or detectable acid-fast organisms. Although minks are generally highly susceptible for mycobacteria, localised infections, especially of the central nervous system, are unusual and may represent an atypical chronic form of the disease.

Experimental and Numerical Analysis of a Motorcycle Air Intake System Aerodynamics and Performance

2020 ◽  
Vol 17 (1) ◽  
Author(s):  
M. A. Abd Halim ◽  
N. A. R. Nik Mohd ◽  
M. N. Mohd Nasir ◽  
M. N. Dahalan
Keyword(s):  
Combustion Process ◽  
Three Dimensional ◽  
Engine Performance ◽  
Internal Flow ◽  
Rapid Expansion ◽  
Navier Stokes ◽  
Turbulent Fluctuation ◽  
Ansys Fluent ◽  
Induction System ◽  
Acceleration And Deceleration

Induction system or also known as the breathing system is a sub-component of the internal combustion system that supplies clean air for the combustion process. A good design of the induction system would be able to supply the air with adequate pressure, temperature and density for the combustion process to optimizing the engine performance. The induction system has an internal flow problem with a geometry that has rapid expansion or diverging and converging sections that may lead to sudden acceleration and deceleration of flow, flow separation and cause excessive turbulent fluctuation in the system. The aerodynamic performance of these induction systems influences the pressure drop effect and thus the engine performance. Therefore, in this work, the aerodynamics of motorcycle induction systems is to be investigated for a range of Cubic Feet per Minute (CFM). A three-dimensional simulation of the flow inside a generic 4-stroke motorcycle airbox were done using Reynolds-Averaged Navier Stokes (RANS) Computational Fluid Dynamics (CFD) solver in ANSYS Fluent version 11. The simulation results are validated by an experimental study performed using a flow bench. The study shows that the difference of the validation is 1.54% in average at the total pressure outlet. A potential improvement to the system have been observed and can be done to suit motorsports applications.

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