Prediction of response of collagen-induced arthritis rats to methotrexate: An 1H-NMR-based urine metabolomic analysis

Rheumatoid arthritis (RA) is a chronic autoimmune disease with high incidence and high disability and recurrence rates. Caulophyllum robustum Maxim (C. robustum) is a traditional Chinese medicine (TCM) with main effective parts (CRME) commonly used for RA treatment. To explore the mechanism of CRME in RA, we used metabolomics to investigate the effect of CRME intervention on urine metabolism in rats with collagen-induced arthritis (CIA). CIA rats were randomly divided into normal control, CIA model, and CRME groups. A metabolomics approach, using Ultra-Performance Liquid Chromatography-Quadrupole-Time-of-Flight/Mass Spectrometry, was developed to perform urinary metabolic profiling. Differential metabolites were identified by comparing the CIA model and CRME groups. Preliminarily, 56 significant differential metabolites were identified in urine, and 20 metabolic pathways were disturbed by the CIA. The amount of 16 different metabolites changed in urine after CRME intervention. The production of these metabolites involves tryptophan, tyrosine, energy, cholesterol, and vitamin metabolism. CRME has anti-inflammatory and immunosuppressive effects in CIA model rats. By examining the endogenous metabolite levels, we identified potential CRME targets and pathways involved in the treatment of RA. The results of our metabolic studies indicate that CRME regulates amino acid, vitamin, energy, and lipid metabolism pathways to treat RA and may provide a new explanation for the anti-RA mechanism of CRME.

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1H NMR Metabolomic analysis of coffee and tea samples for the quantative determination of the main constituents

Planta Medica ◽

10.1055/s-0031-1282253 ◽

2011 ◽

Vol 77 (12) ◽

Author(s):

J Schripsema ◽

MD Vianna ◽

MA Lemos ◽

D Dagnino

Keyword(s):

1H Nmr ◽

Metabolomic Analysis

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Exercise Induced Changes in Salivary and Serum Metabolome in Trained Standardbred, Assessed by 1H-NMR

Metabolites ◽

10.3390/metabo10070298 ◽

2020 ◽

Vol 10 (7) ◽

pp. 298

Author(s):

Marilena Bazzano ◽

Luca Laghi ◽

Chenglin Zhu ◽

Enrica Lotito ◽

Stefano Sgariglia ◽

...

Keyword(s):

1H Nmr ◽

1H Nmr Spectroscopy ◽

Study Data ◽

Metabolomic Analysis ◽

Purine Derivatives ◽

Fitness Evaluation ◽

Exercise Induced ◽

Induced Changes ◽

First Time ◽

The Relationship

In the present study, data related to the metabolomics of saliva and serum in trained standardbred horses are provided for the first time. Metabolomic analysis allows to analyze all the metabolites within selected biofluids, providing a better understanding of biochemistry modifications related to exercise. On the basis of the current advances observed in metabolomic research on human athletes, we aimed to investigate the metabolites’ profile of serum and saliva samples collected from healthy standardbred horses and the relationship with physical exercise. Twelve trained standardbred horses were sampled for blood and saliva before (T0) and immediately after (T1) standardized exercise. Metabolomic analysis of both samples was performed by 1H-NMR spectroscopy. Forty-six metabolites in serum and 62 metabolites in saliva were detected, including alcohols, amino acids, organic acids, carbohydrates and purine derivatives. Twenty-six and 14 metabolites resulted to be significantly changed between T0 and T1 in serum and saliva, respectively. The findings of 2-hydroxyisobutyrate and 3-hydroxybutyrate in serum and GABA in equine saliva, as well as their modifications following exercise, provide new insights about the physiology of exercise in athletic horses. Glycerol might represent a novel biomarker for fitness evaluation in sport horses.

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1H NMR-Based Metabolomic Analysis of Liver, Serum, and Brain Following Ethanol Administration in Rats

Chemical Research in Toxicology ◽

10.1021/tx700324t ◽

2008 ◽

Vol 21 (2) ◽

pp. 408-420 ◽

Cited By ~ 38

Author(s):

Peter C. Nicholas ◽

Daniel Kim ◽

Fulton T. Crews ◽

Jeffrey M. Macdonald

Keyword(s):

1H Nmr ◽

Ethanol Administration ◽

Metabolomic Analysis

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Metabolomic Analysis for Compositional Differences of Bush Tea (Athrixia phylicoides DC.) Subjected to Seasonal Dynamics

Agronomy ◽

10.3390/agronomy10060892 ◽

2020 ◽

Vol 10 (6) ◽

pp. 892

Author(s):

Keletso C. Mohale ◽

Olusola Bodede ◽

Hintsa T. Araya ◽

Fhatuwani N. Mudau

Keyword(s):

South African ◽

1H Nmr ◽

Essential Amino Acids ◽

Proton Nuclear Magnetic Resonance ◽

Metabolomic Analysis ◽

Tea Leaves ◽

Herbal Tea ◽

Primary Metabolites ◽

Different Seasons ◽

Athrixia Phylicoides

Bush tea (Athrixia phylicoides DC.) is a South African indigenous herbal tea rich in secondary metabolites with medicinal significance. However, studies on the effects of seasonal changes on bush tea and quantification of its metabolites using untargeted approach have not been explored. Therefore, this study’s objective was to investigate the effects of seasonal variation on the metabolites of bush tea using LC-MS/MS (Liquid Chromatography with Tandem Mass Spectrometry) and 1H NMR (High-Resolution Proton Nuclear Magnetic Resonance) techniques. Bush tea leaves were plucked once in each season namely, autumn (March to May), winter (June to August), spring (September to November), and summer (December to February). The primary metabolites including non-essential amino acids, organic acids, and vitamins in bush tea were predominantly elevated during summer and spring. The 1H NMR-based metabolomic analysis revealed that bush tea metabolome was strongly affected by seasonal variations, using the partial least squares-discriminant analysis (PLS-DA) which demonstrated four distinct groups of seasons. Similarly, the 1H NMR based metabolic profiling of bush tea subjected to different seasons resulted in putative annotation of six phenolic compounds which included rutin, 4-(hydroxyphenyl) propyl coumarate, caffeic acid, hymenoxin, quercetin, and kaempferol. The phenolics and primary metabolites differed remarkably per season with enhanced accumulation observed in the summer and spring harvested bush tea. The LC-MS and 1H NMR metabolomics analysis suggests that summer and spring can be recommended as preferred plucking seasons for bush tea leaves and twigs.

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