RAS-Driven Macropinocytosis of Albumin or Dextran Reveals Mutation-Specific Target Engagement of RAS p.G12C Inhibitor ARS-1620 by NIR-Fluorescence Imaging

Objectives Current diagnostic modalities for patients with peripheral artery disease (PAD) mainly focus on the macrovascular level. For assessment of tissue perfusion, near-infrared (NIR) fluorescence imaging using indocyanine green (ICG) seems promising. In this prospective cohort study, ICG NIR fluorescence imaging was performed pre- and post-revascularization to assess changes in foot perfusion. Methods ICG NIR fluorescence imaging was performed in 36 patients with PAD pre- and post-intervention. After intravenous bolus injection of 0.1 mg/kg ICG, the camera registered the NIR fluorescence intensity over time on the dorsum of the feet for 15 min using the Quest Spectrum Platform®. Time-intensity curves were plotted for three regions of interest (ROI): (1) the dorsum of the foot, (2) the forefoot, and (3) the hallux. Time-intensity curves were normalized for maximum fluorescence intensity. Extracted parameters were the maximum slope, area under the curve (AUC) for the ingress, and the AUC for the egress. The non-treated contralateral leg was used as a control group. Results Successful revascularization was performed in 32 patients. There was a significant increase for the maximum slope and AUC egress in all three ROIs. The most significant difference was seen for the maximum slope in ROI 3 (3.7%/s to 6.6%/s, p < 0.001). In the control group, no significant differences were seen for the maximum slope and AUC egress in all ROIs. Conclusions This study shows the potential of ICG NIR fluorescence imaging in assessing the effect of revascularization procedures on foot perfusion. Future studies should focus on the use of this technique in predicting favorable outcome of revascularization procedures.

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NIR fluorescence imaging of lipid drops viscosity in liver organs of diabetic mice

Dyes and Pigments ◽

10.1016/j.dyepig.2020.109120 ◽

2021 ◽

Vol 187 ◽

pp. 109120

Author(s):

Bingli Lu ◽

Junling Yin ◽

Cong Liu ◽

Weiying Lin

Keyword(s):

Fluorescence Imaging ◽

Diabetic Mice ◽

Nir Fluorescence

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Cholesterol-enriched erythrocyte-derived optical nanoparticles for NIR fluorescence imaging of intraperitoneal ovarian tumors in mice

Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XIII ◽

10.1117/12.2582797 ◽

2021 ◽

Author(s):

Chi Hua Lee ◽

Jack C. Tang ◽

Raviraj Vankayala ◽

Jenny T. Mac ◽

Taylor Hanley ◽

...

Keyword(s):

Fluorescence Imaging ◽

Ovarian Tumors ◽

Nir Fluorescence

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Near-Infrared Fluorescence Imaging and Photodynamic Therapy for Liver Tumors

Frontiers in Oncology ◽

10.3389/fonc.2021.638327 ◽

2021 ◽

Vol 11 ◽

Author(s):

Masaki Kaibori ◽

Hisashi Kosaka ◽

Kosuke Matsui ◽

Morihiko Ishizaki ◽

Hideyuki Matsushima ◽

...

Keyword(s):

Photodynamic Therapy ◽

Fluorescence Imaging ◽

Near Infrared ◽

Liver Tumors ◽

Nir Fluorescence ◽

Recent Developments ◽

Fluorescent Agent ◽

Stable Particles ◽

And Function ◽

Cancer Tissues

Surgery with fluorescence equipment has improved to treat the malignant viscera, including hepatobiliary and pancreatic neoplasms. In both open and minimally invasive surgeries, optical imaging using near-infrared (NIR) fluorescence is used to assess anatomy and function in real time. Here, we review a variety of publications related to clinical applications of NIR fluorescence imaging in liver surgery. We have developed a novel nanoparticle (indocyanine green lactosome) that is biocompatible and can be used for imaging cancer tissues and also as a drug delivery system. To date, stable particles are formed in blood and have an ~10–20 h half-life. Particles labeled with a NIR fluorescent agent have been applied to cancer tissues by the enhanced permeability and retention effect in animals. Furthermore, this article reviews recent developments in photodynamic therapy with NIR fluorescence imaging, which may contribute and accelerate the innovative treatments for liver tumors.

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Double pH-sensitive Nanotheranostics of Polypeptide Nanoparticle-Encapsulated BODIPY with Both NIR Activated Fluorescence and Enhanced Photodynamic Therapy

Journal of Materials Chemistry B ◽

10.1039/d1tb01768c ◽

2021 ◽

Author(s):

Huiping Dang ◽

Quan Cheng ◽

Youliang Tian ◽

Changchang Teng ◽

Kai Xie ◽

...

Keyword(s):

Photodynamic Therapy ◽

Tumor Microenvironment ◽

Cancer Therapy ◽

Fluorescence Imaging ◽

Intelligent Systems ◽

Ph Sensitive ◽

Nir Fluorescence

To achieve accurate fluorescence imaging-guided cancer therapy, intelligent systems with specific responsiveness to the tumor microenvironment need to be designed. Here, we have achieved both enhanced NIR fluorescence and photodynamic...

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Using Scuba for In Situ Determination of Chlorophyll Distributions in Corals by Underwater Near Infrared Fluorescence Imaging

Journal of Marine Science and Engineering ◽

10.3390/jmse8010053 ◽

2020 ◽

Vol 8 (1) ◽

pp. 53

Author(s):

Thomas Oh ◽

Jittiwat Sermsripong ◽

Barry W. Hicks

Keyword(s):

Fluorescence Imaging ◽

Large Scale ◽

Near Infrared ◽

Fluorescent Proteins ◽

Fluorescence Emission ◽

Florida Keys ◽

Nir Fluorescence ◽

Novel Method ◽

Near Infrared Fluorescence Imaging

Studies reporting quantitation and imaging of chlorophyll in corals using visible fluorescent emission in the red near 680 nm can suffer from competing emission from other red-emitting pigments. Here, we report a novel method of selectively imaging chlorophyll distributions in coral in situ using only the near infrared (NIR) fluorescence emission from chlorophyll. Commercially available equipment was assembled that allowed the sequential imaging of visible, visible-fluorescent, and NIR-fluorescent pigments on the same corals. The relative distributions of chlorophyll and fluorescent proteins (GFPs) were examined in numerous corals in the Caribbean Sea, the Egyptian Red Sea, the Indonesian Dampier Strait, and the Florida Keys. Below 2 m depth, solar induced NIR chlorophyll fluorescence can be imaged in daylight without external lighting, thus, it is much easier to do than visible fluorescence imaging done at night. The distributions of chlorophyll and GFPs are unique in every species examined, and while there are some tissues where both fluorophores are co-resident, often tissues are selectively enriched in only one of these fluorescent pigments. Although laboratory studies have clearly shown that GFPs can be photo-protective, their inability to prevent large scale bleaching events in situ may be due to their limited tissue distribution.

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3D Printing and NIR Fluorescence Imaging Techniques for the Fabrication of Implants

Materials ◽

10.3390/ma13214819 ◽

2020 ◽

Vol 13 (21) ◽

pp. 4819

Author(s):

Yong Joon Suh ◽

Tae Hyeon Lim ◽

Hak Soo Choi ◽

Moon Suk Kim ◽

Sang Jin Lee ◽

...

Keyword(s):

3D Printing ◽

Fluorescence Imaging ◽

Near Infrared ◽

Imaging Techniques ◽

The Body ◽

Monitoring Method ◽

Printing Technology ◽

3D Printing Technology ◽

Nir Fluorescence ◽

Printing Techniques

Three-dimensional (3D) printing technology holds great potential to fabricate complex constructs in the field of regenerative medicine. Researchers in the surgical fields have used 3D printing techniques and their associated biomaterials for education, training, consultation, organ transplantation, plastic surgery, surgical planning, dentures, and more. In addition, the universal utilization of 3D printing techniques enables researchers to exploit different types of hardware and software in, for example, the surgical fields. To realize the 3D-printed structures to implant them in the body and tissue regeneration, it is important to understand 3D printing technology and its enabling technologies. This paper concisely reviews 3D printing techniques in terms of hardware, software, and materials with a focus on surgery. In addition, it reviews bioprinting technology and a non-invasive monitoring method using near-infrared (NIR) fluorescence, with special attention to the 3D-bioprinted tissue constructs. NIR fluorescence imaging applied to 3D printing technology can play a significant role in monitoring the therapeutic efficacy of 3D structures for clinical implants. Consequently, these techniques can provide individually customized products and improve the treatment outcome of surgeries.

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