Direct electrochemistry and enzymatic activity of hemoglobin in positively charged colloid Au nanoparticles and hemoglobin layer-by-layer self-assembly films

2007 ◽  
Vol 50 (5) ◽  
pp. 620-628 ◽  
Author(s):  
Ruo Yuan ◽  
ShuRui Cao ◽  
YaQin Chai ◽  
FengXian Gao ◽  
Qing Zhao ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Self Assembly ◽  
Au Nanoparticles ◽  
Direct Electrochemistry ◽  
Layer By Layer ◽  
Positively Charged

Multilayer composite beads constructed via layer-by-layer self-assembly for lysozyme controlled release

RSC Advances ◽  
2014 ◽  
Vol 4 (46) ◽  
pp. 24369-24376 ◽  
Author(s):  
Jiemin Zhao ◽  
Xiaoping Wang ◽  
Yanshen Kuang ◽  
Yufeng Zhang ◽  
Xiaowen Shi ◽  
...  
Keyword(s):  
Controlled Release ◽  
Self Assembly ◽  
Cross Linking ◽  
Layer By Layer ◽  
Multilayer Composite ◽  
Composite Beads ◽  
Assembly Technique ◽  
Positively Charged

Alginate (ALG)–lysozyme (LZ) beads were fabricated by a cross-linking process. Negatively charged ALG and positively charged LZ were alternately deposited on the positively charged ALG–LZ beads via a layer-by-layer (LBL) self-assembly technique.

Controllable assembly of well-defined monodisperse Au nanoparticles on hierarchical ZnO microspheres for enhanced visible-light-driven photocatalytic and antibacterial activity

Nanoscale ◽  
2015 ◽  
Vol 7 (45) ◽  
pp. 19118-19128 ◽  
Author(s):  
Yuan Wang ◽  
Hua-Bin Fang ◽  
Yan-Zhen Zheng ◽  
Rongqin Ye ◽  
Xia Tao ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Visible Light ◽  
Self Assembly ◽  
Au Nanoparticles ◽  
Layer By Layer ◽  
Visible Light Driven ◽  
Assembly Technique

An excellent visible-light-driven photocatalyst composed of well-defined monodisperse Au nanoparticles on ZnO microspheres via a layer-by-layer self-assembly technique is demonstrated.

Adsoption CD34 Antibody onto the Col/Hep Coating Film on Titanium to Improve Cytocompatibility of Titanium

2008 ◽  
Vol 47-50 ◽  
pp. 1411-1414 ◽  
Author(s):  
Jia Long Chen ◽  
Quan Li Li ◽  
Jun Ying Chen ◽  
Nan Huang
Keyword(s):  
Self Assembly ◽  
Electrostatic Interactions ◽  
Layer By Layer ◽  
Coating Film ◽  
Test Results ◽  
Cardiovascular Devices ◽  
Assembly Technique ◽  
Confluent Cell ◽  
Good Potential ◽  
Positively Charged

This study deals with improving the cytocompatibility of titanium by a coating with heparin(hep), collagen(col) and CD34 antibody. Collagen and heparin molecules were adsorbed onto a titanium surface using a layer-by-layer (LBL) self-assembly technique. The col/hep coating were formed by alternating deposition of negatively charged heparin and positively charged collagen based on electrostatic interactions. Finally the CD34 antibody was absorbed onto the surface of col/hep coating by electrostatic interactions. The chemical composition and surface topography were investigated by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The fluorescence microscopy images proved that the CD34 antibody was existed on the surface of the col/hep coating. The SEM results and the MTT test results showed that, compared to the surface of col/hep coating and the surface of titanium, the CD34 surface had better endothelial progenitor cells (EPCs) attachment and proliferation. The EPCs on the CD34 surface displayed the morphology of flat endothelium, and a confluent cell layer after cultured for 2d. This study suggested that this method maybe have good potential for surface modification of cardiovascular devices.

scholarly journals Surface Modification of Titanium with Heparin-Chitosan Multilayers via Layer-by-Layer Self-Assembly Technique

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Yao Shu ◽  
Guomin Ou ◽  
Li Wang ◽  
Jingcai Zou ◽  
Quanli Li
Keyword(s):  
Surface Modification ◽  
Self Assembly ◽  
Pure Titanium ◽  
Titanium Substrate ◽  
Titanium Implants ◽  
Layer By Layer ◽  
Assembly Technique ◽  
Titanium Surfaces ◽  
Modification Of Titanium ◽  
Positively Charged

Extracellular matrix (ECM), like biomimetic surface modification of titanium implants, is a promising method for improving its biocompatibility. In this paper chitosan (Chi) and heparin (Hep) multilayer was coated on pure titanium using a layer-by-layer (LbL) self-assembly technique. The Hep-Chi multilayer growth was carried out by first depositing a single layer of positively charged poly-L-lysine (PLL) on the NaOH-treated titanium substrate (negatively charged surface), followed by alternate deposition of negatively charged Hep and positively charged Chi, and terminated by an outermost layer of Chi. The multilayer was characterized by DR-FTIR, SEM, and AFM, and osteoblasts were cocultured with the modified titanium and untreated titanium surfaces, respectively, to evaluate their cytocompatibilityin vitro. The results confirmed that Hep-Chi multilayer was fabricated gradually on the titanium surface. The Hep-Chi multilayer-coated titanium improved the adhesion, proliferation and differentiation of osteoblasts. Thus, the approach described here may provide a basis for the preparation of modified titanium surfaces for use in dental or orthopedic implants.

Poly(diallyldimethylammonium chloride)–cellulose nanocrystals supported Au nanoparticles for nonenzymatic glucose sensing

RSC Advances ◽  
2016 ◽  
Vol 6 (8) ◽  
pp. 6436-6442 ◽  
Author(s):  
Lili Dong ◽  
Xiuqiang Zhang ◽  
Suxia Ren ◽  
Tingzhou Lei ◽  
Xiuxuan Sun ◽  
...  
Keyword(s):  
Cellulose Nanocrystals ◽  
Self Assembly ◽  
Au Nanoparticles ◽  
Glucose Sensing ◽  
The Self ◽  
Au Nps ◽  
Diallyldimethylammonium Chloride ◽  
Positively Charged

PDDA–CNCs supported Au nanohybrids, which were prepared by in-situ deposition of Au NPs, via the self-assembly between negative Au precursor and positively charged PDDA–CNCs, exhibited excellent electrochemical glucose sensing ability.

Layer-by-Layer Self-Assembly of Carbon Nanotubes with Polyelectrolytes for Biomedical Application

2013 ◽  
Vol 749 ◽  
pp. 359-361
Author(s):  
Lin Su ◽  
Pei Pei Zhao ◽  
Yun Feng Shi ◽  
Li Ting Yao ◽  
Wei Xue ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Self Assembly ◽  
Biomedical Application ◽  
Layer By Layer ◽  
3T3 Fibroblasts ◽  
Multi Walled Carbon Nanotube ◽  
Biomedical Field ◽  
Cell Biocompatibility ◽  
Nih 3T3 ◽  
Positively Charged

The layer-by-layer (LbL) self-assembly of carbon nanotube (CNT)/polyelectrolyte has been widely investigated for various applications. In this study, multi-walled carbon nanotube (MWCNT) was LbL assembled with three positively charged polyelectrolytes, which was PEI, chitosan and PDDA respectively. In order to investigate its potential application in biomedical field, cell biocompatibility was examined by examining the viability and morphologies of NIH-3T3 fibroblasts cultured on the glass coated with the MWCNT/polyelectrolyte assembly multi-layers.

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