Abstract
Silver nanoparticles (Ag NPs) were synthesised by the reduction of Ag+ to Ag0 in the presence of enol form of flavonoids present in plant extract of Tabernaemontana Divaricate (T. Divaricate). Prepared Ag NPs were characterized using UV-Vis, XRD, HR-TEM with EDX and XPS techniques. XPS spectra exhibited peaks at 366 eV and 373 eV, which specified spin orbits for Ag 3d3/2, and Ag 3d5/2 that confirmed the formation of Ag NPs. Ag NPs were spherical in shape with an average size of 30 nm as revealed by HR-TEM and FE-SEM techniques. EDX studies verified the high purity of Ag NPs with silver 46.96 %, carbon 16.35 %, oxygen 16.22 %, nitrogen 20.25 % and sulphur 0.21%. LC-MS analysis of plant extract confirmed the qualitative presence of alkaloids, tannins, flavonoids, phenols, and carbohydrates. Prepared Ag NPs showed good photocatalytic activity towards degradation of 4-Amniopyridine with 61% degradation efficiency at optimum conditions in 2 hrs of reaction time under visible light. The number of intermediates were found were ten within the mass number of 0–450. Ag NPs synthesized using bio-extract have also showed good inactivation against Escherichia Coli (E. Coli) and Bacillus Subtilis (B. Subtilis) bacteria due to availability of free radicals.