Silver Nanoparticles from Tabernaemontana Divaricate leaf extract: Mechanism of action and Bio-application for photo degradation of 4-Aminopyridine.

Abstract Silver nanoparticles (Ag NPs) were synthesised by the reduction of Ag+ to Ag0 in the presence of enol form of flavonoids present in plant extract of Tabernaemontana Divaricate (T. Divaricate). Prepared Ag NPs were characterized using UV-Vis, XRD, HR-TEM with EDX and XPS techniques. XPS spectra exhibited peaks at 366 eV and 373 eV, which specified spin orbits for Ag 3d3/2, and Ag 3d5/2 that confirmed the formation of Ag NPs. Ag NPs were spherical in shape with an average size of 30 nm as revealed by HR-TEM and FE-SEM techniques. EDX studies verified the high purity of Ag NPs with silver 46.96 %, carbon 16.35 %, oxygen 16.22 %, nitrogen 20.25 % and sulphur 0.21%. LC-MS analysis of plant extract confirmed the qualitative presence of alkaloids, tannins, flavonoids, phenols, and carbohydrates. Prepared Ag NPs showed good photocatalytic activity towards degradation of 4-Amniopyridine with 61% degradation efficiency at optimum conditions in 2 hrs of reaction time under visible light. The number of intermediates were found were ten within the mass number of 0–450. Ag NPs synthesized using bio-extract have also showed good inactivation against Escherichia Coli (E. Coli) and Bacillus Subtilis (B. Subtilis) bacteria due to availability of free radicals.

Download Full-text

Green Synthesis, Characterization and Antibacterial Activity of Silver Nanoparticles from Capparis Spinosa Leaf Extract

Revista de Chimie ◽

10.37358/rc.21.1.8411 ◽

2021 ◽

Vol 72 (1) ◽

pp. 145-152

Author(s):

Saleh H. Salmen ◽

Eman Damra ◽

Tahani Awad Alahmadi ◽

Sulaiman Ali Alharbi

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Green Synthesis ◽

Plant Extract ◽

Leaf Extract ◽

Average Particle ◽

Surface Plasmon Resonance Peak ◽

Plasmon Resonance Peak ◽

E Coli ◽

Capparis Spinosa

The current study reports the green synthesis of silver nanoparticles (AgNPs) using Capparis spinosa leaf extract acting as a capping and reducing agent. The characterization of AgNPs was confirmed using ultraviolet-visible spectrophotometry (UV-Visible), fourier transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). The plant extract used reduces Ag+ into AgNPs within a few minutes as indicated by the changed color, from yellow to reddish-brown. The UV-vis spectrum of AgNPs appeared a characteristic surface plasmon resonance peak at 400-450 nm. FTIR spectroscopy confirmed the role of plant extract as a reducing and capping agent of silver ions. The spectra of FTIR revealed a broad transmission peaks from 3412 to 617 cm-1. An EDX analysis signal at 3 keV and weight 65.38% showed the peak to be in the silver region, a fact which was confirmed by the presence of elemental silver. Under TEM, the nanoparticles were seen to be spherical, with an average particle size of 13 nm. AgNPs showed antibacterial activity against S.epidermidis, S. aureus, MRSA and E. coli. The inhibition zones for S.epidermidis and S. aureus were 8 to 10 mm, while MRSA is 7 to 10 mm. The inhibition zone of E. coli was higher at 10 to 13 mm.

Download Full-text

GREEN SYNTHESIS AND CHARACTERIZATION OF SILVER NANO PARTICLES BY USING PSIDIUM GUAJAVA LEAF EXTRACT

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v8i5-s.2025 ◽

2018 ◽

Vol 8 (5-s) ◽

pp. 301-305

Author(s):

DR SHOBHA RANI TENKAYALA ◽

Pagadala R Sougandhi ◽

Mekala. Reddeppa ◽

Sapara Sekhar Harini ◽

R. Gangadhara

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Plant Extract ◽

Leaf Extract ◽

Silver Ions ◽

Psidium Guajava ◽

Nano Particles ◽

Ag Nps ◽

Agno3 Solution ◽

Uv Visible

In this study, rapid, simple approach was applied for synthesis of silver nanoparticles by using Psidium guajava aqueous leaf extract. The plant extract acts as both reducing agent and capping agent. The green synthesized silver nanoparticles were characterized by using physic-chemical techniques viz, UV-Visible spectroscopy, Fourier Transform Infrared Spectrophotometer [FTIR], Particle size analyser and Scanning electron microscopy. UV-Visible spectrophotometer showed absorbance peak in the range of 419nm.The compounds responsible for silver ions and the functional groups present in plant extract were identified and investigated by FTIR technique. The characterization data reveals that the particles were in crystalline in nature with an average size of 62nm. The silver nanoparticles (Ag NPs) were rapidly synthesized using aqueous extract of guava leaf with AgNO3 solution within 15min at room temperature, without the involvement of any hazardous chemicals. Keywords: Nano particles, green synthesis, Silver, Psidium guajava and reducing agents.

Download Full-text

Synthesis, characterization and photocatalytic application of Sophora mollis leaf extract mediated silver nanoparticles

Zeitschrift für Physikalische Chemie ◽

10.1515/zpch-2020-1803 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Arif Nazir ◽

Saqib Farooq ◽

Mazhar Abbas ◽

Eman A. Alabbad ◽

Hind Albalawi ◽

...

Keyword(s):

Silver Nanoparticles ◽

Green Synthesis ◽

Plant Extract ◽

Leaf Extract ◽

Catalytic Efficiency ◽

Industrial Effluents ◽

Xrd Analysis ◽

Ag Nps ◽

Concentration Method ◽

Photocatalytic Application

Abstract This study reports green synthesis and characterization of silver nanoparticles (Ag NPs) from Sophora mollis leaf extract. The use of S. mollis extract for preparation of Ag NPs was investigated using different techniques. Dark brown color indicates formation of nanoparticles. Fourier transform infra-red (FTIR) analysis revealed that plant extract act as a reducing and capping agent. Morphological aspects of Ag NPs were ascertained by means of SEM studies. Energy dispersive and FTIR spectroscopy results showed chemical composition and plant extract functionality respectively. X-ray diffraction (XRD) analysis showed particle size of 70 nm. Antibacterial activity of NPs was investigated by disc diffusion and minimum inhibitory concentration method. Antioxidant activity of NPs was shown by DPPH assay. The photo catalytic efficiency of synthesized Ag NPs was evaluated by degradation of methylene blue (MB) dye under UV irradiation. Ag NPs degraded MB dye up to 88% in 160 min. It is concluded that these NPs could be employed for degradation of toxic industrial effluents. Result proved the green synthesis of Ag NPs from S. mollis extract is clean, economical and safe method.

Download Full-text

Efficacy of Silver Nanoparticles Synthesized from Hymenocallis species (Spider Lilly) Leaf Extract as Antimicrobial Agents

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2019.12.5.4 ◽

2019 ◽

Vol 12 (5) ◽

pp. 4644-4647

Author(s):

K.K. Gupta ◽

Neha Kumari ◽

Neha Sinha ◽

Akruti Gupta

Keyword(s):

Silver Nanoparticles ◽

Leaf Extract ◽

Antimicrobial Agents ◽

Color Change ◽

Bacterial Species ◽

Antimicrobial Property ◽

Biogenic Synthesis ◽

E Coli ◽

Light Yellow ◽

Antibiotic Property

Biogenic synthesis of silver nanoparticles synthesized from Hymenocallis species (Spider Lilly) leaf extract was subjected for investigation of its antimicrobial property against four bacterial species (E. coli, Salmonella sp., Streptococcus sp. & Staphylococcus sp.). The results revealed that synthesized nanoparticles solution very much justify the color change property from initial light yellow to final reddish brown during the synthesis producing a characteristics absorption peak in the range of 434-466 nm. As antimicrobial agents, their efficacy was evaluated by analysis of variance in between the species and among the different concentration of AgNPs solution, which clearly showed that there was significant variation in the antibiotic property between the four different concentrations of AgNPs solution and also among four different species of bacteria taken under studies. However, silver nanoparticles solution of 1: 9 and 1:4 were proved comparatively more efficient as antimicrobial agents against four species of bacteria.

Download Full-text

Green Synthesized Silver Nanoparticles Immobilized on Activated Carbon Nanoparticles: Antibacterial Activity Enhancement Study and Its Application on Textiles Fabrics

Molecules ◽

10.3390/molecules26133790 ◽

2021 ◽

Vol 26 (13) ◽

pp. 3790

Author(s):

Pratama Jujur Wibawa ◽

Muhammad Nur ◽

Mukhammad Asy’ari ◽

Wijanarka Wijanarka ◽

Heru Susanto ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Activated Carbon ◽

Aloe Vera ◽

Particle Size Analysis ◽

Size Analysis ◽

E Coli ◽

Transmission Electron ◽

Average Size ◽

Green Synthesized Silver Nanoparticles

This research aimed to enhance the antibacterial activity of silver nanoparticles (AgNPs) synthesized from silver nitrate (AgNO3) using aloe vera extract. It was performed by means of incorporating AgNPs on an activated carbon nanoparticle (ACNPs) under ultrasonic agitation (40 kHz, 2 × 50 watt) for 30 min in an aqueous colloidal medium. The successful AgNPs synthesis was clarified with both Ultraviolet-Visible (UV-Vis) and Fourier Transform Infrared (FTIR) spectrophotometers. The successful AgNPs–ACNPs incorporation and its particle size analysis was performed using Transmission Electron Microscope (TEM). The brown color suspension generation and UV-Vis’s spectra maximum wavelength at around 480 nm confirmed the existence of AgNPs. The particle sizes of the produced AgNPs were about 5 to 10 nm in the majority number, which collectively surrounded the aloe vera extract secondary metabolites formed core-shell like nanostructure of 8.20 ± 2.05 nm in average size, while ACNPs themselves were about 20.10 ± 1.52 nm in average size formed particles cluster, and 48.00 ± 8.37 nm in average size as stacking of other particles. The antibacterial activity of the synthesized AgNPs and AgNPs-immobilized ACNPs was 57.58% and 63.64%, respectively (for E. coli); 61.25%, and 93.49%, respectively (for S. aureus). In addition, when the AgNPs-immobilized ACNPs material was coated on the cotton and polyester fabrics, the antibacterial activity of the materials changed, becoming 19.23% (cotton; E. coli), 31.73% (polyester; E. coli), 13.36% (cotton; S. aureus), 21.15% (polyester; S. aureus).

Download Full-text

Silver nanoparticles induce cytotoxicity, but not cell transformation or genotoxicity on Balb3T3 mouse fibroblasts

BioNanoMaterials ◽

10.1515/bnm-2013-0006 ◽

2013 ◽

Vol 14 (1-2) ◽

pp. 49-60 ◽

Cited By ~ 5

Author(s):

Francesca Broggi ◽

Jessica Ponti ◽

Guido Giudetti ◽

Fabio Franchini ◽

Vicki Stone ◽

...

Keyword(s):

Silver Nanoparticles ◽

Cell Transformation ◽

Micronucleus Test ◽

Chemical Properties ◽

Neoplastic Transformation ◽

Research Centre ◽

Ag Nps ◽

Joint Research ◽

Ic50 Values ◽

Average Size

AbstractSilver nanoparticles (Ag NPs) are one of the most common nanomaterials present in nanotechnology-based products. Here, the physical chemical properties of Ag NPs suspensions of 44 nm, 84 nm and 100 nm sizes synthesized in our laboratory were characterized. The NM-300 material (average size of 17 nm), supplied by the Joint Research Centre Nanomaterials Repository was also included in the present study. The Ag NPs potential cytotoxicity was tested on the Balb3T3 cell line by the Colony Forming Efficiency assay, while their potential morphological neoplastic transformation and genotoxicity were tested by the Cell Transformation Assay and the micronucleus test, respectively. After 24 h of exposure, NM-300 showed cytotoxicity with an IC50 of 8 µM (corresponding to 0.88 µg/mL) while for the other nanomaterials tested, values of IC50 were higher than 10 µM (1.10 µg/mL). After 72 h of exposure, Ag NPs showed size-dependent cytotoxic effect with IC50 values of 1.5 µM (1.16 µg/mL) for NM-300, 1.7 µM (1.19 µg/mL) for Ag 44 nm, 1.9 µM (0.21 µg/mL) for Ag 84 nm and 3.2 µM (0.35 µg/mL) for Ag 100 nm. None of the Ag NPs tested was able to induce either morphological neoplastic transformation or micronuclei formation.

Download Full-text

Microwave-Assisted Green Synthesis of Silver Nanoparticles Using Juglans regia Leaf Extract and Evaluation of Their Physico-Chemical and Antibacterial Properties

Antibiotics ◽

10.3390/antibiotics7030068 ◽

2018 ◽

Vol 7 (3) ◽

pp. 68 ◽

Cited By ~ 8

Author(s):

Mahsa Eshghi ◽

Hamideh Vaghari ◽

Yahya Najian ◽

Mohammad Najian ◽

Hoda Jafarizadeh-Malmiri ◽

...

Keyword(s):

Silver Nanoparticles ◽

Leaf Extract ◽

Juglans Regia ◽

Antibacterial Properties ◽

Infrared Analysis ◽

Ag Nps ◽

Stabilizing Agents ◽

Agno3 Solution ◽

Transmission Electron ◽

Vis Spectroscopy

Silver nanoparticles (Ag NPs) were synthesized using Juglans regia (J. regia) leaf extract, as both reducing and stabilizing agents through microwave irradiation method. The effects of a 1% (w/v) amount of leaf extract (0.1–0.9 mL) and an amount of 1 mM AgNO3 solution (15–25 mL) on the broad emission peak (λmax) and concentration of the synthesized Ag NPs solution were investigated using response surface methodology (RSM). Fourier transform infrared analysis indicated the main functional groups existing in the J. regia leaf extract. Dynamic light scattering, UV-Vis spectroscopy and transmission electron microscopy were used to characterize the synthesized Ag NPs. Fabricated Ag NPs with the mean particle size and polydispersity index and maximum concentration and zeta potential of 168 nm, 0.419, 135.16 ppm and −15.6 mV, respectively, were obtained using 0.1 mL of J. regia leaf extract and 15 mL of AgNO3. The antibacterial activity of the fabricated Ag NPs was assessed against both Gram negative (Escherichia coli) and positive (Staphylococcus aureus) bacteria and was found to possess high bactericidal effects.

Download Full-text

Biogenic silver nanoparticles from Trichodesma indicum aqueous leaf extract against Mythimna separata and evaluation of its larvicidal efficacy

Journal of Plant Protection Research ◽

10.1515/jppr-2017-0026 ◽

2017 ◽

Vol 57 (2) ◽

pp. 194-200 ◽

Cited By ~ 10

Author(s):

Abdul A. Buhroo ◽

Gousul Nisa ◽

Syed Asrafuzzaman ◽

Ram Prasad ◽

Razia Rasheed ◽

...

Keyword(s):

Silver Nanoparticles ◽

Leaf Extract ◽

Average Particle ◽

Particle Sizes ◽

Mythimna Separata ◽

Ag Nps ◽

Leaf Extracts ◽

Tem Analysis ◽

Vis Spectroscopy ◽

Aqueous Leaf Extract

AbstractThe present exploration is focused on the bio-fabrication of silver nanoparticles (Ag NPs) usingTrichodesma indicumaqueous leaf extract as a reducing agent. The synthesized Ag NPs were productively characterized by UV-vis spectroscopy, XRD, and TEM studies. The photosynthesis of Ag NPs was done at room temperature for 24 h and at 60°C. The green synthesis of spherical-shaped Ag NPs bio-fabricated fromT. indicumwith a face centred cubic structure showed average particle sizes of 20–50 nm, which is inconsistent with the particle size calculated by the XRD Scherer equation and TEM analysis. We further explored the larvicidal efficacy of biosynthesized Ag NPs with leaf extracts ofT. indicumagainstMythimna separata. The results showed that Ag NPs (20–50 nm) ofT. indicumpossess good larvicidal activity againstM. separatawith an LC50of 500 ppm. Thus, we can advocate that Ag NPs of 20–50 nm size extracted fromT. indicummay be considered in the pest management programme ofM. separatain future.

Download Full-text

A Surface Dielectric Barrier Discharge Plasma for Preparing Cotton-Fabric-Supported Silver Nanoparticles

Nanomaterials ◽

10.3390/nano9070961 ◽

2019 ◽

Vol 9 (7) ◽

pp. 961 ◽

Cited By ~ 3

Author(s):

Zhiyuan Fan ◽

Lanbo Di ◽

Xiuling Zhang ◽

Hongyang Wang

Keyword(s):

Silver Nanoparticles ◽

Dielectric Barrier Discharge ◽

Cotton Fabric ◽

Barrier Discharge ◽

Dbd Plasma ◽

Ag Nps ◽

Dielectric Barrier ◽

E Coli ◽

Plasma Method ◽

Surface Dielectric Barrier Discharge

Cotton-fabric-supported silver nanoparticles (Ag NPs) have aroused great attention due to their remarkable physical and chemical properties and excellent broad-spectrum antibacterial performance.In this work, a surface dielectric barrier discharge (DBD) plasma method is developed and employed to prepare cotton fabric supported Ag NPs (Ag/cotton) for the first time. UV-Vis and X-ray photoelectron spectroscopy (XPS) results confirm the formation of Ag NPs. TEM images show that the size of Ag NPs is in the range 4.8–5.3 nm. Heat-sensitive cotton fabrics are not destroyed by surface DBD plasma according to FTIR and XRDresults. Wash fastness of the Ag/cotton samples is investigated using ultrasonic treatment for 30 min and it is shown that the Ag NPs possess good adhesion to the cotton fabric according to UV-Vis spectra. Antibacterial activity of the Ag/cotton samples shows that obvious bacteriostasis loops are observed around the samples with the appearance of both Gram-negative bacterium Escherichia coli (E. coli) and Gram-positive bacterium Bacillus subtilis (B. subtilis). The average diameter of the bacteriostasis loops against both E. coli and B. subtilis becomes larger with an increasing silver loading amount.This work provides a universal, fast, simple, and environmentally-friendly cold plasma method for synthesizing Ag NPs on heat-sensitive materials at atmospheric pressure.

Download Full-text