Bioremediation of Heavy Metals by a Novel Bacterial Strain Enterobacter cloacae and Its Antioxidant Enzyme Activity, Flocculant Production, and Protein Expression in Presence of Lead, Cadmium, and Nickel

2015 ◽  
Vol 226 (4) ◽  
Author(s):  
Goutam Banerjee ◽  
Shubhant Pandey ◽  
Arun Kumar Ray ◽  
Ravi Kumar
Keyword(s):  
Heavy Metals ◽  
Enzyme Activity ◽  
Protein Expression ◽  
Antioxidant Enzyme ◽  
Bacterial Strain ◽  
Enterobacter Cloacae ◽  
Antioxidant Enzyme Activity

P4428Physical exercise during uncontrolled systemic arterial hypertension

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
L Urbano Pagan ◽  
M J Gomes ◽  
R L Damatto ◽  
M D M Cezar ◽  
D R A Reyes ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Protein Expression ◽  
Antioxidant Enzyme ◽  
Cardiac Remodeling ◽  
Spontaneously Hypertensive Rats ◽  
Myocardial Function ◽  
Antioxidant Enzyme Activity ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Nadph Oxidase Activity

Abstract Introduction Physical exercise reduces systemic arterial blood pressure and improves cardiac remodeling. However, the effects of exercise during uncontrolled arterial hypertension remains poorly understood. This study evaluated the influence of physical training on cardiac remodeling in untreated spontaneously hypertensive rats (SHR). Methods Four experimental groups were used: sedentary (W-SED n=27) and trained (W-EX, n=31) normotensive Wistar rats, and sedentary (SHR-SED, n=27) and exercised (SHR-EX, n=32) hypertensive rats. At 13 months old, the exercise groups underwent treadmill exercise five days a week, for four months. Echocardiogram was performed to evaluate cardiac structures and function. In vitro myocardial function was analyzed in left ventricular (LV) papillary muscle preparations. Myocardial collagen was quantified by histology and hydroxyproline concentration and antioxidant enzyme activity was assessed by spectrophotometry. NADPH oxidase activity was analyzed by lucigenin reduction. Protein expression was quantified by Western blot. Matrix metalloproteinases (MMP) activity was evaluated by zymography. Statistical analyzes: two factor ANOVA and Bonferroni or Kruskal-Wallis and Dunn tests. Results Systolic blood pressure was higher in SHR groups. Exercised groups had greater physical capacity. Frequency of heart failure features was higher in hypertensive groups than controls; SHR-EX had a lower frequency of pleural effusion and tachypnea than SHR-SED. Echocardiogram showed lower LV wall thickness, LV relative wall thickness, left atrium diameter, and relaxation time in SHR-EX than SHR-SED. Myocardial function was better in SHR-EX (positive derivative of developed tension) than SHR-SED. SHR-EX had higher antioxidant enzyme activity than SHR-SED. Lipid hydroperoxide concentration, myocyte diameters, and phosphorylated JNK and total IkB protein expression were higher in hypertensive than control groups. Hydroxyproline, malondialdehyde, NADPH oxidase activity, and protein expression of collagen III, lysyl oxidase, TIMP-1, total JNK, phosphorylated p38, phosphorylated and total p65, and phosphorylated IkB did not differ between groups. Interstitial collagen fraction, MMP-2 activity, and protein expression of total p38, and total and phosphorylated ERK were higher in SHR-SED than W-SED. Exercise reduced MMP-2 activity and phosphorylated ERK in hypertensive rats. Conclusion Physical exercise improves physical capacity, reduces the frequency of heart failure features, and attenuates cardiac remodeling in spontaneously hypertensive rats. In addition, exercise increases antioxidant enzyme activity, decreases ERK phosphorylation and MMP-2 activity, and attenuates total ERK protein expression. Acknowledgement/Funding Fapesp, CNPq, Capes and UNESP

scholarly journals Aerobic Exercise During Advance Stage of Uncontrolled Arterial Hypertension

2021 ◽  
Vol 12 ◽  
Author(s):  
Luana U. Pagan ◽  
Mariana J. Gomes ◽  
Ricardo L. Damatto ◽  
Aline R. R. Lima ◽  
Marcelo D. M. Cezar ◽  
...  
Keyword(s):  
Heart Failure ◽  
Enzyme Activity ◽  
Energy Metabolism ◽  
Protein Expression ◽  
Arterial Hypertension ◽  
Antioxidant Enzyme ◽  
Oxidase Activity ◽  
Myocardial Function ◽  
Antioxidant Enzyme Activity ◽  
Nadph Oxidase Activity

AimTo evaluate the influence of physical training on myocardial function, oxidative stress, energy metabolism, and MAPKs and NF-κB signaling pathways in spontaneously hypertensive rats (SHR), at advanced stage of arterial hypertension, which precedes heart failure development.MethodsWe studied four experimental groups: normotensive Wistar rats (W, n = 27), trained W (W-EX, n = 31), SHR (n = 27), and exercised SHR (SHR-EX, n = 32). At 13 months old, the exercise groups underwent treadmill exercise 5 days a week for 4 months. In vitro myocardial function was analyzed in left ventricular (LV) papillary muscle preparations. Antioxidant enzyme activity and energy metabolism were assessed by spectrophotometry. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity was analyzed by lucigenin reduction and protein expression by Western blot. Statistical analyzes: ANOVA and Tukey or Kruskal–Wallis and Dunn tests.ResultsSHR-EX had a lower frequency of heart failure features than SHR. Myocardial function and antioxidant enzyme activity were better in SHR-EX than SHR. Lipid hydroperoxide concentration, and phosphorylated JNK and total IkB protein expression were higher in hypertensive than control groups. Malondialdehyde, NADPH oxidase activity, total JNK, phosphorylated p38, phosphorylated and total p65 NF-κB, and phosphorylated IkB did not differ between groups. Protein expression from total p38, and total and phosphorylated ERK were higher in SHR than W. Lactate dehydrogenase and phosphorylated ERK were lower and citrate synthase and β-hydroxyacyldehydrogenase were higher in SHR-EX than SHR.ConclusionExercise improves physical capacity, myocardial function, and antioxidant enzyme activity; reduces the frequency of heart failure features and ERK phosphorylation; and normalizes energy metabolism in SHR.

scholarly journals Molybdenum application enhances antioxidant enzyme activity and COR15a protein expression under cold stress in wheat

2016 ◽  
Vol 11 (1) ◽  
pp. 5-10 ◽  
Author(s):  
Mohammed Al-Issawi ◽  
Hail Z. Rihan ◽  
Hanady Al-Shmgani ◽  
Michael P. Fuller
Keyword(s):  
Enzyme Activity ◽  
Protein Expression ◽  
Cold Stress ◽  
Antioxidant Enzyme ◽  
Antioxidant Enzyme Activity

Antioxidant Enzyme Levels Inversely Covary with Hearing Loss After Amikacin Treatment

2003 ◽  
Vol 14 (03) ◽  
pp. 134-143 ◽  
Author(s):  
James J. Klemens ◽  
Robert P. Meech ◽  
Larry F. Hughes ◽  
Satu Somani ◽  
Kathleen C.M. Campbell
Keyword(s):  
Hearing Loss ◽  
Enzyme Activity ◽  
Antioxidant Enzyme ◽  
Guinea Pigs ◽  
Auditory Brainstem ◽  
Antioxidant Enzyme Activity ◽  
Control Group ◽  
Glutathione S Transferase ◽  
Brainstem Response ◽  
The Difference

This study's purpose was to determine if a correlation exists between cochlear antioxidant activity changes and auditory function after induction of aminoglycoside (AG) ototoxicity. Two groups of five 250-350 g albino guinea pigs served as subjects. For 28 days, albino guinea pigs were administered either 200 mg/kg/day amikacin, or saline subcutaneously. Auditory brainstem response testing was performed prior to the first injection and again before sacrifice, 28 days later. Cochleae were harvested and superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase, glutathione reductase activities and malondialdehyde levels were measured. All antioxidant enzymes had significantly lower activity in the amikacin group (p ≤ 0.05) than in the control group. The difference in cochlear antioxidant enzyme activity between groups inversely correlated significantly with the change in ABR thresholds. The greatest correlation was for the high frequencies, which are most affected by aminoglycosides. This study demonstrates that antioxidant enzyme activity and amikacin-induced hearing loss significantly covary.

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