scholarly journals LC/MS profiling of flavonoid glycoconjugates isolated from hairy roots, suspension root cell cultures and seedling roots of Medicago truncatula

Metabolomics ◽  
2011 ◽  
Vol 7 (4) ◽  
pp. 604-613 ◽  
Author(s):  
Anna Staszków ◽  
Barbara Swarcewicz ◽  
Joanna Banasiak ◽  
Dorota Muth ◽  
Michał Jasiński ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Cell Cultures ◽  
Hairy Roots ◽  
Root Cell ◽  
Seedling Roots

scholarly journals Resveratrol Biosynthesis in Hairy Root Cultures of Tan and Purple Seed Coat Peanuts

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 975
Author(s):  
Ye-Eun Park ◽  
Chang-Ha Park ◽  
Hyeon-Ji Yeo ◽  
Yong-Suk Chung ◽  
Sang-Un Park
Keyword(s):  
Arachis Hypogaea ◽  
Hairy Root ◽  
Hairy Roots ◽  
Seed Coat ◽  
Biological Properties ◽  
Fresh Weight ◽  
Good Source ◽  
Plant Parts ◽  
Seedling Roots

Peanut (Arachis hypogaea) is a crop that can produce resveratrol, a compound with various biological properties, such as those that exert antioxidant, anticancer, and anti-inflammatory effects. In this study, trans-resveratrol was detected in the roots, leaves, and stems of tan and purple seed coat peanuts (Arachis hypogaea) cultivated in a growth chamber. Both cultivars showed higher levels of resveratrol in the roots than the other plant parts. Thus, both cultivars were inoculated with Agrobacterium rhizogenes, in vitro, to promote hairy root development, thereby producing enhanced levels of t-resveratrol. After 1 month of culture, hairy roots from the two cultivars showed higher levels of fresh weight than those of seedling roots. Furthermore, both cultivars contained higher t-resveratrol levels than those of their seedling roots (6.88 ± 0.21 mg/g and 28.07 ± 0.46 mg/g, respectively); however, purple seed coat peanut hairy roots contained higher t-resveratrol levels than those of tan seed coat peanut hairy roots, ranging from 70.16 to 166.76 mg/g and from 46.61 to 54.31 mg/g, respectively. The findings of this study indicate that peanut hairy roots could be a good source for t-resveratrol production due to their rapid growth, high biomass, and substantial amount of resveratrol.

Triterpene and Flavonoid Biosynthesis and Metabolic Profiling of Hairy Roots, Adventitious Roots, and Seedling Roots of Astragalus membranaceus

2015 ◽  
Vol 63 (40) ◽  
pp. 8862-8869 ◽  
Author(s):  
Yun Ji Park ◽  
Aye Aye Thwe ◽  
Xiaohua Li ◽  
Yeon Jeong Kim ◽  
Jae Kwang Kim ◽  
...  
Keyword(s):  
Hairy Roots ◽  
Metabolic Profiling ◽  
Adventitious Roots ◽  
Flavonoid Biosynthesis ◽  
Astragalus Membranaceus ◽  
Seedling Roots

scholarly journals The Lipopolysaccharide of Sinorhizobium meliloti Suppresses Defense-Associated Gene Expression in Cell Cultures of the Host Plant Medicago truncatula

2007 ◽  
Vol 143 (2) ◽  
pp. 825-837 ◽  
Author(s):  
Verena Tellström ◽  
Björn Usadel ◽  
Oliver Thimm ◽  
Mark Stitt ◽  
Helge Küster ◽  
...  
Keyword(s):  
Gene Expression ◽  
Host Plant ◽  
Medicago Truncatula ◽  
Cell Cultures ◽  
Sinorhizobium Meliloti

Cell-line-dependent sorting of recombinant phytase in cell cultures of Medicago truncatula

2009 ◽  
Vol 36 (5) ◽  
pp. 431 ◽  
Author(s):  
Pablo González-Melendi ◽  
Ana Sofia Pires ◽  
Rita Abranches
Keyword(s):  
Recombinant Protein ◽  
Medicago Truncatula ◽  
Cell Cultures ◽  
Low Cost ◽  
Protein A ◽  
Subcellular Location ◽  
Efficient System ◽  
Recombinant Product ◽  
Transgenic Lines ◽  
Recombinant Phytase

Plant cell cultures as platforms for recombinant protein production are favoured over other systems because they combine the benefits of plants (low cost of production, low biosecurity risk, conserved post-translational modifications) with those of controlled cell cultures. However, many factors that affect the correct synthesis and accumulation of the recombinant product still need to be determined; in particular, the trafficking route of the recombinant proteins is poorly understood. Suspension cell cultures of Medicago truncatula Gaertn. have been shown to offer a viable and highly efficient system for the production of a model glycoprotein – phytase from the fungus Aspergillus niger Tiegh. The present study investigated subcellular protein sorting by immunogold detection of recombinant phytase with an electron microscope in four independent Medicago cell cultures expressing phytase. Two lines contained a C-terminal KDEL targeting signal for retention in the endoplasmic reticulum (ER), and the other two did not and were expected to travel through the secretory route; a high and low expressor were examined for each variant of the protein. A differential subcellular location of phytase was found in the four transgenic lines studied. These differences account not only for the version of the recombinant protein (secreted or retained in the ER), but also for the different expression levels.

Cell Cultures and Hairy Roots as Platform for Production of High-Value Metabolites: Current Approaches, Limitations, and Future Prospects

2019 ◽  
pp. 23-57
Author(s):  
Paola Isabel Angulo-Bejarano ◽  
Juan Luis De la Fuente Jimenez ◽  
Sujay Paul ◽  
Marcos de Donato-Capote ◽  
Irais Castillo-Maldonado ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Hairy Roots ◽  
Future Prospects

scholarly journals Production of Encecalin in Cell Cultures and Hairy Roots of Helianthella quinquenervis (Hook.) A. Gray

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3231
Author(s):  
J. Mabel Hernández-Altamirano ◽  
Irene F. Ugidos ◽  
Javier Palazón ◽  
Mercedes Bonfill ◽  
Penélope García-Angulo ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Hairy Roots ◽  
Cell Suspension Cultures ◽  
Gas Chromatography Mass Spectrometry ◽  
Wild Type ◽  
Transformed Roots ◽  
Organ Cultures ◽  
Chain Reaction ◽  
Biotechnological Production ◽  
Polymerase Chain

Plant cell and organ cultures of Helianthella quinquenervis, a medicinal plant whose roots are used by the Tarahumara Indians of Chihuahua, Mexico, to relieve several ailments, were established to identify and quantify some chromenes with biological activity, such as encecalin, and to evaluate their potential for biotechnological production. Gas chromatography–mass spectrometry (GC-MS) analysis corroborated the presence of quantifiable amounts of encecalin in H. quinquenervis cell cultures (callus and cell suspensions). In addition, hairy roots were obtained through three transformation protocols (prick, 45-s sonication and co-culture), using wild type Agrobacterium rhizogenes A4. After three months, cocultivation achieved the highest percentage of transformation (66%), and a comparable production (FW) of encecalin (110 μg/g) than the sonication assay (120 μg/g), both giving far higher yields than the prick assay (19 μg/g). Stable integration of rolC and aux1 genes in the transformed roots was confirmed by polymerase chain reaction (PCR). Hairy roots from cocultivation (six months-old) accumulated as much as 1086 μg/g (FW) of encecalin, over three times higher than the cell suspension cultures. The production of encecalin varied with growth kinetics, being higher at the stationary phase. This is the first report of encecalin production in hairy roots of H. quinquenervis, demonstrating the potential for a future biotechnological production of chromenes.

scholarly journals Cell Type-Specific Imaging of Calcium Signaling in Arabidopsis thaliana Seedling Roots Using GCaMP3

2020 ◽  
Vol 21 (17) ◽  
pp. 6385
Author(s):  
William Krogman ◽  
J. Alan Sparks ◽  
Elison B. Blancaflor
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Types ◽  
Root Cell ◽  
Cauliflower Mosaic Virus ◽  
Cell Type ◽  
Root Cells ◽  
Cell Tissue ◽  
Seedling Roots ◽  
Cell Type Specific

Cytoplasmic calcium ([Ca2+]cyt) is a well-characterized second messenger in eukaryotic cells. An elevation in [Ca2+]cyt levels is one of the earliest responses in plant cells after exposure to a range of environmental stimuli. Advances in understanding the role of [Ca2+]cyt in plant development has been facilitated by the use of genetically-encoded reporters such as GCaMP. Most of these studies have relied on promoters such as Cauliflower Mosaic Virus (35S) and Ubiquitin10 (UBQ10) to drive expression of GCaMP in all cell/tissue types. Plant organs such as roots consist of various cell types that likely exhibit unique [Ca2+]cyt responses to exogenous and endogenous signals. However, few studies have addressed this question. Here, we introduce a set of Arabidopsis thaliana lines expressing GCaMP3 in five root cell types including the columella, endodermis, cortex, epidermis, and trichoblasts. We found similarities and differences in the [Ca2+]cyt signature among these root cell types when exposed to adenosine tri-phosphate (ATP), glutamate, aluminum, and salt, which are known to trigger [Ca2+]cyt increases in root cells. These cell type-targeted GCaMP3 lines provide a new resource that should enable more in depth studies that address how a particular environmental stimulus is linked to specific root developmental pathways via [Ca2+]cyt.

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